期刊文献+
共找到273篇文章
< 1 2 14 >
每页显示 20 50 100
Carboxymethyl-β-cyclodextrin for Chiral Separation of Amino Acids Derivatized with Fluorescene-5-isothiocyanate by Capillary Electrophoresis and Laser-induced Fluorescence Detection 被引量:1
1
作者 Yu Yun CHEN Wei WANG +1 位作者 Wei Ping YANG Zhu Jun ZHANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第1期112-114,共3页
A method using carboxymethyl-b-cyclodextrin (CM-b-CD) as selector for chiral separation of amino acids by capillary electrophoresis and laser-induced fluorescence detection was studied. Resolution was better than tha... A method using carboxymethyl-b-cyclodextrin (CM-b-CD) as selector for chiral separation of amino acids by capillary electrophoresis and laser-induced fluorescence detection was studied. Resolution was better than that obtained by b-CD or HP-b-CD. 展开更多
关键词 chiral separation amino acids capillary electrophoresis DERIVATIZATION CM-b-CD.
下载PDF
Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography(2019–2022) 被引量:1
2
作者 Xinyu Li Qianjie Ma +2 位作者 Xiangtai Zheng Qin Chen Xiaodong Sun 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2023年第4期323-339,共17页
Capillary electrochromatography(CEC)plays a significant role in chiral separation via the double separation principle,partition coefficient difference between the two phases,and electroosmotic flow-driven separation.G... Capillary electrochromatography(CEC)plays a significant role in chiral separation via the double separation principle,partition coefficient difference between the two phases,and electroosmotic flow-driven separation.Given the distinct properties of the inner wall stationary phase(SP),the separation ability of each SP differs from one another.Particularly,it provides large room for promising applications of open tubular capillary electrochromatography(OT-CEC).We divided the OT-CEC SPs developed over the past four years into six types:ionic liquids,nanoparticle materials,microporous materials,biomaterials,non-nanopolymers,and others,to mainly introduce their characteristics in chiral drug separation.There also added a few classic SPs that occurred within ten years as supplements to enrich the features of each SP.Additionally,we discuss their applications in metabolomics,food,cosmetics,environment,and biology as analytes in addition to chiral drugs.OT-CEC plays an increasingly significant role in chiral separation and may promote the development of capillary electrophoresis(CE)combined with other instruments in recent years,such as CE with mass spectrometry(CE/MS)and CE with ultraviolet light detector(CE/UV). 展开更多
关键词 capillary electrochromatography Open tubular capillary chiral separation Stationary phase capillary electrophoresis with mass spectrometry(CE/MS)
下载PDF
Chiral Separation of Chlorpheniramine by Capillary Electrophoresis *L
3
作者 卢光菊 顾峻岭 +1 位作者 张健 傅若农 《Journal of Beijing Institute of Technology》 EI CAS 1999年第1期61-65,共5页
Aim To study the chiral separation of chlorpheniramine(Chp) by using a mixture of β CD and glutamate β CD as the chiral additive in CE. Methods Mixture of β cyclodextrin and glutamate β cyc... Aim To study the chiral separation of chlorpheniramine(Chp) by using a mixture of β CD and glutamate β CD as the chiral additive in CE. Methods Mixture of β cyclodextrin and glutamate β cyclodextrin was used as a chiral selector to the separation of chlorpheniramine. The effects of the concentrations of β cyclodextrin and glutamate β cyclodextrin, the voltage and the pH of buffers on the separation of chlorpheniramine were studied. Results The chiral recognization of the mixed chiral selector was better than that of a single selector. At pH below 4, the pH of buffers did not affect the separation and the separation of chlorpheniramine had good reproducibility. Conclusion Using a mixture of β CD and Glu β CD as chiral selector is useful for the enantioseparation of Chp in CE. 展开更多
关键词 capillary electrophoresis enantiomer separation cyclodextrin additives CHLORPHENIRAMINE
下载PDF
Separation of chiral drugs with β-CD phosphate by capillary electrophoresis
4
作者 Xue Qin REN1 Xiao Dan SU1 +2 位作者 Al Jin HUANG1 Yi Liang SUN1 Zeng Pei SUNZ2 (1 Department of Chemistry, Peking University, Beijing 100871 2 National Institute for the Control of pharmaceutical and Biological Products, Beijing 100050) 《Chinese Chemical Letters》 SCIE CAS CSCD 2000年第2期153-156,共4页
β-Cyclodextrin phosphate (β-CD-phosphate) was used as a selector for separating chiral drugs by capillary electrophoresis (CE). A solution comprising of 120 mmol/L Britton Robinson buffer (BRB) containing 10 mmol/L ... β-Cyclodextrin phosphate (β-CD-phosphate) was used as a selector for separating chiral drugs by capillary electrophoresis (CE). A solution comprising of 120 mmol/L Britton Robinson buffer (BRB) containing 10 mmol/L 0-CD phosphate with the pH adjusted to 7.0 was used as the background electrolyte (BGE), and a small amount of analyte was injected (600v/1s). Triethylamine, diethylamine, triethanolamine, diethanolamine, Tris added as modifier were compared. Isoprenaline, methoxamine, oxprenolol, practolol were successfully resolved. 展开更多
关键词 capillary electrophoresis chiral separation β-cyclodextrin-phosphate ISOPRENALINE METHOXAMINE oxprenolol practolol.
下载PDF
Chiral Separation of N-Benzoyl Phenylalanine Methyl Ester by Nonaqueous Capillary Electrophoresis
5
作者 Yi LI Lun Jia XIE +1 位作者 Hu Wei LIU Wen Ting HUA(Department of Chemistry, Peking University, Beijing 100871) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第4期303-306,共4页
A successful chiral separation of N-benzoyl phenylalanine methyl ester has been achieved by nonaqueous capillary electrophoresis (NACE) using P-CD as chiral selector in formamide (FA). Some experimental parameters inf... A successful chiral separation of N-benzoyl phenylalanine methyl ester has been achieved by nonaqueous capillary electrophoresis (NACE) using P-CD as chiral selector in formamide (FA). Some experimental parameters influencing the chiral separation such as concentration of P-CD, ionic strength and apparent pH (pH*) are discussed. 展开更多
关键词 nonaqueous capillary electrophoresis chiral separation N-benzoyl phenylalanine methyl ester
下载PDF
Carboxymethyl-β-Cyclodextrin for Chiral Separation of Basic Drugs by Capillary Elcctrophoresis
6
作者 Wei WANG Xiao Yun FU Yao Zu CHEN (Analytical and Testing Center, Zhejiang University. Hangzhou 310027)(State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第10期831-832,共2页
Four basic drugs were separated by capillary electrophoresis (CE) usingcarboxymethyl-β-yclodextrin (CM-0-CD) as chiral selector. The effects of CM-β-CDconmptration, applied voltage. operation temperature were invest... Four basic drugs were separated by capillary electrophoresis (CE) usingcarboxymethyl-β-yclodextrin (CM-0-CD) as chiral selector. The effects of CM-β-CDconmptration, applied voltage. operation temperature were investigated. Under the selectedconditions. norephedrine. ofloxacin. terbutaline and chlorpheniramine were enantiomerallybaieline-separated using H3PO4-triethanolamine buffer at PH 3 containing 10, 5.5, 2. 2mmol/LCM-β-CD respectively. 展开更多
关键词 capillary electrophoresis chiral separation carboxymethyl-β-cyclodextrin. basicdrugs.
下载PDF
Method Development of Enantiomer Separations by Affinity Capillary Electrophoresis,Cyclodextrin Electrokinetic Chromatography and Capillary Electrophoresis-Mass Spectrometry 被引量:8
7
作者 TANAKA Yoshihide (Department of Analytical Chemistry, Nippon Boehringer Ingelheim Co. Ltd., 3 10 1, Yato, Kawanishi, Hyogo 666 0193, Japan) 《色谱》 CAS CSCD 北大核心 2002年第4期317-327,共11页
Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affini... Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affinity CE) with some proteins and by cyclodextrin electrokinetic chromatography (CDEKC) with some charged cyclodextrins (CDs). Many successful enantiomer separations are demonstrated from our study in this review article. In the enantiomer separations by affinity CE, the deterioration of detection sensitivity was observed under high concentration of the protein in running solutions. The partial filling technique was practically useful to solve the serious problem. It allowed operation at high protein concentrations, such as 500 μmol/L, without the detection problem. Charged CDs had several advantages for the enantiomer separations over neutral ones. Strong electrostatic interactions between a charged CD and oppositely charged analytes should be effective for the formation of the complex. A large difference in electrophoretic mobility between the free analyte and the inclusion complex should also enhance the enantiomeric resolution. In CE mass spectrometry (CE MS), the partial filling technique was applied to avoid the introduction of nonvolatile chiral selectors into the CE MS interface. By replacing the nonvolatile electrolytes in the running buffer by volatile ones, the separation conditions employed in CE with the UV detection method could be transferred to CE MS. 展开更多
关键词 对映体 分离 亲和毛细管电泳 环糊精 电动色谱 毛细管电泳 质谱 研究进展
下载PDF
Investigation of Mixed Chiral Selectors of Different Metal Ion-L-Alanine Complex and β-Cyclodextrin on the Chiral Separation of Dansyl Amino Acids with Capillary Electrophoresis 被引量:3
8
作者 郑志侠 屈锋 林金明 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2003年第11期1478-1484,共7页
Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtaine... Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtained by using either Mn(II)- L -alanine complex or β -CD alone. The effects of separation parameters,such as pH value of buffer solution,capillary temperature,the concentration of Mn(II)- L -alanine complex,the types of CD and ligand on the migration times and resolutions were investigated. Six different transition metal complexes, Cu(II), Zn(II),Co(II),Ni(II),Hg(II) and Cd(II)- L -alanine complexes have been employed and compared with Mn(II) complex. Differences in retention and selectivity were found. The substitution of Cu(II),Zn(II),Co(II) and Ni(II) for Mn(II) resulted in a better chiral resolution while Hg(II) and Cd(II) showed poorer resolution abilities. The chiral separation mechanism was also discussed briefly. 展开更多
关键词 chiral separation ligand-exchange capillary electrophoresis dansyl amino acids Β-CYCLODEXTRIN
原文传递
Separation of chiral drugs with sulfobutylether-β-cyclodextrin bycapillary zone electrophoresis 被引量:1
9
作者 Jun Ying LIU Yi Yang DONG +4 位作者 Tian Song WANG Hu Wei LIU Al Jin HUANG yi Liang SUNI Zeng Pei SUN(Department of Chemistry. Peking University. Beijing 100871)(Natlonal Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第1期39-42,共4页
Sulfobutylether-β-cyclodextrin(SBE-β-CD)was used as a chiral selector tor separatingten chlral drugs with resolution 1.2 by capillary zone electrophoresls(CZE), The backgroundelectrolylc solution compris... Sulfobutylether-β-cyclodextrin(SBE-β-CD)was used as a chiral selector tor separatingten chlral drugs with resolution 1.2 by capillary zone electrophoresls(CZE), The backgroundelectrolylc solution comprised of 120 mmol/L Britton-Robinson buffer(BRB) containing1 ~10mmol/L SBE-β-CD with the pH value adjusted from 5.0-6.8. Five of the drugs were better resolvedthan those previously reported with neutral CDs. 展开更多
关键词 chiral separation sulfobutylether-β-cyclodextrin capillary zone electrophoresis anlsodamlne bencynonate ECONAZOLE ESMOLOL glycopynonate lobeline METHOXAMINE PHENYLEPHRINE plnacidll TERAZOSIN
下载PDF
Chiral Separation of Esmolol and Terazosin by Cyclodextrinmodified Capillary Zone Electrophoresis
10
作者 Yi Yang Dong Xue Qin REN +2 位作者 Ai Jin HUANG Yi Liang SUN Zeng Pei SUN(Department of Chendstry, Peking University, Beijing 10087)(National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050) 《Chinese Chemical Letters》 SCIE CAS CSCD 1998年第6期565-568,共4页
Cyclodextrin-modified CZE was applied to the chiral separation of two basic drugs, i.e., esmolol and terazosin. Selector screening and concentration optimization experiments were performed. Resolution 3.1 for esmolol,... Cyclodextrin-modified CZE was applied to the chiral separation of two basic drugs, i.e., esmolol and terazosin. Selector screening and concentration optimization experiments were performed. Resolution 3.1 for esmolol, 1.2 for terazosin were achieved when using 60 mmol/L gamma-CD and 60 mmoL/L DM-P-CD, respectively, in 50 mmol/L pH 2.5 sodium phosphate buffer. 展开更多
关键词 capillary zone electrophoresis chiral separation CYCLODEXTRIN ESMOLOL TERAZOSIN
全文增补中
A Novel Polybrene/Chondroitin Sulfate C Double Coated Capillary and Its Application in Capillary Electrophoresis 被引量:2
11
作者 杜迎翔 HONDA +5 位作者 Susumub TAGA Atsushib 刘文英 SUZUKI Shigeo 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2002年第12期1557-1565,1462,共9页
A new capillary coated by double polymer, polybrene/chondroitin sulfate C (P/CC), was developed using a simple procedure. The P/CC double coated capillary showed long lifetime, strong chemical stability and good repro... A new capillary coated by double polymer, polybrene/chondroitin sulfate C (P/CC), was developed using a simple procedure. The P/CC double coated capillary showed long lifetime, strong chemical stability and good reproducibility. It endured during more than 100 replicated analyses and was also tolerant to HCl (1 mol/L), NaOH (0.01 mol/L), CH3OH and CH3CN. The P/CC double coated capillary can be applied to basic drug analyses. The adsorption of basic drugs to the capillary wall was suppressed and the peak tailing greatly decreased. The use of the P/CC double coated capillary allowed excellent separation of the enantiomers of some basic drugs by using chondroitin sulfate C as the chiral selector, and the peak symmetry of basic drugs was further improved under these conditions. 展开更多
关键词 capillary electrophoresis anionic capillary coating basic drug chiral separation chondroitin sulfate C
全文增补中
深共晶溶剂协同羧甲基-β-环糊精促进美托洛尔的毛细管电泳手性分离
12
作者 李小倩 夏之宁 《色谱》 CAS CSCD 北大核心 2024年第4期327-332,共6页
手性药物的对映体理化性质极其相似,而药理、毒理作用常常存在显著差异,因此探索提高对映体分离性能的创新策略对手性分离具有重要意义。以往关于毛细管电泳(CE)中β-受体阻断药美托洛尔(MET)对映体分离的研究主要集中在仅添加手性选择... 手性药物的对映体理化性质极其相似,而药理、毒理作用常常存在显著差异,因此探索提高对映体分离性能的创新策略对手性分离具有重要意义。以往关于毛细管电泳(CE)中β-受体阻断药美托洛尔(MET)对映体分离的研究主要集中在仅添加手性选择剂环糊精(CD)及其衍生物,而对于在CD的基础上添加辅助添加剂的研究较少。本研究将3种深共晶溶剂(DESs)氯化胆碱-葡萄糖、氯化胆碱-果糖和乳酸-葡萄糖作为辅助添加剂,用于MET的CE手性分离,推测了DES对基于CD的毛细管电泳分离MET对映体的协同作用。首先考察了CD种类、CD浓度、缓冲液pH值和缓冲液浓度对MET分离的影响,优化得到了最优条件(15 mmol/L羧甲基-β-环糊精、pH=3.0、40 mmol/L磷酸盐缓冲液)。其次制备了3种DESs作为添加剂,研究不同DESs及其质量分数对手性分离的作用,最终确定了最佳条件:采用质量分数1.5%的氯化胆碱-果糖型DES,此时MET的分离度从不添加DES时的1.30提升至2.61,实现了基线分离。最后对分离效果以及机理进行了推测。本文所建立的MET对映体手性分离分析方法不仅对提高手性药物质量、保证临床用药安全有效具有重要意义,还有助于将DES协同用于CD衍生物毛细管电泳手性分离的进一步研究与发展。 展开更多
关键词 毛细管电泳 协同作用 手性分离 深共晶溶剂 美托洛尔
下载PDF
毛细管电泳拆分手性药物的实验教学改革与探索
13
作者 冉瑞雪 董林毅 +2 位作者 赵文楠 王涎桦 李荣珊 《实验室科学》 2023年第5期48-51,55,共5页
高效毛细管电泳是进行手性异构体拆分的常规仪器,也是药学专业的学生必须掌握的大型仪器之一。通过设计药学案例介绍了高效毛细管电泳的分离模式、影响分离的因素及手性分离策略,探索了pH值、手性添加剂的浓度及手性添加剂的种类对佐匹... 高效毛细管电泳是进行手性异构体拆分的常规仪器,也是药学专业的学生必须掌握的大型仪器之一。通过设计药学案例介绍了高效毛细管电泳的分离模式、影响分离的因素及手性分离策略,探索了pH值、手性添加剂的浓度及手性添加剂的种类对佐匹克隆对映体分离的影响。该实验引入了新仪器,增加了学生的实验兴趣,锻炼了学生的科研思维能力,该实验可以在本科药学专业仪器分析实验中推广使用。 展开更多
关键词 毛细管电泳 手性拆分 佐匹克隆 实验教学
下载PDF
Investigation on chiral capillary columns and their application in separation of enantiomers
14
作者 ZHOU, Liang-Mo LOU, Xian-Wen +2 位作者 LIU, You-Qin WANG, Qing-Hai ZHU, Dao-Qian Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012 《Chinese Journal of Chemistry》 SCIE CAS CSCD 1992年第5期430-433,共0页
Six chiral diamide stationary phases (CSPs), namely N-(3-carbobenzoxypropionyl)-L- Val-tert-butylamide (CSP-1), N-undecenoyl-L-Val-S-α-phenylethylamide (CSP-2), N-undecenoyl-L- Val-R-α-phenylethylamide (CSP-3), OV-2... Six chiral diamide stationary phases (CSPs), namely N-(3-carbobenzoxypropionyl)-L- Val-tert-butylamide (CSP-1), N-undecenoyl-L-Val-S-α-phenylethylamide (CSP-2), N-undecenoyl-L- Val-R-α-phenylethylamide (CSP-3), OV-225-L-Val-tert-butylamide (CSP-4), XE-60-L-Val-tert- butylamide (CSP-5) and polycyanoethyl vinyl siloxane-L-Val-tert-butylamide (CSP-6), were inves- tigated and CSP-6 was crosslinked within narrow bore (70 μm) fused silica capillary columns. The separation of amino acid enantiomers on this narrow bore column by gas chromatography (GC) is illustrated. 展开更多
关键词 CSP Investigation on chiral capillary columns and their application in separation of enantiomers
全文增补中
含有氨基和羧基的β-环糊精衍生物合成及性能测试 被引量:24
15
作者 戴荣继 张姝 +3 位作者 李方 靳慧 顾峻岭 傅若农 《北京理工大学学报》 EI CAS CSCD 1998年第2期159-164,共6页
目的合成含有氨基和羧基的水溶性β-环糊精衍生物并测试其性能,这些化合物用作毛细管电泳添加剂.方法将β-环糊精与含有氨基的化合物在碱性条件下用环氧氯丙烷连接起来.结果合成了七(2,6-二-O-羧甲基)-β-环糊精(羧甲... 目的合成含有氨基和羧基的水溶性β-环糊精衍生物并测试其性能,这些化合物用作毛细管电泳添加剂.方法将β-环糊精与含有氨基的化合物在碱性条件下用环氧氯丙烷连接起来.结果合成了七(2,6-二-O-羧甲基)-β-环糊精(羧甲基-β-CD)、七{2,6-二-O-[3-(2-氨基乙氨基)-2-羟丙基]}-β-环糊精(乙二胺-β-CD)、七{2,6-二-O-[3-(1,3-二羧基丙氨基)-2-羟丙基]}-β-环糊精(谷氨酸-β-CD)、七{2,6-二-O-[3-羧甲基氨基)-2-羟丙基〗}-β-环糊精(甘氨酸-β-CD)、七{2,6-二-O-[3-(1,2-二羧基乙氨基)-2-羟丙基]}-β-环糊精(天冬氨酸-β-CD)、七{2,6-二-O-[3-氨基-2-羟丙基)q-β-环糊精(氨基-β-CD)、七{2,6-二-O-[3-(2-羟基乙氨基)-2-羟丙基]}-β-环糊精(乙醇氨-β-CD)等七种含有氨基和羧基的β-环糊精衍生物,结果表明它们具有良好的水溶性.将羧甲基-β-CD和谷氨酸-β-CD用作毛细管电泳添加剂分离了异丙嗪和扑尔敏两种手性异构体,得到了较好的分离结果.结论将氨基和羧基引入环糊精后可极大改善β-环糊精的水? 展开更多
关键词 Β-环糊精 毛细管电泳 氨基 羧基 分离 环糊精
下载PDF
毛细管电泳法手性拆分合成药物氨氯地平及其中间体 被引量:10
16
作者 李保会 杨更亮 +2 位作者 王德先 张哲峰 陈义 《色谱》 CAS CSCD 北大核心 2002年第4期338-340,共3页
建立了毛细管电泳手性拆分氨氯地平药物中间体的方法 ,并同时拆分了氨氯地平。考察了不同手性拆分试剂对手性选择性的影响 ,其中羧甲基 β 环糊精 (CM β CD)能够给出满意的拆分结果。在以CM β CD为手性拆分试剂的基础上 ,还考察了各... 建立了毛细管电泳手性拆分氨氯地平药物中间体的方法 ,并同时拆分了氨氯地平。考察了不同手性拆分试剂对手性选择性的影响 ,其中羧甲基 β 环糊精 (CM β CD)能够给出满意的拆分结果。在以CM β CD为手性拆分试剂的基础上 ,还考察了各种因素诸如流动相的pH值、环糊精的浓度以及电压对分离的影响。最佳拆分条件为 :30mmol/L磷酸盐 +5 0mmol/LCM β CD(pH 6 12 )。在此条件下 ,药物中间体及氨氯地平的分离度分别为 1 5 5和 1 73。 展开更多
关键词 合成药物 毛细管电泳法 手性分离 氨氯地平 对映中间体 高血压治疗药物 手性药物
下载PDF
非水毛细管电泳对9种手性药物的拆分 被引量:26
17
作者 初永宝 蒋文强 +1 位作者 崔凤霞 李关宾 《色谱》 CAS CSCD 北大核心 2003年第2期138-142,共5页
用非水毛细管电泳成功地分离了洛贝林、托吡卡胺、普萘洛尔、阿替洛尔、环扁桃酯、沙丁胺醇、苯海索、芬氟拉明和异丙嗪等9种手性化合物。以甲酰胺为介质、柠檬酸 三羟甲基氨基甲烷(Tris)为电解质、在254nm波长下紫外检测。对手性选择... 用非水毛细管电泳成功地分离了洛贝林、托吡卡胺、普萘洛尔、阿替洛尔、环扁桃酯、沙丁胺醇、苯海索、芬氟拉明和异丙嗪等9种手性化合物。以甲酰胺为介质、柠檬酸 三羟甲基氨基甲烷(Tris)为电解质、在254nm波长下紫外检测。对手性选择剂的类型及浓度、背景电解质的pH值以及离子强度等因素对分离的影响做了系统的研究,最后确立了9种手性药物的最佳分离条件。实验发现,不同的手性选择剂对手性药物具有不同的分离选择性;每一种选择剂都存在一个相对水相体系较高的最佳浓度值而使分离效果最佳;在非水体系中,最佳pH值要比在水相体系中高;其分离效率也是随着体系中电泳介质离子强度的增大而升高。 展开更多
关键词 手性药物 非水毛细管电泳 手性拆分 甲酰胺 分离
下载PDF
高效毛细管电泳法同时测定维拉帕米和去甲维拉帕米对映体的血浆浓度 被引量:14
18
作者 芮建中 周晓东 +3 位作者 凌树森 袁倚盛 相秉仁 安登魁 《药学学报》 CAS CSCD 北大核心 1998年第7期517-522,共6页
用高效毛细管电泳中最常用的毛细管区带电泳模式,以三甲基β环糊精(TMβCD)为手性选择剂,同时测定维拉帕米和去甲维拉帕米对映体的血浆浓度。手性测定条件:手性拆分缓冲液为含60mmol·L-1TMβCD... 用高效毛细管电泳中最常用的毛细管区带电泳模式,以三甲基β环糊精(TMβCD)为手性选择剂,同时测定维拉帕米和去甲维拉帕米对映体的血浆浓度。手性测定条件:手性拆分缓冲液为含60mmol·L-1TMβCD(pH25),浓度60mmol·L-1的磷酸盐缓冲液;毛细管柱为30cm×75μm(ID)的聚丙烯酰胺涂渍柱;选择电迁移方式进样,电压12kV,时间7s;运行电压14kV;紫外检测波长200nm;设柱温为20℃;12min内完成检测。各对映体检测的线性范围为250ng·ml-1~3000ng·ml-1,准确度和精密度达到生物样品分析的要求。用本法测定健康志愿者口服和静滴VPM后各对映体的血浆浓度,结果良好。 展开更多
关键词 高效毛细管电泳 维拉帕米 去甲维拉帕米 手性
下载PDF
毛果芸香碱对映体的毛细管电泳手性分离 被引量:11
19
作者 侯经国 王柱命 +2 位作者 何天稀 杜新贞 高锦章 《分析测试学报》 CAS CSCD 北大核心 2004年第1期64-66,共3页
采用 β_环糊精及其衍生物作为手性选择剂对毛果芸香碱对映体进行了分离 ,研究了环糊精类型和浓度对分离的影响 ,同时考察了背景电解质 pH、操作电压和温度等因素对对映体分离的影响 ;结果表明 ,采用羟丙基_β_环糊精 (HP_β_CD)可以使... 采用 β_环糊精及其衍生物作为手性选择剂对毛果芸香碱对映体进行了分离 ,研究了环糊精类型和浓度对分离的影响 ,同时考察了背景电解质 pH、操作电压和温度等因素对对映体分离的影响 ;结果表明 ,采用羟丙基_β_环糊精 (HP_β_CD)可以使毛果芸香碱对映体达到基线分离 ,优化条件下手性分离度可达2.79,为此类药物提供了一种简便、快速的毛细管电泳手性分离分析方法。 展开更多
关键词 毛果芸香碱 对映体 毛细管电泳 手性分离 Β-环糊精 眼科药物
下载PDF
水溶性β-环糊精衍生物的合成及其在毛细管电泳中的应用 被引量:8
20
作者 戴荣继 张姝 +3 位作者 李方 靳慧 顾峻岭 傅若农 《化学学报》 SCIE CAS CSCD 北大核心 1998年第6期594-599,共6页
研究了七(2,6-二-O-羧甲基)-β-环糊精(羧甲基-β-CD和七{2,6-二-O[3-(1,3-二羧基丙氨基)-2-羟丙基]}-β-环糊精(谷氨酸-β-CD)两种水溶性环糊精衍生物的合成及在毛细管电泳中的应用,结果表明它们具有较好的水溶性,并且对异丙嗪和扑尔... 研究了七(2,6-二-O-羧甲基)-β-环糊精(羧甲基-β-CD和七{2,6-二-O[3-(1,3-二羧基丙氨基)-2-羟丙基]}-β-环糊精(谷氨酸-β-CD)两种水溶性环糊精衍生物的合成及在毛细管电泳中的应用,结果表明它们具有较好的水溶性,并且对异丙嗪和扑尔敏有较好的分离效果. 展开更多
关键词 CD 拆分 毛细管电泳 手性分离 异丙嗪 扑尔敏
下载PDF
上一页 1 2 14 下一页 到第
使用帮助 返回顶部