Flixweed seeds were collected from suspected winter wheat fields and remote hillside in Shaanxi Province, China, their sensitivities to tribenuron-methyl were evaluated in the greenhouse. Results revealed that biotype...Flixweed seeds were collected from suspected winter wheat fields and remote hillside in Shaanxi Province, China, their sensitivities to tribenuron-methyl were evaluated in the greenhouse. Results revealed that biotype S was susceptible to tribenuron, and its GRs0 was 0.23 g a.i. ha^-1, whereas biotypes R1, R2, R3, and R4 were resistant to the tribenuron, and their GR50 were 161.99, 79.70, 439.80, and 312.30, respectively. Biotypes R1 and R2 showed moderate resistance with resistant indices （RI） of 615.23 and 302.7, respectively. Whereas biotypes R3 and R4 showed high resistance to the herbicide with RI values as high as 1 670.34 and 1 186.10, respectively.展开更多
Study on relative sensitivity of maize (Zea mays L.) Nongda108 and Nongda3138 to sulfony-lurea herbicide chlorsulfuron and tribenuron-methyl using maize taproot length by sand bioassy indicated that, Nongda3138 had hi...Study on relative sensitivity of maize (Zea mays L.) Nongda108 and Nongda3138 to sulfony-lurea herbicide chlorsulfuron and tribenuron-methyl using maize taproot length by sand bioassy indicated that, Nongda3138 had higher tolerance to chlorsulfuron and tribenuron-methyl than Nongda108 did. Chlorsulfuron had stronger growth inhibition to maize Nongda108 and Nongda3138 than tribenuron-methyl did. Study on target enzyme of sulfonylurea herbicide acetolactate synthase (ALS) showed that, chlorsulfuron and tribenuron-methyl inhibited ALS in vitro strongly, and non-competitively. In the same concentration of inhibitors, chlorsulfuron had stronger ALS activity inhibition than tribenuron-methyl did. Lower level of chlorsulfuron and tribenuron-methyl has no ALS activity inhibition in vivo, the ALS inhibition only occurred in the condition of high concentration of chlorsulfuron and tribenuron-methyl in vivo.展开更多
Shepherd＇s purse seeds were collected from winter wheat fields and road sides in Hebei Province,China.Their sensitivities to tribenuron-methyl were evaluated in a whole plant dose response assay in the greenhouse.Res...Shepherd＇s purse seeds were collected from winter wheat fields and road sides in Hebei Province,China.Their sensitivities to tribenuron-methyl were evaluated in a whole plant dose response assay in the greenhouse.Results revealed that HB-1,HB-2,HB-3,HB-4,HB-6,HB-7,HB-17,HB-18,HB-19,HB-20,HB-21,and HB-22 populations were susceptible to tribenuron-methyl characterised by very low GR50 values of about 0.10 g a.i.ha-1.On the other hand,populations HB-15 and HB-16 had moderate resistance to tribenuron-methyl with resistance indices （RI） ranging from 10-100 folds,whilst populations HB-5,HB-8,HB-9,HB-10,HB-11,HB-12,HB-13,and HB-14 expressed high level of resistance to tribenuron-methyl with resistance indices over 100.The proportion of moderate resistance level was about 9.09%,and that of high resistance was about 36.36%.展开更多
[Objectives]This study was conducted to provide a basis for the rapid identification of the drug spraying effect in early stage and the molecular mechanism of chemical hybridizing in Brassica napus L.[Methods]Quantita...[Objectives]This study was conducted to provide a basis for the rapid identification of the drug spraying effect in early stage and the molecular mechanism of chemical hybridizing in Brassica napus L.[Methods]Quantitative RT-PCR analysis showed that ALS was constitutively expressed in various tissues of 096030,including flower buds,four floral organs (calyxes,petals,stamens and pistils),roots,stems and leaves.ALS was prominently expressed in leaves and was expressed weakly in the petals and stamens.The male sterility-inducing effects of tribenuron-methyl on such two Brassica napus L.varieties as Ningyou18 and 096030 were investigated.[Results]Plants were twice sprayed with 0.2 μg/ml tribenuron-methyl on leaves.The results showed that 8-10 ml of tribenuron- methyl was applied per plant for the first time at bolting stage with 1-2 mm flower buds on 15-20 cm inflorescence,and the second spray was performed with 8-10 ml of tribenuron-methyl per plant 10 d later.The results showed that the percentage of the full sterile plants reached 100%,which lasted for the whole flowering period,and the relative seed setting rate was only about 4%.Thus,this method could fullfill the requirement of hybrid seed production in field.The in-vivo enzyme activity of acetolactate synthase (ALS) was assayed using 2 mm buds collected 3 d after spray.The results showed that 0.2 μg/ml tribenuron-methyl inhibited ALS activity.The ALS activity of Ningyou 18 (CK) and Ningyou 18 (0.2 μg/ml) was 3.20 and 1.30 μmol/(mg·h),respectively,and the ALS activity of 096030 (CK) and 096030 (0.2 μg/ml) was 3.37 and 1.25 μmol/(mg·h),respectively.The relative enzyme activity of ALS in Ningyou18 and 096030 was 40.63% and 37.23%,respectively,both of which decreased significantly.[Conclusions]These results showed that the change of ALS activity may be used as an index for quickly identifying and predicting the chemical hybridizing effect of tribenuron-methyl.展开更多
基金funded by the National Key Technology R&D Program of China (2006BAD08A09)
文摘Flixweed seeds were collected from suspected winter wheat fields and remote hillside in Shaanxi Province, China, their sensitivities to tribenuron-methyl were evaluated in the greenhouse. Results revealed that biotype S was susceptible to tribenuron, and its GRs0 was 0.23 g a.i. ha^-1, whereas biotypes R1, R2, R3, and R4 were resistant to the tribenuron, and their GR50 were 161.99, 79.70, 439.80, and 312.30, respectively. Biotypes R1 and R2 showed moderate resistance with resistant indices （RI） of 615.23 and 302.7, respectively. Whereas biotypes R3 and R4 showed high resistance to the herbicide with RI values as high as 1 670.34 and 1 186.10, respectively.
文摘Study on relative sensitivity of maize (Zea mays L.) Nongda108 and Nongda3138 to sulfony-lurea herbicide chlorsulfuron and tribenuron-methyl using maize taproot length by sand bioassy indicated that, Nongda3138 had higher tolerance to chlorsulfuron and tribenuron-methyl than Nongda108 did. Chlorsulfuron had stronger growth inhibition to maize Nongda108 and Nongda3138 than tribenuron-methyl did. Study on target enzyme of sulfonylurea herbicide acetolactate synthase (ALS) showed that, chlorsulfuron and tribenuron-methyl inhibited ALS in vitro strongly, and non-competitively. In the same concentration of inhibitors, chlorsulfuron had stronger ALS activity inhibition than tribenuron-methyl did. Lower level of chlorsulfuron and tribenuron-methyl has no ALS activity inhibition in vivo, the ALS inhibition only occurred in the condition of high concentration of chlorsulfuron and tribenuron-methyl in vivo.
基金supported by the State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection (IPP),Chinese Academy of Agricultural Sciences,China (SKL2010OP15)the National Natural Science Foundation of China (31000856)
文摘Shepherd＇s purse seeds were collected from winter wheat fields and road sides in Hebei Province,China.Their sensitivities to tribenuron-methyl were evaluated in a whole plant dose response assay in the greenhouse.Results revealed that HB-1,HB-2,HB-3,HB-4,HB-6,HB-7,HB-17,HB-18,HB-19,HB-20,HB-21,and HB-22 populations were susceptible to tribenuron-methyl characterised by very low GR50 values of about 0.10 g a.i.ha-1.On the other hand,populations HB-15 and HB-16 had moderate resistance to tribenuron-methyl with resistance indices （RI） ranging from 10-100 folds,whilst populations HB-5,HB-8,HB-9,HB-10,HB-11,HB-12,HB-13,and HB-14 expressed high level of resistance to tribenuron-methyl with resistance indices over 100.The proportion of moderate resistance level was about 9.09%,and that of high resistance was about 36.36%.
基金Supported by National Natural Science Foundation of China(31571710)Earmarked Fund for China Agriculture Research System(CARS-12)
文摘[Objectives]This study was conducted to provide a basis for the rapid identification of the drug spraying effect in early stage and the molecular mechanism of chemical hybridizing in Brassica napus L.[Methods]Quantitative RT-PCR analysis showed that ALS was constitutively expressed in various tissues of 096030,including flower buds,four floral organs (calyxes,petals,stamens and pistils),roots,stems and leaves.ALS was prominently expressed in leaves and was expressed weakly in the petals and stamens.The male sterility-inducing effects of tribenuron-methyl on such two Brassica napus L.varieties as Ningyou18 and 096030 were investigated.[Results]Plants were twice sprayed with 0.2 μg/ml tribenuron-methyl on leaves.The results showed that 8-10 ml of tribenuron- methyl was applied per plant for the first time at bolting stage with 1-2 mm flower buds on 15-20 cm inflorescence,and the second spray was performed with 8-10 ml of tribenuron-methyl per plant 10 d later.The results showed that the percentage of the full sterile plants reached 100%,which lasted for the whole flowering period,and the relative seed setting rate was only about 4%.Thus,this method could fullfill the requirement of hybrid seed production in field.The in-vivo enzyme activity of acetolactate synthase (ALS) was assayed using 2 mm buds collected 3 d after spray.The results showed that 0.2 μg/ml tribenuron-methyl inhibited ALS activity.The ALS activity of Ningyou 18 (CK) and Ningyou 18 (0.2 μg/ml) was 3.20 and 1.30 μmol/(mg·h),respectively,and the ALS activity of 096030 (CK) and 096030 (0.2 μg/ml) was 3.37 and 1.25 μmol/(mg·h),respectively.The relative enzyme activity of ALS in Ningyou18 and 096030 was 40.63% and 37.23%,respectively,both of which decreased significantly.[Conclusions]These results showed that the change of ALS activity may be used as an index for quickly identifying and predicting the chemical hybridizing effect of tribenuron-methyl.
