N, O-carboxymethyl chitosan （ CMCTS ）, a kind of biodegradable organic substance, was added to calcium phosphate bone cement （CPC） to prodnce a composite more similar in composition to human bone. The compressive ...N, O-carboxymethyl chitosan （ CMCTS ）, a kind of biodegradable organic substance, was added to calcium phosphate bone cement （CPC） to prodnce a composite more similar in composition to human bone. The compressive strength of the new material was inereased by 10 times compared with conventional CPC.展开更多
INTENTION:The effect of different reaction conditions such as temperature,pH value,time,the concentration of Na+ ions and Pb2+ ions on the complexation capacity of carboxymethyl chitosan（CMCS） to Ca2+ions,were inves...INTENTION:The effect of different reaction conditions such as temperature,pH value,time,the concentration of Na+ ions and Pb2+ ions on the complexation capacity of carboxymethyl chitosan（CMCS） to Ca2+ions,were investigated.METHOD:In different reaction conditions,carboxymethyl chitosan reacted with saturated solution of calcirm chorde,produced carboxymethyl chitoxan calcium（CCC）.The Ca2+ions concentration of CCC was determined by atomic absorption spectrophotometer.RESULT:After 15 minutes,the complexation capacity of Ca2+ions to CMCS was to most in the room temperature at pH6.0.CONCLUSION:The complexation of Ca2+ions to CMCS has been influenced by the complexation capacity of CMCS to other cations in the reactions system.展开更多
The significant role of the polyelectrolytic nature of non-collagenous proteins (NCPs) in regulating the in vivo mineralization of collagen provides important insights for scientists searching for analogues of NCPs to...The significant role of the polyelectrolytic nature of non-collagenous proteins (NCPs) in regulating the in vivo mineralization of collagen provides important insights for scientists searching for analogues of NCPs to achieve in vitro collagen mineralization. Polyampholyte carboxymethyl chitosan (CMC) has both carboxyl and amino groups, which allows it to act as a cationic or anionic polyelectrolyte below or above its isoelectric point (IP), respectively. In this study, CMC was employed as the analogue of NCPs to stabilize amorphous calcium phosphate (ACP) under acidic conditions (pH < 3.5) via the formation of CMC/ACP nanocomplexes. In the presence of both ACP nanoparticles and acid collagen molecules, ACP nanoparticles could be integrated into collagen fibrils during the process of collagen self-assembly and achieve intrafibrillar mineralization of collagen in vitro (i.e., synchronous self-assembly/mineralization (SSM) of collagen). This mode of mineralization is different from established mechanisms in which mineralization follows the self-assembly (MFS) of collagen. Thus, SSM provides a new strategy for developing materials from mineralized collagen scaffolds.展开更多
文摘N, O-carboxymethyl chitosan （ CMCTS ）, a kind of biodegradable organic substance, was added to calcium phosphate bone cement （CPC） to prodnce a composite more similar in composition to human bone. The compressive strength of the new material was inereased by 10 times compared with conventional CPC.
文摘INTENTION:The effect of different reaction conditions such as temperature,pH value,time,the concentration of Na+ ions and Pb2+ ions on the complexation capacity of carboxymethyl chitosan（CMCS） to Ca2+ions,were investigated.METHOD:In different reaction conditions,carboxymethyl chitosan reacted with saturated solution of calcirm chorde,produced carboxymethyl chitoxan calcium（CCC）.The Ca2+ions concentration of CCC was determined by atomic absorption spectrophotometer.RESULT:After 15 minutes,the complexation capacity of Ca2+ions to CMCS was to most in the room temperature at pH6.0.CONCLUSION:The complexation of Ca2+ions to CMCS has been influenced by the complexation capacity of CMCS to other cations in the reactions system.
基金financial support of the project from the National Natural Science Foundation of China (Nos. 31870947 and 81571016)
文摘The significant role of the polyelectrolytic nature of non-collagenous proteins (NCPs) in regulating the in vivo mineralization of collagen provides important insights for scientists searching for analogues of NCPs to achieve in vitro collagen mineralization. Polyampholyte carboxymethyl chitosan (CMC) has both carboxyl and amino groups, which allows it to act as a cationic or anionic polyelectrolyte below or above its isoelectric point (IP), respectively. In this study, CMC was employed as the analogue of NCPs to stabilize amorphous calcium phosphate (ACP) under acidic conditions (pH < 3.5) via the formation of CMC/ACP nanocomplexes. In the presence of both ACP nanoparticles and acid collagen molecules, ACP nanoparticles could be integrated into collagen fibrils during the process of collagen self-assembly and achieve intrafibrillar mineralization of collagen in vitro (i.e., synchronous self-assembly/mineralization (SSM) of collagen). This mode of mineralization is different from established mechanisms in which mineralization follows the self-assembly (MFS) of collagen. Thus, SSM provides a new strategy for developing materials from mineralized collagen scaffolds.
