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Effects of Headgroups and Serum on Gene Transfection of Alkaline Amino Acid Based Cationic Lipids
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作者 LI Li YANG Yang NIE Yu HE Bin GU Zhong-wei 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2009年第1期69-71,共3页
Three cationic lipids with lysylated(1), histidylated(2), and arginylated(3) headgroups and cholesterol hydrophobic moiety were synthesized. The average sizes of liposomes and lipoplexes were around 100 and 160 ... Three cationic lipids with lysylated(1), histidylated(2), and arginylated(3) headgroups and cholesterol hydrophobic moiety were synthesized. The average sizes of liposomes and lipoplexes were around 100 and 160 nm, respectively. The gene transfection efficiency of the three lipoplexes loaded with pGL3 or pORF-LacZ was compared on 293T cells in the presence or the absence of serum. The transfection efficiency of the three lipoplexes in a serum-free medium was 2 to 3-fold higher than that of dioleoyl-trimethylammonium propane(DOTAP). In the presence of serum, however, most of the lipoplexes showed lower transfection activities; only lipoplex 3 retained its high transfection efficiency. 展开更多
关键词 cationic lipids Alkaline amino acids SERUM Gene delivery Transfection efficiency
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Ionic liquid-based in situ dynamically self-assembled cationic lipid nanocomplexes(CLNs)for enhanced intranasal siRNA delivery
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作者 Luyu Zhang Zirong Dong +7 位作者 Shuai Yu Guangyue Li Weiwen Kong Wenjuan Liu Haisheng He Yi Lu Wei Wu Jianping Qi 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第7期367-371,共5页
This research aims to develop a non-invasive strategy for small interfering RNA(siRNA)nasal delivery based on ionic liquids(ILs)and cationic lipid(2,3-dioleoyloxy-propyl)-trimethylammonium-chloride(DOTAP).Other than t... This research aims to develop a non-invasive strategy for small interfering RNA(siRNA)nasal delivery based on ionic liquids(ILs)and cationic lipid(2,3-dioleoyloxy-propyl)-trimethylammonium-chloride(DOTAP).Other than the classical role of penetration enhancer,ILs also acted as superior solvents to simultaneously load siRNA and DOTAP,forming siRNA-DOTAP-ILs(siRNA-DILs)formulations.During nasal mucosa penetration,DOTAP and ILs components self-assembled into cationic lipid nanocomplexes to load siRNA for enhanced in situ transfection.The siRNA-DILs demonstrated resistance against RNase,significant mucosa penetration,prolonged nasal retention,and satisfying gene-silencing efficacy at lower dosage.Meanwhile,DILs were also able to deliver KCa3.1-targeted siRNA effectively for the treatment of allergic rhinitis in rat model by nasal route.Thus,DILs have great potentials to deliver biological macromolecules across nasal mucosa by in situ dynamic self-assembly. 展开更多
关键词 Ionic liquids cationic lipids lipid nanoparticles siRNA Allergic rhinitis
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Application of cationic liposomes for delivery of nucleic acids 被引量:5
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作者 Gayong Shim Mi-Gyeong Kim +1 位作者 Joo Yeon Park Yu-Kyoung Oh 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2013年第2期72-80,共9页
Nucleic acid-based bioactive substances have recently emerged as a new class of nextgeneration therapeutics, but their development has been limited by their relatively weakdelivery into target cells. Cationic liposome... Nucleic acid-based bioactive substances have recently emerged as a new class of nextgeneration therapeutics, but their development has been limited by their relatively weakdelivery into target cells. Cationic liposomes have been studied as a means to enhance thestability of nucleic acid therapeutics in the bloodstream and improve their cellular delivery.As nucleic acid therapeutics, siRNA and plasmid DNA have been extensively tested fordelivery using cationic liposomes. This review discusses recent progress in the applicationof cationic liposomes for the delivery of nucleic acid therapeutics. 展开更多
关键词 cationic lipid Liposomes Delivery Nucleic acid therapeutics SIRNA Plasmid DNA
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Optimization on cationic liposome-mediated cell transfection of plasmid DNA 被引量:1
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作者 Mingang Ying Changhua Zhuo Weidong Zang 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第5期290-292,共3页
Objective:The development of gene carriers for efficient gene delivery into cells has attracted growing attention in recent years.The aim of this study was to achieve a better outcome of AAV-293 cells transfection by ... Objective:The development of gene carriers for efficient gene delivery into cells has attracted growing attention in recent years.The aim of this study was to achieve a better outcome of AAV-293 cells transfection by plasmid DNA.Methods:We studied the optimal condition for higher efficiency of cationic lipid-mediated cell transfection.Four experimental groups were set.Plasmid DNA and liposome were mixed in each groups at different ratios(μg:μL),1:2.5,1:3.5,1:4.0 and 1:5.0,respectively.LacZ gene functioned as reporter gene,measuring the transfection efficiency of the four groups using the method of X-gal staining.Results:When the ratio was 1:3.5,the cell transfection rate was the highest.While the ratio of 1:2.5 recommended by product manual achieve the lowest transfection rate.Their difference had statistical significance.Conclusion:In order to obtain a higher transfection efficiency,optimization on conditions of the ratio of plasmid DNA to liposome is necessary in cell transfection. 展开更多
关键词 cell transfection cationic lipid plasmid DNA cell culture transfection efficiency
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Transfection of 3′,3′′-bis-peptide-siRNA conjugate by cationic lipoplexes mixed with a neutral cytosin-1-yl-lipid
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作者 杨梦依 孙晶 +3 位作者 王超 张艳芬 张礼和 杨振军 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2017年第10期719-726,共8页
Cationic lipids have been applied to siRNA delivery for tumor therapeutics. However, the excess positive charges of these nanoplexes may lead to high cytotoxicity and nonnegligible immunogenicity both in vitro and in ... Cationic lipids have been applied to siRNA delivery for tumor therapeutics. However, the excess positive charges of these nanoplexes may lead to high cytotoxicity and nonnegligible immunogenicity both in vitro and in vivo, which limited the applications of gene drugs. We constructed multi-component lipoplex to delivery 3',3"-bis-peptide-siRNA conjugate (pp-siRNA) by the treatment of melanoma. Based on the previous studies that the gemini lipid (CLD) encapsulated pp-siRNA, a novel neutral cytosin-l-yl- lipid (DNCA) was considered to replace a certain ration of CLD by hydrogen bonds and ~t-n stacking for reducing the cytotoxicity. It similarly retained in both the loading efficiency and targeted mRNA inhibition when DNCA was accounted for 40% in the lipoplex, with lower toxicity. Moreover, CLD/DNCA/pp-siRNA nanoplex could be uptake in A375 cells and internalized mainly by macropinocytosis and caveolin-mediated endocytosis. Besides, 90% CLD/DNCA/pp-siRNA nanoplexes presented the highest efficient knockdown for the mutant B-RAF mRNA (-80%). All the results demonstrated that the mixed cationic and neutral lipids could efficiently realize the delivery of pp-siRNA and had potential application for cancer therapy. 展开更多
关键词 3' 3"-Bis-peptide siRNA conjugate Gemini-like cationic lipid Cytosin-1-yl-lipid Melanoma therapy
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