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A new level set model for cell image segmentation 被引量:4
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作者 马竟锋 侯凯 +1 位作者 包尚联 陈纯 《Chinese Physics B》 SCIE EI CAS CSCD 2011年第2期568-574,共7页
In this paper we first determine three phases of cell images: background, cytoplasm and nucleolus according to the general physical characteristics of cell images, and then develop a variational model, based on these... In this paper we first determine three phases of cell images: background, cytoplasm and nucleolus according to the general physical characteristics of cell images, and then develop a variational model, based on these characteristics, to segment nucleolus and cytoplasm from their relatively complicated backgrounds. In the meantime, the preprocessing obtained information of cell images using the OTSU algorithm is used to initialize the level set function in the model, which can speed up the segmentation and present satisfactory results in cell image processing. 展开更多
关键词 cell image segmentation 3-phase level set OTSU algorithm
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Photovoltaic Cell Panels Soiling Inspection Using Principal Component Thermal Image Processing
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作者 A.Sriram T.D.Sudhakar 《Computer Systems Science & Engineering》 SCIE EI 2023年第6期2761-2772,共12页
Intended for good productivity and perfect operation of the solar power grid a failure-free system is required.Therefore,thermal image processing with the thermal camera is the latest non-invasive(without manual conta... Intended for good productivity and perfect operation of the solar power grid a failure-free system is required.Therefore,thermal image processing with the thermal camera is the latest non-invasive(without manual contact)type fault identification technique which may give good precision in all aspects.The soiling issue,which is major productivity affecting factor may import from several reasons such as dust on the wind,bird mucks,etc.The efficient power production sufferers due to accumulated soil deposits reaching from 1%–7%in the county,such as India,to more than 25%in middle-east countries country,such as Dubai,Kuwait,etc.This research offers a solar panel soiling detection system built on thermal imaging which powers the inspection method and mitigates the requirement for physical panel inspection in a large solar production place.Hence,in this method,solar panels can be verified by working without disturbing production operation and it will save time and price of recognition.India ranks 3rd worldwide in the usage use age of Photovoltaic(PV)panels now and it is supported about 8.6%of the Nation’s electricity need in the year 2020.In the meantime,the installed PV production areas in India are aged 4–5 years old.Hence the need for inspection and maintenance of installed PV is growing fast day by day.As a result,this research focuses on finding the soiling hotspot exactly of the working solar panels with the help of Principal Components Thermal Analysis(PCTA)on MATLAB Environment. 展开更多
关键词 PV cell thermal imaging PCTA(Principal Components Thermal Analysis) PV cell soiling detection
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Multiplexed stimulated emission depletion nanoscopy(mSTED)for 5-color live-cell long-term imaging of organelle interactome
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作者 Yuran Huang Zhimin Zhang +9 位作者 Wenli Tao Yunfei Wei Liang Xu Wenwen Gong Jiaqiang Zhou Liangcai Cao Yong Liu Yubing Han Cuifang Kuang Xu Liu 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第7期17-26,共10页
Stimulated emission depletion microscopy(STED)holds great potential in biological science applications,especially in studying nanoscale subcellular structures.However,multi-color STED imaging in live-cell remains chal... Stimulated emission depletion microscopy(STED)holds great potential in biological science applications,especially in studying nanoscale subcellular structures.However,multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser radiation.Here,we develop a multiplexed live-cell STED method to observe more structures simultaneously with limited photo-bleaching and photo-cytotoxicity.By separating live-cell fluorescent probes with similar spectral properties using phasor analysis,our method enables five-color live-cell STED imaging and reveals long-term interactions between different subcellular structures.The results here provide an avenue for understanding the complex and delicate interactome of subcellular structures in live-cell. 展开更多
关键词 optical nanoscopy phasor analysis multicolor live cell imaging
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Application of Image Fusion Methods to Cell Imaging Processing
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作者 李勤 代彩虹 +4 位作者 俞信 王苏生 张同存 曹恩华 李景福 《Journal of Beijing Institute of Technology》 EI CAS 1998年第4期412-417,共6页
Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imag... Aim To fuse the fluorescence image and transmission image of a cell into a single image containing more information than any of the individual image. Methods Image fusion technology was applied to biological cell imaging processing. It could match the images and improve the confidence and spatial resolution of the images. Using two algorithms, double thresholds algorithm and denoising algorithm based on wavelet transform,the fluorescence image and transmission image of a Cell were merged into a composite image. Results and Conclusion The position of fluorescence and the structure of cell can be displyed in the composite image. The signal-to-noise ratio of the exultant image is improved to a large extent. The algorithms are not only useful to investigate the fluorescence and transmission images, but also suitable to observing two or more fluoascent label proes in a single cell. 展开更多
关键词 image fusion wavelet transform double thresholds algorithm denoising algorithms living cell image
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Recent advances in optical techniques for dynamically probing cellular mechanobiology
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作者 Fengqi Wang Qin Zhang +2 位作者 Mo Yang Bohan Yin Siu Hong Dexter Wong 《Biomedical Engineering Communications》 2024年第3期3-11,共9页
Cellular mechanotransduction characterized by the transformation of mechanical stimuli into biochemical signals,represents a pivotal and complex process underpinning a multitude of cellular functionalities.This proces... Cellular mechanotransduction characterized by the transformation of mechanical stimuli into biochemical signals,represents a pivotal and complex process underpinning a multitude of cellular functionalities.This process is integral to diverse biological phenomena,including embryonic development,cell migration,tissue regeneration,and disease pathology,particularly in the context of cancer metastasis and cardiovascular diseases.Despite the profound biological and clinical significance of mechanotransduction,our understanding of this complex process remains incomplete.The recent development of advanced optical techniques enables in-situ force measurement and subcellular manipulation from the outer cell membrane to the organelles inside a cell.In this review,we delved into the current state-of-the-art techniques utilized to probe cellular mechanobiology,their principles,applications,and limitations.We mainly examined optical methodologies to quantitatively measure the mechanical properties of cells during intracellular transport,cell adhesion,and migration.We provided an introductory overview of various conventional and optical-based techniques for probing cellular mechanics.These techniques have provided into the dynamics of mechanobiology,their potential to unravel mechanistic intricacies and implications for therapeutic intervention. 展开更多
关键词 MECHANOBIOLOGY cell adhesion optical techniques live cell imaging cell fates
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Simultaneous Morphologies and Luminescence Control of NaYF_(4)∶Yb/Er Nanophosphors by Surfactants for Cancer Cell Imaging
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作者 盛洋怡 程璐 +3 位作者 宋岳林 王兆洁 蒋伟忠 陈志钢 《Journal of Donghua University(English Edition)》 CAS 2023年第2期127-133,共7页
Hydrophilic rare-earth up-conversion nanophosphors(UCNPs)with small sizes and a strong up-conversion luminescence have attracted much interest.Herein the simultaneous control of morphologies and the up-conversion lumi... Hydrophilic rare-earth up-conversion nanophosphors(UCNPs)with small sizes and a strong up-conversion luminescence have attracted much interest.Herein the simultaneous control of morphologies and the up-conversion luminescence intensities was reported for NaYF_(4)∶Yb/Er nanophosphors by a facile hydrothermal procedure with different surfactants.With the change of the surfactants from polyvinylpyrrolidone(PVP)to sodium citrate(CIT),edetate disodium(EDTA)or sodium dodecyl benzenesulfonate(SDBS),the morphology of NaYF_(4)∶Yb/Er nanophosphors transformed from nanoparticles with a diameter of about 70.0 nm to hexagonal nanoblocks with a thickness of about 125.0 nm and a length of about 240.0 nm,nanorods with a diameter of about 700.0 nm and a length of about 2.6μm,or nanowires with a diameter of 250.0 nm and a length of about 3.2μm.Simultaneously,their up-conversion luminescence intensity went down gradually under laser irradiation at a wavelength of 980 nm due to the increase of photobleaching.PVP-capped NaYF_(4)∶Yb/Er nanoparticles exhibited the smallest size and the strongest up-conversion luminescence intensity.Biological experiment results revealed that NaYF_(4)∶Yb/Er nanophosphors exhibited a high biocompatibility and could be used as biological labels with a perfect signal-to-noise ratio for cancer cell imaging. 展开更多
关键词 NaYF_(4) NANOPHOSPHOR LUMINESCENCE surfactant adjustable morphology cancer cell imaging
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Three-dimensional structure of liver vessels and spatial distribution of hepatic immune cells
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作者 Mengli Xu Zheng Liu +4 位作者 Xinlin Li Xinru Wang Xuenan Yuan Chenlu Han Zhihong Zhang 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2023年第3期65-77,共13页
As the largest internal organ of the human body,the liver has an extremely complex vascularnetwork and multiple types of immune cells.It plays an important role in blood circulation,material metabolism,and immune resp... As the largest internal organ of the human body,the liver has an extremely complex vascularnetwork and multiple types of immune cells.It plays an important role in blood circulation,material metabolism,and immune response.Optical imaging is an effective tool for studying finevascular structure and immunocyte distribution of the liver.Here,we provide an overview of thestructure and composition of liver vessels,the threedimensional(3D)imaging of the liver,andthe spatial distribution and immune function of various cell components of the liver.Especially,we emphasize the 3D imaging methods for visualizing fine structure in the liver.Finally,wesummarize and prospect the development of 3D imaging of liver vesels and immune cells. 展开更多
关键词 LIVER blood vessel immune cell 3D imaging
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OBTAINING AND PR0CESSING OF DIFFRACTION ANDBACKSCATTERING IMAGE OF POLYDISPERSE RED BLOOD CELL
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《Chinese Journal of Biomedical Engineering(English Edition)》 1999年第4期96-97,共2页
关键词 PR OBTAINING AND PR0CESSING OF DIFFRACTION ANDBACKSCATTERING image OF POLYDISPERSE RED BLOOD cell
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Comparison of shape representation methods for dynamic cell analysis 被引量:1
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作者 李衡 刘志文 +1 位作者 安兴 时永刚 《Journal of Beijing Institute of Technology》 EI CAS 2014年第4期541-548,共8页
To evaluate the performance of basic shape representation methods for the description of dynamic cellular morphology, several frequently-used shape descriptors are compared. The methods are examined by using 50 lympho... To evaluate the performance of basic shape representation methods for the description of dynamic cellular morphology, several frequently-used shape descriptors are compared. The methods are examined by using 50 lymphocyte video clips including two kinds of lymphocyte cells. Our goal is to represent cell shape in each frame accurately, meanwhile precisely classify the two groups of cells based on the cellular morphological variations in the video clips. Experimental results illustrate that in general the region-based shape descriptors outperform the contour-based ones, since the contourbased methods are excessively sensitive and ignorant to cellular internal information. Due to their robustness to noise, the region-based shape descriptors are suitable for dynamic cell representation. Although region-based methods are more time-consuming, they analyze the entire cell area. 展开更多
关键词 cellular morphology dynamic cell images shape descriptors
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Automated Dynamic Cellular Analysis in Time-Lapse Microscopy
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作者 Shuntaro Aotake Chamidu Atupelage +3 位作者 Zicong Zhang Kota Aoki Hiroshi Nagahashi Daisuke Kiga 《Journal of Biosciences and Medicines》 2016年第3期44-50,共7页
Analysis of cellular behavior is significant for studying cell cycle and detecting anti-cancer drugs. It is a very difficult task for image processing to isolate individual cells in confocal microscopic images of non-... Analysis of cellular behavior is significant for studying cell cycle and detecting anti-cancer drugs. It is a very difficult task for image processing to isolate individual cells in confocal microscopic images of non-stained live cell cultures. Because these images do not have adequate textural variations. Manual cell segmentation requires massive labor and is a time consuming process. This paper describes an automated cell segmentation method for localizing the cells of Chinese hamster ovary cell culture. Several kinds of high-dimensional feature descriptors, K-means clustering method and Chan-Vese model-based level set are used to extract the cellular regions. The region extracted are used to classify phases in cell cycle. The segmentation results were experimentally assessed. As a result, the proposed method proved to be significant for cell isolation. In the evaluation experiments, we constructed a database of Chinese Hamster Ovary Cell’s microscopic images which includes various photographing environments under the guidance of a biologist. 展开更多
关键词 High Dimension Feature Analysis Microscopic cell image cell Division Cycle Identification Active Contour Model K-Means Clustering
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Synthesis of Fluorescent Carbon Quantum Dots and Their Application in the Plant Cell Imaging 被引量:2
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作者 DING Liyun LI Junli 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2018年第6期1546-1550,共5页
Carbon quantum dots(CQDs) exhibit tremendous advantages for plant growth study due to its strong fluorescence and good biocompatibility. The fluorescent CQDs were synthesized by the onestep microwave method with the r... Carbon quantum dots(CQDs) exhibit tremendous advantages for plant growth study due to its strong fluorescence and good biocompatibility. The fluorescent CQDs were synthesized by the onestep microwave method with the raw materials of citric acid(CA) and urea(UR), and expressed a unique green fluorescence with the optimal excitation wavelength of over 400 nm through adjusting the doping of N elements. It is demonstrated that CQDs can act as deliver media in plant and fluorescent probes for plant cell imaging through directly cultivated in the seedlings of melon and wheat, respectively. Based on the effects of the fluorescent CQDs on plants growth, we can further study the mechanisms of the ions transport in plants. 展开更多
关键词 carbon quantum dots plant cell imaging microwave method
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Tracking of iron-labeled human neural stem cells by magnetic resonance imaging in cell replacement therapy for Parkinson's disease 被引量:5
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作者 Milagros Ramos-Gómez Alberto Martínez-Serrano 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第1期49-52,共4页
Human neural stem cells(h NSCs) derived from the ventral mesencephalon are powerful research tools and candidates for cell therapies in Parkinson's disease. However, their clinical translation has not been fully re... Human neural stem cells(h NSCs) derived from the ventral mesencephalon are powerful research tools and candidates for cell therapies in Parkinson's disease. However, their clinical translation has not been fully realized due, in part, to the limited ability to track stem cell regional localization and survival over long periods of time after in vivo transplantation. Magnetic resonance imaging provides an excellent non-invasive method to study the fate of transplanted cells in vivo. For magnetic resonance imaging cell tracking, cells need to be labeled with a contrast agent, such as magnetic nanoparticles, at a concentration high enough to be easily detected by magnetic resonance imaging. Grafting of human neural stem cells labeled with magnetic nanoparticles allows cell tracking by magnetic resonance imaging without impairment of cell survival, proliferation, self-renewal, and multipotency. However, the results reviewed here suggest that in long term grafting, activated microglia and macrophages could contribute to magnetic resonance imaging signal by engulfing dead labeled cells or iron nanoparticles dispersed freely in the brain parenchyma over time. 展开更多
关键词 human neural stem cells Parkinson's disease magnetic resonance imaging magnetic nanoparticles stem cell transplantation
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In vivo bioluminescence imaging of hyperglycemia exacerbating stem cells on choroidal neovascularization in mice 被引量:2
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作者 Xiang Gao Yu Wang +6 位作者 Hui-Yuan Hou Yang Lyu Hai-Yan Wang Li-Bo Yao Jian Zhang Feng Cao Yu-Sheng Wang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第4期519-527,共9页
AIM: To investigate the influence of hyperglycemia on the severity of choroidal neovascularization(CNV),especially the involvement of bone marrow-derived cells(BMCs) and underlying mechanisms.·METHODS: BMCs... AIM: To investigate the influence of hyperglycemia on the severity of choroidal neovascularization(CNV),especially the involvement of bone marrow-derived cells(BMCs) and underlying mechanisms.·METHODS: BMCs from firefly luciferase(Fluc)/green fluorescent protein(GFP) double transgenic mice were transplanted into C57BL/6J wide-type mice. The recipient mice were injected intraperitoneally with streptozotocin(STZ) daily for 5 consecutive days to induce diabetes mellitus(DM), followed by CNV laser photocoagulation.The BMCs recruitment in CNV exposed to hyperglycemia was firstly examined in Fluc/GFP chimeric mice by in vivo optical bioluminescence imaging(BLI) and in vitro Fluc assays. The CNV severity was evaluated by H&E staining and choroidal flatmount. The expression of vascular endothelial growth factor(VEGF) and stromal cell derived factor-1(SDF-1) was detected by Western blot.·RESULTS: BLI showed that the BMCs exerted dynamic effects in CNV model in Fluc/GFP chimeric mice exposed to hyperglycemia. The signal intensity of transplanted Fluc+GFP+BMCs in the DM chimeric mice was significantly higher than that in the control chimeric mice with CNV induction at days 5, 7, 14 and 21(121861.67 ±9948.81 vs 144998.33 ±13787.13 photons/second/cm2/sr for control and DM mice, P5d〈0.05; 178791.67±30350.8 vs240166.67 ±22605.3, P7d〈0.05; 124176.67 ±16253.52 vs196376.67 ±18556.79, P14d〈0.05; 97951.60 ±10343.09 vs119510.00 ±14383.76, P21d〈0.05), which was consistent with in vitro Fluc assay at day 7 [relative light units of Fluc(RLU1)], 215.00±52.05 vs 707.33±88.65, P 〈0.05; RLU1/relative light units of renilla luciferase(RLU2), 0.90 ±0.17 vs 1.83 ±0.17, P 〈0.05]. The CNVs in the DM mice were wider than those in the control group at days 5, 7, 14 and21(147.83±17.36 vs 220.33±20.17 μm, P5d〈0.05; 212.17 ±24.63 vs 326.83 ±19.49, P7d〈0.05; 163.17 ±18.24 vs265.17 ±20.55, P14d〈0.05; 132.00 ±10.88 vs 205.33 ±12.98,P21d〈0.05). The average area of CNV in the DM group was larger at 7d(20688.67±3644.96 vs 32218.00±4132.69 μm2,P 〈0.05). The expression of VEGF and SDF-1 was enhanced in the DM mice.·CONCLUSION: Hyperglycemia promots the vasculo-genesis of CNV, especially the contribution of BMCs,which might be triggered by VEGF and SDF-1 production. 展开更多
关键词 hyperglycemia choroidal neovascularization bone marrow-derived cells molecular imaging in vivo optical bioluminescence imaging
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Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells
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作者 Xin-Yuan Hu Jia-Jing Li +2 位作者 Zi-Wei Yang Jun Zhang Huai-Song Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2022年第5期801-807,共7页
The catalytic generation of H_(2) in living cells provides a method for antioxidant therapy.In this study,an[FeFe]-hydrogenase mimic[Ru+Fe_(2)S_(2)@F127(80)]was synthesized by self-assembling polymeric pluronic F-127,... The catalytic generation of H_(2) in living cells provides a method for antioxidant therapy.In this study,an[FeFe]-hydrogenase mimic[Ru+Fe_(2)S_(2)@F127(80)]was synthesized by self-assembling polymeric pluronic F-127,catalytic[Fe_(2)S_(2)]sites,and photosensitizer Ru(bpy)_(3)^(2+).Under blue light irradiation,hydrated protons were photochemically reduced to H2,which increased the local pH in living cells(HeLa cells).The generated H2 was subsequently used as an antioxidant to decrease reactive oxygen species(ROS)levels in living cells(HEK 293T,HepG2,MCF-7,and HeLa cells).Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times,relative to that of other cells(HepG2,MCF-7,and HeLa cells).Intracellular ROS and pH levels were then monitored using fluorescent cell imaging.Our study showed that cell imaging can be used to evaluate the ability of Ru t Fe2S2@F127 to eliminate oxidative stress and prevent ROS-related diseases. 展开更多
关键词 cell imaging [FeFe]-hydrogenase Reactive oxygen species Photocatalytic system
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Live imaging of the effects of fucoidan on cell proliferation for laboratory instruction
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作者 Hong Wu 《TMR Theory and Hypothesis》 2018年第2期45-50,共6页
The aim of this study is to introduce live cell imaging and its applications for the evaluation of the effects of fucoidan, a fucose-enriched sulfated polysaccharide, on the proliferation of cultured cells in vitro. I... The aim of this study is to introduce live cell imaging and its applications for the evaluation of the effects of fucoidan, a fucose-enriched sulfated polysaccharide, on the proliferation of cultured cells in vitro. In this study, long-term time- lapse observation (87 h) of the effects of fucoidan was conducted using BioStation CT, an integrated cell culture observation system. In contrast, the effects of heparin, which has a similar structure to fucoidan, were observed to distinguish the differences between the two chemicals. At the same time, the viability of the floating cells detached by fucoidan in the medium was measured by culturing them again in the absence of fucoidan. Finally, total internal reflection fluorescence microscopy (TIRF) was used to confirm when the detachment of the cells by fucoidan occurred. The results indicate that the inhibitory effects of fucoidan on the proliferation of cells are dose-dependent (from 0.125 mg/ml to 1.0 mg/ml). Fucoidan also causes cell detachment without killing all the cells within 24 hours. The cell detachment did not occur until after half an hour, as observed under the TIRF microscope. Combined with our previous study, the findings suggest that the inhibition of calcium responses by fucoidan may be one of the mechanisms underlying its inhibition of cell proliferation, which is responsible for the death of cancer cells. Cell proliferation can be visualized in the real time and the images can provide important information regarding when and how the cells grow and proliferate. 展开更多
关键词 Live cell imaging TIME-LAPSE cell proliferation FUCOIDAN
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A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells
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作者 Chuan-Zhi Ni Ruo-Ming Li +4 位作者 Fang-Qi Zhang Qu-Ao-Wei Li Yuan-Yuan Zhu Jie Zeng Shuang-Xi Gu 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第10期364-369,共6页
A novel and readily available binaphthyl-based fluorescent probe(S)-1 was designed and synthesized.(S)-1 can be used to not only chemoselectively discriminate 3 basic amino acids out of common amino acids,but also ena... A novel and readily available binaphthyl-based fluorescent probe(S)-1 was designed and synthesized.(S)-1 can be used to not only chemoselectively discriminate 3 basic amino acids out of common amino acids,but also enantioselectively recognize histidine.Encouragingly,enantioselective imaging of histidine in cells was achieved for the first time by the probe(S)-1.These performances endowed it potential application in the chiral analysis of basic amino acids in asymmetric synthesis and cell imaging for diagnosis of diseases caused by racemization of histidine.Nuclear magnetic resonance(NMR)and mass spectrometry investigations suggested that different reaction extent of(S)-1 with L/D-histidine and different product structures generated the observed enantioselective fluorescent response.The molecular structures and thermodynamic stability of the complexes,formed from(S)-1+Zn2+and enantiomers of histidine,were calculated by Gaussian 16 based on density functional theory(DFT)to validate the above action mechanism. 展开更多
关键词 Fluorescent probe Chiral recognition ENANTIOSELECTIVITY CHEMOSELECTIVITY Basic amino acids cell imaging
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Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging
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作者 Boran Cheng Lei Cao +7 位作者 Chen Li Fang-Yi Huo Qian-Fang Meng Ganglin Tong Xuan Wu Lin-Lin Bu Lang Rao Shubin Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第6期433-438,共6页
As a type of new carbon-based nanomaterials,carbon dots(CDs)possess exceptional optical properties,making them highly desirable for use in fluorescent sensors.However,the CDs with deep-red(DR)or near-infrared(NIR)emis... As a type of new carbon-based nanomaterials,carbon dots(CDs)possess exceptional optical properties,making them highly desirable for use in fluorescent sensors.However,the CDs with deep-red(DR)or near-infrared(NIR)emission have rarely been reported.In this work,we prepared deep-red emissive fluorine-doped carbon quantum dots(F-CDs)by introducing a precursor simultaneously containing fluorine and amidogen.The synergistic effect of nitrogen doping and D-π-A pattern production contributed to the maximum emission of F-CDs at 636 nm with an absolute quantum yield of 36.00%±0.68%.Moreover,we designed an F-CDs-based fluorescence assay to determine the content of hypochlorite(ClO^(-)),with a limit of detection(LOD)as low as 15.4 nmol/L,indicating the high sensitivity of F-CDs to ClO^(-).In real samples,the F-CDs-based fluorescent sensor exhibited excellent sensitivity and selectivity in the detection of ClO^(-),with an error below 2%,suggesting their great potential in daily life.In cancer cell imaging,the F-CDs not only demonstrated high sensitivity to ClO^(-)but also exhibited excellent mitochondria targeting,as evidenced by the high Pearson's correlation coefficient(PCC)of 0.93 in colocalization analysis.The work presented here suggests the great potential of replacing commercial dyes with F-CDs for highly specific mitochondria labeling and cell imaging. 展开更多
关键词 Carbon dots Near-infrared cell imaging Mitochondria labeling Hypochlorite determination
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Dynamic Measurement of Intracellular pH Based on Bioluminescent Bacteria
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作者 LI Yaohua WANG Wei 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2024年第2期287-292,共6页
Intracellular pH(pHi)is a fundamental indicator of cellular physiological state,regulating cellular state and function,and has important research values.Although various probes for measuring intracellular pH were avai... Intracellular pH(pHi)is a fundamental indicator of cellular physiological state,regulating cellular state and function,and has important research values.Although various probes for measuring intracellular pH were available,it is challenging to reflect pHi in real-time and reversible manners.Herein,we developed a whole-cell bioluminescent(BL)probe based on wild type BL bacteria,photobacterium phosphoreum(P.phosphoreum),to determine and image pHi.The dependence of BL intensity of P.phosphoreum on pH values of culture solutions was established.It was found that BL intensity could respond to the change of pH values rapidly and reversibly.We further revealed that P.phosphoreum maintained pH homeostasis in the extracellular pH(pHe)within the range of 5.0–7.0,while intracellular pH homeostasis was destroyed at the alkaline pHe.This method opens up the enormous potential of BL bacteria as an alternative to fluorescence for monitoring and imaging pHi. 展开更多
关键词 BIOLUMINESCENCE P.phosphoreum Intracellular pH cell imaging
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Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging
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作者 Zhixue Liu Haiqi Chen +5 位作者 Lijuan Guo Xinyao Sun Zhi-Yuan Zhang Junyi Chen Ming Dong Chunju Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第9期183-186,共4页
Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and functions.Herein,we reported macrocyclic supramolecular assemblies based on lumin... Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and functions.Herein,we reported macrocyclic supramolecular assemblies based on luminescent terphen[3]arene sulfate(TP[3]AS)and tetraphenylethylene pyridinium(TPE-4Py)through electrostatic interactions,host-guest encapsulation andπ-πstacking interactions.F?rster resonance energy transfer(FRET)process from TP[3]AS to TPE-4Py was achieved with the energy transfer efficiency of 99.9%,accompanied by TPE-4Py fluorescence emission bathochromic shifted of 15 nm and enhanced by 1.68 times in PBS solution.In contrast,other non-luminescent sulfato-β-cyclodextrin and sulfobutylether-β-cyclodextrin only can enhance the fluorescence intensity of TPE-4Py without bathochromic shift.Due to the strong fluorescence and good stability of TPE-4Py@TP[3]AS,it can be used for optical imaging in living cells,which provided an effective approach for the construction of assembling-confined luminescent biomaterials. 展开更多
关键词 Luminescent macrocycle arene sulfate Multicharged assembly Multivalent interactions Forster resonance energy transfer cell imaging
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New Opportunities for Lanthanide Luminescence 被引量:32
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作者 Jean-Claude G. Bünzli Steve Comby Anne-Sophie Chauvin Caroline D. B. Vandevyver 《Journal of Rare Earths》 SCIE EI CAS CSCD 2007年第3期257-274,共18页
Trivalent lanthanide ions display fascinating optical properties. The discovery of the corresponding elements and their first industrial uses were intimately linked to their optical properties. This relationship has b... Trivalent lanthanide ions display fascinating optical properties. The discovery of the corresponding elements and their first industrial uses were intimately linked to their optical properties. This relationship has been kept alive until today when many high-technology applications of lanthanide-containing materials such as energy-saving lighting devices, displays, optical fibers and amplifiers, lasers, responsive luminescent stains for biomedical analyses and in cellulo sensing and imaging, heavily rely on the brilliant and pure-color emission of lanthanide ions. In this review we first outlined the basics of lanthanide luminescence with emphasis on f-f transitions, the sensitization mechanisms, and the assessment of the luminescence efficiency of lanthanide-containing emissive molecular edifices. Emphasis was then put on two fast developing aspects of lanthanide luminescence: materials for telecommunications and light emitting diodes, and biomedical imaging and sensing. Recent advances in NIR-emitting materials for plastic amplifiers and waveguides were described, together with the main solutions brought by researchers to minimize non-radiative deactivation of excited states. The demonstration in 1999 that erbium tris(8-hydroxyquinolinate) displayed a bright green emission suitable for organic light emitting diodes (OLEDs) was followed by realizing that in OLEDs, 25% of the excitation energy leads to singlet states and 75% to triplet states. Since lanthanide ions are good triplet quenchers, they now also play a key role in the development of these lighting devices. Luminescence analyses of biological molecules are among the most sensitive analytical techniques known. The long lifetime of the lanthanide excited states allows time-resolved spectroscopy to be used, suppressing the sample autofluorescence and reaching very low detection limits. Not only visible lanthanide sensors are now ubiquitously provided in medical diagnosis and in cell imaging, but the feasibility of using NIR emission of ions such as YbⅢ is now being tested because of deeper penetration in biological tissues. 展开更多
关键词 lanthanide luminescence SENSITIZATION near infrared telecommunications organic light emitting diode (OLED) time-resolved luminescence in cellulo sensing cell imaging rare earths
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