Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes(Li et al.,2022).They are a class of C40 tetraterpenoids consisting of eight isoprenoid units,and can be classified into caro...Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes(Li et al.,2022).They are a class of C40 tetraterpenoids consisting of eight isoprenoid units,and can be classified into carotenes and xanthophylls on the basis of their functional groups(Saini et al.,2015).Carotenes can be lin‐ear(phytoene,phytofluene,andζ-carotene)or branched(β-carotene andα-carotene).Xanthophylls compriseβ,β-xanthophylls(β-cryptoxanthin,zeaxanthin,violaxanthins,and neoxanthin)andβ,ε-xanthophylls(α-cryptoxanthin,α-carotene,and lutein).展开更多
目的研究外膜蛋白CarO的表达、序列突变与鲍曼不动杆菌对亚胺培南敏感性的关系。方法收集鲍曼不动杆菌8株,E-test鉴定亚胺培南敏感性;用脉冲场凝胶电泳(PFGE)鉴定菌株同源性;PCR法检验CarO基因,并进行序列分析;Real time PCR法检验CarO ...目的研究外膜蛋白CarO的表达、序列突变与鲍曼不动杆菌对亚胺培南敏感性的关系。方法收集鲍曼不动杆菌8株,E-test鉴定亚胺培南敏感性;用脉冲场凝胶电泳(PFGE)鉴定菌株同源性;PCR法检验CarO基因,并进行序列分析;Real time PCR法检验CarO mRNA的表达;SDS电泳法比较CarO蛋白的表达。结果本实验发现4株耐药菌株CarO序列同源性高;所有菌株中均存在CarO基因,但亚胺培南敏感及耐药菌株基因序列相似性仅87%,敏感菌株与耐药菌株均有CarO mRNA基因表达,且两者表达水平差异无统计学意义;SDS电泳也发现其基因序列不同者CarO蛋白分子量不同。结论 CarO基因序列改变可能与鲍曼不动杆菌对亚胺培南敏感性的变化有关。展开更多
To investigate the protective effects of β-carotene in rats against the development of chronic bronchitis induced by cigarette smoking Methods Forty-two Male Wistar rats were randomly divided into three study grou...To investigate the protective effects of β-carotene in rats against the development of chronic bronchitis induced by cigarette smoking Methods Forty-two Male Wistar rats were randomly divided into three study groups: ① control (n=15), animals underwent no treatment; ② cigarette smoking (n=15), animals developed chronic bronchitis through long-term cigarette smoking twice a day for 75 d; ③ β-carotene plus cigarette smoking animals (n=12) were given 1 ml or 15 mg/kg β-carotene orally every day just before cigarette smoking The levels of IL-6, IL-8, NO, superoxide dismutase (SOD) and lipoperoxide (LPO) in serum, bronchoalveolar lavage fluid (BALF) and lung tissue were measured and the pathological changes to lung tissue were analyzed using light microscopy Results Long-term cigarette smoking caused an obvious increase in the amount of IL-6, IL-8 and LPO and a sharp decrease in the levels of NO and SOD in smoking animals compared to controls β-carotene intake reversed all the changes induced by smoking and alleviated the pathological changes caused by chronic bronchitis Conclusions Quantitative oral intake of β-carotene had protective effects against chronic bronchitis induced by long-term cigarette smoking, which was associated with the increased production of NO, the clearance of some oxidative free radicals (OFR) and the alleviation of chronic inflammation展开更多
基金This work was supported by the National Key Research and Development Program of China(No.2021YFD1600802-02)。
文摘Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes(Li et al.,2022).They are a class of C40 tetraterpenoids consisting of eight isoprenoid units,and can be classified into carotenes and xanthophylls on the basis of their functional groups(Saini et al.,2015).Carotenes can be lin‐ear(phytoene,phytofluene,andζ-carotene)or branched(β-carotene andα-carotene).Xanthophylls compriseβ,β-xanthophylls(β-cryptoxanthin,zeaxanthin,violaxanthins,and neoxanthin)andβ,ε-xanthophylls(α-cryptoxanthin,α-carotene,and lutein).
文摘To investigate the protective effects of β-carotene in rats against the development of chronic bronchitis induced by cigarette smoking Methods Forty-two Male Wistar rats were randomly divided into three study groups: ① control (n=15), animals underwent no treatment; ② cigarette smoking (n=15), animals developed chronic bronchitis through long-term cigarette smoking twice a day for 75 d; ③ β-carotene plus cigarette smoking animals (n=12) were given 1 ml or 15 mg/kg β-carotene orally every day just before cigarette smoking The levels of IL-6, IL-8, NO, superoxide dismutase (SOD) and lipoperoxide (LPO) in serum, bronchoalveolar lavage fluid (BALF) and lung tissue were measured and the pathological changes to lung tissue were analyzed using light microscopy Results Long-term cigarette smoking caused an obvious increase in the amount of IL-6, IL-8 and LPO and a sharp decrease in the levels of NO and SOD in smoking animals compared to controls β-carotene intake reversed all the changes induced by smoking and alleviated the pathological changes caused by chronic bronchitis Conclusions Quantitative oral intake of β-carotene had protective effects against chronic bronchitis induced by long-term cigarette smoking, which was associated with the increased production of NO, the clearance of some oxidative free radicals (OFR) and the alleviation of chronic inflammation
