Objetive: To explore the potential insecticidal, ovipositor deterrent and antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Metho...Objetive: To explore the potential insecticidal, ovipositor deterrent and antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Method: The activities were evaluated using standard protocols.In these bioassays, the cowpea seeds were used directly as an insect feed. The activity of the extract and isolated compounds were tested at concentrations of 100, 200 and 300 μg/mL and compared to neem oil and cinnamaldehyde(as standard positive controls). Phytochemical analysis of the ethyl acetate extract was done through a number of chromatographic techniques and the structures of the isolated compounds were established through comprehensive spectroscopic analysis including 2 D-NMR and ESI-MS studies.Results: Fractionation of the active ethyl acetate extract resulted in the isolation of one known anthraquinone,aurantio-obtusin(1) and a novel compound that was named as cassiatorin(2). Compounds1 and 2 showed comparable insect antifeedant properties with the positive controls while their insecticidal and ovipositor deterrent effects were far superior to the standard controls.Conclusions: It is thus concluded that Senna tora extracts and the isolated compounds(1 and2) may be employed in the postharvest management of stored cowpea seeds and as other crop protectants.展开更多
Cassia tora Linn.More commonly known as‘Charota’in hindi language in India,is a rainwater wasteland herb.For years,the leaves and seeds of Cassia tora have been used in the Ayurveda,Traditional Chinese Medicine(TCM)...Cassia tora Linn.More commonly known as‘Charota’in hindi language in India,is a rainwater wasteland herb.For years,the leaves and seeds of Cassia tora have been used in the Ayurveda,Traditional Chinese Medicine(TCM),and folk medicine of tropics for the various medicinal properties it possesses.By reviewing various literature on the plant,it was found that it is great for health as it possesses several protective properties like:h epatoprotective,ne phroprotective,c ataract protective,s kin protective,and n europrotective properties.Apart from these properties like,v asodilatory,a nti-tumor,a nti-oxidant,l axative,a nti-d iabetic,a nti-i nflammatory,a nti-e strogenic,are also significant medicinal activities that can be further used to develop neutraceuticals for diseases like cancer and diabetes.Lastly,the compounds found in Cassia Tora are also efficient anti-bacterial,anti-fungal and anti-helminthic agents.Some of its medicinal properties have been reported only in ethnomedicine,while some tested in model systems,and some verified through clinical trials.We evaluate the current state of literature on these medicinal activities.We have found this leguminous herb to have potentially multiple characteristics that might help in mitigating current pandemic with literature evidence of anti SARS-CoV activity.Further,an in-silico analysis of the molecule Aurantio-Obtusin(AO)showed efficient binding to various SARS-CoV2 proteases and host factor molecules involved in inflammatory pathways.However,more experimental and clinical studies with single isolated and purified natural compounds are warranted to confirm such useful activities of the wasteland herb,for it’s health food,medical and economic utilization.展开更多
[Objectives]To understand the content changes of fatty acid compositions and seedling growths during seed germinations of Cassia obtusifolia L.[Methods]The fatty acid compositions of germinations and seedling growths ...[Objectives]To understand the content changes of fatty acid compositions and seedling growths during seed germinations of Cassia obtusifolia L.[Methods]The fatty acid compositions of germinations and seedling growths of C.obtusifolia seeds treated with different concentrations of gibberellin(GA3)and naphthalene acetic acid(NAA)were analyzed using gas chromatograph-mass spectrometer(GC-MS),and the germination rates,root lengths,plant heights and fresh weights of their seedlings were analyzed.[Results]The contents of eicosenoic acid,α-linoleic acid,arachidic acid,eicosadienoic acid and eicosapentaenoic acid of C.obtusifolia seeds treated with different concentrations of GA3 and NAA decreased with germination time.There were significant differences between the ratios of germinations of C.obtusifolia seeds treated with different concentrations of GA3 and NAA.GA3 and NAA of different ratio treatments could promote seedling heights and fresh weights of C.obtusifolia,but increases of seedling heights and fresh weights were different.Eicosenoic acid,α-linoleic acid,arachidic acid,eicosadienoic acid and eicosapentaenoic acid were remarkably negative correlation with seedling heights and fresh weights.[Conclusions]The fatty acid components of C.obtusifolia seed germination and growth of their seedlings were affected.It indicated that GA3 and NAA treatments can affect fatty acid metabolism,the germination rates,root lengths,plant heights and fresh weights of C.obtusifolia.It is intended to provide a theoretical basis and technological reference for standardized cultivated techniques of C.obtusifolia.展开更多
基金The financial assistance from the Swedish International Development Agency for some of the study (travel grant for Valantine Mbatchou)
文摘Objetive: To explore the potential insecticidal, ovipositor deterrent and antifeedant effects of ethyl acetate extract of the seeds of Senna tora(Syn. Cassia tora) against cowpea weevil(Callosobruchus maculatus).Method: The activities were evaluated using standard protocols.In these bioassays, the cowpea seeds were used directly as an insect feed. The activity of the extract and isolated compounds were tested at concentrations of 100, 200 and 300 μg/mL and compared to neem oil and cinnamaldehyde(as standard positive controls). Phytochemical analysis of the ethyl acetate extract was done through a number of chromatographic techniques and the structures of the isolated compounds were established through comprehensive spectroscopic analysis including 2 D-NMR and ESI-MS studies.Results: Fractionation of the active ethyl acetate extract resulted in the isolation of one known anthraquinone,aurantio-obtusin(1) and a novel compound that was named as cassiatorin(2). Compounds1 and 2 showed comparable insect antifeedant properties with the positive controls while their insecticidal and ovipositor deterrent effects were far superior to the standard controls.Conclusions: It is thus concluded that Senna tora extracts and the isolated compounds(1 and2) may be employed in the postharvest management of stored cowpea seeds and as other crop protectants.
文摘Cassia tora Linn.More commonly known as‘Charota’in hindi language in India,is a rainwater wasteland herb.For years,the leaves and seeds of Cassia tora have been used in the Ayurveda,Traditional Chinese Medicine(TCM),and folk medicine of tropics for the various medicinal properties it possesses.By reviewing various literature on the plant,it was found that it is great for health as it possesses several protective properties like:h epatoprotective,ne phroprotective,c ataract protective,s kin protective,and n europrotective properties.Apart from these properties like,v asodilatory,a nti-tumor,a nti-oxidant,l axative,a nti-d iabetic,a nti-i nflammatory,a nti-e strogenic,are also significant medicinal activities that can be further used to develop neutraceuticals for diseases like cancer and diabetes.Lastly,the compounds found in Cassia Tora are also efficient anti-bacterial,anti-fungal and anti-helminthic agents.Some of its medicinal properties have been reported only in ethnomedicine,while some tested in model systems,and some verified through clinical trials.We evaluate the current state of literature on these medicinal activities.We have found this leguminous herb to have potentially multiple characteristics that might help in mitigating current pandemic with literature evidence of anti SARS-CoV activity.Further,an in-silico analysis of the molecule Aurantio-Obtusin(AO)showed efficient binding to various SARS-CoV2 proteases and host factor molecules involved in inflammatory pathways.However,more experimental and clinical studies with single isolated and purified natural compounds are warranted to confirm such useful activities of the wasteland herb,for it’s health food,medical and economic utilization.
基金Supported by the National Natural Science Foundation of China(41761010&81260641)Science and Technology Foundation of Guizhou province of China([2017]1121)the Project of Scientific Research of Doctors of Guizhou Normal University.
文摘[Objectives]To understand the content changes of fatty acid compositions and seedling growths during seed germinations of Cassia obtusifolia L.[Methods]The fatty acid compositions of germinations and seedling growths of C.obtusifolia seeds treated with different concentrations of gibberellin(GA3)and naphthalene acetic acid(NAA)were analyzed using gas chromatograph-mass spectrometer(GC-MS),and the germination rates,root lengths,plant heights and fresh weights of their seedlings were analyzed.[Results]The contents of eicosenoic acid,α-linoleic acid,arachidic acid,eicosadienoic acid and eicosapentaenoic acid of C.obtusifolia seeds treated with different concentrations of GA3 and NAA decreased with germination time.There were significant differences between the ratios of germinations of C.obtusifolia seeds treated with different concentrations of GA3 and NAA.GA3 and NAA of different ratio treatments could promote seedling heights and fresh weights of C.obtusifolia,but increases of seedling heights and fresh weights were different.Eicosenoic acid,α-linoleic acid,arachidic acid,eicosadienoic acid and eicosapentaenoic acid were remarkably negative correlation with seedling heights and fresh weights.[Conclusions]The fatty acid components of C.obtusifolia seed germination and growth of their seedlings were affected.It indicated that GA3 and NAA treatments can affect fatty acid metabolism,the germination rates,root lengths,plant heights and fresh weights of C.obtusifolia.It is intended to provide a theoretical basis and technological reference for standardized cultivated techniques of C.obtusifolia.
基金National Science and Technology Support Program(2006BA106A15)Special funds for basic research and operating expenses of the Ministry of Education(2009-3-7/2510JS052)
