[Objective] The paper was to explore the effect of different culture conditions on sporulation quantity of Phytophthora capsici in Capsicum annuum L.var.dactylus M.in Xunhua.[Method] The effects of light hours,pH,medi...[Objective] The paper was to explore the effect of different culture conditions on sporulation quantity of Phytophthora capsici in Capsicum annuum L.var.dactylus M.in Xunhua.[Method] The effects of light hours,pH,medium and temperature on sporulation quantity during isolation and culture process of P.capsici were studied.[Result] The sporulation quantity of P.capsici under the conditions of 24 h/day light,pH 7.0,potato medium（PSA） and 30 ℃ was the largest,and pH,basal medium and temperature had greater impact on sporulation quantity.[Conclusion]The study laid foundation for the study on natural incidence condition of C.annuum in Xunhua.展开更多
为了获得甜椒雄性不育基因的分子标记,提高甜椒雄性不育性状选择的准确性和科学性。本研究以甜椒隐性细胞核雄性不育两用系AB91为试材,通过BSA法构建不育、可育基因池,利用全基因组重测序技术,筛选出甜椒隐性细胞核雄性不育候选基因27个...为了获得甜椒雄性不育基因的分子标记,提高甜椒雄性不育性状选择的准确性和科学性。本研究以甜椒隐性细胞核雄性不育两用系AB91为试材,通过BSA法构建不育、可育基因池,利用全基因组重测序技术,筛选出甜椒隐性细胞核雄性不育候选基因27个;并根据候选基因设计SSR分子标记8对,结果从8对SSR引物中获得2对具有多态性标记的引物。利用已筛选的2对SSR分子标记对251个F2群体进行检测和遗传距离测定,结果表明,AB91核不育基因与甜椒核雄性不育基因连锁的位点标记为H12、H24,与不育基因msms的连锁距离分别为0.29 c M、0.00 c M,缩短了与甜椒核不育ms基因的遗传距离,为进一步克隆甜椒核雄性不育基因提供了理论依据。展开更多
文摘[Objective] The paper was to explore the effect of different culture conditions on sporulation quantity of Phytophthora capsici in Capsicum annuum L.var.dactylus M.in Xunhua.[Method] The effects of light hours,pH,medium and temperature on sporulation quantity during isolation and culture process of P.capsici were studied.[Result] The sporulation quantity of P.capsici under the conditions of 24 h/day light,pH 7.0,potato medium（PSA） and 30 ℃ was the largest,and pH,basal medium and temperature had greater impact on sporulation quantity.[Conclusion]The study laid foundation for the study on natural incidence condition of C.annuum in Xunhua.
文摘为建立甜椒(Capsicum annuum var.grossum)花药培养及再生植株技术体系,对影响花药胚状体诱导和分化的因素进行了研究。结果表明,培养基组成对胚状体诱导率的影响以NAA>基本培养基>椰乳>KT,最佳胚状体诱导培养基为NTH+0.1 mg L–1 NAA+10%椰乳+1 mg L–1 KT+50μmol L–1 Ag NO3+30 g L–1蔗糖+5 g L–1琼脂+2 g L–1活性炭。花药经过24 h低温预处理和8 d高温预培养后,胚状体诱导率可达23.38%。植物生长调节剂对胚状体出芽率的影响为6-BA>NAA>IAA,最佳胚状体分化培养基为NTH+1 mg L–1 6-BA+0.3 mg L–1 NAA+0.1 mg L–1 IAA+30 g L–1蔗糖+5 g L–1琼脂。胚芽转入1/2MS+0.5 mg L–1 IBA+30 g L–1蔗糖+5 g L–1琼脂培养基后,生根率可达92.5%。
文摘为了获得甜椒雄性不育基因的分子标记,提高甜椒雄性不育性状选择的准确性和科学性。本研究以甜椒隐性细胞核雄性不育两用系AB91为试材,通过BSA法构建不育、可育基因池,利用全基因组重测序技术,筛选出甜椒隐性细胞核雄性不育候选基因27个;并根据候选基因设计SSR分子标记8对,结果从8对SSR引物中获得2对具有多态性标记的引物。利用已筛选的2对SSR分子标记对251个F2群体进行检测和遗传距离测定,结果表明,AB91核不育基因与甜椒核雄性不育基因连锁的位点标记为H12、H24,与不育基因msms的连锁距离分别为0.29 c M、0.00 c M,缩短了与甜椒核不育ms基因的遗传距离,为进一步克隆甜椒核雄性不育基因提供了理论依据。
