Objective To investigate the prevalence of cedar pollinosis in China, and compare Cryptomeria fortunei(Cf) with Cryptomeria japonica (Cj) morphologically. Methods\ An epidemiological survey on nasal allergy was carri...Objective To investigate the prevalence of cedar pollinosis in China, and compare Cryptomeria fortunei(Cf) with Cryptomeria japonica (Cj) morphologically. Methods\ An epidemiological survey on nasal allergy was carried out among 1 660 primary and middle school students and 1 620 university students, including questionnaire investigation, nasal inspection by the same doctor and scratch test with the allergen extract of Cj. The pollen of Cf and Cj were observed by optical and electron microscope. The genes of chloroplast of them were analysed. Results ①The total positive rate of cedar pollen scratch test was 4.2%. 55 cases of nasal allergy were confirmed according to the criteria of diagnosis, in which 8 cases showed positive reaction to Cj and only 1 case was hypersentive to it. ②No significant distinction between Cf and Cj was found by optical and electron microscope and no difference in gene analysis was found. Conclusion\ ①Cedar pollinosis does exist in China. It′s low prevbalence is related to the low quantity of cedar pollen.②Cf and Cj are of the same family and genus. ③There may be an increasing tendency of cedar pollinosis in China.\;展开更多
This study examined spatial variations in the concentration,grain size and heavy mineral assemblages on Cedar Beach(Lake Erie,Canada).Magnetic studies of heavy mineral-enriched,dark-reddish sands present on the beac...This study examined spatial variations in the concentration,grain size and heavy mineral assemblages on Cedar Beach(Lake Erie,Canada).Magnetic studies of heavy mineral-enriched,dark-reddish sands present on the beach showed that magnetite(~150μm) is the dominant magnetic mineral.Surficial magnetic susceptibility values defined three zones:a lakeward region close to the water line(Zone 1),the upper swash zone(Zone 2) and the region landwards of the upper swash zone (Zone 3).Zone 2 showed the highest bulk and mass susceptibility(κ,χ) and the highest mass percentage of smaller grain-size(250μm) fractions in the bulk sand sample.Susceptibility(i.e.κandχ) values decreased and grain size coarsened from Zone 2 lakewards(into Zone 1) and landwards (into Zone 3),and correlated with the distribution of the heavy mineral assemblage,most probably reflecting preferential separation of large,less dense particles by waves and currents both along and across the beach.The eroded western section of Cedar Beach showed much higher concentrations of heavy minerals including magnetite,and finer sand grain sizes than the accreting eastern section, suggesting that magnetic techniques could be used as a rapid,cost-effective way of examining erosion along sensitive coastline areas.展开更多
Slash and burn practices are widely used around the globe with different degrees of success which are mostly related to the impact of fire on soil properties. In Japan slash and burn practises, known as Yakihata, have...Slash and burn practices are widely used around the globe with different degrees of success which are mostly related to the impact of fire on soil properties. In Japan slash and burn practises, known as Yakihata, have a long history and are still used in Yamagata Prefecture today. The purpose of this study was to determine the impact of a low severity controlled fire underneath Japanese cedar (Cryptomeria japonica) on brown forest soil (Cambisol). Japanese Cedar is the dominant species among plantations in Japan. We measured organic carbon and nitrogen content as well as changes in carbon (δ13C) and nitrogen (δ15N) stable isotope composition in a steep west facing slope under heavy precipitation (~2600 mm/a) and heavy snowfall (~3 to 4 m/a). The accumulation of Ctotal and Ntotal at the bottom of the slopes was remarkably higher at the slash and burned site than in the control forest site. After slash and burn δ15N isotopes in the slope in general became significantly lighter than in the control forest while the δ13C did not show any significant difference between the two sites except at the bottom of the slopes where δ13C was heavier in the forest. The results show that Ctotal and Ntotal values as well as the isotopes ratios of C and N change with decreasing elevation in the forest as well as in the burned site being consistent with leaching and erosion. The changes in soil nitrogen and carbon isotopes at the bottom of the slope appear to be related to the transport of material with different isotopic composition from the upper slope. The effect of the low severity fire (as part of the slash and burn practice) on soil organic carbon and nitrogen movement was enhanced by the steepness of the slopes and the high precipitation of Shonai region.展开更多
Environmental magnetic research on beaches and shoreline processes is limited.Therefore,we carried out environmental magnetic studies on the heavy mineral-enriched,dark-reddish sands on Cedar Beach of western Lake Eri...Environmental magnetic research on beaches and shoreline processes is limited.Therefore,we carried out environmental magnetic studies on the heavy mineral-enriched,dark-reddish sands on Cedar Beach of western Lake Erie(41.68°N,82.40°W).Magnetite has been identified as the dominant magnetic mineral of these sands.This study reveals a spatial variation in concentration of magnetite particles,distribution of展开更多
The relation between runoff and sediment and land cover is investigated in the Cedar Creek Watershed (CCW), located in Northeastern Indiana, United States. The major land cover types in this watershed are cultivated...The relation between runoff and sediment and land cover is investigated in the Cedar Creek Watershed (CCW), located in Northeastern Indiana, United States. The major land cover types in this watershed are cultivated land, woodland and pasture /Conservation Reserve Program (CRP), which account for approximate 90 % of the total area in the region. Moreover, land use was changed tremendously from aooo to 9004, even without regarding the effect of the crop rotation system (corn & soybean). At least 49 % of land cover types were changed into other types in this period. The land cover types, ranking by changing area from high to low series, are rye, soybean, corn, woodland and pasture/CRP. The CCW is divided into 21 subwatersheds, and soil and water loss in each sub-watershed is computed by using Soil and Water Assessment Tool (SWAT). The results indicate that the variations in runoff and sediment have positive relation to the area of crops (especially corn and soybean); sediment is more sensitive to land cover changes than runoff; more heavy rainfall does not always mean more runoff because the combination of different land cover types always modify runoff coefficient; and rye, soybean and corn are the key land cover types, which affected the variation in runoff and sediment in the CCW.展开更多
This work proposes the use of experimental design for optimization of microwave-assisted digestion of bio- morphic ZrO2-ceramic. Cedar wood is used as template and it was infiltrated with ZrO2 via sol-gel process. XPS...This work proposes the use of experimental design for optimization of microwave-assisted digestion of bio- morphic ZrO2-ceramic. Cedar wood is used as template and it was infiltrated with ZrO2 via sol-gel process. XPS, SEM and XRD have been used to verify the effectiveness of the synthesis. The effects of different combination of acids in the digestion of this sample have been optimized taking as response the concentra- tion of several metallic ions measured by ICP-MS.展开更多
The topic of carbon sequestration in forest ecosystems has recently taken a lot of attention due to concerns about global climate change. As a spontaneous species of the Central Middle Atlas, the Atlas Cedar was the s...The topic of carbon sequestration in forest ecosystems has recently taken a lot of attention due to concerns about global climate change. As a spontaneous species of the Central Middle Atlas, the Atlas Cedar was the subject of our study. Thus, a total of 30 sample trees were subjected to the weight measurements for the estimation of biomass and organic carbon stock in a logging lot in the Azrou forest. The amount of sequestered carbon was then extrapolated in all stand forests studied from the developed carbomass models. The results obtained show that the largest allocation of carbon stock (93%) is in the stem, followed by branches (5%) and foliage (2%). The average carbon storage in this cedar forest is 99.42 tC/ha (aboveground and belowground), or 364.58 t/ha of CO2, a value significantly higher than that found in other Moroccan forest ecosystems.展开更多
The Atlas cedar (Cedrus atlantica) is one of the more valuable reforestation species in the Mediterranean areas. But this species suffers from rainfall limitation and climate changes, particularly in its originated ar...The Atlas cedar (Cedrus atlantica) is one of the more valuable reforestation species in the Mediterranean areas. But this species suffers from rainfall limitation and climate changes, particularly in its originated area, North Africa. Therefore, any knowledge about the plantation practices to improve the tree water availability and the reforestation success has great importance. The current study has been undertaken in this view, i.e. to compare the growth of cedar plots located in El M’Sid Mountains, Souk Ahras department (700 km east of Algiers), according to different planting methods. The radial growth and the productivity have been measured on a cedar plot with a total surface of 165 ha. 150 ha has been planted in 1970 on hillside ditches, and 15 ha of which the majority is planted in a simple hole and some bouquets on terraces, both are originated from a complementary reforestation achieved during 1980. The results show that most of the trees planted in 1970 reach a height ranging between 10 and 17 m and a diameter between 23 and 44 cm. Even some trees were 20 m high with diameter of 65 cm. The productivity fluctuates between 3 and 8 m3/ha/year, values close to the one of natural native plots. Trees planted in 1980 reach a height ranging between 5. 5 to 7 m, and diameter between 11.5 to 23 cm. The radial growth improves that the hillside ditches help the roots anchoring and trees growth during the first season after plantation. These results indicate that the cedar tree can be used with success in areas outside the natural cedar forest presence, and that appropriate planting techniques can compensate in part the lower rainfall occurring in these chosen regeneration areas.展开更多
The ultrasonic wave velocities of Japanese cedar columns were measured using a non-contact method. An air-coupled ultrasonic wave was propagated through the axial and lateral directions of wood. The velocities in the ...The ultrasonic wave velocities of Japanese cedar columns were measured using a non-contact method. An air-coupled ultrasonic wave was propagated through the axial and lateral directions of wood. The velocities in the axial direction (V<sub>L</sub>) showed the minimum values around the pith. The averaged V<sub>L</sub> increased from 3600 m/s towards the outside of measurement area and attained the maximum values (=4010 m/s). The velocities in the lateral direction (V<sub>RT </sub>) showed no tendency among measurement points. The averaged V<sub>RT </sub> was 1450 m/s. The velocities obtained using the non-contact method showed a significant positive relationship with those obtained using the contact method. The averaged ratio of V<sub>L</sub> to V<sub>RT </sub> was measured to be approximately 2.2 to 2.8. These ratios were in agreement with those from a contact method. These findings suggest that it is possible to measure the velocity in Japanese cedar columns with the non-contact method by using air-coupled ultrasonics.展开更多
The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase...The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase chain reaction(qRT-PCR)assay is pivotal for the early warning of the potential of zoonotic infectious diseases.Specific primers and probes were designed for the relatively conserved regions based on whole genome sequences of Langya virus(LayV),Mojiang virus(MojV),Nipah virus(NiV),and Cedar virus(CedV),followed by the establishment of a quadruple real-time fluorescence-based qRT-PCR detection method.No cross-reactivity was observed with other viral nucleic acids.The optimal linear detection range for LayV,MojV,NiV,and CedV was 10^(1)-10^(8)copies/μL,and the lower limit of detection was 10 copies/μL.Three different DNA concentrations of LayV,MojV,NiV,and CedV(10^(4),10^(5),and 10^(6)copies/μL)were tested 14 times,achieving good repeatability.The standard deviation of the cycle threshold values for each concentration was<0.5 and the coefficient of variation was<3%.Furthermore,the amplification efficiency of quadruple real-time fluorescence-based qRT-PCR was>90%,and the correlation coefficient was>0.99.The established quadru-ple real-time fluorescence-based qRT-PCR assay for the detection of LayV,MojV,NiV,and CedV exhibits good sensitivity,specificity,and repeatability.Therefore,it can be used to detect Henipavirus and other related clinical specimens.展开更多
Moso bamboo expansions into Japanese cedar forests are common.The expansion effects on soil nitrous oxide(N_(2)O)emissions have not been thoroughly understood,and the underlying microbial mechanisms remain unclear.We ...Moso bamboo expansions into Japanese cedar forests are common.The expansion effects on soil nitrous oxide(N_(2)O)emissions have not been thoroughly understood,and the underlying microbial mechanisms remain unclear.We studied bacterial and fungal contribution to soil N_(2)O emissions under moso bamboo or Japanese cedar by applying bacterial or fungal inhibitors using streptomycin and iprodione,respectively.Soil N_(2)O emissions were measured and the relative contribution of bacteria and fungi to soil N_(2)O emissions was calculated.N_(2)O emission from soil with moso bamboo was significantly higher than under Japanese cedar.Compared with control,bacterial or fungal inhibitor or their combination decreased N_(2)O emissions,indicating substantial contribution of microbial activities to N_(2)O emissions.However,the relative contribution of bacteria and fungi to N_(2)O emissions was not affected by plants.Soil organic carbon,total and ammonium nitrogen were lower in soil under moso bamboo than Japanese cedar,suggesting faster microbial decomposition under moso bamboo.Fungal inhibitor and plants interactively affected soil pH,total phosphorus and ammonium nitrogen,while bacterial inhibitor and plants interactively affected total nitrogen,indicating substantial dependence of effects by microbial communities on plant species.Moso bamboo and Japanese cedar differed in their effects on soil N_(2)O emissions with higher emissions under moso bamboo.Stimulation of N_(2)O emission under moso bamboo might occur due to higher nitrogen mineralization and subsequent denitrification induced by high root exudation.These results highlight the need to consider the effect of species shifts on N_(2)O emissions in forests.展开更多
基金This paper is granted by the fund of Japanese-ChineseMedical A ssociation!( N o110 )
文摘Objective To investigate the prevalence of cedar pollinosis in China, and compare Cryptomeria fortunei(Cf) with Cryptomeria japonica (Cj) morphologically. Methods\ An epidemiological survey on nasal allergy was carried out among 1 660 primary and middle school students and 1 620 university students, including questionnaire investigation, nasal inspection by the same doctor and scratch test with the allergen extract of Cj. The pollen of Cf and Cj were observed by optical and electron microscope. The genes of chloroplast of them were analysed. Results ①The total positive rate of cedar pollen scratch test was 4.2%. 55 cases of nasal allergy were confirmed according to the criteria of diagnosis, in which 8 cases showed positive reaction to Cj and only 1 case was hypersentive to it. ②No significant distinction between Cf and Cj was found by optical and electron microscope and no difference in gene analysis was found. Conclusion\ ①Cedar pollinosis does exist in China. It′s low prevbalence is related to the low quantity of cedar pollen.②Cf and Cj are of the same family and genus. ③There may be an increasing tendency of cedar pollinosis in China.\;
基金supported by funding from the 111 Project B07011 of Ministry of Education of China,the China Scholarship Council(CSC) to SWZ (NCIS No.2007103928)an NSERC grant to MTC. D.Chevalier is thanked for her help in sampling. Laboratory assistance was provided bv K.Kawasaki and S.Joshi
文摘This study examined spatial variations in the concentration,grain size and heavy mineral assemblages on Cedar Beach(Lake Erie,Canada).Magnetic studies of heavy mineral-enriched,dark-reddish sands present on the beach showed that magnetite(~150μm) is the dominant magnetic mineral.Surficial magnetic susceptibility values defined three zones:a lakeward region close to the water line(Zone 1),the upper swash zone(Zone 2) and the region landwards of the upper swash zone (Zone 3).Zone 2 showed the highest bulk and mass susceptibility(κ,χ) and the highest mass percentage of smaller grain-size(250μm) fractions in the bulk sand sample.Susceptibility(i.e.κandχ) values decreased and grain size coarsened from Zone 2 lakewards(into Zone 1) and landwards (into Zone 3),and correlated with the distribution of the heavy mineral assemblage,most probably reflecting preferential separation of large,less dense particles by waves and currents both along and across the beach.The eroded western section of Cedar Beach showed much higher concentrations of heavy minerals including magnetite,and finer sand grain sizes than the accreting eastern section, suggesting that magnetic techniques could be used as a rapid,cost-effective way of examining erosion along sensitive coastline areas.
文摘Slash and burn practices are widely used around the globe with different degrees of success which are mostly related to the impact of fire on soil properties. In Japan slash and burn practises, known as Yakihata, have a long history and are still used in Yamagata Prefecture today. The purpose of this study was to determine the impact of a low severity controlled fire underneath Japanese cedar (Cryptomeria japonica) on brown forest soil (Cambisol). Japanese Cedar is the dominant species among plantations in Japan. We measured organic carbon and nitrogen content as well as changes in carbon (δ13C) and nitrogen (δ15N) stable isotope composition in a steep west facing slope under heavy precipitation (~2600 mm/a) and heavy snowfall (~3 to 4 m/a). The accumulation of Ctotal and Ntotal at the bottom of the slopes was remarkably higher at the slash and burned site than in the control forest site. After slash and burn δ15N isotopes in the slope in general became significantly lighter than in the control forest while the δ13C did not show any significant difference between the two sites except at the bottom of the slopes where δ13C was heavier in the forest. The results show that Ctotal and Ntotal values as well as the isotopes ratios of C and N change with decreasing elevation in the forest as well as in the burned site being consistent with leaching and erosion. The changes in soil nitrogen and carbon isotopes at the bottom of the slope appear to be related to the transport of material with different isotopic composition from the upper slope. The effect of the low severity fire (as part of the slash and burn practice) on soil organic carbon and nitrogen movement was enhanced by the steepness of the slopes and the high precipitation of Shonai region.
文摘Environmental magnetic research on beaches and shoreline processes is limited.Therefore,we carried out environmental magnetic studies on the heavy mineral-enriched,dark-reddish sands on Cedar Beach of western Lake Erie(41.68°N,82.40°W).Magnetite has been identified as the dominant magnetic mineral of these sands.This study reveals a spatial variation in concentration of magnetite particles,distribution of
文摘The relation between runoff and sediment and land cover is investigated in the Cedar Creek Watershed (CCW), located in Northeastern Indiana, United States. The major land cover types in this watershed are cultivated land, woodland and pasture /Conservation Reserve Program (CRP), which account for approximate 90 % of the total area in the region. Moreover, land use was changed tremendously from aooo to 9004, even without regarding the effect of the crop rotation system (corn & soybean). At least 49 % of land cover types were changed into other types in this period. The land cover types, ranking by changing area from high to low series, are rye, soybean, corn, woodland and pasture/CRP. The CCW is divided into 21 subwatersheds, and soil and water loss in each sub-watershed is computed by using Soil and Water Assessment Tool (SWAT). The results indicate that the variations in runoff and sediment have positive relation to the area of crops (especially corn and soybean); sediment is more sensitive to land cover changes than runoff; more heavy rainfall does not always mean more runoff because the combination of different land cover types always modify runoff coefficient; and rye, soybean and corn are the key land cover types, which affected the variation in runoff and sediment in the CCW.
文摘This work proposes the use of experimental design for optimization of microwave-assisted digestion of bio- morphic ZrO2-ceramic. Cedar wood is used as template and it was infiltrated with ZrO2 via sol-gel process. XPS, SEM and XRD have been used to verify the effectiveness of the synthesis. The effects of different combination of acids in the digestion of this sample have been optimized taking as response the concentra- tion of several metallic ions measured by ICP-MS.
文摘The topic of carbon sequestration in forest ecosystems has recently taken a lot of attention due to concerns about global climate change. As a spontaneous species of the Central Middle Atlas, the Atlas Cedar was the subject of our study. Thus, a total of 30 sample trees were subjected to the weight measurements for the estimation of biomass and organic carbon stock in a logging lot in the Azrou forest. The amount of sequestered carbon was then extrapolated in all stand forests studied from the developed carbomass models. The results obtained show that the largest allocation of carbon stock (93%) is in the stem, followed by branches (5%) and foliage (2%). The average carbon storage in this cedar forest is 99.42 tC/ha (aboveground and belowground), or 364.58 t/ha of CO2, a value significantly higher than that found in other Moroccan forest ecosystems.
文摘The Atlas cedar (Cedrus atlantica) is one of the more valuable reforestation species in the Mediterranean areas. But this species suffers from rainfall limitation and climate changes, particularly in its originated area, North Africa. Therefore, any knowledge about the plantation practices to improve the tree water availability and the reforestation success has great importance. The current study has been undertaken in this view, i.e. to compare the growth of cedar plots located in El M’Sid Mountains, Souk Ahras department (700 km east of Algiers), according to different planting methods. The radial growth and the productivity have been measured on a cedar plot with a total surface of 165 ha. 150 ha has been planted in 1970 on hillside ditches, and 15 ha of which the majority is planted in a simple hole and some bouquets on terraces, both are originated from a complementary reforestation achieved during 1980. The results show that most of the trees planted in 1970 reach a height ranging between 10 and 17 m and a diameter between 23 and 44 cm. Even some trees were 20 m high with diameter of 65 cm. The productivity fluctuates between 3 and 8 m3/ha/year, values close to the one of natural native plots. Trees planted in 1980 reach a height ranging between 5. 5 to 7 m, and diameter between 11.5 to 23 cm. The radial growth improves that the hillside ditches help the roots anchoring and trees growth during the first season after plantation. These results indicate that the cedar tree can be used with success in areas outside the natural cedar forest presence, and that appropriate planting techniques can compensate in part the lower rainfall occurring in these chosen regeneration areas.
文摘The ultrasonic wave velocities of Japanese cedar columns were measured using a non-contact method. An air-coupled ultrasonic wave was propagated through the axial and lateral directions of wood. The velocities in the axial direction (V<sub>L</sub>) showed the minimum values around the pith. The averaged V<sub>L</sub> increased from 3600 m/s towards the outside of measurement area and attained the maximum values (=4010 m/s). The velocities in the lateral direction (V<sub>RT </sub>) showed no tendency among measurement points. The averaged V<sub>RT </sub> was 1450 m/s. The velocities obtained using the non-contact method showed a significant positive relationship with those obtained using the contact method. The averaged ratio of V<sub>L</sub> to V<sub>RT </sub> was measured to be approximately 2.2 to 2.8. These ratios were in agreement with those from a contact method. These findings suggest that it is possible to measure the velocity in Japanese cedar columns with the non-contact method by using air-coupled ultrasonics.
基金supported by the National Key R&D Program of China(Grant No.2022YFC2601200).
文摘The emerging viruses within the genus Henipavirus in the family Paramyxoviridae pose a great threat to public biosafety.To develop a quadruple real-time fluorescence-based quantitative reverse transcription polymerase chain reaction(qRT-PCR)assay is pivotal for the early warning of the potential of zoonotic infectious diseases.Specific primers and probes were designed for the relatively conserved regions based on whole genome sequences of Langya virus(LayV),Mojiang virus(MojV),Nipah virus(NiV),and Cedar virus(CedV),followed by the establishment of a quadruple real-time fluorescence-based qRT-PCR detection method.No cross-reactivity was observed with other viral nucleic acids.The optimal linear detection range for LayV,MojV,NiV,and CedV was 10^(1)-10^(8)copies/μL,and the lower limit of detection was 10 copies/μL.Three different DNA concentrations of LayV,MojV,NiV,and CedV(10^(4),10^(5),and 10^(6)copies/μL)were tested 14 times,achieving good repeatability.The standard deviation of the cycle threshold values for each concentration was<0.5 and the coefficient of variation was<3%.Furthermore,the amplification efficiency of quadruple real-time fluorescence-based qRT-PCR was>90%,and the correlation coefficient was>0.99.The established quadru-ple real-time fluorescence-based qRT-PCR assay for the detection of LayV,MojV,NiV,and CedV exhibits good sensitivity,specificity,and repeatability.Therefore,it can be used to detect Henipavirus and other related clinical specimens.
基金supported by the National Natural Science Foundation of China(31770749)Research Funding of Lushan National Forest Ecosystem Research Station(9022206523).
文摘Moso bamboo expansions into Japanese cedar forests are common.The expansion effects on soil nitrous oxide(N_(2)O)emissions have not been thoroughly understood,and the underlying microbial mechanisms remain unclear.We studied bacterial and fungal contribution to soil N_(2)O emissions under moso bamboo or Japanese cedar by applying bacterial or fungal inhibitors using streptomycin and iprodione,respectively.Soil N_(2)O emissions were measured and the relative contribution of bacteria and fungi to soil N_(2)O emissions was calculated.N_(2)O emission from soil with moso bamboo was significantly higher than under Japanese cedar.Compared with control,bacterial or fungal inhibitor or their combination decreased N_(2)O emissions,indicating substantial contribution of microbial activities to N_(2)O emissions.However,the relative contribution of bacteria and fungi to N_(2)O emissions was not affected by plants.Soil organic carbon,total and ammonium nitrogen were lower in soil under moso bamboo than Japanese cedar,suggesting faster microbial decomposition under moso bamboo.Fungal inhibitor and plants interactively affected soil pH,total phosphorus and ammonium nitrogen,while bacterial inhibitor and plants interactively affected total nitrogen,indicating substantial dependence of effects by microbial communities on plant species.Moso bamboo and Japanese cedar differed in their effects on soil N_(2)O emissions with higher emissions under moso bamboo.Stimulation of N_(2)O emission under moso bamboo might occur due to higher nitrogen mineralization and subsequent denitrification induced by high root exudation.These results highlight the need to consider the effect of species shifts on N_(2)O emissions in forests.