[Objective]The research aimed to study the regeneration technology of Cazania rigens L.leaves and screen out the optimum medium formula for the regeneration of Cazania rigens L.leaves.[Method]Using Japan imported C.ri...[Objective]The research aimed to study the regeneration technology of Cazania rigens L.leaves and screen out the optimum medium formula for the regeneration of Cazania rigens L.leaves.[Method]Using Japan imported C.rigens leaves as materials,the orthogonal test was made for the callus and adventitious buds induction in MS medium with different kinds and concentrations of hormones.The optimum medium formula for the regeneration of C.rigens leaves were screened out.[Result]On the medium of MS ＋ 0.8-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,compact type and bright green calli were formed.When the leaves were inoculated on the medium of MS ＋ 0.5-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,many adventitious shoots can be induced and the induction rate reached 100%.When strong adventitious shoots with the height of 2.0-3.0 cm were transplanted into the medium of 1/2 MS ＋0.1 mg/L NAA,the rooting situations were good and the rooting rate was 100%.[Conclusion]The research provided a new way for the rapid propagation of C.rigens and laid the foundation for the genetic transformation and new varieties breeding of C.rigens.展开更多
G.rigens L.as a new quality ground cover plant can be applied in parks,yards,roadsides and tree pools,and contributes to the beauty of urban landscapes.This paper introduces biological characters and adaptability of‘...G.rigens L.as a new quality ground cover plant can be applied in parks,yards,roadsides and tree pools,and contributes to the beauty of urban landscapes.This paper introduces biological characters and adaptability of‘Mini Star White’,a new variety of G.rigens L.introduced from Japan,and studied the color improvement of the variety on the basis of applying it in various landscape forms,which enriches ground cover diversity of Suzhou area,and provides new materials for the hybridization of G.rigens L..展开更多
基金Supported by the Extra-curricular Academic Research Found of 12th Batch of Students in Soochow University (KY2010114A)Science and Technology Support (Agriculture) Project of Suzhou Province(SNG0908)~~
文摘[Objective]The research aimed to study the regeneration technology of Cazania rigens L.leaves and screen out the optimum medium formula for the regeneration of Cazania rigens L.leaves.[Method]Using Japan imported C.rigens leaves as materials,the orthogonal test was made for the callus and adventitious buds induction in MS medium with different kinds and concentrations of hormones.The optimum medium formula for the regeneration of C.rigens leaves were screened out.[Result]On the medium of MS ＋ 0.8-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,compact type and bright green calli were formed.When the leaves were inoculated on the medium of MS ＋ 0.5-1.0 mg/L TDZ ＋ 0.05-1.0 mg/L NAA,many adventitious shoots can be induced and the induction rate reached 100%.When strong adventitious shoots with the height of 2.0-3.0 cm were transplanted into the medium of 1/2 MS ＋0.1 mg/L NAA,the rooting situations were good and the rooting rate was 100%.[Conclusion]The research provided a new way for the rapid propagation of C.rigens and laid the foundation for the genetic transformation and new varieties breeding of C.rigens.
基金Sponsored by National Program for University Students’Innovation and Pioneering Training(201210285046)
文摘G.rigens L.as a new quality ground cover plant can be applied in parks,yards,roadsides and tree pools,and contributes to the beauty of urban landscapes.This paper introduces biological characters and adaptability of‘Mini Star White’,a new variety of G.rigens L.introduced from Japan,and studied the color improvement of the variety on the basis of applying it in various landscape forms,which enriches ground cover diversity of Suzhou area,and provides new materials for the hybridization of G.rigens L..
