Research Progress on Remediation Technology Suitable for Farmland Soil Contaminated by Cd

Cd contamination range of farmland soil in China is wide,which has constituted a major threat for food safety and residents＇ health. Via multi-year development,there are more remediation methods suitable for soil contaminated by Cd among the world,but some methods are only suitable for the contaminated sites and indoor research,and can not be applied in large-range contaminated farmland. We analyze the source of Cd in farmland soil. By combining farmland＇s characteristics,three remediation techniques（ low-absorption crops plantation,soil solidification and phytoremediation） and their effects are analyzed,and the existing problems and research prospects of the above techniques are explored. The research could provide reference for restoring farmland soil contaminated by Cd in China,recovering its production function and enhancing food safety standards.

Status of Heavy Metal Contamination in Paddy Soil of Guangxi Province and Effect of Silicon Fertilization on Cd Content in Brown Rice

[Objective] To provide a scientific basis for the management of Cd contamination, the status of Cd contamination in paddy soil of Guangxi Province, as well as the effect of silicon fertilization on Cd content in brown rice, was investigated. [Method] A total of 157 topsoil（0-20 cm） samples were collected from the major rice-growing paddy fields in Guangxi Province. The Cd contents in the topsoil samples were determined. The paddy soil environment quality and potential ecological risk were evaluated by single factor index and potential ecological index methods. In addition, in the Cd-contaminated paddy fields, the effects of different silicon fertilization treatments（no application（CK）, soil application of 750 kg/hm^2 silicon fertilizer（S）, leaf application of 1 500 L/hm^2 0.2% nanometer silicon（L）, soil application of 750 kg/hm^2 silicon fertilizer ＋ leaf application of 1 500 L/hm^2 0.2% nanometer silicon（SL）） on Cd content in brown rice were investigated. [Result] The total Cd contents in the 157 topsoil samples from paddy fields of Guangxi Province ranged from 0.02 to 7.33 mg/kg with an average content of 0.53 mg/kg. The Cd contents in 35.03% of the topsoil samples exceeded the grade II of national soil environment quality standards, and the topsoil samples were dominated by moderate and mild Cd contamination. Different silicon fertilization treatment all significantly reduced the Cd content in brown rice（P〈0.05）. In the treatment III, the Cd content in brown rice was lowest. Compared with that in the CK group, the Cd content in brown rice in the treatment III was reduced by 73.45%, in the treatment II was reduced by 62.07%, and in the treatment I was reduced by 34.48%. [Conclusion] The Cd in paddy fields of Guangxi Province showed a moderate to high ecological risk,and rational application of silicon fertilizer could effectively reduce the Cd content in brown rice.

Inhibition and Control Effects of Different Amendments on Cd Activation in Soil and Cd Absorption in Rice

Through pot tests of early rice and late rice in Cd polluted environment(soil Cd content,1.0 mg/kg),the inhibition and control effects of single application of organic fertilizers involving pig manure and biochar,and their combined application with alkaline amendments involving lime and oyster shell powder on soil Cd activation and rice Cd absorption were compared.And the correlation between soil available Cd and rice grain Cd was discussed.The results showed that compared with CK which did not involve amendments,the grain yields of early rice and late rice increased by 15.52%~36.01%and 12.02%~27.28%,respectively,the grain Cd content decreased by 24.76%~64.53%and 4.93%~58.32%,respectively,and the grain Cd accumulation decreased by 22.27%~51.76%and 33.84%~46.95%,respectively,except for single pig manure treatment.In particular,the Cd reduction effect of biochar was significantly better than that of pig manure,and the combined application with oyster shell powder was better than that with lime;after the harvest of early and late rice,the available Cd content in soil applied with amendments decreased by 35.00%~65.88%and 30.93%~68.57%,respectively,compared with CK.Further analysis showed that the soil available Cd content and rice grain Cd content could fit the linear equation well(R2>0.70,P<0.05),which demonstrated that the combined application of organic fertilizers and alkaline amendments can quickly reduce the Cd content of rice grain by passivating soil available Cd,and the combined application between biochar and oyster shell powder had a better effect.

Compound contamination and secondary ecological effects of Cd and As in soil－alfalfa ecosystems

d and As both have harmful effects on the growth,development and seed germination of alfalfa, especially in such a condition as the coexistence of Cd and As in soil environment The research using the pot-culture imitative method first found that if soil was simultaneously polluted by Cd and As,function of alfalfa absorbing Cd from soil may be promoted because of the existence of As,in conversely,Cd may inhibit alfalfa plant from absorbing As It was also found that secon- dary ecological effects were most likely to be brought out due to the coexistence of Cd and As. For example,alfalfa is passive to excessively absorb Cu and Pb .The harmful effects undoubtedly intensi- fy the contamination of alfalfa, The results showed that the mechanism of the interaction among Cd,As,Pb and Cu in soil-alfalfa ecosystems is very complicated.

The effects of different electrode materials on seed germination of Solanum nigrum L.and its Cd accumulation in soil

The effects of different electrode on Solanum nigrum L.seed germination were determined.The result showed that germination percentage(GP)of seeds in treatment T2(titanium elec-trode)was 26.6%higher than in control(CK,without electric field).High potassium and calcium concentrations were beneficial for seed enzymatic activity in treatment T2,which could partly explain the increase in GP.Cd accumulation(μg/pot)in S.nigrum treated with any electric field was significantly higher(p<0.05)than in CK without electric field.Specif-ically,Cd accumulation under the treatment T3(stainless steel electrode)was the highest both in roots and shoots;this accumulation in shoots and roots were 74.7%and 67.4%higher for stainless steel than in CK.This increase must have been associated with a higher Cd concentration in plants and did not exert a significant effect on the biomass.In par-ticular,Cd concentrations in roots and shoots under stainless steel treatment were both significantly higher than in CK(p<0.05),which had to be related to the higher available Cd concentration in the soil in the middle region.Furthermore,it could be attributed to al-tered soil pH and other soil properties.Moreover,none of the biomasses were significantly affected(p<0.05)by different electrode materials compared to CK.

A Meta-Analysis on Phenotypic Variation in Cadmium Accumulation of Rice and Wheat:Implications for Food Cadmium Risk Control

In some densely-populated countries, farmland has been widely cadmium (Cd) contaminated, and the utilization of the contaminated farmland for crop production is currently unavoidable. This necessitates the use of low-Cd crops (i.e., pollution-safe cultivars, the crop varieties with the ability to accumulate a low level of Cd in their edible parts when grown on polluted soil) in these areas and highlights the importance of knowledge on phenotypic variation in crop Cd accumulation for food Cd risk control. Studies on phenotypic variation in heavy metal accumulation started decades ago for a wide range of crops, and synthesis of the scattered experimental results in the literature is in need. We built a Low-Cd Crops Database based on literature research, and relevant meta-analysis was performed to quantitatively explore the phenotypic variation in Cd uptake and translocation of rice and wheat. Considerable variability existed among rice (median grain Cd bioconce nt ration factor (BCF) of 0.10) and wheat (median grain Cd BCF of 0.21) phenotypes in grain Cd accumulation, and this variability was labile to soil pH and the level of Cd stress. Wheat statistically had a higher root-to-shoot Cd-translocating ability than rice, highlighting potential food Cd risks and the importance of growing low-Cd wheat in slightly Cd-contaminated regions. Meanwhile, no correlations were detected among soil-to-root, root-to-shoot, and shoot-to-grain translocation factors, implying that Cd uptake and internal translocation in crops were probably controlled by different underlying gene tic mechanisms. Root-to-shoot Cd transport could be a favorable target trait for selecting and breeding low-Cd rice and wheat. In all, this review provides a comprehensive low-Cd crop list for remediation practice and a systematic meta-analysis inferring food Cd risks based on plant capacity for Cd accumulation and desired traits for low-Cd crop breeding.

Rice(Oryza sativa L.)seedlings enriched with zinc or manganese:Their impacts on cadmium accumulation and expression of related genes

Cadmium(Cd)contamination in paddy soils means that the rice produced there may be unsafe for human consumption.A hydroponic study was conducted to enrich rice seedlings with zinc(Zn)or manganese(Mn),and the uptake and transport characteristics of Cd in these Zn-and Mn-rich seedlings were subsequently investigated using a greenhouse pot trial.The results showed that hydroponic cultivation in 10-50μmol L^(-1) Zn(ZnSO_(4)·7 H_(2)O)or50-250μmol L^(-1) Mn(MnSO_(4)·H_(2)O)for 30 d had no significant impact on rice growth,while the accumulation of Zn and Mn was 7.31-18.5 and 25.4-47.7 times higher,respectively,than in the control(no Zn or Mn addition).The accumulation of Cd in the Zn-and Mn-rich rice plants was 26.3%-38.6% and 34.4%-44.5% lower than that in the control,respectively,and the translocation factors of Cd from roots to shoots also decreased by 23.3%-41.3% and 25.3%-37.0%,respectively,after transplanting to Cd-contaminated soils.Furthermore,the relative expression levels of OsIRT1(Oryza sativa iron-regulated transporter 1)were downregulated by 40.1%-59.3% and 16.0%-25.9%,respectively,in the Zn-and Mn-rich seedling roots.This downregulation may indicate a possible mechanism contributing to the reductions in Cd absorption.Field experiments confirmed that the Zn-and Mn-rich seedlings produced brown rice(unpolished rice grains)with significantly decreased concentrations of Cd(34.2%-44.4%).This study provides an innovative method for reducing the food safety risks from rice grown on slightly to moderately Cd-contaminated paddy soils.