Objective:To evaluate the antibacterial and antioxidant activities and to identify the volatile bioactive compounds present in different crude extracts of the seaweed Caulerpa racemosa var.cylindracea.Methods:Caulerpa...Objective:To evaluate the antibacterial and antioxidant activities and to identify the volatile bioactive compounds present in different crude extracts of the seaweed Caulerpa racemosa var.cylindracea.Methods:Caulerpa racemosa harvested from the intertidal zone of Mostaganem coast(N 35?54’37.94",E 0?3’17.37")was subjected to Soxhlet extraction using methanol,chloroform,and hexane solvents.Antioxidant properties were assessed by using 2,2’-diphenyl-1-picrylhydrazyl(DPPH),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)andβ-carotene bleaching assays.The antibacterial activity was evaluated on six standard bacterial strains using the agar disc diffusion method.The GC-MS analysis was performed using non-polar and polar capillary columns.Results:The chloroform extract of Caulerpa racemosa exhibited higher contents of polyphenols[(123.91±1.46)mg gallic acid equivalent/g dry extract]and tannins[(59.28±5.43)mg catechin equivalent/g dry extract](P<0.001)and was the most effective in scavenging DPPH[(1.98±0.08)mg/m L]and ABTS[(1.66±0.05)mg/m L]radicals.The hexane extract displayed the best antibacterial activity against Staphylococcus aureus,Bacillus cereus,and Pseudomonas aeruginosa,producing inhibition zones of(11.16±0.76),(9.00±0.00)and(9.33±1.15)mm,respectively.The l-(+)-ascorbic acid 2,6-dihexadecanoate and 4-hydroxy-2 methylproline were among the most abundant volatile compounds.Besides conventional fatty acids,cis-10-heptadecenoic acid,nonahexacontanoic acid,and dodecanoic acid,3-hydroxy-were identified.Two phytosterols were identified:stigmast-5-en-3-ol-(12.9%)and stigmast-5-en-3.beta.-ol,(24 S)-(4.57%).Conclusions:The preliminary identification of the volatile compounds reveals the presence of some new bioactive components not reported previously in Caulerpa racemosa from other geographical areas.Some of these compounds possess an interesting potential for pharmaceutical/nutraceutical applications.展开更多
A polysaccharide, CrvpPS, was isolated from Caulerpa racemosa var peltata. It was reacted with nano-selenium in distilled water containing ascorbic acid (Vit C) to form a stable CrvpPS-nano-Se complex. The immunomodul...A polysaccharide, CrvpPS, was isolated from Caulerpa racemosa var peltata. It was reacted with nano-selenium in distilled water containing ascorbic acid (Vit C) to form a stable CrvpPS-nano-Se complex. The immunomodulatory effects of CrvpPS and CrvpPS-nano-Se on T lymphocytes subgroups and NK cells in mice were investigated. After intragastric administration for 10 days separately, both CrvpPS and CrvpPS-nano-Se showed significant stimulatory functions to thymus gland of mice. Moreover, the CrvpPS-nano-Se induced the percentage of CD3+, CD3+CD4+, NK cells and the CD4+/CD8+ value to increase significantly (P<0.05) when analyzed by flow cytometry, which is better than the CrvpPS, sucrose-nano-Se, and even the positive drug levamisole.展开更多
目的分离鉴定中国南海总状蕨藻（Cauler pa rucennosu）中的脂肪酸类化学成分。供药理活性筛选。方法综合利用各种柱层析方法分离纯化化合物并利用光谱学方法解析化合物结构；采用M3-F法对所有化合物进行神经细胞损伤保护作用评价；采...目的分离鉴定中国南海总状蕨藻（Cauler pa rucennosu）中的脂肪酸类化学成分。供药理活性筛选。方法综合利用各种柱层析方法分离纯化化合物并利用光谱学方法解析化合物结构；采用M3-F法对所有化合物进行神经细胞损伤保护作用评价；采用酶标法对所有化合物进行蛋白酪氨酸磷酸酯酶抑制活性评价。结果从中分离得到12个脂肪酸及衍生物，分别鉴定为棕榈酸（1）、硬脂酸、（2）、十八皖二酯二乙酯、（3）、正二十三烷酸甘油酯（4）、（8E）-heplade0c-8-em7-one（5）、（8Z，11Z）-heptadeca-8，11-dienoicacid（6）、ethyl linolenoate（7）、（4Z，7Z．10Z，13Z）-ethyl hexadeca-4，7，10，13-telraenoate（8）、β-dimorphecolic acid（9）、α-adimorphecolic acid（10）、（9R，10E，12Z）-9-hydroxyoctadeca-10。12-dienoicacid（11）、（9R，10E）-9-hvdroxyoctadec-10-enoicacid（12）。结论化合物5为新天然产物；化合物3～12为首次从总状蕨藻中分离得到，其中6和7首次发现于蕨藻属海藻中，3，4和8～12均为首次从海洋生物中分离得到；化合物2和5在10μmol／L浓度时对SH—SY5Y神经母细胞瘤细胞损伤显示了不同强度的保护作用；化合物1，2，5，11和12对蛋白酪氨酸磷酸酯酶1β均显示了明显的抑制活性。展开更多
文摘Objective:To evaluate the antibacterial and antioxidant activities and to identify the volatile bioactive compounds present in different crude extracts of the seaweed Caulerpa racemosa var.cylindracea.Methods:Caulerpa racemosa harvested from the intertidal zone of Mostaganem coast(N 35?54’37.94",E 0?3’17.37")was subjected to Soxhlet extraction using methanol,chloroform,and hexane solvents.Antioxidant properties were assessed by using 2,2’-diphenyl-1-picrylhydrazyl(DPPH),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)andβ-carotene bleaching assays.The antibacterial activity was evaluated on six standard bacterial strains using the agar disc diffusion method.The GC-MS analysis was performed using non-polar and polar capillary columns.Results:The chloroform extract of Caulerpa racemosa exhibited higher contents of polyphenols[(123.91±1.46)mg gallic acid equivalent/g dry extract]and tannins[(59.28±5.43)mg catechin equivalent/g dry extract](P<0.001)and was the most effective in scavenging DPPH[(1.98±0.08)mg/m L]and ABTS[(1.66±0.05)mg/m L]radicals.The hexane extract displayed the best antibacterial activity against Staphylococcus aureus,Bacillus cereus,and Pseudomonas aeruginosa,producing inhibition zones of(11.16±0.76),(9.00±0.00)and(9.33±1.15)mm,respectively.The l-(+)-ascorbic acid 2,6-dihexadecanoate and 4-hydroxy-2 methylproline were among the most abundant volatile compounds.Besides conventional fatty acids,cis-10-heptadecenoic acid,nonahexacontanoic acid,and dodecanoic acid,3-hydroxy-were identified.Two phytosterols were identified:stigmast-5-en-3-ol-(12.9%)and stigmast-5-en-3.beta.-ol,(24 S)-(4.57%).Conclusions:The preliminary identification of the volatile compounds reveals the presence of some new bioactive components not reported previously in Caulerpa racemosa from other geographical areas.Some of these compounds possess an interesting potential for pharmaceutical/nutraceutical applications.
基金the Team Project of Guangdong Natural Science Foundation(Grant No.039213)
文摘A polysaccharide, CrvpPS, was isolated from Caulerpa racemosa var peltata. It was reacted with nano-selenium in distilled water containing ascorbic acid (Vit C) to form a stable CrvpPS-nano-Se complex. The immunomodulatory effects of CrvpPS and CrvpPS-nano-Se on T lymphocytes subgroups and NK cells in mice were investigated. After intragastric administration for 10 days separately, both CrvpPS and CrvpPS-nano-Se showed significant stimulatory functions to thymus gland of mice. Moreover, the CrvpPS-nano-Se induced the percentage of CD3+, CD3+CD4+, NK cells and the CD4+/CD8+ value to increase significantly (P<0.05) when analyzed by flow cytometry, which is better than the CrvpPS, sucrose-nano-Se, and even the positive drug levamisole.
