In order to regenerate myocardium and provide appropriate mechanical support after a heart attack,jersey,tuck and rib stitch structures were knitted from polylactic acid(PLA)yarns to fabricate a cardiac patch,which mi...In order to regenerate myocardium and provide appropriate mechanical support after a heart attack,jersey,tuck and rib stitch structures were knitted from polylactic acid(PLA)yarns to fabricate a cardiac patch,which mimicked the mechanical properties of myocardium in both directions.Cardiosphere-derived cells(CDCs) were seeded on these PLA patch fabrics,and using scanning electron microscopy(SEM) characterization and an MTT assay the cells proliferated and attached successfully to the PLA fabrics.Based on the results,the rib stitch structure is the most promising candidate for fabricating cardiac patches due to its high elasticity and its ability to promote cell proliferation.展开更多
The infarcted heart undergoes irreversible pathological remodeling after reperfusion involving left ventricle dilation and excessive inflammatory reactions in the infarcted heart,frequently leading to fatal functional...The infarcted heart undergoes irreversible pathological remodeling after reperfusion involving left ventricle dilation and excessive inflammatory reactions in the infarcted heart,frequently leading to fatal functional damage.Extensive attempts have been made to attenuate pathological remodeling in infarcted hearts using cardiac patches and anti-inflammatory drug delivery.In this study,we developed a paintable and adhesive hydrogel patch using dextran-aldehyde(dex-ald)and gelatin,incorporating the anti-inflammatory protein,ANGPTL4,into the hydrogel for sustained release directly to the infarcted heart to alleviate inflammation.We optimized the material composition,including polymer concentration and molecular weight,to achieve a paintable,adhesive hydrogel using 10%gelatin and 5%dex-ald,which displayed in-situ gel formation within 135 s,cardiac tissue-like modulus(40.5 kPa),suitable tissue adhesiveness(4.3 kPa),and excellent mechanical stability.ANGPTL4 was continuously released from the gelatin/dex-ald hydrogel without substantial burst release.The gelatin/dex-ald hydrogel could be conveniently painted onto the beating heart and degraded in vivo.Moreover,in vivo studies using animal models of acute myocardial infarction revealed that our hydrogel cardiac patch containing ANGPTL4 significantly improved heart tissue repair,evaluated by echocardiography and histological evaluation.The heart tissues treated with ANGPTL4-loaded hydrogel patches exhibited increased vascularization,reduced inflammatory macrophages,and structural maturation of cardiac cells.Our novel hydrogel system,which allows for facile paintability,appropriate tissue adhesiveness,and sustained release of anti-inflammatory drugs,will serve as an effective platform for the repair of various tissues,including heart,muscle,and cartilage.展开更多
Myocardial infarction(MI)is one of the common cardiovascular diseases that occurs with a blockage in one or more of the coronary arteries to lead to the damage of the myocardium,resulting in a lifethreatening conditio...Myocardial infarction(MI)is one of the common cardiovascular diseases that occurs with a blockage in one or more of the coronary arteries to lead to the damage of the myocardium,resulting in a lifethreatening condition.To repair the damaged myocardium in MI,researchers are looking forwards to new ways to postpone the progression of myocardial injury.Cardiac patches,the scaffolds layered on the heart surface,can provide mechanical support for the infarction site and improve cardiac function by delivering various bioactive factors or cells,showing considerable curative effect in the treatment of MI.Biomaterials with certain biocompatibility and mechanical properties have received widespread attention for the application in cardiac patches.In this review,we focus on the recent progress on these biomaterialsbased cardiac patches,which could be categorized into two types according to the sources of materials including(ⅰ)natural materials and(ⅱ)synthetic materials.The major advantages and current challenges of each type are discussed and a brief perspective on the future research directions is presented.展开更多
Cell implantation offers an appealing avenue for heart repair after myocardial infarction(MI).Nevertheless,the implanted cells are subjected to the aberrant myocardial niche,which inhibits cell survival and maturation...Cell implantation offers an appealing avenue for heart repair after myocardial infarction(MI).Nevertheless,the implanted cells are subjected to the aberrant myocardial niche,which inhibits cell survival and maturation,posing significant challenges to the ultimate therapeutic outcome.The functional cardiac patches(CPs)have been proved to construct an elastic conductive,antioxidative,and angiogenic microenvironment for rectifying the aberrant microenvironment of the infarcted myocardium.More importantly,inducing implanted cardiomyocytes(CMs)adapted to the anisotropic arrangement of myocardial tissue by bioengineered structural cues within CPs are more conducive to MI repair.Herein,a functional Cig/(TA-Cu)CP served as biomimetic cardiac niche was fabricated based on structural anisotropic cigarette filter by modifying with tannic acid(TA)-chelated Cu2+(TA-Cu complex)via a green method.This CP possessed microstructural anisotropy,electrical conductivity and mechanical properties similar to natural myocardium,which could promote elongation,orientation,maturation,and functionalization of CMs.Besides,the Cig/(TA-Cu)CP could efficiently scavenge reactive oxygen species,reduce CM apoptosis,ultimately facilitating myocardial electrical integration,promoting vascular regeneration and improving cardiac function.Together,our study introduces a functional CP that integrates multimodal cues to create a biomimetic cardiac niche and provides an effective strategy for cardiac repair.展开更多
Cardiovascular diseases cause huge socio-economic burden worldwide.Although a mammalian myocardium has its own limited healing capability,scaffold materials capable of releasing stem cell recruiting/engrafting factors...Cardiovascular diseases cause huge socio-economic burden worldwide.Although a mammalian myocardium has its own limited healing capability,scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium.The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P(SP)and insulin-like growth factor-1C(IGF-1C)peptide.Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning,which exhibited nanofibrous morphology.SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro.The developed patches were transplanted in an infarcted myocardium for up to 14 days.Mice underwent left anterior descending artery ligation and received one of the following treatments:(i)sham,(ii)saline,(iii)patch-only,(iv)IGF-1C patch,(v)SP patch and(vi)IGF-1C/SP patch.SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline,patch-only and individual peptide containing cardiac patches.SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups.IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups.Moreover,IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups.These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair.展开更多
基金the College of Textiles,North Carolina State University,Raleigh,USA“111 Project” Biomedical Textile Materials Science and Technology,China(No.B07024)
文摘In order to regenerate myocardium and provide appropriate mechanical support after a heart attack,jersey,tuck and rib stitch structures were knitted from polylactic acid(PLA)yarns to fabricate a cardiac patch,which mimicked the mechanical properties of myocardium in both directions.Cardiosphere-derived cells(CDCs) were seeded on these PLA patch fabrics,and using scanning electron microscopy(SEM) characterization and an MTT assay the cells proliferated and attached successfully to the PLA fabrics.Based on the results,the rib stitch structure is the most promising candidate for fabricating cardiac patches due to its high elasticity and its ability to promote cell proliferation.
基金supported by a grant from the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT,and Future Planning(NRF-2021M3H4A1A04092882 and NRF-2021R1A4A3025206).
文摘The infarcted heart undergoes irreversible pathological remodeling after reperfusion involving left ventricle dilation and excessive inflammatory reactions in the infarcted heart,frequently leading to fatal functional damage.Extensive attempts have been made to attenuate pathological remodeling in infarcted hearts using cardiac patches and anti-inflammatory drug delivery.In this study,we developed a paintable and adhesive hydrogel patch using dextran-aldehyde(dex-ald)and gelatin,incorporating the anti-inflammatory protein,ANGPTL4,into the hydrogel for sustained release directly to the infarcted heart to alleviate inflammation.We optimized the material composition,including polymer concentration and molecular weight,to achieve a paintable,adhesive hydrogel using 10%gelatin and 5%dex-ald,which displayed in-situ gel formation within 135 s,cardiac tissue-like modulus(40.5 kPa),suitable tissue adhesiveness(4.3 kPa),and excellent mechanical stability.ANGPTL4 was continuously released from the gelatin/dex-ald hydrogel without substantial burst release.The gelatin/dex-ald hydrogel could be conveniently painted onto the beating heart and degraded in vivo.Moreover,in vivo studies using animal models of acute myocardial infarction revealed that our hydrogel cardiac patch containing ANGPTL4 significantly improved heart tissue repair,evaluated by echocardiography and histological evaluation.The heart tissues treated with ANGPTL4-loaded hydrogel patches exhibited increased vascularization,reduced inflammatory macrophages,and structural maturation of cardiac cells.Our novel hydrogel system,which allows for facile paintability,appropriate tissue adhesiveness,and sustained release of anti-inflammatory drugs,will serve as an effective platform for the repair of various tissues,including heart,muscle,and cartilage.
基金supported by the National Natural Science Foundation of China(Nos.91839101,21774086)the Natural Science Foundation of Jiangsu Province(No.BK20180093)+4 种基金the Suzhou Municipal Science and Technology Foundation(No.SYS2018026)the Introduction Project of Clinical Medicine Expert Team for Suzhou(No.SZYJTD201704)the Project of Improvement in Clinical Trial Ability of Cardiovascular Group of the First Affiliated Hospital of Soochow University(No.201900180019)the Application Research on New Platelet Function-detecting Technology in Thrombosis Prevention(No.31010303010982)the Priority Academic Program Development of Jiangsu Higher Education。
文摘Myocardial infarction(MI)is one of the common cardiovascular diseases that occurs with a blockage in one or more of the coronary arteries to lead to the damage of the myocardium,resulting in a lifethreatening condition.To repair the damaged myocardium in MI,researchers are looking forwards to new ways to postpone the progression of myocardial injury.Cardiac patches,the scaffolds layered on the heart surface,can provide mechanical support for the infarction site and improve cardiac function by delivering various bioactive factors or cells,showing considerable curative effect in the treatment of MI.Biomaterials with certain biocompatibility and mechanical properties have received widespread attention for the application in cardiac patches.In this review,we focus on the recent progress on these biomaterialsbased cardiac patches,which could be categorized into two types according to the sources of materials including(ⅰ)natural materials and(ⅱ)synthetic materials.The major advantages and current challenges of each type are discussed and a brief perspective on the future research directions is presented.
基金supported by the National Natural Science Foundation of China(Grant No.32071355,U21A20173,32071363,32201083)the National Key R&D program of China(2023YFC2412803,2022YFC2402801)+3 种基金Science and Technology Planning Project of Guangdong Province(2022A1515011888)the Marine Economy Development Project of Department of Natural Resources of Guangdong Province(No.GDNRC[2022]039)China Postdoctoral Fund Program(No.2021M701599)Shenzhen Fundamental Research Key Project(JCYJ20200109150641992).
文摘Cell implantation offers an appealing avenue for heart repair after myocardial infarction(MI).Nevertheless,the implanted cells are subjected to the aberrant myocardial niche,which inhibits cell survival and maturation,posing significant challenges to the ultimate therapeutic outcome.The functional cardiac patches(CPs)have been proved to construct an elastic conductive,antioxidative,and angiogenic microenvironment for rectifying the aberrant microenvironment of the infarcted myocardium.More importantly,inducing implanted cardiomyocytes(CMs)adapted to the anisotropic arrangement of myocardial tissue by bioengineered structural cues within CPs are more conducive to MI repair.Herein,a functional Cig/(TA-Cu)CP served as biomimetic cardiac niche was fabricated based on structural anisotropic cigarette filter by modifying with tannic acid(TA)-chelated Cu2+(TA-Cu complex)via a green method.This CP possessed microstructural anisotropy,electrical conductivity and mechanical properties similar to natural myocardium,which could promote elongation,orientation,maturation,and functionalization of CMs.Besides,the Cig/(TA-Cu)CP could efficiently scavenge reactive oxygen species,reduce CM apoptosis,ultimately facilitating myocardial electrical integration,promoting vascular regeneration and improving cardiac function.Together,our study introduces a functional CP that integrates multimodal cues to create a biomimetic cardiac niche and provides an effective strategy for cardiac repair.
基金supported by the KIST Institutional Program and by the KUKIST Graduate School of Converging Science and Technology Program.Project supported by the National Science Foundation for Young Scientists of China(Grant No.81701839)The Youth Foundation of Tianjin Medical University(Grant No.2015KYZQ14).
文摘Cardiovascular diseases cause huge socio-economic burden worldwide.Although a mammalian myocardium has its own limited healing capability,scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium.The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P(SP)and insulin-like growth factor-1C(IGF-1C)peptide.Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning,which exhibited nanofibrous morphology.SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro.The developed patches were transplanted in an infarcted myocardium for up to 14 days.Mice underwent left anterior descending artery ligation and received one of the following treatments:(i)sham,(ii)saline,(iii)patch-only,(iv)IGF-1C patch,(v)SP patch and(vi)IGF-1C/SP patch.SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline,patch-only and individual peptide containing cardiac patches.SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups.IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups.Moreover,IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups.These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair.