Rat Liver Cd5Zn2-metallothionein isoforms were titrated with cuprous ion and monitored by 1H NMR Initial replacement occurred in β-domain followed by α-domain.MT exhibited well-defined structure under 10 mole equiva...Rat Liver Cd5Zn2-metallothionein isoforms were titrated with cuprous ion and monitored by 1H NMR Initial replacement occurred in β-domain followed by α-domain.MT exhibited well-defined structure under 10 mole equivalents cuprous ion.展开更多
The kinetics of zinc transfer reaction between rat liver Cd_5Zn_2-MT and apo-carbonic anhydrase has been studied.Low molecular weight ligands (LMWL) present different effect on the zinc transfer reaction.A possible as...The kinetics of zinc transfer reaction between rat liver Cd_5Zn_2-MT and apo-carbonic anhydrase has been studied.Low molecular weight ligands (LMWL) present different effect on the zinc transfer reaction.A possible association mechanism has been proposed,where the formation of protein-protein complex may be the rate-determining step.展开更多
文摘Rat Liver Cd5Zn2-metallothionein isoforms were titrated with cuprous ion and monitored by 1H NMR Initial replacement occurred in β-domain followed by α-domain.MT exhibited well-defined structure under 10 mole equivalents cuprous ion.
基金Project supported by the National Natural Science Foundation of China.
文摘The kinetics of zinc transfer reaction between rat liver Cd_5Zn_2-MT and apo-carbonic anhydrase has been studied.Low molecular weight ligands (LMWL) present different effect on the zinc transfer reaction.A possible association mechanism has been proposed,where the formation of protein-protein complex may be the rate-determining step.
