The roots of Caragana sinica (Buc′hoz) Rehd is a Chinese folk medicine used for the treatment of asthenia syndrome, vascular hypertension, leukorrhagia, arthrodynia, and as antiinflammatory agent From the ethanolic...The roots of Caragana sinica (Buc′hoz) Rehd is a Chinese folk medicine used for the treatment of asthenia syndrome, vascular hypertension, leukorrhagia, arthrodynia, and as antiinflammatory agent From the ethanolic extract of the roots of C sinica, five isoflavonoid compounds were isolated and identified as flemichapparin B, formononetin, pseudobaptigenin, 5 hydroxy 7,4 ′ dimethoxy isoflavone and 5 hydroxy 7 methoxy 3 ′,4 ′ methylenedioxy isoflavone展开更多
A new tetrastilbene named carasinol D （1） was isolated from the roots of Caragana sinica. Its structure was elucidated by spectroscopic analysis. Compound 1 was obtained from carasinol B （2） by an acid-catalyzed c...A new tetrastilbene named carasinol D （1） was isolated from the roots of Caragana sinica. Its structure was elucidated by spectroscopic analysis. Compound 1 was obtained from carasinol B （2） by an acid-catalyzed conversion.展开更多
In order to efficiently explore and use woody biomass,six lignin fractions were isolated from dewaxed Caragana sinica via successive extraction with organic solvents and alkaline solutions.The lignin structures were c...In order to efficiently explore and use woody biomass,six lignin fractions were isolated from dewaxed Caragana sinica via successive extraction with organic solvents and alkaline solutions.The lignin structures were characterized by Fourier transform infrared spectroscopy(FT-IR) and 1D and 2D Nuclear Magnetic Resonance(NMR).FT-IR spectra revealed that the "core" of the lignin structure did not significantly change during the treatment under the conditions given.The results of 1H and 13C NMR demonstrated that the lignin fraction L2,isolated with 70% ethanol containing 1% NaOH,was mainly composed of β-O-4 ether bonds together with G and S units and trace p-hydroxyphenyl unit.Based on the 2D HSQC NMR spectrum,the ethanol organosolv lignin fraction L1,extracted with 70% ethanol,presents a predominance of β-O-4′ aryl ether linkages(61% of total side chains),and a low abundance of condensed carbon-carbon linked structures(such as ββ′,β-1′,and β-5′) and a lower S/G ratio.Furthermore,a small percentage(ca.9%) of the linkage side chain was found to be acylated at the γ-carbon.展开更多
目的探讨金雀根和黄芪配伍对糖尿病肾病(DKD)大鼠的影响。方法将SD大鼠随机分为正常组、模型组、恩格列净组、金雀根组、黄芪组、配伍组,每组10只。采用单侧切除肾脏联合腹腔注射链脲佐菌素(STZ)复制DKD大鼠模型,造模成功后灌胃相应剂...目的探讨金雀根和黄芪配伍对糖尿病肾病(DKD)大鼠的影响。方法将SD大鼠随机分为正常组、模型组、恩格列净组、金雀根组、黄芪组、配伍组,每组10只。采用单侧切除肾脏联合腹腔注射链脲佐菌素(STZ)复制DKD大鼠模型,造模成功后灌胃相应剂量药物8周。第0、4、8周检测24 h尿微量白蛋白(24 h U-mALB);ELISA法检测Scr、BUN、CysC、MDA水平及SOD活性;荧光探针法检测肾组织ROS表达;HE、PAS、Masson、PASM-Masson染色观察肾组织病理结构改变;免疫组织化学法检测肾组织NOX4、Drp1、MFN2、P62表达;Western blot法检测肾组织PINK1、MFN2、Parkin、LC3-Ⅱ/Ⅰ、P62、p-Drp1蛋白表达。结果与模型组比较,各给药组大鼠24 h U-mALB、BUN、Scr、CysC水平均降低(P<0.01),肾组织病理性结构损伤改善;血清和组织MDA水平降低(P<0.01),SOD活性升高(P<0.01);肾组织PINK1、MFN2、Parkin、LC3-Ⅱ/Ⅰ蛋白表达升高(P<0.05,P<0.01),p-Drp1、P62蛋白表达降低(P<0.01),其中配伍组作用优于单用药组(P<0.05,P<0.01)。结论金雀根和黄芪配伍可能是通过调控PINK1/MFN2/Parkin通路改善线粒体动力学,激活线粒体自噬,抑制氧化应激,减轻肾脏病理损伤,改善DKD大鼠肾功能。展开更多
文摘The roots of Caragana sinica (Buc′hoz) Rehd is a Chinese folk medicine used for the treatment of asthenia syndrome, vascular hypertension, leukorrhagia, arthrodynia, and as antiinflammatory agent From the ethanolic extract of the roots of C sinica, five isoflavonoid compounds were isolated and identified as flemichapparin B, formononetin, pseudobaptigenin, 5 hydroxy 7,4 ′ dimethoxy isoflavone and 5 hydroxy 7 methoxy 3 ′,4 ′ methylenedioxy isoflavone
基金This project is supported by the National Natural Science Foundation of China （No. 39270800）；the Major Subject of the Science and Technology Commission of Shanghai Municipality （No. 06DZ19709）.
文摘A new tetrastilbene named carasinol D （1） was isolated from the roots of Caragana sinica. Its structure was elucidated by spectroscopic analysis. Compound 1 was obtained from carasinol B （2） by an acid-catalyzed conversion.
基金Major State Basic Research Projects of China(973-2010CB732204)Specific Programs in Graduate Science and Technology Innovation of Beijing Forestry University(BLYJ201110)
文摘In order to efficiently explore and use woody biomass,six lignin fractions were isolated from dewaxed Caragana sinica via successive extraction with organic solvents and alkaline solutions.The lignin structures were characterized by Fourier transform infrared spectroscopy(FT-IR) and 1D and 2D Nuclear Magnetic Resonance(NMR).FT-IR spectra revealed that the "core" of the lignin structure did not significantly change during the treatment under the conditions given.The results of 1H and 13C NMR demonstrated that the lignin fraction L2,isolated with 70% ethanol containing 1% NaOH,was mainly composed of β-O-4 ether bonds together with G and S units and trace p-hydroxyphenyl unit.Based on the 2D HSQC NMR spectrum,the ethanol organosolv lignin fraction L1,extracted with 70% ethanol,presents a predominance of β-O-4′ aryl ether linkages(61% of total side chains),and a low abundance of condensed carbon-carbon linked structures(such as ββ′,β-1′,and β-5′) and a lower S/G ratio.Furthermore,a small percentage(ca.9%) of the linkage side chain was found to be acylated at the γ-carbon.
文摘目的探讨金雀根和黄芪配伍对糖尿病肾病(DKD)大鼠的影响。方法将SD大鼠随机分为正常组、模型组、恩格列净组、金雀根组、黄芪组、配伍组,每组10只。采用单侧切除肾脏联合腹腔注射链脲佐菌素(STZ)复制DKD大鼠模型,造模成功后灌胃相应剂量药物8周。第0、4、8周检测24 h尿微量白蛋白(24 h U-mALB);ELISA法检测Scr、BUN、CysC、MDA水平及SOD活性;荧光探针法检测肾组织ROS表达;HE、PAS、Masson、PASM-Masson染色观察肾组织病理结构改变;免疫组织化学法检测肾组织NOX4、Drp1、MFN2、P62表达;Western blot法检测肾组织PINK1、MFN2、Parkin、LC3-Ⅱ/Ⅰ、P62、p-Drp1蛋白表达。结果与模型组比较,各给药组大鼠24 h U-mALB、BUN、Scr、CysC水平均降低(P<0.01),肾组织病理性结构损伤改善;血清和组织MDA水平降低(P<0.01),SOD活性升高(P<0.01);肾组织PINK1、MFN2、Parkin、LC3-Ⅱ/Ⅰ蛋白表达升高(P<0.05,P<0.01),p-Drp1、P62蛋白表达降低(P<0.01),其中配伍组作用优于单用药组(P<0.05,P<0.01)。结论金雀根和黄芪配伍可能是通过调控PINK1/MFN2/Parkin通路改善线粒体动力学,激活线粒体自噬,抑制氧化应激,减轻肾脏病理损伤,改善DKD大鼠肾功能。
