Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castampsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty-five loci were identified with ...Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castampsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty-five loci were identified with 41 oligonucleotide primers, out of which 157 loci were polymorphic and accounted for 40.78% of total genetic diversity at species level. Shannon's indices of diversity (I) and Nei's gene diversity ( h) were 0.459 7 and 0.296 at the species level, respectively. The result showed that genetic variation of C. fargesii populations mainly existed within populations. Genetic differentiation (Hsp-hpop)/Hsp estimated with Shannon's index of diversity and coefficient of gene differentiation (Gst) were 0.047 6 and 0.042 9 respectively, which were confirmed by the analysis of molecular variance (AMOVA). Therefore, it is apparent that within-population variation accounted for 94.97% and among-populations variation accounted for only 5.03% of the total genetic diversity. AMOVA also indicated that there was significant differentiation among populations as well as among individuals within a population.展开更多
Castanopsis fargesii is a good afforestation plants and various microorganisms play important roles in mediating the growth and ecological functions of this species.In this study,we evaluated changes in microbial comm...Castanopsis fargesii is a good afforestation plants and various microorganisms play important roles in mediating the growth and ecological functions of this species.In this study,we evaluated changes in microbial communities in soil samples from C.fargesii forests.The phospholipid fatty acid(PLFA)biomarker method was used to obtain bacteria,fungi,actinomycetes,gram-positive bacteria(G?),gram-negative bacteria(G-),aerobic bacteria,and anaerobic bacteria to investigate spatiotemporal changes in microbial communities during the growing season.The results show that soil microorganisms were mainly concentrated in the upper 20-cm layer,demonstrating an obvious surface aggregation(P<0.05).Large amounts of litter and heavy rainfall during the early growing season resulted in the highest PLFA contents for various microorganisms,whereas relatively low and stable levels were observed during other times.The dominant species during each period were bacteria.G+ or aerobic bacteria were the main bacterial populations,providing insights into the overall trends of soil bacterial PLFA contents.Due to the relative accumulation of refractory substances during the later stages of litter decomposition,the effects of fungi increased significantly.Overall,our findings demonstrate that the main factors influencing microbial communities were litter,rainfall,and soil field capacity.展开更多
文摘Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castampsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty-five loci were identified with 41 oligonucleotide primers, out of which 157 loci were polymorphic and accounted for 40.78% of total genetic diversity at species level. Shannon's indices of diversity (I) and Nei's gene diversity ( h) were 0.459 7 and 0.296 at the species level, respectively. The result showed that genetic variation of C. fargesii populations mainly existed within populations. Genetic differentiation (Hsp-hpop)/Hsp estimated with Shannon's index of diversity and coefficient of gene differentiation (Gst) were 0.047 6 and 0.042 9 respectively, which were confirmed by the analysis of molecular variance (AMOVA). Therefore, it is apparent that within-population variation accounted for 94.97% and among-populations variation accounted for only 5.03% of the total genetic diversity. AMOVA also indicated that there was significant differentiation among populations as well as among individuals within a population.
基金This study was supported by the Science and Technology Basic Work Special(No 2014FY120700).
文摘Castanopsis fargesii is a good afforestation plants and various microorganisms play important roles in mediating the growth and ecological functions of this species.In this study,we evaluated changes in microbial communities in soil samples from C.fargesii forests.The phospholipid fatty acid(PLFA)biomarker method was used to obtain bacteria,fungi,actinomycetes,gram-positive bacteria(G?),gram-negative bacteria(G-),aerobic bacteria,and anaerobic bacteria to investigate spatiotemporal changes in microbial communities during the growing season.The results show that soil microorganisms were mainly concentrated in the upper 20-cm layer,demonstrating an obvious surface aggregation(P<0.05).Large amounts of litter and heavy rainfall during the early growing season resulted in the highest PLFA contents for various microorganisms,whereas relatively low and stable levels were observed during other times.The dominant species during each period were bacteria.G+ or aerobic bacteria were the main bacterial populations,providing insights into the overall trends of soil bacterial PLFA contents.Due to the relative accumulation of refractory substances during the later stages of litter decomposition,the effects of fungi increased significantly.Overall,our findings demonstrate that the main factors influencing microbial communities were litter,rainfall,and soil field capacity.
