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Utilization of Chickpea Split (Cicer arietinum L.) in Preparing Some Gluten-Free Casein-Free Food Products for Autism Children
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作者 Rehab Mohamed Ibrahim 《Food and Nutrition Sciences》 2022年第3期284-315,共32页
This study aimed to prepare and evaluate some gluten-free and casein-free (GFCF) food products for autism children from rice and chickpea split. Like-milk beverages and snacks (bakery) were prepared by replacing rice ... This study aimed to prepare and evaluate some gluten-free and casein-free (GFCF) food products for autism children from rice and chickpea split. Like-milk beverages and snacks (bakery) were prepared by replacing rice with chickpea at a ratio of 25%, 50%, 75%, and 100%, and in a ratio of 25% and 50% with fried snacks. Chemical composition, antioxidant activity, the energy content of ingredients and final products, as well as the viscosity, texture profile analysis, and sensory evaluation of final products, were determined. The results showed that chickpea contains higher values of protein, fat, fiber, and ash compared with rice. Also, the antioxidant activity (total phenolic (TP), DPPH scavenging activity, and FRAP value) of chickpea was higher than rice. The addition of chickpea to rice caused a significant increase in protein (%), fat (%), minerals (Ca, Fe, K, Zn, and Mg) (%), and antioxidant activity of all products, and these values were increased with the increased of chickpea amount added, while the viscosity of rice-chickpea milk samples and the hardness of snacks (fried and bakery) were significantly decreased with the increase of chickpea amount added. According to the recommended daily allowances (RDA), it was found that 100 mL of chickpea milk (100%) could provide autism children with 99.5%, 32%, and 36% of the daily required iron, Ca, and Zn, respectively. Also, the daily intake of 100 g of snacks (sample BS5) could provide autism children with 75%, 7%, 42%, 125%, 1.7%, and 52% of the daily required of protein, fiber, Ca, iron, Mg, and Zn, respectively. On the other hand, 100 g fried snacks (sample FS3) could provide autism children with 59.9%, 42%, and 64% of the daily required protein, calcium, and iron, respectively. The best sensory evaluation scores were obtained with rice milk (100%), bakery snacks sample BS4 (25% rice: 75% chickpea), and fried snacks sample FS2 (75% rice: 25% chickpea). 展开更多
关键词 AUTISM Chickpea Split Rice Gluten-Free Diet casein-free Diet Antioxidants
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不同分子量酪蛋白肽对自由基清除作用研究 被引量:14
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作者 陈东平 牟光庆 《中国酿造》 CAS 北大核心 2010年第4期33-35,共3页
采用超滤法结合凝胶过滤色谱法,分析了不同分子量范围酪蛋白肽对生物体内自由基清除效果的影响。结果表明:酪蛋白肽分子量越小,对生物体内自由基清除率越高,抗氧化效果越好。分子量<1ku时,对羟自由基最高清除率为73.33%,DPPH最高清... 采用超滤法结合凝胶过滤色谱法,分析了不同分子量范围酪蛋白肽对生物体内自由基清除效果的影响。结果表明:酪蛋白肽分子量越小,对生物体内自由基清除率越高,抗氧化效果越好。分子量<1ku时,对羟自由基最高清除率为73.33%,DPPH最高清除率为90.94%。 展开更多
关键词 酪蛋白肽 自由基 分子量
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无皂乳液聚合法制备改性酪素乳液的研究进展 被引量:2
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作者 乔滢寰 马建中 +1 位作者 徐群娜 周建华 《现代化工》 CAS CSCD 北大核心 2013年第2期15-19,共5页
介绍了酪素的结构、性质及改性方法,着重论述了无皂乳液聚合法在酪素改性中的研究进展,并就该方面研究前景提出了展望。
关键词 酪素 无皂乳液聚合法 改性
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分子量对酪蛋白多肽抗氧化活性的影响 被引量:9
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作者 陈敏 敖静 李博 《食品工业科技》 CAS CSCD 北大核心 2012年第9期95-99,共5页
在对酪蛋白酶解产物制备工艺进行优化的基础上,对不同分子量抗氧化肽(>3ku,1~3ku,<1ku)的抗氧化活性进行了评价。首先对酶的种类、酶底物比及水解时间进行了单因素实验,最终确定采用碱性蛋白酶,在pH8.0,55℃,底物浓度5%,酶底物比... 在对酪蛋白酶解产物制备工艺进行优化的基础上,对不同分子量抗氧化肽(>3ku,1~3ku,<1ku)的抗氧化活性进行了评价。首先对酶的种类、酶底物比及水解时间进行了单因素实验,最终确定采用碱性蛋白酶,在pH8.0,55℃,底物浓度5%,酶底物比0.192AU/g的条件下,酶解4h所得水解物抗氧化活性最高。经过超滤和凝胶过滤层析分离获得不同分子量的抗氧化肽,并采用2,2′-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)二铵盐(ABTS+.)、羟自由基和超氧自由基清除活性评价其抗氧化性。结果表明,ABTS+.清除活性与分子量呈负相关(r=-0.898,p<0.01),分子量低于1ku组分活性最强(2mg/mL,Trolox当量为2.08±0.05mmol/L);分子量低于3ku的抗氧化肽羟自由基清除活性较高(IC50:1~3ku,4.43±0.03mg/mL;<1ku,4.35±0.06mg/mL);分子量高于3ku组分主要分布在3~5ku,超氧自由基清除活最强(10mg/mL,66.1%±1.0%)。 展开更多
关键词 酪蛋白 抗氧化肽 分子量 自由基
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无皂法制备有机硅/丙烯酸酯共改性酪素皮革涂饰剂 被引量:7
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作者 乔滢寰 马建中 +1 位作者 徐群娜 周建华 《中国皮革》 CAS 北大核心 2013年第3期30-34,共5页
以酪素、甲基丙烯酸甲酯、丙烯酸丁酯、γ-甲基丙烯酰氧基丙基三甲氧基硅烷(KH-570)为主要原料,采用无皂法制备了有机硅/丙烯酸酯共改性酪素皮革涂饰剂。考察了KH-570的加入方式和用量对乳液稳定性(离心稳定性、耐化学稳定性及水稀释稳... 以酪素、甲基丙烯酸甲酯、丙烯酸丁酯、γ-甲基丙烯酰氧基丙基三甲氧基硅烷(KH-570)为主要原料,采用无皂法制备了有机硅/丙烯酸酯共改性酪素皮革涂饰剂。考察了KH-570的加入方式和用量对乳液稳定性(离心稳定性、耐化学稳定性及水稀释稳定性)及其成膜性能(抗张强度、断裂伸长率、吸水率)的影响,并采用红外(FT-IR)、动态激光光散射(DLS)和透射电镜(TEM)对乳液进行表征。结果表明:采用半连续种子乳液聚合法,KH-570用量为所加总固物质量的6%时,乳液及胶膜的综合性能相对最好。FT-IR结果显示,KH-570成功接枝到酪素分子上;DLS和TEM结果表明,乳胶粒有明显的核壳结构,粒径为纳米级,约为63.47nm左右,且大小分布均一。 展开更多
关键词 酪素 有机硅 无皂乳液 皮革涂饰剂
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阳离子酪素乳液的合成及其性能研究 被引量:1
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作者 张元霞 马建中 徐群娜 《陕西科技大学学报》 CAS 2017年第3期42-46,52,共6页
以天然产物酪素(CA)为基材,通过自由基聚合在酪素体系中引入阳离子单体一甲基丙烯酸二甲氨基乙酯(DMAEMA),获得了结构稳定、性能优异的阳离子酪素乳液.采用红外光谱(FT-IR)和动态光散射粒度仪(DLS)和最低成膜温度测试仪等对乳液的结构... 以天然产物酪素(CA)为基材,通过自由基聚合在酪素体系中引入阳离子单体一甲基丙烯酸二甲氨基乙酯(DMAEMA),获得了结构稳定、性能优异的阳离子酪素乳液.采用红外光谱(FT-IR)和动态光散射粒度仪(DLS)和最低成膜温度测试仪等对乳液的结构和性能进行了表征,通过菌落定量计数法对阳离子酪素的抗菌性进行了考察,并对其涂饰革样的综合性能进行考察.结果显示:阳离子单体已成功接枝到酪素的链段上;当酪素与阳离子单体质量比为1∶1.5时,可获得电位为正,平均粒径为214.2nm的乳液;阳离子酪素乳液最低成膜温度为23.8℃;采用阳离子酪素涂饰革样后,皮革的耐干湿擦性和柔软度得到了提高;并且与纯酪素相比,阳离子酪素对金黄色葡萄球菌具有良好的抗菌效果,其抑菌率约为80%. 展开更多
关键词 酪素 阳离子单体 自由基聚合 抗菌
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罗曼公母鸡内源氨基酸排泄量的比较研究 被引量:1
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作者 宋春玲 马秋刚 +1 位作者 易中华 计成 《中国畜牧杂志》 CAS 北大核心 2007年第19期26-28,共3页
试验选取28周龄健康的罗曼公母鸡各16只,分别分为2个处理,每处理8个重复,每个重复1只鸡,分别饲喂2种试验日粮,即无氮日粮(NFD)和NFD+5%酪蛋白,比较分析2种方法测得的公母鸡内源氨基酸排泄量(EAAL)的差异。结果表明:采用NFD法和NFD+5%酪... 试验选取28周龄健康的罗曼公母鸡各16只,分别分为2个处理,每处理8个重复,每个重复1只鸡,分别饲喂2种试验日粮,即无氮日粮(NFD)和NFD+5%酪蛋白,比较分析2种方法测得的公母鸡内源氨基酸排泄量(EAAL)的差异。结果表明:采用NFD法和NFD+5%酪蛋白所得的公母鸡内源氨基酸和总蛋白质的排泄量有所不同。酪蛋白组能够增加内源氮和内源氨基酸的排泄量,NFD+5%酪蛋白法所测得的内源氮和内源氨基酸排泄量更接近试鸡的真实情况。2种测定方法公鸡内源氮和内源氨基酸的排泄量均高于母鸡的结果。 展开更多
关键词 无氮日粮 酪蛋白 内源氨基酸排泄量 母鸡
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酪蛋白抗氧化肽制备工艺及酶解产物特性研究 被引量:9
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作者 高婷 沈浥 卢蓉蓉 《食品工业科技》 CAS CSCD 北大核心 2010年第6期192-195,198,共5页
采用不同蛋白酶酶解酪蛋白,确定AS1,398中性蛋白酶是酶解酪蛋白制备抗氧化肽的优良酶源。通过单因素和响应面回归分析,得到AS1,398中性蛋白酶酶解酪蛋白的优化工艺条件为:底物浓度30mg/mL,水解时间90min,pH7.2,酶底比为2%(w/w),温度为5... 采用不同蛋白酶酶解酪蛋白,确定AS1,398中性蛋白酶是酶解酪蛋白制备抗氧化肽的优良酶源。通过单因素和响应面回归分析,得到AS1,398中性蛋白酶酶解酪蛋白的优化工艺条件为:底物浓度30mg/mL,水解时间90min,pH7.2,酶底比为2%(w/w),温度为50℃。优化酶解条件下,10mg/mL酶解产物的DPPH自由基清除率为80.43%,IC_(50)为3.96mg/mL,显现出良好的抗氧化性能。高效液相排阻色谱(HPSEC)分析表明,优化条件下产物主要是3500Da以下的一些短肽。 展开更多
关键词 酪蛋白 抗氧化肽 酶解产物 DPPH自由基 响应面优化法
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不同蛋白源供应形式对蛋鸡小肠肽载体PepT1表达的影响 被引量:1
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作者 梁陈冲 陈宝江 李绍华 《中国家禽》 北大核心 2011年第22期11-14,共4页
为研究不同蛋白源形式对蛋鸡肠道肽载体PepT1表达的影响,选取遗传背景相同的成年蛋鸡30只,随机分为3组,每组10只,分别应用游离氨基酸、小肽、酪蛋白为氮源,根据总能和总氮相同的原则,配制不同蛋白形态的纯合日粮,饲喂2周,应用RT-PCR技... 为研究不同蛋白源形式对蛋鸡肠道肽载体PepT1表达的影响,选取遗传背景相同的成年蛋鸡30只,随机分为3组,每组10只,分别应用游离氨基酸、小肽、酪蛋白为氮源,根据总能和总氮相同的原则,配制不同蛋白形态的纯合日粮,饲喂2周,应用RT-PCR技术检测小肠内肽转运载体表达量变化。结果表明:以小肽为氮源的试验组蛋鸡小肠不同肠段转运载体表达量较游离氨基酸和酪蛋白组明显提高(P<0.05),而游离氨基酸组和酪蛋白组间差异不显著(P>0.05)。 展开更多
关键词 游离氨基酸 酪蛋白 小肽 肽转运载体
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Role of gastrointestinal health in managing children with autism spectrum disorder 被引量:2
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作者 Mohammed Al-Beltagi Nermin Kamal Saeed +2 位作者 Adel Salah Bediwy Reem Elbeltagi Rawan Alhawamdeh 《World Journal of Clinical Pediatrics》 2023年第4期171-196,共26页
Children with autism spectrum disorders(ASD)or autism are more prone to gastrointestinal(GI)disorders than the general population.These disorders can significantly affect their health,learning,and development due to v... Children with autism spectrum disorders(ASD)or autism are more prone to gastrointestinal(GI)disorders than the general population.These disorders can significantly affect their health,learning,and development due to various factors such as genetics,environment,and behavior.The causes of GI disorders in children with ASD can include gut dysbiosis,immune dysfunction,food sensitivities,digestive enzyme deficiencies,and sensory processing differences.Many studies suggest that numerous children with ASD experience GI problems,and effective management is crucial.Diagnosing autism is typically done through genetic,neurological,functional,and behavioral assessments and observations,while GI tests are not consistently reliable.Some GI tests may increase the risk of developing ASD or exacerbating symptoms.Addressing GI issues in individuals with ASD can improve their overall well-being,leading to better behavior,cognitive function,and educational abilities.Proper management can improve digestion,nutrient absorption,and appetite by relieving physical discomfort and pain.Alleviating GI symptoms can improve sleep patterns,increase energy levels,and contribute to a general sense of well-being,ultimately leading to a better quality of life for the individual and improved family dynamics.The primary goal of GI interventions is to improve nutritional status,reduce symptom severity,promote a balanced mood,and increase patient independence. 展开更多
关键词 Gastrointestinal disorders Autism spectrum disorders Children Gut microbiota Ketogenic diet Gluten-free casein-free diet Dietary management
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不同非含氟护牙剂对牙釉质酸蚀后再矿化效果影响的体内试验 被引量:7
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作者 伍廷芸 王德堂 +4 位作者 朱友家 阮琼 伍爱民 何尚群 曾小芳 《中国组织工程研究》 CAS 北大核心 2019年第18期2842-2846,共5页
背景:低龄儿童吞咽反射发育尚未健全,使用含氟牙膏存在氟中毒危险,而无氟护齿剂因不含氟元素而具备非常优良的安全性能。目的:比较奥乐V护牙剂、GC护牙素和无氟牙膏在家庭自我口腔保健中对于脱矿牙釉质的再矿化效果。方法:纳入荆门市康... 背景:低龄儿童吞咽反射发育尚未健全,使用含氟牙膏存在氟中毒危险,而无氟护齿剂因不含氟元素而具备非常优良的安全性能。目的:比较奥乐V护牙剂、GC护牙素和无氟牙膏在家庭自我口腔保健中对于脱矿牙釉质的再矿化效果。方法:纳入荆门市康复医院口腔科因正畸需要拟拔除健康第一前磨牙的患者15例,共60颗牙,年龄12-18岁。将每例患者左侧的上下颌第一前磨牙作为对照组,颊面酸蚀后即刻拔除;无氟牙膏组随机选择5例患者,右侧上下颌第一前磨牙颊面酸蚀后,早晚以无氟牙膏刷牙,10 d后拔除;奥乐V护牙剂组随机选择5例患者,右侧上下颌第一前磨牙颊面酸蚀后,早晚以奥乐V护牙剂刷牙,10 d后拔除;GC护牙素组的5例患者,右侧上下颌第一前磨牙颊面酸蚀后,早晚使用含摩擦剂和发泡剂的普通牙膏刷牙后,将GC护牙素均匀涂于酸蚀牙颊面3 min,10 d后拔除。制备离体牙颊面牙釉质标本,进行扫描电镜观察与X射线能谱分析。结果与结论:①扫描电镜显示,对照组牙釉质表面不规则,多孔隙,呈蜂窝状改变,釉柱中心溶解,大部分出现孔隙样凹陷;无氟牙膏组牙釉质表面可见散在的少量矿物质沉积,奥乐V护牙剂组和GC护牙素组牙釉质表面仍有大量蜂窝状空隙,同时有大量矿物质沉积,孔隙变小,矿化物颗粒细小,沉积不均匀,GC护牙素组沉积量较多;②X射线能谱分析显示,对照组与无氟牙膏组钙、磷含量无差异(P> 0.05),奥乐V护牙剂组和GC护牙素组钙、磷含量无差异(P> 0.05),奥乐V护牙剂组、GC护牙素组钙、磷含量高于对照组、无氟牙膏组(P <0.05);③结果表明,奥乐V护牙剂、GC护牙素均可显著促进脱矿牙釉质的再矿化,以GC护牙素的再矿化效果更优。 展开更多
关键词 护牙剂 生物活性玻璃 酪蛋白磷酸肽 无定形磷酸钙 奥乐V护牙剂 GC护牙素 无氟牙膏 再矿化
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Dietary approach and gut microbiota modulation for chronic hepatic encephalopathy in cirrhosis 被引量:10
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作者 Daniela Campion Ilaria Giovo +3 位作者 Paola Ponzo Giorgio M Saracco Federico Balzola Carlo Alessandria 《World Journal of Hepatology》 CAS 2019年第6期489-512,共24页
Hepatic encephalopathy(HE)is a common and serious neuropsychiatric complication of cirrhosis,acute liver failure,and porto-systemic shunting.HE largely contributes to the morbidity of patients with liver disease,sever... Hepatic encephalopathy(HE)is a common and serious neuropsychiatric complication of cirrhosis,acute liver failure,and porto-systemic shunting.HE largely contributes to the morbidity of patients with liver disease,severely affecting the quality of life of both patients and their relatives and being associated with poor prognosis.Its presentation is largely variable,manifesting with a broad spectrum of cognitive abnormalities ranging from subtle cognitive impairment to coma.The pathogenesis of HE is complex and has historically been linked with hyperammonemia.However,in the last years,it has become evident that the interplay of multiple actors,such as intestinal dysbiosis,gut hyperpermeability,and neuroinflammation,is of crucial importance in its genesis.Therefore,HE can be considered a result of a dysregulated gut-liverbrain axis function,where cognitive impairment can be reversed or prevented by the beneficial effects induced by“gut-centric”therapies,such as non-absorbable disaccharides,non-absorbable antibiotics,probiotics,prebiotics,and fecal microbiota transplantation.In this context dietary modifications,by modulating the intestinal milieu,can also provide significant benefit to cirrhotic patients with HE.This review will provide a comprehensive insight into the mechanisms responsible for gut-liver-brain axis dysregulation leading to HE in cirrhosis.Furthermore,it will explore the currently available therapies and the most promising future treatments for the management of patients with HE,with a special focus on the dietary approach. 展开更多
关键词 CIRRHOSIS Hepatic encephalopathy DIET therapy GUT MICROBIOTA Leaky GUT HYPERAMMONEMIA Prebiotics Probiotics Gluten-casein free DIET GUT MICROBIOTA transplantation
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酪蛋白糖巨肽酶解产物抗氧化活性及其对RAW 264.7细胞氧化损伤的保护作用
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作者 唐麒雯 吴泽仪 +3 位作者 余宁翔 叶沁 孟祥河 聂小华 《食品与发酵工业》 CAS CSCD 北大核心 2021年第19期95-100,共6页
采用中性蛋白酶、木瓜蛋白酶和碱性蛋白酶制备酪蛋白糖巨肽(glycomacropeptide,GMP)酶解产物,基于自由基清除能力确定适宜的蛋白酶种类与酶解时间,进而评价其对H_(2)O_(2)诱导RAW_(2)64.7细胞存活率和活性氧(reactive oxygen species,R... 采用中性蛋白酶、木瓜蛋白酶和碱性蛋白酶制备酪蛋白糖巨肽(glycomacropeptide,GMP)酶解产物,基于自由基清除能力确定适宜的蛋白酶种类与酶解时间,进而评价其对H_(2)O_(2)诱导RAW_(2)64.7细胞存活率和活性氧(reactive oxygen species,ROS)生成量的影响。结果表明,不同蛋白酶制备的GMP酶解产物均可显著清除ABTS阳离子自由基、DPPH自由基和·O^(-)_(2);其中中性蛋白酶酶解4 h的酶解产物(neutral protease-glycomacropeptide hydrolysates,N-GMPH)具有较强的自由基清除能力;同时N-GMPH可显著提高H_(2)O_(2)诱导的RAW_(2)64.7细胞存活率并降低ROS生成量。研究显示,N-GMPH具有缓解细胞氧化损伤的作用,该研究可为GMP酶解产物进一步开发功能食品配料提供研究依据。 展开更多
关键词 酪蛋白糖巨肽 酶解产物 自由基清除能力 抗氧化损伤 RAW_(2)64.7细胞
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酶解酪蛋白产生抗氧化多肽的工艺研究 被引量:1
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作者 黄靖怡 柯德森 《广东化工》 CAS 2019年第11期1-3,共3页
为探究水解条件对产物抗氧化活性的影响,采用NBT光还原法,以超氧阴离子自由基清除能力为检测指标,筛选酪蛋白酶解产生抗氧化多肽的最佳蛋白酶,并采用正交试验对酪蛋白水解条件进行优化,最后通过验证试验对优化条件下的酶解产物的超氧阴... 为探究水解条件对产物抗氧化活性的影响,采用NBT光还原法,以超氧阴离子自由基清除能力为检测指标,筛选酪蛋白酶解产生抗氧化多肽的最佳蛋白酶,并采用正交试验对酪蛋白水解条件进行优化,最后通过验证试验对优化条件下的酶解产物的超氧阴离子自由基、DPPH·自由基和羟自由基清除能力进行评价,以获得具有理想综合抗氧化活力的水解条件组合。结果表明:胰蛋白酶酶解酪蛋白所得产物显示出最高超氧阴离子自由基清除能力。在底物浓度10%、55℃、pH=6.5、反应时间4h、酶用量35μL的条件获得了抗氧化活性较高的蛋白水解产物,且该产物也具有较理想的DPPH·自由基和羟自由基清除能力。 展开更多
关键词 酪蛋白 酶解 抗氧化多肽 自由基清除
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EGCG体外抗氧化性能及其对酪蛋白乳化性能的影响 被引量:4
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作者 董新玲 《陕西科技大学学报》 CAS 2018年第5期57-62,76,共7页
表没食子儿茶素没食子酸酯(EGCG)是绿茶中含量最高的一种抗氧化物质.本实验采用六种方法对EGCG的抗氧化活性进行了测定和评价,并研究了不同浓度未氧化EGCG及氧化EGCG对酪蛋白乳化性能的影响.实验结果表明:EGCG具有优良的抗氧化能力,这... 表没食子儿茶素没食子酸酯(EGCG)是绿茶中含量最高的一种抗氧化物质.本实验采用六种方法对EGCG的抗氧化活性进行了测定和评价,并研究了不同浓度未氧化EGCG及氧化EGCG对酪蛋白乳化性能的影响.实验结果表明:EGCG具有优良的抗氧化能力,这主要源于其高效的(ABTS、DPPH和OH)自由基清除能力,及一定的金属离子(Fe2+和Cu2+)鳌合能力.EGCG及其氧化物的添加抑制了酪蛋白的乳化活性,但提高了其乳化稳定性.该研究对于EGCG等天然酚类物质在富含蛋白食品生产加工中的应用提供了一定的依据和借鉴. 展开更多
关键词 EGCG 抗氧化 自由基 酪蛋白 乳化活性 乳化稳定性
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氧化酪蛋白对小鼠组织抗氧化能力及血液肽组成的影响 被引量:5
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作者 吴伶艳 李竹青 +2 位作者 吴秋萍 乐国伟 施用晖 《世界科技研究与发展》 CSCD 2011年第3期489-493,共5页
目的研究酪蛋白经过加热氧化和脂质过氧化物MDA氧化后,对小鼠体内氧化还原状态及小鼠血液中肽组成的影响。方法酪蛋白经100℃加热(0、30、60、90 min)和丙二醛(MDA)氧化(MDA终浓度0、0.2、20、200 mmol/L)处理,测定其羰基、巯基含量及... 目的研究酪蛋白经过加热氧化和脂质过氧化物MDA氧化后,对小鼠体内氧化还原状态及小鼠血液中肽组成的影响。方法酪蛋白经100℃加热(0、30、60、90 min)和丙二醛(MDA)氧化(MDA终浓度0、0.2、20、200 mmol/L)处理,测定其羰基、巯基含量及表面疏水性的变化。小鼠分为四组,分别灌胃生理盐水、正常酪蛋白、加热90 min、MDA氧化酪蛋白,测定0、30、60、90、120、160min血液中自由基水平,并采用HPLC-MS分析灌胃后120 min小鼠血浆肽组分的变化,测定小鼠的肝脏、空肠、十二指肠组织抗氧化能力指标(总抗氧化能力T-AOC、丙二醛MDA、还原型谷胱甘肽GSH)。结果酪蛋白经过两种氧化处理,羰基含量均随氧化程度呈显著上升,巯基含量呈下降趋势。加热氧化后酪蛋白表面疏水性比正常酪蛋白低,而MDA氧化导致疏水性上升。小鼠血液中自由基最高峰出现在灌胃后160min。灌胃两种方式处理的酪蛋白,血液中自由基的含量均显著高于灌胃正常酪蛋白组(P<0.05),肝脏、空肠、十二指肠还原型谷胱甘肽(GSH)含量和总抗氧化能力(T-AOC)均低于正常酪蛋白组,丙二醛(MDA)含量显著提高(P<0.05)。HPLC-MS分析显示,灌胃MDA氧化酪蛋白组血液中肽组成与对照组不同,出现C_7H_(15)N_4O含羰基类的物质。结论氧化会导致酪蛋白理化性质的改变,摄入氧化酪蛋白引起机体的氧化应激,降低组织抗氧化能力。 展开更多
关键词 酪蛋白氧化 羰基 自由基 抗氧化能力 血液肽HPLC—MS
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Are therapeutic diets an emerging additional choice in autism spectrum disorder management?
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作者 M.Gogou G.Kolios 《World Journal of Pediatrics》 SCIE CAS CSCD 2018年第3期215-223,共9页
Background A nutritional background has been recognized in the pathophysiology of autism and a series of nutritional interventions have been considered as complementary therapeutic options. As available treatments and... Background A nutritional background has been recognized in the pathophysiology of autism and a series of nutritional interventions have been considered as complementary therapeutic options. As available treatments and interventions are not effective in all individuals, new therapies could broaden management options for these patients. Our aim is to provide current literature data about the effect of therapeutic diets on autism spectrum disorder. Data sources A systematic review was conducted by two reviewers independently. Prospective clinical and preclinical stud-ies were considered. Results Therapeutic diets that have been used in children with autism include ketogenic and gluten/casein-free diet. We were able to identify 8 studies conducted in animal models of autism demonstrating a beneficial effect on neurophysiological and clinical parameters. Only 1 clinical study was found showing improvement in childhood autism rating scale after implemen-tation of ketogenic diet. With regard to gluten/casein-free diet, 4 clinical studies were totally found with 2 of them showing a favorable outcome in children with autism. Furthermore, a combination of gluten-free and modified ketogenic diet in a study had a positive effect on social affect scores. No serious adverse events have been reported. Conclusions Despite encouraging laboratory data, there is controversy about the real clinical effect of therapeutic diets in patients with autism. More research is needed to provide sounder scientific evidence. 展开更多
关键词 AUTISM Children Gluten/casein-free DIET KETOGENIC DIET THERAPEUTIC DIET
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Inhibition of CK2 Activity by TCDD via Binding to ATP-competitive Binding Site of Catalytic Subunit: Insight from Computational Studies 被引量:1
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作者 XU Xian-jin CANNISTRARO Salvatore +3 位作者 BIZZARRI Anna-rita ZENG Yi CHEN Wei-zu WANG Cun-xin 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2013年第2期299-306,共8页
Alternative mechanisms of toxic effects induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD), instead of the binding to aryl hydrocarbon receptor(AhR), have been taken into consideration. It has been recently show... Alternative mechanisms of toxic effects induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD), instead of the binding to aryl hydrocarbon receptor(AhR), have been taken into consideration. It has been recently shown that TCDD reduces rapidly the activity of CK2(casein kinase II) both in vivo and in vitro. It is found that TCDD has high molecular similarities to the known inhibitors of CK2 catalytic subunit(CK2a). This suggests that TCDD could also be an ATP-competitive inhibitor of CK2a. In this work, docking TCDD to CK2 was carried out based on the two structures of CK2a from maize and human, respectively. The binding free energies of the predicted CK2a-TCDD complexes estimated by the molecular mechanics/Poisson-Boltzmann surface area(MM/PBSA) method are from -85.1 kJ/mol to -114.3 kJ/mol for maize and are from -96.1 kJ/mol to -118.2 kJ/mol for human, which are comparable to those estimated for the known inhibitor and also ATP with CK2a. The energetic analysis also reveals that the van der Waals interaction is the dominant contribution to the binding free energy. These results are also useful for designing new drugs for a target of overexpressing CK2 in cancers. 展开更多
关键词 Casein kinase II DIOXIN Inhibitor Modeling Binding free energy
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