Telomerase activity was found to be high in various human cancers, but absent in most normal tissues. Its expression pattern made it a novel target for antitumor agents. Several strategies against telomerase were pres...Telomerase activity was found to be high in various human cancers, but absent in most normal tissues. Its expression pattern made it a novel target for antitumor agents. Several strategies against telomerase were presented in this review. Targeting the telomerase RNA component by oligonucleotide/ribozyme was considered to be one of the most hopeful approaches. Some progresses were made in this area, such as the use of PANs and 2–5A antisense compounds. The relationships among telomerase activity and cell differentiation, signal transduction, oncogene, tumor suppressor gene as well as cell cycle modulation also provided a series of valuable ideas in designing anti-telomerase drugs for cancer therapy. In conclusion, although there is still a long way in understanding the mechanism and regulation of telomerase, the advance of studies on telomerase has allowed the development of numerous strategies for the treatment of cancer.展开更多
Objective： To investigate the mechanism of moxibustion in regulating cellular apoptosis in rat＇s precancerous lesion of primary hepatocellular carcinoma （HCC）. Methods： Seventy-four rats were randomly allocated t...Objective： To investigate the mechanism of moxibustion in regulating cellular apoptosis in rat＇s precancerous lesion of primary hepatocellular carcinoma （HCC）. Methods： Seventy-four rats were randomly allocated to normal group, model group and moxibustion group, and the diethylic nitrosamine （DEN） was used to establish HCC model. Moxibustion with moxa cone which is as big as a grain of wheat was performed on acupoint Zusanli （ST 36）, 3 cones for each acupoint and 0.5 mg for each cone, the treatment was given once a day, totally 16 weeks. Then the changes in the body weight, liver weight and thymus weight, a morphological change in the liver tissue and changes in γ-GT and GST were observed; Immunohistochemical staining method was adopted to observe the tendency of changes in relevant apoptosis genes such as C-myc, N-ras and mutant type P53, and the influence of moxibustion on cell cycle modulation genes such as cyclinD1, CDK4 and pl6. Results： Moxibustion could reduce the activities of γ-GT and GST in the blood, obviously decrease the protein expression of relevant apoptosis genes such as C-myc, N-ras and mutant type P53 and markedly inhibit the over-expression of relevant cell cycle modulation genes such as cyclinD1 and CDK4 and the mutation of cell cycle modulation gene pl 6. Conclusion： Moxibustion might play a certain role in relieving HCC precancerous lesion and its action mechanism might be related to the regulation on partial apoptosis genes.展开更多
CD44 is highly expressed in human acute myeloid leukemia （AML） cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indic...CD44 is highly expressed in human acute myeloid leukemia （AML） cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid （ATRA） and hexamethylene bisacetamide （HMBA）, but so far the mechanism was not demonstrated clearly. In the present study, we investigated whether ATRA or HMBA induced the growth arrest of HL-60 cells by down-regulating the expression of CD44. The results indicated that the proliferation of HL-60 cells was obviously inhibited and the differentiation was induced by both ATRA and HMBA. The decreased expression of CD44 and cyclin E mRNA, and the increased expression of p27 and p21 at mRNA levels were observed. Furthermore, there was a negative correlation between the expression of CD44 and p27. It was concluded that ATRA and HMBA played a role in the differentiation induction of HL-60 cells, which was mediated by the down-regulation of CD44, accompanied by down-regulation of cyclin E, and up-regulation of p27 and p21 mRNA. Cellular ＆ Molecular Immunology. 2007;4（1）：59-63.展开更多
文摘Telomerase activity was found to be high in various human cancers, but absent in most normal tissues. Its expression pattern made it a novel target for antitumor agents. Several strategies against telomerase were presented in this review. Targeting the telomerase RNA component by oligonucleotide/ribozyme was considered to be one of the most hopeful approaches. Some progresses were made in this area, such as the use of PANs and 2–5A antisense compounds. The relationships among telomerase activity and cell differentiation, signal transduction, oncogene, tumor suppressor gene as well as cell cycle modulation also provided a series of valuable ideas in designing anti-telomerase drugs for cancer therapy. In conclusion, although there is still a long way in understanding the mechanism and regulation of telomerase, the advance of studies on telomerase has allowed the development of numerous strategies for the treatment of cancer.
文摘Objective： To investigate the mechanism of moxibustion in regulating cellular apoptosis in rat＇s precancerous lesion of primary hepatocellular carcinoma （HCC）. Methods： Seventy-four rats were randomly allocated to normal group, model group and moxibustion group, and the diethylic nitrosamine （DEN） was used to establish HCC model. Moxibustion with moxa cone which is as big as a grain of wheat was performed on acupoint Zusanli （ST 36）, 3 cones for each acupoint and 0.5 mg for each cone, the treatment was given once a day, totally 16 weeks. Then the changes in the body weight, liver weight and thymus weight, a morphological change in the liver tissue and changes in γ-GT and GST were observed; Immunohistochemical staining method was adopted to observe the tendency of changes in relevant apoptosis genes such as C-myc, N-ras and mutant type P53, and the influence of moxibustion on cell cycle modulation genes such as cyclinD1, CDK4 and pl6. Results： Moxibustion could reduce the activities of γ-GT and GST in the blood, obviously decrease the protein expression of relevant apoptosis genes such as C-myc, N-ras and mutant type P53 and markedly inhibit the over-expression of relevant cell cycle modulation genes such as cyclinD1 and CDK4 and the mutation of cell cycle modulation gene pl 6. Conclusion： Moxibustion might play a certain role in relieving HCC precancerous lesion and its action mechanism might be related to the regulation on partial apoptosis genes.
文摘CD44 is highly expressed in human acute myeloid leukemia （AML） cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid （ATRA） and hexamethylene bisacetamide （HMBA）, but so far the mechanism was not demonstrated clearly. In the present study, we investigated whether ATRA or HMBA induced the growth arrest of HL-60 cells by down-regulating the expression of CD44. The results indicated that the proliferation of HL-60 cells was obviously inhibited and the differentiation was induced by both ATRA and HMBA. The decreased expression of CD44 and cyclin E mRNA, and the increased expression of p27 and p21 at mRNA levels were observed. Furthermore, there was a negative correlation between the expression of CD44 and p27. It was concluded that ATRA and HMBA played a role in the differentiation induction of HL-60 cells, which was mediated by the down-regulation of CD44, accompanied by down-regulation of cyclin E, and up-regulation of p27 and p21 mRNA. Cellular ＆ Molecular Immunology. 2007;4（1）：59-63.
