Objective:To explore the antifungal and antibacterial activity of methanolic extract of Cassiaalata(C.alata)and its formulations for skin diseases.Methods:Sundried leaves of C.alata Linn.were extracted using different...Objective:To explore the antifungal and antibacterial activity of methanolic extract of Cassiaalata(C.alata)and its formulations for skin diseases.Methods:Sundried leaves of C.alata Linn.were extracted using different solvents as follows:water,methanol,ethanol,n-hexane and lastly with acetone.The crude extract was investigated for antifungal and antibacterial activities using disc diffusion method against Coccidioidesimmitis,Exophilia dermatitidis,Aspergillus fumigatus and human pathogenic fungi Candida albicans and a group of bacteria,Staphylococcus aureus.The minimum inhibitory concentrations of the methanolic extract were determined using the agar dilution method.Herbal ointments were prepared by incorporating the methanol extract of C.alata into emulsifying ointment to obtain different concentrations of 25,50,100,and 200 mg/mL.Results:The methanol extraction gave the maximum extraction.The formulated C.alata ointment when compared with standard drugs nystatin and streptomycin in vitro was more effective against the microorganisms.Conclusions:This study showed that C.alata had antifungal and antibacterial activities when formulated as ointment for topical use and could,therefore,explained its folkloric use for the treatment of dermatitis.展开更多
Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were...Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were then subjected to an in vitro test for their effectiveness on mycelia growth inhibition against some important plant pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides, Fusarium oxysporum fsp. lycopersici, Sclerotium rolfsii, Phytophthora infestans and Pythium sp. in comparison to commercial fungicides such as copper oxychloride and mancozeb. Reduction of the fungal growth was significantly obtained with C. longa extracts and the best median effective inhibitory concentration (IC50) value of 6.07, 6.50 and 7.13 mg/ml was from the ethanol extract for S. rolfsii, C. gloeosporioides and F. oxysporum fsp. lycopersici respectively. While all extracts from C. alata were almost the least effective against these fungi. The efficacy of C. longa extracts therefore, provided an alternative regime for the control of the fungal diseases and a promising appreciable choice for a replacement of chemical fungicides.展开更多
文摘Objective:To explore the antifungal and antibacterial activity of methanolic extract of Cassiaalata(C.alata)and its formulations for skin diseases.Methods:Sundried leaves of C.alata Linn.were extracted using different solvents as follows:water,methanol,ethanol,n-hexane and lastly with acetone.The crude extract was investigated for antifungal and antibacterial activities using disc diffusion method against Coccidioidesimmitis,Exophilia dermatitidis,Aspergillus fumigatus and human pathogenic fungi Candida albicans and a group of bacteria,Staphylococcus aureus.The minimum inhibitory concentrations of the methanolic extract were determined using the agar dilution method.Herbal ointments were prepared by incorporating the methanol extract of C.alata into emulsifying ointment to obtain different concentrations of 25,50,100,and 200 mg/mL.Results:The methanol extraction gave the maximum extraction.The formulated C.alata ointment when compared with standard drugs nystatin and streptomycin in vitro was more effective against the microorganisms.Conclusions:This study showed that C.alata had antifungal and antibacterial activities when formulated as ointment for topical use and could,therefore,explained its folkloric use for the treatment of dermatitis.
文摘Crude plant extracts of ringworm cassia, Cassia alata L. and turmeric, Curcuma longa L. were prepared by either hot water or organic solvents such as ethanol and ether. Various concentrations of the crude extract were then subjected to an in vitro test for their effectiveness on mycelia growth inhibition against some important plant pathogenic fungi such as Alternaria alternata, Colletotrichum gloeosporioides, Fusarium oxysporum fsp. lycopersici, Sclerotium rolfsii, Phytophthora infestans and Pythium sp. in comparison to commercial fungicides such as copper oxychloride and mancozeb. Reduction of the fungal growth was significantly obtained with C. longa extracts and the best median effective inhibitory concentration (IC50) value of 6.07, 6.50 and 7.13 mg/ml was from the ethanol extract for S. rolfsii, C. gloeosporioides and F. oxysporum fsp. lycopersici respectively. While all extracts from C. alata were almost the least effective against these fungi. The efficacy of C. longa extracts therefore, provided an alternative regime for the control of the fungal diseases and a promising appreciable choice for a replacement of chemical fungicides.
