This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using convention...This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using conventional methods,and then were isolated. The further tests and analysis of the isolated strain were developed,including the regression experiment to P. clarkia,the morphology,physiological and biochemical characteristics,sequence analysis of their 16 S rRNA and gyr B genes,and the susceptibility test to antibiotics. Large colonies with similar morphology and color were obtained. Strain X120523 was identified as Citrobacter freundii,proved to have strong pathogenicity,and was susceptible to quinolones and aminoglycosides.展开更多
Medicinal plants have been presented as a valuable source of preservation of human health. In special, Stryphnodendron barbatiman has been employed due to its antimicrobial activity. This plant is rich in tannins and ...Medicinal plants have been presented as a valuable source of preservation of human health. In special, Stryphnodendron barbatiman has been employed due to its antimicrobial activity. This plant is rich in tannins and has been used in popular medicine for the treatment of gastrointestinal disorder, treatment of lesions, and also as anti-inflammatory microbicide. Citrobacter freundii is a member of the family Enterobacteriaceae and is one of the major causes of opportunistic infections. This microorganism is a bacterium (bacillus) aerobic gram-negative with a length in the range of 1 to 5 mm. C. freundii is commonly found in water, soil, food and occasionally in the gastrointestinal tract of animals and humans. In this paper, we have demonstrated the antibacterial activity of S. barbatiman by observing cellular death by using inhibition halo approach. Atomic force microscopy and FT-IR spectroscopy results suggested that interaction between the main active components of S. barbatiman with cellular wall of C. freundii gives rise to cellular wall damage, and then leads this microorganism to death.展开更多
基金Supported by the Science and Technology Innovative Research Team of Anhui Academy of Agricultural Sciences(14C0504)the Youth Innovation Foundation of President of Anhui Academy of Agricultural Sciences(14B0529)Anhui Aquaculture Industry Technology System for Shrimp and Crab
文摘This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using conventional methods,and then were isolated. The further tests and analysis of the isolated strain were developed,including the regression experiment to P. clarkia,the morphology,physiological and biochemical characteristics,sequence analysis of their 16 S rRNA and gyr B genes,and the susceptibility test to antibiotics. Large colonies with similar morphology and color were obtained. Strain X120523 was identified as Citrobacter freundii,proved to have strong pathogenicity,and was susceptible to quinolones and aminoglycosides.
基金This work was supported by CNPq and Capes (Brazil).
文摘Medicinal plants have been presented as a valuable source of preservation of human health. In special, Stryphnodendron barbatiman has been employed due to its antimicrobial activity. This plant is rich in tannins and has been used in popular medicine for the treatment of gastrointestinal disorder, treatment of lesions, and also as anti-inflammatory microbicide. Citrobacter freundii is a member of the family Enterobacteriaceae and is one of the major causes of opportunistic infections. This microorganism is a bacterium (bacillus) aerobic gram-negative with a length in the range of 1 to 5 mm. C. freundii is commonly found in water, soil, food and occasionally in the gastrointestinal tract of animals and humans. In this paper, we have demonstrated the antibacterial activity of S. barbatiman by observing cellular death by using inhibition halo approach. Atomic force microscopy and FT-IR spectroscopy results suggested that interaction between the main active components of S. barbatiman with cellular wall of C. freundii gives rise to cellular wall damage, and then leads this microorganism to death.
