The DNA was isolated from banana bunchy top virus （BBTV） in Guangzhou areas and a l.lkb DNA of replicas of BBTV was obtained by PCR using this virus DNA as templates for amplification. This reformed DNA of the BBTV ...The DNA was isolated from banana bunchy top virus （BBTV） in Guangzhou areas and a l.lkb DNA of replicas of BBTV was obtained by PCR using this virus DNA as templates for amplification. This reformed DNA of the BBTV replicas which being homologous to 90% with the Australia＇s encoded the C-Terminal of BBTV replicas. The reformed BBTV replicas were cloned to pBI121 in the position between CaMV 35S promoter and NOS termination sequence, and a plant-expressed carder was established. Four transgenic bananas with the expression of BBTV replicase gene in To generation were detected with PCR and Western blot analysis. The ability of these transgenic bananas against bunchy top virus is being analyzed.展开更多
基金This work was supported by Guangdong Natural Science Foundation in China.
文摘The DNA was isolated from banana bunchy top virus （BBTV） in Guangzhou areas and a l.lkb DNA of replicas of BBTV was obtained by PCR using this virus DNA as templates for amplification. This reformed DNA of the BBTV replicas which being homologous to 90% with the Australia＇s encoded the C-Terminal of BBTV replicas. The reformed BBTV replicas were cloned to pBI121 in the position between CaMV 35S promoter and NOS termination sequence, and a plant-expressed carder was established. Four transgenic bananas with the expression of BBTV replicase gene in To generation were detected with PCR and Western blot analysis. The ability of these transgenic bananas against bunchy top virus is being analyzed.
