<abstract>Aim: To manage male infertility with obstructive azoospermia by means of percutaneous epididymal sperm aspiration (PESA) and intrauterine insemination (IUI). Methods: Ninety azoospermic patients with c...<abstract>Aim: To manage male infertility with obstructive azoospermia by means of percutaneous epididymal sperm aspiration (PESA) and intrauterine insemination (IUI). Methods: Ninety azoospermic patients with congenital bilateral absence of the vas deferens (BAVD, n=58) or bilateral caudal epididymal obstruction (BCEO, n=32) requesting for fine needle aspiration (FNA), PESA and IUI were recruited. The obstruction was diagnosed by vasography and determination of the fructose, carnitine and alpha-glucosidase levels in the seminal fluid. Results: The mean sperm motility, density, abnormal sperm and total sperm count of the caput epdidymis were 16 %±22 %, (12±31) ×106/mL, 55 %±36 % and (16±14)×106, respectively. In the 90 couples, a total of 74 PESA procedures and 66 cycles of IUI were performed. Three pregnancies resulted, including one twin pregnancy giving birth to two healthy boys, one single pregnancy with a healthy girl and another single pregnancy aborted at week 6 of conception. The pregnancy rate per IUI cycle was 4.5 %. Conclusion: The birth of normal, healthy infants by IUI using PESA indicates that the caput epididymal sperm possess fertilization capacity. The PESA-IUI programme is a practical and economical procedure for the management of patients with obstructive azoospermia.展开更多
Caput succedaneum is relatively common at birth but infrequently diagnosed in utero. We report the first case of a prenatal incarcerated caput succedaneum after cervical cerclage in a patient with premature rupture of...Caput succedaneum is relatively common at birth but infrequently diagnosed in utero. We report the first case of a prenatal incarcerated caput succedaneum after cervical cerclage in a patient with premature rupture of the membranes(PPROM). A 41-year-old woman was referred and admitted to our hospital due to PPROM at 19 wk of gestation. Aggressive therapy, including amnioinfusion, cervical cerclage, and administration of antibiotics and tocolysis, was initiated. At 24 wk of gestation, a thumb tip-sized and polyp-like mass, which was irreducible, was delineated with a vaginal examination, vaginal speculum, and transvaginal ultrasonography, leading to the diagnosis of incarcerated caput succedaneum. Under general anesthesia, the incarcerated caput succedaneum was repositioned with fingers after cutting the string to avoid necrosis, and then, placement of a Mc Donald cervical cerclage was undertaken again. At 26 wk of gestation, she delivered a 678 g girl through an emergency cesarean section performed due to profuse bleeding and prolonged decelerations. A slight bulge with hair was observed on the head by palpation at birth. Cephalic ultrasonography, X-ray, magnetic resonance imaging and electroencephalogram confirmed no abnormality. Although the baby needed oxygen(0.2 L/min) at the time of hospital discharge, she has grown favorably at three years of corrected age.展开更多
采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取...采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。展开更多
A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation...A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation measurement. This HPⅠconsists of Fuc,Glc and Gal in the molar ratios of 0.423∶1∶2.110. Its structural features were investigated by methylation and GC-MS analysis,periodate oxidation-Smith degradation,and partial acid-hydrolysis. In addition, enzymatic degradation experiments and GC analysis were applied. The following structure of the highly branched repeating unit was established: 6→)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→ 2 ↑ 1 2 ↑ 1 2 ↑ 1 Gal-(1→4)-Fuc Glc-(1→3)-Glc 2 Gal-(1→6)-Glc 3 The activities of HPⅠwere tested in mice. All the samples exhibited high effect of prevention and cure against chronic gastricism.展开更多
文摘<abstract>Aim: To manage male infertility with obstructive azoospermia by means of percutaneous epididymal sperm aspiration (PESA) and intrauterine insemination (IUI). Methods: Ninety azoospermic patients with congenital bilateral absence of the vas deferens (BAVD, n=58) or bilateral caudal epididymal obstruction (BCEO, n=32) requesting for fine needle aspiration (FNA), PESA and IUI were recruited. The obstruction was diagnosed by vasography and determination of the fructose, carnitine and alpha-glucosidase levels in the seminal fluid. Results: The mean sperm motility, density, abnormal sperm and total sperm count of the caput epdidymis were 16 %±22 %, (12±31) ×106/mL, 55 %±36 % and (16±14)×106, respectively. In the 90 couples, a total of 74 PESA procedures and 66 cycles of IUI were performed. Three pregnancies resulted, including one twin pregnancy giving birth to two healthy boys, one single pregnancy with a healthy girl and another single pregnancy aborted at week 6 of conception. The pregnancy rate per IUI cycle was 4.5 %. Conclusion: The birth of normal, healthy infants by IUI using PESA indicates that the caput epididymal sperm possess fertilization capacity. The PESA-IUI programme is a practical and economical procedure for the management of patients with obstructive azoospermia.
文摘Caput succedaneum is relatively common at birth but infrequently diagnosed in utero. We report the first case of a prenatal incarcerated caput succedaneum after cervical cerclage in a patient with premature rupture of the membranes(PPROM). A 41-year-old woman was referred and admitted to our hospital due to PPROM at 19 wk of gestation. Aggressive therapy, including amnioinfusion, cervical cerclage, and administration of antibiotics and tocolysis, was initiated. At 24 wk of gestation, a thumb tip-sized and polyp-like mass, which was irreducible, was delineated with a vaginal examination, vaginal speculum, and transvaginal ultrasonography, leading to the diagnosis of incarcerated caput succedaneum. Under general anesthesia, the incarcerated caput succedaneum was repositioned with fingers after cutting the string to avoid necrosis, and then, placement of a Mc Donald cervical cerclage was undertaken again. At 26 wk of gestation, she delivered a 678 g girl through an emergency cesarean section performed due to profuse bleeding and prolonged decelerations. A slight bulge with hair was observed on the head by palpation at birth. Cephalic ultrasonography, X-ray, magnetic resonance imaging and electroencephalogram confirmed no abnormality. Although the baby needed oxygen(0.2 L/min) at the time of hospital discharge, she has grown favorably at three years of corrected age.
文摘采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。
文摘A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation measurement. This HPⅠconsists of Fuc,Glc and Gal in the molar ratios of 0.423∶1∶2.110. Its structural features were investigated by methylation and GC-MS analysis,periodate oxidation-Smith degradation,and partial acid-hydrolysis. In addition, enzymatic degradation experiments and GC analysis were applied. The following structure of the highly branched repeating unit was established: 6→)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→ 2 ↑ 1 2 ↑ 1 2 ↑ 1 Gal-(1→4)-Fuc Glc-(1→3)-Glc 2 Gal-(1→6)-Glc 3 The activities of HPⅠwere tested in mice. All the samples exhibited high effect of prevention and cure against chronic gastricism.
