Objective: To observe tissue distribution and cell localization of TNF α mRNA and its protein and study their role in the pathogenesis of liver injury in burn rats. Methods: An animal model of rats subjected to 2...Objective: To observe tissue distribution and cell localization of TNF α mRNA and its protein and study their role in the pathogenesis of liver injury in burn rats. Methods: An animal model of rats subjected to 20% TBSA Ⅲ degree burns combined with intraperitoneal injection of lipopolysaccharide (LPS) was used for this experiment. The changes of hepatic morphology and functions and serum TNF α content and expression and localization of liver TNF α and TNF α mRNA were determined with light microscope (LM) and electron microscope (EM), quantitative analysis, immunohistochemistry (IHC) and in situ hybridization (ISH). Results: It showed that there were sinusoid reaction, KCs activation and degeneration, necrosis of HCs, and platelets aggregation, fibrins deposition and PMNs attachment in sinusoid. The activity of ALT was obviously elevated and ALB content was slightly decreased. The serum content of TNF α showed peak at 3 hours. TNF α was mainly localized in sinusoid endothelial cells (SECs) and Kupffer cells (KCs), and TNF α mRNA was mainly distributed in KCs, polymorphonuclears neutrophils (PMNs) and macrophages (MPs). Conclusions: It suggests that TNF α mRNA and its protein expression and localization are coincident with the pathological changes of liver injury. TNF α is one of the key cytokines in the pathogenesis of liver injury in burn rats with endotoxemia.展开更多
文摘Objective: To observe tissue distribution and cell localization of TNF α mRNA and its protein and study their role in the pathogenesis of liver injury in burn rats. Methods: An animal model of rats subjected to 20% TBSA Ⅲ degree burns combined with intraperitoneal injection of lipopolysaccharide (LPS) was used for this experiment. The changes of hepatic morphology and functions and serum TNF α content and expression and localization of liver TNF α and TNF α mRNA were determined with light microscope (LM) and electron microscope (EM), quantitative analysis, immunohistochemistry (IHC) and in situ hybridization (ISH). Results: It showed that there were sinusoid reaction, KCs activation and degeneration, necrosis of HCs, and platelets aggregation, fibrins deposition and PMNs attachment in sinusoid. The activity of ALT was obviously elevated and ALB content was slightly decreased. The serum content of TNF α showed peak at 3 hours. TNF α was mainly localized in sinusoid endothelial cells (SECs) and Kupffer cells (KCs), and TNF α mRNA was mainly distributed in KCs, polymorphonuclears neutrophils (PMNs) and macrophages (MPs). Conclusions: It suggests that TNF α mRNA and its protein expression and localization are coincident with the pathological changes of liver injury. TNF α is one of the key cytokines in the pathogenesis of liver injury in burn rats with endotoxemia.
