Application of Soil Amendments Reduced Cadmium Bioavailability but Restrained Soybean Growth

The soybean is a crop which easily accumulates cadmium(Cd),which threatens human health.To assess the impact of the application of classic soil amendments on the Cd concentration in the soybean and the Cd bioavailability in the soil,a field study was conducted in Xiangtan Country(XT)and Liling City(LL),with inorganic-organic-microbial matter(T1)and silicon-calcium-magnesium oxide from natural minerals(T2)as two soil amendments in this study.The results indicated that the soil pH in the two sites increased significantly,up to 0.7~1.1 units and the Cd concentration in the stem,leaf,husk and seed in the two sites decreased differently.Of which,the soil available Cd in Xiangtan County(XT)decreased by 11.9%~16.0%,the enrichment factor(EF)and translocation factor(TF)reduced by 37.9%and 23.5%,respectively.Both soil amendments were effective in increasing the soil pH,reducing the seed and soil available Cd,but the soil organic matter,total N,stem length and grain yields decreased slightly.In conclusion,the Cd bioavailability was reduced but the soybean growth was restrained with the application of the soil amendments.

Mycorrhizal Inoculation Affects Pb and Cd Accumulation and Translocation in Pakchoi(Brassica chinensis L.)

Heavy metal(HM) contamination in soils is an environmental issue worldwide that threatens the quality and safety of crops and human health. A greenhouse experiment was carried out to investigate the growth, mycorrhizal colonization, and Pb and Cd accumulation of pakchoi(Brassica chinensis L. cv. Suzhou) in response to inoculation with three arbuscular mycorrhizal(AM) fungi(AMF), Funneliformis mosseae, Glomus versiforme, and Rhizophagus intraradices, aimed at exploring how AMF inoculation affected safe crop production by altering plant-soil interaction. The symbiotic relationship was well established between pakchoi and three AMF inocula even under Pb or Cd stress, where the colonization rates in the roots ranged from 24.5% to 38.5%. Compared with the non-inoculated plants, the shoot biomass of the inoculated plants increased by 8.7%–22.1% and 9.2%–24.3% in Pb and Cd addition treatments, respectively. Both glomalin-related soil protein(GRSP) and polyphosphate concentrations reduced as Pb or Cd concentration increased. Arbuscular mycorrhizal fungi inoculation significantly enhanced total absorbed Pb and Cd(except for a few samples) and increased the distribution ratio(root/shoot) in pakchoi at each Pb or Cd addition level. However, the three inocula significantly decreased Pb concentration in pakchoi shoots by 20.6%–67.5% in Pb addition treatments, and significantly reduced Cd concentration in the shoots of pakchoi in the Cd addition treatments(14.3%–54.1%), compared to the non-inoculated plants.Concentrations of Pb and Cd in the shoots of inoculated pakchois were all below the allowable limits of Chinese Food Safety Standard.The translocation factor of Pb or Cd increased significantly with increasing Pb or Cd addition levels, while there was no significant difference among the three AMF inocula at each metal addition level. Meanwhile, compared with the non-inoculated plants, AMF inocula significantly increased soil p H, electrical conductivity, and Pb or Cd concentrations in soil organic matter in the soils at the highest Pb or Cd dose after harvest of pakchoi, whereas the proportion of bioavailable Pb or Cd fraction declined in the AMF inoculated soil. Our study provided the first evidence that AM fungi colonized the roots of pakchoi and indicated the potential application of AMF in the safe production of vegetables in Pb or Cd contaminated soils.