In recent years, grafting to improve salt-tolerance of vegetables has been studied extensively. The rootstock species, Solanum melongena cv Black Beauty, Solanum lycopersicum cv Akash and Solanum nigrum （bitter gumma...In recent years, grafting to improve salt-tolerance of vegetables has been studied extensively. The rootstock species, Solanum melongena cv Black Beauty, Solanum lycopersicum cv Akash and Solanum nigrum （bitter gumma）, reportedly salt-tolerant, were evaluated for their graft compatibility with Capsicum chinense cv Scotch Bonnet. Grafted and non-grafted plants were grown for three weeks under greenhouse conditions. Graft compatibility was determined by the regeneration of vascular bundles across the graft interface and vegetative growth parameters. The results showed that the highest survival percentage （100%） was obtained in the Scotch Bonnet/Black Beauty heterografts and the lowest （16%） was obtained in the Scotch Bonnet/bitter gumma heterografts. Vascular regeneration across the graft interface was seen in Scotch Bonnet autografts and heterografts with Black Beauty. Graft incompatibility observed in heterografts on Akash and bitter gumma rootstocks could be attributed to the presence of a necrotic layer at the graft interface. In conclusion, progressive scion growth and development and the regeneration of vascular bundles across the graft interface are effective in determining graft compatibility between Scotch Bonnet and selected solanceous species. This suggests that Scotch Bonnet can be cultivated under saline growing condition.展开更多
Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were...Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were analyzed to quantify their phenolic compounds, carotenoids and ascorbic acid contents, and to measure their free radical scavenging (ABTS assay) and antioxidant activities (β-carotene/linoleic acid assay). Phenolics (20.54 to 20.75 mg/100 g sample), carotenoids (1.00 to 1.26 mg/100 g sample) and ascorbic acid contents (187.24 to 281.73 mg/100 g sample) varied between genotypes. Trolox equivalent antioxidant capacity (TEAC) ranged from 1.55 to 3.23 mM/mg sample. During the 120-min decolorization trial, antioxidant capacity decreased over time in the studied genotypes. Values ranged from 36%to 57% β-carotene bleaching during the first 30 minutes. Fruit from all seven studied genotypes are good antioxidant sources and hold promise as natural ingredients in functional foods.展开更多
In order to discuss the role of MYB gene in capsaicine synthesis process, one CcMYB gene was cloned from Capsicum chinense Jacq. by RT-PCR. Its cDNA has a total length of 1 038 bp, and was speculated to code 345 amino...In order to discuss the role of MYB gene in capsaicine synthesis process, one CcMYB gene was cloned from Capsicum chinense Jacq. by RT-PCR. Its cDNA has a total length of 1 038 bp, and was speculated to code 345 amino acids, comprising an complete open reading frame. The isoelectric point is 8.57, and the molecular weight is 38.2 KD. The protein is a neutral hydrophobin without transmentbrane structure. There are two MYBDNA domains at the N terminal. The fluorescence quantitative PCR results showed that CcMYB gene was expressed in all the root, stem, leaf, flower, placenta and fruit tissue of pepper, and the expression level was the highest in fruit ; and CcMYB was expressed in fruit at the highest level at turning stage, and at the second highest level at expansion stage, which accords with the expression profile of punl gene in fruit development period. It is speculated that CcMYB gene plays an important role in the regulation of capsaicine synthesis in C. chinense fruit.展开更多
The present study, which aims to contribute to the valorization of Capsicum chinense, focuses on ethnobotanical investigations and evaluation of insecticidal activity on Sitophilus zeamaïs and Aphis craccivor...The present study, which aims to contribute to the valorization of Capsicum chinense, focuses on ethnobotanical investigations and evaluation of insecticidal activity on Sitophilus zeamaïs and Aphis craccivora, as well as the germination power of infected maize and peanut grains treated and not treated with Capsicum chinense leaf powder. To do this, the moisture content of corn and peanuts was determined by the techniques: drying in the sun and in the oven. The insecticidal effect of the powder was tested on maize and peanut kernels infected by the AGRAR (2013) method, followed by a test of the germination potency of these treated grains. The average moisture values of corn and groundnut kernels are 14.51% and 12.25% respectively. The results show, a higher insecticidal efficacy of Capsicum chinense leaf powder on Sitophilus zeamaïs and Aphis craccivora with doses (2, 4 and 8 g). The average germination rates of maize and peanut kernels infected and treated with this powder are: (16.5%;63%;99.5%) and (21.25%;96.5%;99.75%), respectively. This study could be a bio-insecticide alternative for the peasant community in the conservation of cereal and legume products.展开更多
Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives the...Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives them industrial importance.Soil is an important factor that affects pepper development,nutritional quality,and capsaicinoid content.However,the effect of soil type on fruit development and capsaicinoid metabolism has been little understood.This work aimed to compare the effect of soils with contrasting characteristics,black soil(BS)and red soil(RS),on the expression of genes related to the development of fruits,and capsaicinoid synthesis using a transcriptomic analysis of the habanero pepper fruits.Plants growing in RS had bigger fruits and higher expression of genes related to floral development,fruit abscission,and softening which suggests that RS stimulates fruit development from early stages until maturation stages.Fruits from plants growing in BS had enrichment in metabolic pathways related to growth,sugars,and photosynthesis.Besides,these fruits had higher capsaicinoid accumulation at 25 days post-anthesis,and higher expression of genes related to the branched-chain amino acids metabolism(ketol-acid reductisomerase KARI),pentose phosphate pathway and production of NADPH(glucose-6-phosphate-1-dehydrogenase G6PDH),and proteasome and vesicular traffic in cells(26S proteasome regulatory subunit T4 RPT4),which suggest that BS is better in the early stimulation of pathways related to the nutritional quality and capsaicinoid metabolism in the fruits.展开更多
The phenotypic variation and its distribution among species,morphotypes,and among and within populations was estimated in 71 populations pertaining to 15 morphotypes of three domesticated species of Capsicum from Mexi...The phenotypic variation and its distribution among species,morphotypes,and among and within populations was estimated in 71 populations pertaining to 15 morphotypes of three domesticated species of Capsicum from Mexico.Collections were made in the states of Sinaloa,Nayarit,Oaxaca,Tabasco,and Yucatán in two agroecosystems:Backyard-garden and monoculture.Fifteen phenotypic characteristics were analyzed through one-way variance analysis and multivariate analyses of principal components analysis(PCA)and hierarchical clustering using Ward’s method.The study was performed in a uniform greenhouse experiment.A high variation was found among and within populations in all the measured characteristics.Of the total variation,13.0%was distributed among species,27.9%among morphotypes,8.1%among populations,and 51.0%within populations.Because plants grew in a uniform environment,these results indicate that the differences observed among and within species,morphotypes,and populations have a genetic basis.Univariate and multivariate analyses clearly differentiated morphotypes,suggesting that the category of morphotypes must be used to nominate the infraspecific variation in the domesticated Capsicum.The principal components analysis identified a total of 15 principal components that contributed to explain the total variation.The first two components explained 59.64%of the total variation and seven components explained more than 90%of the total variation.Among the measured characteristics,number of seed per fruit,weight of the fruit,width of the fruit,length of the fruit,stem diameter,days to flowering,and height of the plant contributed to component 1 variation,whereas width of the leaf,length of the leaf,and number of locules,number of fruits and number of seeds per plant contributed to component 2.The hierarchical clustering separated the populations and the morphotypes in two large different groups.One group consisted of populations collected in monoculture conditions and the other group corresponded to population collected from backyard-garden conditions.The monoculture populations were characterized mainly by their longer,wider,and higher weight fruits,plants were of less height,had smaller stem diameters,and lower number of fruits than the populations collected from the backyard-garden conditions.The backyard-garden populations of the Capsicum annuum and Capsicum frutescens species,considered wild or semi-domesticated,constituted a non-differentiated phenotypic group that does not allow dividing them in different species.展开更多
文摘In recent years, grafting to improve salt-tolerance of vegetables has been studied extensively. The rootstock species, Solanum melongena cv Black Beauty, Solanum lycopersicum cv Akash and Solanum nigrum （bitter gumma）, reportedly salt-tolerant, were evaluated for their graft compatibility with Capsicum chinense cv Scotch Bonnet. Grafted and non-grafted plants were grown for three weeks under greenhouse conditions. Graft compatibility was determined by the regeneration of vascular bundles across the graft interface and vegetative growth parameters. The results showed that the highest survival percentage （100%） was obtained in the Scotch Bonnet/Black Beauty heterografts and the lowest （16%） was obtained in the Scotch Bonnet/bitter gumma heterografts. Vascular regeneration across the graft interface was seen in Scotch Bonnet autografts and heterografts with Black Beauty. Graft incompatibility observed in heterografts on Akash and bitter gumma rootstocks could be attributed to the presence of a necrotic layer at the graft interface. In conclusion, progressive scion growth and development and the regeneration of vascular bundles across the graft interface are effective in determining graft compatibility between Scotch Bonnet and selected solanceous species. This suggests that Scotch Bonnet can be cultivated under saline growing condition.
基金This research was supported by the Fundación PRO-DUCE AC-Yucatán,México(Project“Determinación de las propiedades funcionales del chile habanero”).
文摘Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were analyzed to quantify their phenolic compounds, carotenoids and ascorbic acid contents, and to measure their free radical scavenging (ABTS assay) and antioxidant activities (β-carotene/linoleic acid assay). Phenolics (20.54 to 20.75 mg/100 g sample), carotenoids (1.00 to 1.26 mg/100 g sample) and ascorbic acid contents (187.24 to 281.73 mg/100 g sample) varied between genotypes. Trolox equivalent antioxidant capacity (TEAC) ranged from 1.55 to 3.23 mM/mg sample. During the 120-min decolorization trial, antioxidant capacity decreased over time in the studied genotypes. Values ranged from 36%to 57% β-carotene bleaching during the first 30 minutes. Fruit from all seven studied genotypes are good antioxidant sources and hold promise as natural ingredients in functional foods.
基金Supported by College Students'Innovation Fund of Jilin University(2017A82372)
文摘In order to discuss the role of MYB gene in capsaicine synthesis process, one CcMYB gene was cloned from Capsicum chinense Jacq. by RT-PCR. Its cDNA has a total length of 1 038 bp, and was speculated to code 345 amino acids, comprising an complete open reading frame. The isoelectric point is 8.57, and the molecular weight is 38.2 KD. The protein is a neutral hydrophobin without transmentbrane structure. There are two MYBDNA domains at the N terminal. The fluorescence quantitative PCR results showed that CcMYB gene was expressed in all the root, stem, leaf, flower, placenta and fruit tissue of pepper, and the expression level was the highest in fruit ; and CcMYB was expressed in fruit at the highest level at turning stage, and at the second highest level at expansion stage, which accords with the expression profile of punl gene in fruit development period. It is speculated that CcMYB gene plays an important role in the regulation of capsaicine synthesis in C. chinense fruit.
文摘The present study, which aims to contribute to the valorization of Capsicum chinense, focuses on ethnobotanical investigations and evaluation of insecticidal activity on Sitophilus zeamaïs and Aphis craccivora, as well as the germination power of infected maize and peanut grains treated and not treated with Capsicum chinense leaf powder. To do this, the moisture content of corn and peanuts was determined by the techniques: drying in the sun and in the oven. The insecticidal effect of the powder was tested on maize and peanut kernels infected by the AGRAR (2013) method, followed by a test of the germination potency of these treated grains. The average moisture values of corn and groundnut kernels are 14.51% and 12.25% respectively. The results show, a higher insecticidal efficacy of Capsicum chinense leaf powder on Sitophilus zeamaïs and Aphis craccivora with doses (2, 4 and 8 g). The average germination rates of maize and peanut kernels infected and treated with this powder are: (16.5%;63%;99.5%) and (21.25%;96.5%;99.75%), respectively. This study could be a bio-insecticide alternative for the peasant community in the conservation of cereal and legume products.
文摘Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives them industrial importance.Soil is an important factor that affects pepper development,nutritional quality,and capsaicinoid content.However,the effect of soil type on fruit development and capsaicinoid metabolism has been little understood.This work aimed to compare the effect of soils with contrasting characteristics,black soil(BS)and red soil(RS),on the expression of genes related to the development of fruits,and capsaicinoid synthesis using a transcriptomic analysis of the habanero pepper fruits.Plants growing in RS had bigger fruits and higher expression of genes related to floral development,fruit abscission,and softening which suggests that RS stimulates fruit development from early stages until maturation stages.Fruits from plants growing in BS had enrichment in metabolic pathways related to growth,sugars,and photosynthesis.Besides,these fruits had higher capsaicinoid accumulation at 25 days post-anthesis,and higher expression of genes related to the branched-chain amino acids metabolism(ketol-acid reductisomerase KARI),pentose phosphate pathway and production of NADPH(glucose-6-phosphate-1-dehydrogenase G6PDH),and proteasome and vesicular traffic in cells(26S proteasome regulatory subunit T4 RPT4),which suggest that BS is better in the early stimulation of pathways related to the nutritional quality and capsaicinoid metabolism in the fruits.
基金This research was funded by the Universidad Autónoma de Sinaloa(Project PROFAPI 2015/123).
文摘The phenotypic variation and its distribution among species,morphotypes,and among and within populations was estimated in 71 populations pertaining to 15 morphotypes of three domesticated species of Capsicum from Mexico.Collections were made in the states of Sinaloa,Nayarit,Oaxaca,Tabasco,and Yucatán in two agroecosystems:Backyard-garden and monoculture.Fifteen phenotypic characteristics were analyzed through one-way variance analysis and multivariate analyses of principal components analysis(PCA)and hierarchical clustering using Ward’s method.The study was performed in a uniform greenhouse experiment.A high variation was found among and within populations in all the measured characteristics.Of the total variation,13.0%was distributed among species,27.9%among morphotypes,8.1%among populations,and 51.0%within populations.Because plants grew in a uniform environment,these results indicate that the differences observed among and within species,morphotypes,and populations have a genetic basis.Univariate and multivariate analyses clearly differentiated morphotypes,suggesting that the category of morphotypes must be used to nominate the infraspecific variation in the domesticated Capsicum.The principal components analysis identified a total of 15 principal components that contributed to explain the total variation.The first two components explained 59.64%of the total variation and seven components explained more than 90%of the total variation.Among the measured characteristics,number of seed per fruit,weight of the fruit,width of the fruit,length of the fruit,stem diameter,days to flowering,and height of the plant contributed to component 1 variation,whereas width of the leaf,length of the leaf,and number of locules,number of fruits and number of seeds per plant contributed to component 2.The hierarchical clustering separated the populations and the morphotypes in two large different groups.One group consisted of populations collected in monoculture conditions and the other group corresponded to population collected from backyard-garden conditions.The monoculture populations were characterized mainly by their longer,wider,and higher weight fruits,plants were of less height,had smaller stem diameters,and lower number of fruits than the populations collected from the backyard-garden conditions.The backyard-garden populations of the Capsicum annuum and Capsicum frutescens species,considered wild or semi-domesticated,constituted a non-differentiated phenotypic group that does not allow dividing them in different species.
