Despite recent advances in surgical techniques and perioperative management, the prognosis of pancreatic cancer(PCa) remains extremely poor. To provide optimal treatment for each patient with Pca, superior biomarkers ...Despite recent advances in surgical techniques and perioperative management, the prognosis of pancreatic cancer(PCa) remains extremely poor. To provide optimal treatment for each patient with Pca, superior biomarkers are urgently needed in all phases of management from early detection to staging, treatment monitoring, and prognosis. In the blood of patients with cancer, circulating tumor cells(CTCs) and cell-free nucleic acids(cf NAs), such as DNA, m RNA, and noncoding RNA have been recognized. In the recent years, their presence in the blood has encouraged researchers to investigate their potential use as novel blood biomarkers, and numerous studies have demonstrated their potential clinical utility as a biomarker for certain types of cancer. This concept, called "liquid biopsy" has been focused on as a less invasive, alternative approach to cancer tissue biopsy for obtaining genetic and epigenetic aberrations that contribute to oncogenesis and cancer progression. In this article, we review the available literature on CTCs and cfN As in patients with cancer, particularly focusing on PCa, and discuss future perspectives in this field.展开更多
目的 :探索恶性胸腹水上清中游离胸苷酸合成酶(TS)、β-tubulinⅢm RNA的表达,以及游离TS、β-tubulinⅢ表达和患者5-氟尿嘧啶(5-FU)、多西紫杉醇药物敏感性的关系。方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离、提取游离核酸,分离...目的 :探索恶性胸腹水上清中游离胸苷酸合成酶(TS)、β-tubulinⅢm RNA的表达,以及游离TS、β-tubulinⅢ表达和患者5-氟尿嘧啶(5-FU)、多西紫杉醇药物敏感性的关系。方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离、提取游离核酸,分离肿瘤细胞。实时荧光定量RT-PCR分别检测游离上清和肿瘤细胞中TS和β-tubulinⅢm RNA表达;ATP-TCA方法检测肿瘤细胞5-FU、多西紫杉醇的敏感性。结果:TS和β-tubulinⅢm RNA在恶性胸/腹水游离上清中的检出率为98%。与配对的肿瘤细胞相比,游离上清中TS m RNA表达水平高(P=0.02)。在胃肠肿瘤患者中,游离上清中TS m RNA表达水平与5-FU药物敏感性相关(P=0.002)。尽管观察到游离上清中β-tubulinⅢm RNA高表达者多西紫杉醇药物敏感性高的趋势,但差异没有统计学意义。结论:晚期肿瘤患者恶性胸腹水游离上清中可检测到游离TS、β-tubulin III m RNA的表达。在胃肠肿瘤患者中,游离上清TS m RNA表达水平与5-FU药物敏感相关。展开更多
目的筛选并分析孕中期羊水游离RNA(AfcfRNA)中的泌尿系统发育关键基因。方法从GEO数据库获取胎儿AfcfRNA的芯片检测数据。对56例AfcfRNA的芯片检测结果进行基因共表达网络(WGCNA)分析,建立共表达网络模块。从Human Protein Atlas数据库...目的筛选并分析孕中期羊水游离RNA(AfcfRNA)中的泌尿系统发育关键基因。方法从GEO数据库获取胎儿AfcfRNA的芯片检测数据。对56例AfcfRNA的芯片检测结果进行基因共表达网络(WGCNA)分析,建立共表达网络模块。从Human Protein Atlas数据库中筛选在泌尿组织中表达量高于平均表达量10倍的基因为泌尿组织特异基因。以泌尿组织特异基因和共表达模块为基础,建立泌尿系统特异基因共表达模块。对泌尿组织特异共表达模块中的基因进行GO分析,阐述其生物学功能。利用STRING数据库进行基因编码蛋白的蛋白-蛋白相互作用网络分析,设定combine_score为150、degree为20,并删除表达量偏低(<P25)的基因,筛选得到泌尿系统发育关键基因。结果通过WGCNA分析共建立20个共表达模块,在Human Protein Atlas数据库中筛选到270个泌尿组织特异基因。brown、green及turquoise模块中的基因与中肾发生和发育、肾泡的发育、肾单位发生、肾小管生成、肾脏发育及泌尿生殖系统发生密切相关。共筛选出13个泌尿系统发育关键基因,其中brown模块5个(CLCNKB、SLC6A18、NAT8、MIOX及SLC3A1)、turquoise模块8个(AQP2、CYP4A11、SLC7A9、SLC13A3、FXYD2、TRPV5、SLC17A1及BSND)。结论获得13个与泌尿系统发育密切相关的关键基因,基因功能涉及中肾发生和发育、肾泡的发育、肾单位发生、肾小管生成、肾脏发育及泌尿生殖系统发生等。这些关键基因有望成为产前诊断中评估泌尿系统发育的标志物。展开更多
Hepatocellular carcinoma(HCC)is one of the most common malignant tumors worldwide and is associated with high mortality.The currently used methods for diagnosing HCC,including imaging modalities and liver biopsy,detec...Hepatocellular carcinoma(HCC)is one of the most common malignant tumors worldwide and is associated with high mortality.The currently used methods for diagnosing HCC,including imaging modalities and liver biopsy,detect tumors at a relatively advanced stage or are invasive.Non-invasive biomarkers are urgently needed to facilitate screening and early diagnosis of HCC,as well as treatment monitoring and detection of tumor recurrence.Liquid biopsy,the analysis of blood or other body fluids to obtain genetic and epigenetic information,has historically been applied to other types of cancer including breast and prostate cancer.Over the past few decades,liquid biopsy analysis has shed significant insights on genetic and epigenetic aberrations in HCC detectable in peripheral blood.Aberrations in nucleic acids found circulating freely in body fluids or contained within extracellular vesicles such as exosomes or microvesicles show potential clinical utility as non-invasive biomarkers.In this review,we present available literature on cell-free nucleic acids in the diagnosis of HCC.展开更多
文摘Despite recent advances in surgical techniques and perioperative management, the prognosis of pancreatic cancer(PCa) remains extremely poor. To provide optimal treatment for each patient with Pca, superior biomarkers are urgently needed in all phases of management from early detection to staging, treatment monitoring, and prognosis. In the blood of patients with cancer, circulating tumor cells(CTCs) and cell-free nucleic acids(cf NAs), such as DNA, m RNA, and noncoding RNA have been recognized. In the recent years, their presence in the blood has encouraged researchers to investigate their potential use as novel blood biomarkers, and numerous studies have demonstrated their potential clinical utility as a biomarker for certain types of cancer. This concept, called "liquid biopsy" has been focused on as a less invasive, alternative approach to cancer tissue biopsy for obtaining genetic and epigenetic aberrations that contribute to oncogenesis and cancer progression. In this article, we review the available literature on CTCs and cfN As in patients with cancer, particularly focusing on PCa, and discuss future perspectives in this field.
文摘目的 :探索恶性胸腹水上清中游离胸苷酸合成酶(TS)、β-tubulinⅢm RNA的表达,以及游离TS、β-tubulinⅢ表达和患者5-氟尿嘧啶(5-FU)、多西紫杉醇药物敏感性的关系。方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离、提取游离核酸,分离肿瘤细胞。实时荧光定量RT-PCR分别检测游离上清和肿瘤细胞中TS和β-tubulinⅢm RNA表达;ATP-TCA方法检测肿瘤细胞5-FU、多西紫杉醇的敏感性。结果:TS和β-tubulinⅢm RNA在恶性胸/腹水游离上清中的检出率为98%。与配对的肿瘤细胞相比,游离上清中TS m RNA表达水平高(P=0.02)。在胃肠肿瘤患者中,游离上清中TS m RNA表达水平与5-FU药物敏感性相关(P=0.002)。尽管观察到游离上清中β-tubulinⅢm RNA高表达者多西紫杉醇药物敏感性高的趋势,但差异没有统计学意义。结论:晚期肿瘤患者恶性胸腹水游离上清中可检测到游离TS、β-tubulin III m RNA的表达。在胃肠肿瘤患者中,游离上清TS m RNA表达水平与5-FU药物敏感相关。
文摘目的筛选并分析孕中期羊水游离RNA(AfcfRNA)中的泌尿系统发育关键基因。方法从GEO数据库获取胎儿AfcfRNA的芯片检测数据。对56例AfcfRNA的芯片检测结果进行基因共表达网络(WGCNA)分析,建立共表达网络模块。从Human Protein Atlas数据库中筛选在泌尿组织中表达量高于平均表达量10倍的基因为泌尿组织特异基因。以泌尿组织特异基因和共表达模块为基础,建立泌尿系统特异基因共表达模块。对泌尿组织特异共表达模块中的基因进行GO分析,阐述其生物学功能。利用STRING数据库进行基因编码蛋白的蛋白-蛋白相互作用网络分析,设定combine_score为150、degree为20,并删除表达量偏低(<P25)的基因,筛选得到泌尿系统发育关键基因。结果通过WGCNA分析共建立20个共表达模块,在Human Protein Atlas数据库中筛选到270个泌尿组织特异基因。brown、green及turquoise模块中的基因与中肾发生和发育、肾泡的发育、肾单位发生、肾小管生成、肾脏发育及泌尿生殖系统发生密切相关。共筛选出13个泌尿系统发育关键基因,其中brown模块5个(CLCNKB、SLC6A18、NAT8、MIOX及SLC3A1)、turquoise模块8个(AQP2、CYP4A11、SLC7A9、SLC13A3、FXYD2、TRPV5、SLC17A1及BSND)。结论获得13个与泌尿系统发育密切相关的关键基因,基因功能涉及中肾发生和发育、肾泡的发育、肾单位发生、肾小管生成、肾脏发育及泌尿生殖系统发生等。这些关键基因有望成为产前诊断中评估泌尿系统发育的标志物。
基金This work was supported by the NIH Grants(5T32 DK07150,RO1 DK 10596).
文摘Hepatocellular carcinoma(HCC)is one of the most common malignant tumors worldwide and is associated with high mortality.The currently used methods for diagnosing HCC,including imaging modalities and liver biopsy,detect tumors at a relatively advanced stage or are invasive.Non-invasive biomarkers are urgently needed to facilitate screening and early diagnosis of HCC,as well as treatment monitoring and detection of tumor recurrence.Liquid biopsy,the analysis of blood or other body fluids to obtain genetic and epigenetic information,has historically been applied to other types of cancer including breast and prostate cancer.Over the past few decades,liquid biopsy analysis has shed significant insights on genetic and epigenetic aberrations in HCC detectable in peripheral blood.Aberrations in nucleic acids found circulating freely in body fluids or contained within extracellular vesicles such as exosomes or microvesicles show potential clinical utility as non-invasive biomarkers.In this review,we present available literature on cell-free nucleic acids in the diagnosis of HCC.
