Cdc25a在跨种属肝癌组织中的表达及其意义

目的:研究细胞周期通路中的细胞分裂周期蛋白25a(Cdc25a)在人、大鼠和树鼩不同种属的肝癌、癌旁及正常肝组织中的表达和意义,进一步验证跨种属筛选肝癌关键基因研究策略的可行性。方法:采用实时荧光定量PCR和Western blotting技术检测人、大鼠和树鼩的肝癌及其相应癌旁组织以及正常肝组织中Cdc25a mRNA和蛋白表达水平。结果:Cdc25a mRNA表达水平在人、大鼠和树鼩的肝癌组织中均高于正常肝组织(均P<0.05);在人和大鼠肝癌组织中的表达高于其对应的癌旁组织(P<0.05);其余各组织间mRNA表达水平的差别无统计学意义(均P>0.05)。Cdc25a mRNA在人肝癌组织中的表达水平与病人的临床分期、门脉癌栓及肝外转移明显相关,而与术后复发、肿瘤直径、肿瘤个数、血清甲胎蛋白水平和肿瘤分化无明显关系。Western blotting检测结果显示Cdc25a在人、大鼠和树鼩肝癌组织中的蛋白表达水平均较癌旁和正常肝组织显著上调。结论:Cdc25a有可能是影响肝癌发生和发展的重要分子之一,跨种属筛选肿瘤关键基因策略可能有助于发现肝癌关键基因。

Protein stability regulators screeningassay (Pro-SRSA)： protein degradation meets theCRISPR-Cas9 library

The regulation of protein stability is a fundamental issue for biophysical processes,but there has not previously been a convenient and unbiased method of identifying regulators of protein stability.However,as reported in the article entitled "A genome-scale CRISPR-Cas9 screening method for protein stability reveals novel regulators of Cdc25 A," recently published in Cell Discovery,our team developed a protein stability regulators screening assay(Pro-SRSA) by combining the whole-genome clustered regularly interspaced short palindromic repeats Cas9(CRISPR-Cas9) library with a dual-fluorescence-based protein stability reporter and high-throughput sequencing to screen for regulators of protein stability.Based on our findings,we are confident that this efficient and unbiased screening method at the genome scale will be used by researchers worldwide to identify regulators of protein stability.

乳腺癌中CDC25A和P57kip2蛋白表达与病理分级及临床分期的关系

目的探究乳腺癌检测中患者病理分级、临床分期与CDC25A、P57kip2蛋白的相关性。方法选取2016年4月至2019年5月本院收治的120例乳腺癌患者,采用免疫组化法对癌细胞中CDC25A、P57kip2蛋白的表达进行检测,按患者病理分级分为:Ⅰ级44例,Ⅱ级36例,Ⅲ级40例;按照患者临床分期分为:Ⅰ期35例,Ⅱ期40例,Ⅲ期45例,比较两种蛋白表达与临床分期、病理分级的相关性。结果年龄与P57kip2、CDC25A表达无相关性。临床分期、病理分级、淋巴结转移与P57kip2、CDC25A表达有相关性,随着分级、分期及转移程度加深,P57kip2、CDC25A表达率下降(P<0.05)。结论乳腺癌诊断过程中可将P57kip2、CDC25A表达情况作为乳腺癌诊断预测因子,进而判断患者癌症程度,值得临床推广应用。