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Combining Transcriptomics and Metabolomics to Uncover the Effects of High-Energy Lithium-Ion Beam Irradiation on Capsicum annuum L.
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作者 Libo Xie Xue Wang +5 位作者 Luxiang Liu Chunmei Xu Yongdun Xie Hongchun Xiong Xinchun Han Mu Guo 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第11期2947-2964,共18页
Hot pepper(Capsicum annuum L.)is consumed as one of the oldest domesticated crops all over the world.Although mutation breeding using radiation has been performed in hot peppers,little is known about the comparative a... Hot pepper(Capsicum annuum L.)is consumed as one of the oldest domesticated crops all over the world.Although mutation breeding using radiation has been performed in hot peppers,little is known about the comparative analysis of mutagenic effects at the molecular level by ion beam irradiation.To comprehend the response mechanism of hot pepper to the ion beam,we used a mutant with favorable economic characteristics induced by lithium-ion beam irradiation to investigate the biological effects.The results indicated that the lithium-ion beam had a positive effect on important agronomic traits,particularly yield unit,but had a negligible effect on the photosynthetic rate of hot pepper,with a specific influence on chlorophyll b rather than chlorophyll a.By RNA-Seq analysis,671 up-regulated and 376 down-regulated genes were identified as differentially expressed genes(DEGs)between irradiated and unirradiated hot pepper.Based on GO and KEGG network analysis,the auxin metabolic process was the common pathway in these two networks.A total of 118 potential reactive oxygen species(ROS)scavenging genes and 262 signal transduction genes were identified,suggesting a balance between antioxidant enzymes and enhanced ROS transduction.The amounts of 15 metabolite,involved in GABA pathways,secondary metabolism,carbohydrate metabolism,shikimate pathways,TCA cycles,nitrogen metabolism,glycerol metabolism and acetate pathways,were significantly changed in the ion beam irradiated sample.These results highlighted that the enriched pathways could play important roles in response to ion beam irradiation in hot pepper plants.In summary,these data provide valuable information for future research on ion beam irradiation and genomic studies in hot pepper. 展开更多
关键词 capsicum annuum l PHYSIOlOGY TRANSCRIPTOMICS metabolomics lithium-ion beam
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五彩椒(Capsicum annuum L.)的组织培养研究 被引量:5
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作者 李凤兰 刘荣梅 +1 位作者 胡国富 胡宝忠 《东北农业大学学报》 CAS CSCD 2008年第11期62-65,共4页
以抗寒性较强的五彩樱桃椒为试验材料,对观赏椒的组织培养进行了研究。试验表明,最佳外植体为茎尖,茎尖的最佳消毒时间为3min;最佳诱导培养基为MS+6-BA5mg·L-1+NAA1mg·L-1;最佳分化培养基为MS+6-BA5mg·L-1+NAA0.1mg·... 以抗寒性较强的五彩樱桃椒为试验材料,对观赏椒的组织培养进行了研究。试验表明,最佳外植体为茎尖,茎尖的最佳消毒时间为3min;最佳诱导培养基为MS+6-BA5mg·L-1+NAA1mg·L-1;最佳分化培养基为MS+6-BA5mg·L-1+NAA0.1mg·L-1,最佳生根培养基为1/2MS+IBA0.25mg·L-1。 展开更多
关键词 五彩椒 抗寒性 组织培养
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辣椒EIL基因家族的鉴定及其在盐碱胁迫下的表达分析
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作者 吴鹏 李冬雪 郭茜茜 《河南农业科学》 北大核心 2024年第5期123-132,共10页
EIL(Ethylene-insensitive 3-like)基因在参与植物乙烯信号通路的转导和生长发育过程中发挥着重要作用。为了解辣椒EIL基因家族成员信息,利用生物信息学手段对辣椒EIL基因家族成员的理化性质、蛋白质结构、系统进化、基因结构、保守域... EIL(Ethylene-insensitive 3-like)基因在参与植物乙烯信号通路的转导和生长发育过程中发挥着重要作用。为了解辣椒EIL基因家族成员信息,利用生物信息学手段对辣椒EIL基因家族成员的理化性质、蛋白质结构、系统进化、基因结构、保守域、启动子顺式作用元件及CaEILs基因在不同组织部位和不同非生物胁迫条件下的表达模式进行分析。同时使用实时荧光定量PCR(qRT-PCR)对辣椒叶片中CaEILs在盐碱胁迫下的表达模式进行研究。结果表明,辣椒9个CaEILs分布在6条染色体上,氨基酸数目、蛋白质理论分子质量和脂肪系数分别介于209~677、23.77~76.07 ku和63.10~87.75,且主要为酸性、亲水的不稳定性核蛋白。系统进化分析显示,CaEILs被分成了4个亚家组,9个CaEILs在根、茎、叶、花蕾、花中具有不同程度的表达,而低温、高温、高盐、干旱胁迫下均能诱导CaEILs的表达,其对以上非生物胁迫均具有不同程度的响应。此外,利用qRT-PCR对盐碱胁迫下辣椒叶片中CaEILs进行了检测,结果发现,随着处理时间的延长,CaEIL1、CaEIL2、CaEIL4、CaEIL5、CaEIL8的表达量整体呈上升趋势,而CaEIL3、CaEIL6、CaEIL7、CaEIL9的表达量整体呈下降趋势。 展开更多
关键词 辣椒 EIl基因家族 基因结构 盐碱胁迫 表达
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气孔在辣椒(Capsicum annuum L.)子叶和下胚轴上的动态变化 被引量:1
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作者 董慧峰 王永飞 《种子》 CSCD 北大核心 2011年第1期49-52,共4页
以辣椒幼苗(Capsicum annuum L.)为材料,研究了辣椒生长发育过程中子叶的上、下表皮和下胚轴上、下端1 cm处的气孔密度及表皮细胞密度的动态变化。结果表明:(1)辣椒的气孔由肾形保卫细胞组成,无副卫细胞;子叶的表皮细胞呈无规则形,下胚... 以辣椒幼苗(Capsicum annuum L.)为材料,研究了辣椒生长发育过程中子叶的上、下表皮和下胚轴上、下端1 cm处的气孔密度及表皮细胞密度的动态变化。结果表明:(1)辣椒的气孔由肾形保卫细胞组成,无副卫细胞;子叶的表皮细胞呈无规则形,下胚轴的表皮细胞呈长方形或椭圆形;(2)辣椒子叶上、下表皮的气孔密度和表皮细胞密度分别在辣椒种子出芽后第6天和第3天达到最大,之后随子叶的生长逐渐减小,下表皮的气孔密度和表皮细胞密度明显大于上表皮的;(3)辣椒下胚轴上、下端1 cm处的气孔密度随下胚轴的生长变化不明显,但是表皮细胞密度随下胚轴的生长逐渐减小,下胚轴上端的气孔密度和表皮细胞密度明显的大于下胚轴下端的。 展开更多
关键词 辣椒(capsicum annuum l.) 气孔密度表皮细胞密度 下胚轴 子叶
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Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L. 被引量:4
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作者 CAO Ya-cong ZHANG Zheng-hai +3 位作者 WANG Li-hao SUI Xiao-lei ZHANG Zhen-xian ZHANG Bao-xi 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第4期775-784,共10页
Fruit set and development are affected by many phytohormones, including gibberellin. Little is known regarding molecular mechanism underlying gibberellin mediated fruit set and development especially in Capsicum. Thre... Fruit set and development are affected by many phytohormones, including gibberellin. Little is known regarding molecular mechanism underlying gibberellin mediated fruit set and development especially in Capsicum. Three gibberellin recep- tors, CaGIDlb.1, CaGIDlb.2 and CaGIDlc, and a DELLA protein, CaGAI, have been identified in Capsicum annuum L. During the fruit development, the expression level of CaGIDlc was low, and the expression fold change is mild. However, CaGIDlb. 1 and CaGIDlb.2 were relatively higher and more acute, which indicates that CaGIDlb. 1 and CaGID1b.2 may play an important role in fruit pericarp, placenta and seed. Ectopic expressions of CaGIDlb. 1, CaGIDlb.2 and CaGIDlc in Arabidopsis double mutant gidla gidlc increased plant height, among which CaGIDlb.2 had the most significant effect; CaGAI reduced plant height in double mutant rga-24/gai-t6, having a similar function to AtGID1 and AtGAI in stem elon- gation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that CaGIDlb.1 and CaGID1 b.2 interact with CaGAI in a GA-dependent manner, while CaGIDlc interacts with CaGAI in a GA-independent manner. Our study reveals the key elements during gibberellin signaling in Capsicum and supports the critical importance of gibberellin for Capsicum fruit set and development. 展开更多
关键词 capsicum annuum l. FRUIT GID1 DEllA
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Combining Ability Analyses of Net Photosynthesis Rate in Pepper (Capsicum annuum L.) 被引量:4
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作者 ZOU Xue-xiao MA Yan-qing +5 位作者 LIU Rong-yun ZHANG Zhu-qing CHENG Wen-chao DAI Xiong-ze LI Xue-feng ZHOU Qun-chu 《Agricultural Sciences in China》 CAS CSCD 2007年第2期159-166,共8页
In perspective of breeding high-yield hybrid pepper varieties, combining ability analysis of net photosynthesis rate at different phases of flowering and fruit setting in pepper was made with 15 cross combinations fro... In perspective of breeding high-yield hybrid pepper varieties, combining ability analysis of net photosynthesis rate at different phases of flowering and fruit setting in pepper was made with 15 cross combinations from 6 parents by (1/2) n (n- 1) diallel crosses. There are relatively large differences not only in general combining ability (GCA) effect among different parents and at different phases of flowering and fruit setting, but also in specific combining ability (SCA) effect among different hybrids. There are relatively large GCA effects in late parents but relatively less GCA effects in early parents. No obvious laws have been found in the relationship between SCA effects and maturity of hybrids. Variances of SCA are larger than those of GCA. Heritability is less but influence of environment is larger. Correlation analysis of combining ability between net photosynthesis rate and agronomic character or resistances to main diseases has showed that correlation coefficients of GCA are relatively large at the medium phase and the late phase of flowering and fruit setting. Net photosynthesis rate is more relative to leaf characters and fruit characters. Correlation coefficients of SCA are relatively large at the early phase and the late phase of flowering and fruit setting. Net photosynthesis rate is more relative to leaf characters and plant characters at the early phase but to plant characters and fruit characters at the late phase. Correlation coefficients of SCA between net photosynthesis rate and resistances to main diseases are larger than those of GCA. The combining abilities of net photosynthesis rate at different phases of flowering and fruit setting are positively correlated with those of yield per plant. The combining ability is an important parameter of breeding of high photosynthesis hybrid pepper varieties. 展开更多
关键词 pepper capsicum annuum l.) net photosynthesis rate combining ability STABIlITY correlation analysis
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Effect of continuous negative pressure water supply on the growth, development and physiological mechanism of Capsicum annuum L. 被引量:8
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作者 LI Di LONG Huai-yu +3 位作者 ZHANG Shu-xiang WU Xue-ping SHAO Hong-ying WANG Peng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第9期1978-1989,共12页
Effects of continuous negative pressure water supply on water consumption, growth and development, as well as physiological mechanism and quality of Capsicum annuum L. were investigated in this paper. Meanwhile, the o... Effects of continuous negative pressure water supply on water consumption, growth and development, as well as physiological mechanism and quality of Capsicum annuum L. were investigated in this paper. Meanwhile, the optimal negative pressure water supply conditions for growth of C. annuum L. were screened out to achieve the goals of water conservation, high yield and high quality, thus providing theoretical foundation for its field production. The pot experiment within the greenhouse was utilized; the continuous negative pressure water supply was adopted; the four treatments, artificial watering(CK), –5 k Pa(T1), –10 k Pa(T2), and –15 k Pa(T3) were set; and the daily water consumption, yield, as well as the biomass, nitrate reductase, root activity, vitamin C, capsaicin, and nutrient uptakes of nitrogen(N), phosphorus(P) and potassium(K) during various stages of its growth were determined. Compared with CK, when the water supply pressure was controlled at –5 to –15 k Pa in the experiment, the total water consumption of C. annuum L. reduced by 53.42 to 67.75%, the total water consumption intensity reduced by 54.29 to 67.14%, and the water use efficiency increased by 12.66 to 124.67%. The N accumulation in a single strain of C. annuum L. from the color turning stage to the red ripe stage increased by 15.99 to 100.55%, respectively, compared with that of CK; the P accumulation increased by 20.47 to 154.00% relative to that of CK, and the K accumulation increased by 64.92 to 144.9% compared with that of CK. Compared with CK, C. annuum L. yield was remarkably improved by 13.79% at T1, and contents of vitamin C, capsaicin as well as carotenoids at all growth stages were enhanced by 13.42–147.01%, 11.54–71.01%, and 41.1–568.06%, respectively. Nitrate reductase activity, root activity and chlorophyll(a+b) were markedly increased by 335.78–500%, 79.6–140.68% and 114.95–676.19%, respectively, from immature stage to full ripe stage. Adopting the continuous negative pressure water supply for C. annuum L. has a significant water-saving effect, and the water supply pressure being stable at –5 k Pa contributes to its growth and development, improves yield, enhances root activity, promotes nutrient uptake, and improves its quality, thus achieving the effects of water conservation, high yield, high quality and high efficiency. 展开更多
关键词 negative pressure water supply capsicum annuum l. physiological indexes yield quality
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AFLP Analysis of Mitochondrial DNA from a Cytoplasmic Male Sterility Line and its Maintainer in Pepper (Capsicum annuum L.)
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作者 Z.M. Wu  K.L. Hu  D.Y. Wang 《分子植物育种》 CAS CSCD 2007年第2期261-262,共2页
Cytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability of a plant to produce functional pollen, which is widespread among higher plants. CMS system is a valuable tool for plant
关键词 线立体 细胞质 胡椒 育种技术
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Purification and Biochemical Characterization of a Protease Inhibitor Ⅱ Family from Jalapeno Pepper(Capsicum annuum L.)
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作者 Juan Pablo Carrillo-Montes Roberto Arreguin-Espinosa +1 位作者 Jose Luis Munoz-Sanchez Manuel Soriano-Garcia 《Advances in Bioscience and Biotechnology》 2014年第7期661-668,共8页
Capsicum annuum L. was initially domesticated in Mexico and northern Central America, and represented an ancient Neotropical plant food complex. The purpose of this paper is to report the isolation and purification of... Capsicum annuum L. was initially domesticated in Mexico and northern Central America, and represented an ancient Neotropical plant food complex. The purpose of this paper is to report the isolation and purification of a novo-member of a protease inhibitor from jalape&ntildeo pepper (Capsicum annuum L.) (PIJP). The molecular weight of PIJP inhibitor is 5.95 kDa with 56 amino acids and 6 Cys residues with high inhibitory activity to trypsin with a Ki value of 95 nM. This inhibitor according to the alignment with homologous from NCBI and Pfam databases is a member of proteinase inhibitors II. It is worthwhile to mention a major compositional difference between the proteinase inhibitor II families which have 8 Cys residues. PIJP is the first purified proteinase inhibitor, member of this family with only 6 Cys residues. 展开更多
关键词 Protease Inhibitory Activity Protease Inhibitor Protein Purification Jalapeno Pepper capsicum annuum l.
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Processing of Snack of Goat Chub Chili(Capsicum annuum L.)with Formulations of Tomato and Tomato-Marjoram
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作者 Nelson Loyola López Francisca Ibarra Rubio +1 位作者 Carlos Acuna Carrasco Mariela Arriola Herrera 《Agricultural Sciences》 2019年第4期476-486,共11页
The cultivation of goat chili has potentially increased in Chile, due to different varieties of gourmet products made with it;for this reason, new industrial projects are sought to the growing volume to adapt them to ... The cultivation of goat chili has potentially increased in Chile, due to different varieties of gourmet products made with it;for this reason, new industrial projects are sought to the growing volume to adapt them to the new trends in consumption habits, mainly focused on achieving an attractive meal, a fast preparation and consumption, while nutrients are provided to offer some benefits for human health. The “snacks” products that include the use of fruit satisfactorily respond to these requirements, in addition to providing marketing alternatives to the surplus of fruit exports. For this reason, the present test, consisting of the processing of snacks based on goat chub chili with the addition of tomato and marjoram, was carried out to increase the palatability and the sensorial quality of the final product. The following formulations were used: only chili (T1);chili and tomato (T2);chili, tomato and marjoram (T3). The latter one was characterized by a good flavor and aroma. A large amount of the fruit water content had been removed by drying it with forced hot air. A product without chemical preserves was obtained, with physical and chemical parameters similar to those required by similar foods existing on the market today. In sensorial terms, the formulation to which marjoram was added showed good sensorial and acceptability attributes, highlighting them from the rest of the treatments. The snack color was maintained after the dehydration process, according to panelists. 展开更多
关键词 Dehydrated SNACK capsicum annuum l. Goat Chub
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Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode 被引量:2
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作者 Yang Liu Hongyi Cao +5 位作者 Jian Ling Yuhong Yang Yan Li Bingyan Xie Jianlong Zhao Zhenchuan Mao 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第1期133-144,共12页
Root-knot nematodes(RKNs)cause severe diseases in peppers annually around the world.In pepper,the Me3 gene provides a heat-stable and broad-spectrum resistance to RKNs.In this study,several simple sequence repeat(SSR)... Root-knot nematodes(RKNs)cause severe diseases in peppers annually around the world.In pepper,the Me3 gene provides a heat-stable and broad-spectrum resistance to RKNs.In this study,several simple sequence repeat(SSR)markers and insertion/deletion(In Del)markers were developed to fine map the Me3 gene.Analysis of 2272 individuals(F2progenies)revealed that Me3 was located in a 45-kb DNA region between markers SSR784 and SSR339,in which there were three candidate genes.Among them,as a novel nucleotide binding site and leucine rich repeat(NBS-LRR)family gene,the DNA sequence of Capana09g000163 of pepper line‘HDA149’was 6348 bp in length,with a 2802-bp open reading frame encoding 933 amino acids,including NB-ARC and LRR domains.Tobacco transient expression assays demonstrated that expression of Capana09g000163 triggered a hypersensitive response(HR)in Nicotiana benthamiana leaves.Subcellular localization results showed that the Capana09g000163 protein was localized in the cell nucleus.Ectopic expression of Capana09g000163 in Arabidopsis significantly increased resistance against Meloidogyne incognita compared with the wild-type(WT)Arabidopsis.Furthermore,M.incognita was almost unable to develop in transgenic Arabidopsis expressing Capana09g000163.Taken together,we cloned the Me3 gene and verified that it induced resistance against M.incognita with the methods of map-based cloning and transgenic technology,which may be of great significance to pepper breeding for resistance against RKNs. 展开更多
关键词 capsicum annuum l Meloidogyne incognita Nematode-resistant gene Fine mapping Transgenic technology
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辣椒抗病分子育种研究进展
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作者 雷建军 陈长明 +7 位作者 朱张生 郑婕 吴昊 肖艳辉 蒋园园 原远 陈国菊 曹必好 《广东农业科学》 CAS 2024年第4期1-18,共18页
辣椒起源于南美洲,16世纪传入中国。辣椒的营养价值高,在保健、美容、食品、医药、制暴等行业均有重要作用,已成为我国最重要的蔬菜作物之一,栽培面积逐渐增加、目前位居我国蔬菜作物之首。辣椒栽培过程中会遭受各种病害的侵袭,给生产... 辣椒起源于南美洲,16世纪传入中国。辣椒的营养价值高,在保健、美容、食品、医药、制暴等行业均有重要作用,已成为我国最重要的蔬菜作物之一,栽培面积逐渐增加、目前位居我国蔬菜作物之首。辣椒栽培过程中会遭受各种病害的侵袭,给生产造成重大损失。通过化学防治方法、农业防治措施等可以在一定程度上减轻病虫为害,但选用抗病品种是防治病害最经济有效的措施。随着分子生物学的发展,抗病分子育种与常规育种方法相结合是将来取得突破的发展方向。近年来,辣椒抗病分子育种机理取得了重要成果,尤其是抗病分子机理取得一系列成果。分子育种主要包括两部分:一是分子标记辅助育种,首先必须找到分子标记,分子标记分为基因标记、构建分离群体进行连锁分析获得的标记以及通过大量材料测序进行关联分析获得的标记;二是基因工程育种,前提是分离获得抗病基因,然后将抗病基因导入到经济性状优良的育种材料中,或者通过基因编辑技术或基因沉默技术使负向调控的基因发生突变,从而提高植株抗病性。该文从辣椒抗病性分子标记及其辅助选择、辣椒抗病分子机理、采用基因工程技术获得抗病辣椒种质(抗病毒病、抗青枯病和抗疫病)等方面进行综合介绍,并对辣椒抗病分子育种的发展趋势和研究重点进行展望。 展开更多
关键词 辣椒 分子标记 辅助选择 抗病分子机理 转基因
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辣椒种质资源PMMoV抗性基因检测与抗性鉴定
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作者 陈建分 曹振木 +6 位作者 秦于玲 申龙斌 刘维侠 朱丹 吴怡婷 刘子记 王旭 《热带作物学报》 CSCD 北大核心 2024年第4期682-690,共9页
辣椒轻斑驳病毒(Pepper mild mottle virus,PMMoV)是世界范围内最严重的病原体之一,目前已报道的PMMoV包括P_(0)、P_(1)、P_(1,2)、P_(1,2,3)、P_(1,2,3,4)五种致病型。辣椒轻斑驳病毒寄主范围广、致病力强,严重影响辣椒的产量和品质,... 辣椒轻斑驳病毒(Pepper mild mottle virus,PMMoV)是世界范围内最严重的病原体之一,目前已报道的PMMoV包括P_(0)、P_(1)、P_(1,2)、P_(1,2,3)、P_(1,2,3,4)五种致病型。辣椒轻斑驳病毒寄主范围广、致病力强,严重影响辣椒的产量和品质,开展抗病育种是防治该病毒最为经济有效的手段。本课题组前期研究已明确了感染海南儋州辣椒种质资源圃的PMMoV为P_(1,2)致病型,为了筛选抗PMMoV的辣椒种质资源,以110份种质为材料,利用已报道的与L^(3)连锁的分子标记对辣椒种质进行检测。结果表明:在110份材料中共检测到46份辣椒种质含有与L^(3)连锁的分子标记。采用苗期人工接种鉴定方法,对含有抗性分子标记的46份辣椒种质接种PMMoV P_(1,2)致病型进行抗性鉴定。接种5 d后调查局部症状,接种20 d后调查系统症状,计算病情指数。结果表明:不同的辣椒种质在抗病性上存在显著差异,各种质的病情指数在8.86~60.00之间,对PMMoV P_(1,2)致病型表现高抗(HR)的种质为CF19-34m和19FB6-1;表现抗病(R)的种质为CCJ93-1;表现中抗(MR)的种质包括17YB32、17SCa28m、15SM39-2、14SM555×14SM1、14SM567-2、14SM526-1、14SM565-1、14SM502-1、14SM519-1、14SM504m、14SM503m、14SM519-3、14SM518-2、14SM519-2、CCJ52-1等34份;表现感病(S)的种质包括14SM514m、CCJ22-1、CCJ113-2、CCJ135-1、CCJ157-1、L537-1m、L545、CCJ87-2、CCJ133-1,获得的高抗、抗病、中抗种质占总鉴定材料的80.43%。本研究结果为创制抗辣椒轻斑驳病毒种质资源和培育抗性品种奠定基础。 展开更多
关键词 辣椒 种质资源 PMMoV 分子标记 抗病性鉴定
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辣椒分子遗传图谱的构建和胞质雄性不育恢复性的QTL分析 被引量:51
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作者 张宝玺 王立浩 +3 位作者 黄三文 郭家珍 杨桂梅 堵玫珍 《中国农业科学》 CAS CSCD 北大核心 2003年第7期818-822,共5页
以辣椒胞质雄性不育的保持系Yolowonder、恢复系Perennial及其F1构建的DH群体与不育系 770 13A测交的群体为供试材料 ,用AFLP分子标记技术构建了包括 43个标记 8个连锁群的辣椒分子遗传图谱。在对测交群体育性调查的基础上 ,把控制辣椒... 以辣椒胞质雄性不育的保持系Yolowonder、恢复系Perennial及其F1构建的DH群体与不育系 770 13A测交的群体为供试材料 ,用AFLP分子标记技术构建了包括 43个标记 8个连锁群的辣椒分子遗传图谱。在对测交群体育性调查的基础上 ,把控制辣椒胞质雄性不育恢复性的QTL初步定位到第 1、3、6、8个连锁群上。 展开更多
关键词 辣椒 分子遗传图谱 图谱构建 胞质 雄性不育恢复性 QTl分析
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275份辣椒种质资源表型性状的遗传多样性分析
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作者 袁娟伟 贾利 +8 位作者 唐菁 翟永琪 汪胜 严从生 方凌 张其安 俞飞飞 甘德芳 江海坤 《安徽农业大学学报》 CAS CSCD 2024年第2期244-255,共12页
为了挖掘能够应用于遗传改良的种质资源,并筛选出优良表现的核心种质资源,为辣椒品种改良创新提供理论依据,以275份辣椒为研究材料,对42个表型性状进行遗传多样性分析、相关性分析、主成分分析以及聚类分析。结果表明:15个数量性状的Sha... 为了挖掘能够应用于遗传改良的种质资源,并筛选出优良表现的核心种质资源,为辣椒品种改良创新提供理论依据,以275份辣椒为研究材料,对42个表型性状进行遗传多样性分析、相关性分析、主成分分析以及聚类分析。结果表明:15个数量性状的Shannon-Wiener多样性指数(H')的变化范围为1.90~2.26,其中叶片长的H'值最高为2.26,其变异系数(coefficient of variation,CV)的变化范围为22.38%~146.09%。以辣椒素含量的变异系数最高为146.09%,变异幅度为48~290231SHU。27个质量性状的Shannon-Wiener多样性指数(H')的变化范围为0.03~2.81,其中果形有13种分布类型,其频率分布范围为1.87%~31.84%,大多数为灯笼形与羊角形,其遗传多样性指数也是最高为2.81,表明275份辣椒种质的表型性状具有丰富的遗传多样性。相关性分析结果表明,各表型性状间的相关系数中达到显著或极显著的有189对性状,这表明许多表型性状间相互影响。主成分分析结果表明,前13个主成分累加贡献率为68.62%,代表了辣椒表型性状的主要信息量,说明这13个主成分可反映42个表型性状的基本特征,从特征值和贡献率来看,果横径、单果重、果形、果肩形状、胎座大小,叶片长、叶片宽、叶柄长、株幅、分枝性、茎茸毛、叶面茸毛、花冠色、花药颜色、花柱颜色和果色共17个表型性状是引起辣椒种质表型不同的主要因素。聚类分析结果表明,在遗传距离为40.0时,275份辣椒种质可聚为5个类群。该研究为辣椒种质资源的利用、创新及品种选育提供了重要参考。 展开更多
关键词 辣椒 种质资源 表型分析 遗传多样性分析
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我国辣椒生产相关标准体系现状分析与展望
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作者 彭月 马艳青 +1 位作者 高风云 郑井元 《中国蔬菜》 北大核心 2024年第3期7-18,共12页
辣椒全产业链标准体系的构建对于打造“三品一标”辣椒产品、促进全产业链高质量发展具有重要意义。在系统总结我国与辣椒相关的标准体系情况的基础上,重点分析了辣椒高效生产、病害防治和加工安全等方面标准化体系建设的现状和不足,并... 辣椒全产业链标准体系的构建对于打造“三品一标”辣椒产品、促进全产业链高质量发展具有重要意义。在系统总结我国与辣椒相关的标准体系情况的基础上,重点分析了辣椒高效生产、病害防治和加工安全等方面标准化体系建设的现状和不足,并对今后在辣椒产品品质、绿色安全高效生产技术等方面的标准化体系建设进行了展望,以期为相关科学研究、技术推广、成果转化等提供借鉴与参考。 展开更多
关键词 辣椒 标准体系 生产安全 病害防治 加工安全
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利用分子标记对辣椒抗马铃薯Y病毒的3个QTLs进行选择 被引量:15
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作者 王立浩 张宝玺 +2 位作者 C.Caranta 毛胜利 A.Palloix 《园艺学报》 CAS CSCD 北大核心 2008年第1期53-58,共6页
为获得含有特定基因型的个体,深入研究辣椒抗马铃薯Y病毒(PVY)不同QTLs之间的关系,利用AFLP、SCAR和CAPS标记在辣椒材料‘Perennial’和‘Yolo Wonder’组成的BC_2和BC_1S_1分离世代对抗PVY的3个QTLs位点进行了选择。结果从BC_2群体中选... 为获得含有特定基因型的个体,深入研究辣椒抗马铃薯Y病毒(PVY)不同QTLs之间的关系,利用AFLP、SCAR和CAPS标记在辣椒材料‘Perennial’和‘Yolo Wonder’组成的BC_2和BC_1S_1分离世代对抗PVY的3个QTLs位点进行了选择。结果从BC_2群体中选出47个在主效QTL上含有抗性位点的单株,从中选出25个在LG9 PY2染色体上含有抗性位点的单株,最后选出11个在LG11 P10染色体上含有抗性位点的单株;从BC_1S_1群体中选出55个在LG9 PY2染色体上具有纯合抗性位点的单株,从中选出12个在主效QTL上纯合抗性位点的单株。经卡平方检测,实际分离比例同理论分离比例差异不显著。 展开更多
关键词 辣椒 马铃薯Y病毒 分子标记辅助选择 QTlS
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辣椒细胞质雄性不育基因的AFLP分析 被引量:10
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作者 刘科伟 王述彬 +3 位作者 刘金兵 潘宝贵 万红建 陈劲枫 《分子植物育种》 CAS CSCD 2009年第4期736-742,共7页
本文采用AFLP(amplified fragment length polymorphism)技术对辣椒(Capsicum annuum L.)细胞质雄性不育系21A及其相应的保持系21B基因组DNA进行多态性比较,筛选了64对AFLP选择性引物PstⅠ-NNN+MseⅠ-NNN组合,有60对引物组合均在两系间... 本文采用AFLP(amplified fragment length polymorphism)技术对辣椒(Capsicum annuum L.)细胞质雄性不育系21A及其相应的保持系21B基因组DNA进行多态性比较,筛选了64对AFLP选择性引物PstⅠ-NNN+MseⅠ-NNN组合,有60对引物组合均在两系间扩增出5447条带,多态性位点为4个,其中在不育系材料中仅有2个多态性片段AGC/CGC378和ATC/GAG446,对特异片段进行克隆、测序,其序列全长分别为340bp和408bp,它们编码的氨基酸序列与烟草基因组线粒体DNA序列同源性达94%和96%。 展开更多
关键词 辣椒 细胞质雄性不育(CMS) 扩增片段长度多态性(AFlP) 序列分析
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辣椒雄性不育的分子研究进展
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作者 张怡文 徐兰婷 +3 位作者 王飞 刘奕清 姚明华 徐凯 《中国瓜菜》 CAS 北大核心 2024年第2期1-7,共7页
辣椒(Capsicum annuum L.)是世界上重要的蔬菜作物之一,杂种优势明显。利用雄性不育系制种可有效解决人工去雄的难题,简化制种工序,降低生产成本。而辣椒雄性不育是一个快速发展的研究领域。综述了近几年在辣椒雄性不育的类型与特点、... 辣椒(Capsicum annuum L.)是世界上重要的蔬菜作物之一,杂种优势明显。利用雄性不育系制种可有效解决人工去雄的难题,简化制种工序,降低生产成本。而辣椒雄性不育是一个快速发展的研究领域。综述了近几年在辣椒雄性不育的类型与特点、细胞学特征、细胞核雄性不育的分子机制,以及细胞质雄性不育的机制解析等方面所取得的重要进展,并对辣椒雄性不育中存在的问题以及未来发展方向进行了讨论和展望,旨在为辣椒三系配套制种提供理论参考。 展开更多
关键词 辣椒 细胞核雄性不育 细胞质雄性不育 细胞学特征
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外源褪黑素对辣椒果实采后生理特性及贮藏品质的影响
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作者 刘微 唐帅 +4 位作者 李佳薇 王娜 曹丽敏 易诚 刘宇华 《湖南生态科学学报》 CAS 2024年第1期49-57,共9页
【目的】为探究外源褪黑素对辣椒果实采后生理特性及贮藏品质的影响。【方法】利用不同浓度(0、50、100、150、200和250μmol/L)褪黑素对辣椒果实进行浸泡处理2h,确定辣椒果实采后保鲜的最佳褪黑素浓度,并分析最佳褪黑素浓度处理后,贮... 【目的】为探究外源褪黑素对辣椒果实采后生理特性及贮藏品质的影响。【方法】利用不同浓度(0、50、100、150、200和250μmol/L)褪黑素对辣椒果实进行浸泡处理2h,确定辣椒果实采后保鲜的最佳褪黑素浓度,并分析最佳褪黑素浓度处理后,贮藏过程中辣椒果实色泽、衰老、营养品质及抗氧化酶活性等的变化。【结果】适宜浓度(100~200μmol/L)褪黑素处理能降低果实(贮藏第12天)的失重率和丙二醛含量,维持果实硬度、亮度及风味品质,当褪黑素浓度为150μmol/L时,辣椒果实的色泽、硬度、可溶性固形物含量、可溶性蛋白质含量均为最大值,相比对照组分别提高了8.70%(以L*表示)、27.73%、23.22%、24.64%,然而丙二醛的含量相比对照组下降了32.48%。进一步的动态分析发现贮藏早期150μmol/L(最佳浓度)外源褪黑素对辣椒果实生理品质无显著影响,但贮藏第8天后,外源褪黑素可显著提高辣椒果实中过氧化氢酶和过氧化物酶的活性,降低果实的失重率、丙二醛含量及可溶性果胶含量,维持果实亮度及硬度。此外,外源褪黑素处理还可改善贮藏过程中辣椒果实的风味品质。贮藏第8天后,外源褪黑素组辣椒果实中维生素C、可溶性蛋白质、可溶性固形物、总酚的含量均高于对照组,增长幅度分别在11.71%~21.0%、5.55%~12.93%、15.21%~25.32%、11.05%~24.03%。【结论】外源褪黑素可诱导辣椒果实抗氧化酶活性的增强,降低丙二醛及可溶性果胶的积累,进而抑制果实衰老,维持或改善贮藏过程中辣椒果实的品质。 展开更多
关键词 辣椒(capsicumannuum l.) 果实品质 褪黑素 保鲜
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