Resurrection fern has a unique ability to maintain cell wall integrity when the plant cell is desiccated. It uses proteins such as late embryogenesis proteins and heat shock proteins to maintain their cellular functio...Resurrection fern has a unique ability to maintain cell wall integrity when the plant cell is desiccated. It uses proteins such as late embryogenesis proteins and heat shock proteins to maintain their cellular functions. The purpose of this experiment is to determine the effects of environmental stressors on the physiological response of the resurrection fern (Pleopeltis polypodioides). The physiological response of resurrection fern plants was subjected to various temperatures (-50°C, 0°C, 25°C, and 50°C) for 7 days. Results indicated that there was a significant difference between hydrated and desiccated ferns based on the temperature. Additionally, electrolyte leakage measurements confirmed cell damage following exposure to temperature extremes of -50°C and 50°C.展开更多
在同轴圆筒装置的均匀剪切场中研究了不同剪切应力(0.16,0.46,0.78Pa)对悬浮培养的南方红豆杉细胞生理的短期影响.结果表明剪切应力为0.16～0.46Pa时,有利于细胞的生长和初生代谢;剪切应力为0 46～0.78Pa时,有利于细胞的次生代谢:另一...在同轴圆筒装置的均匀剪切场中研究了不同剪切应力(0.16,0.46,0.78Pa)对悬浮培养的南方红豆杉细胞生理的短期影响.结果表明剪切应力为0.16～0.46Pa时,有利于细胞的生长和初生代谢;剪切应力为0 46～0.78Pa时,有利于细胞的次生代谢:另一方面利用计算流体力学(CFD)对20L生物搅拌釜(45°-斜向上桨(45°-PBT),桨距为(1/3)T的流场进行了三维模拟,获得搅拌釜内部的剪切场.结果表明搅拌釜内剪切应力分布极不均匀,且主要集中在搅拌桨区和尾流区,约为主流区和挡板区值的3～5倍,有时甚至高达7倍.通过将实验结果和CFD模拟相结合,预测了20L生物搅拌釜剪切应力对南方红豆杉细胞生理的影响.表明当搅拌转速小于150 r min-1时,搅拌釜内相应的剪切应力有利于细胞的生长和初生代谢;而当搅拌转速大于200 r min-1时,则不宜于南方红豆杉细胞的培养.展开更多
文摘Resurrection fern has a unique ability to maintain cell wall integrity when the plant cell is desiccated. It uses proteins such as late embryogenesis proteins and heat shock proteins to maintain their cellular functions. The purpose of this experiment is to determine the effects of environmental stressors on the physiological response of the resurrection fern (Pleopeltis polypodioides). The physiological response of resurrection fern plants was subjected to various temperatures (-50°C, 0°C, 25°C, and 50°C) for 7 days. Results indicated that there was a significant difference between hydrated and desiccated ferns based on the temperature. Additionally, electrolyte leakage measurements confirmed cell damage following exposure to temperature extremes of -50°C and 50°C.
文摘在同轴圆筒装置的均匀剪切场中研究了不同剪切应力(0.16,0.46,0.78Pa)对悬浮培养的南方红豆杉细胞生理的短期影响.结果表明剪切应力为0.16～0.46Pa时,有利于细胞的生长和初生代谢;剪切应力为0 46～0.78Pa时,有利于细胞的次生代谢:另一方面利用计算流体力学(CFD)对20L生物搅拌釜(45°-斜向上桨(45°-PBT),桨距为(1/3)T的流场进行了三维模拟,获得搅拌釜内部的剪切场.结果表明搅拌釜内剪切应力分布极不均匀,且主要集中在搅拌桨区和尾流区,约为主流区和挡板区值的3～5倍,有时甚至高达7倍.通过将实验结果和CFD模拟相结合,预测了20L生物搅拌釜剪切应力对南方红豆杉细胞生理的影响.表明当搅拌转速小于150 r min-1时,搅拌釜内相应的剪切应力有利于细胞的生长和初生代谢;而当搅拌转速大于200 r min-1时,则不宜于南方红豆杉细胞的培养.
