A model for predicting retention time of solutes under linear gradient elution conditions has been established. In this model, the theoretical expressions under different elution modes were derived and tested with the...A model for predicting retention time of solutes under linear gradient elution conditions has been established. In this model, the theoretical expressions under different elution modes were derived and tested with the retention behaviors of p hydroxybenzaldehyde, vanillin, biphenyl, phenanthrene in gradient elution. With taking into account the dwell time of the instrumental system, the theoretically predicted retention times agree well with those experimentally determined.展开更多
Based on the inhibition by hydroquinone, catechol and resorcinol of chemiluminescence from luminol potassium hexacyanoferrate(Ⅲ) system, a high performance liquid chromatographic procedure with chemiluminescent detec...Based on the inhibition by hydroquinone, catechol and resorcinol of chemiluminescence from luminol potassium hexacyanoferrate(Ⅲ) system, a high performance liquid chromatographic procedure with chemiluminescent detection was developed for simultaneous determination of hydroquinone, catechol and resorcinol. The separation was carried out on RP C 18 column at 37 ℃ by using a step gradient elution made by the mobile phases of methanol(A) and 0 05% H 3PO 4(B). The gradient elution condition was 95% B, 5 min; 95%B 50%B, 1 min; 50% B, 6 min. This method has the merits of high senstivity and good selectivity. The detection limits for hydroquinone, catechol and resorcinol are 2 0×10 -9 , 3 2×10 -9 and 6 8×10 -7 g/mL, respectively. The relative standard deviation( n =5) is 1 9% for 2 5×10 -7 g/mL hydroquinone, 1 0% for 7 5×10 -7 g/mL catechol, 4 0% for 5 0×10 -5 g/mL resorcinol, respectively. The recovery is in the range of 94%—104%. [WT5HZ]展开更多
基金theNationalNaturalScienceFoundationofChina (No .2 0 0 75 0 0 5 ) theGrantfromMinistryofScienceandTechnologytheNaturalScienceFoundationofHebeiProvince (No .2 0 2 0 96)
文摘A model for predicting retention time of solutes under linear gradient elution conditions has been established. In this model, the theoretical expressions under different elution modes were derived and tested with the retention behaviors of p hydroxybenzaldehyde, vanillin, biphenyl, phenanthrene in gradient elution. With taking into account the dwell time of the instrumental system, the theoretically predicted retention times agree well with those experimentally determined.
文摘Based on the inhibition by hydroquinone, catechol and resorcinol of chemiluminescence from luminol potassium hexacyanoferrate(Ⅲ) system, a high performance liquid chromatographic procedure with chemiluminescent detection was developed for simultaneous determination of hydroquinone, catechol and resorcinol. The separation was carried out on RP C 18 column at 37 ℃ by using a step gradient elution made by the mobile phases of methanol(A) and 0 05% H 3PO 4(B). The gradient elution condition was 95% B, 5 min; 95%B 50%B, 1 min; 50% B, 6 min. This method has the merits of high senstivity and good selectivity. The detection limits for hydroquinone, catechol and resorcinol are 2 0×10 -9 , 3 2×10 -9 and 6 8×10 -7 g/mL, respectively. The relative standard deviation( n =5) is 1 9% for 2 5×10 -7 g/mL hydroquinone, 1 0% for 7 5×10 -7 g/mL catechol, 4 0% for 5 0×10 -5 g/mL resorcinol, respectively. The recovery is in the range of 94%—104%. [WT5HZ]
