The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S<sub>180</sub> tumor cells were studied throughchemiluminescence m...The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S<sub>180</sub> tumor cells were studied throughchemiluminescence measurement and[<sup>3</sup>H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[<sup>3</sup>H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O<sub>2</sub><sup> </sup>and H<sub>2</sub>O<sub>2</sub> in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.展开更多
基金Supported by the National Natural Science Foundation of China No.3860835
文摘The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S<sub>180</sub> tumor cells were studied throughchemiluminescence measurement and[<sup>3</sup>H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[<sup>3</sup>H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O<sub>2</sub><sup> </sup>and H<sub>2</sub>O<sub>2</sub> in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.
