In vivo imaging system (IVIS) is a new and rapidly expanding technology, which has a wide range of applications in life science such as cell tracing. By counting the number of photons emitted from a specimen, IVIS can...In vivo imaging system (IVIS) is a new and rapidly expanding technology, which has a wide range of applications in life science such as cell tracing. By counting the number of photons emitted from a specimen, IVIS can quantify biological events such as tumor growth. We used B16F10-luc-G5 tumor cells and 20 Babl/C mice injected subcutaneously with B16F10-luc-G5 tumor cells (1×106 in 100 μL) to develop a method to quantitatively analyze cells traced by IVIS in vitro and in vivo, respectively. The results showed a strong correlation between the number of tumor cells and the intensity of bioluminescence signal (R2=0.99) under different exposure conditions in in vitro assay. The results derived from the in vivo experiments showed that tumor luminescence was observed in all mice by IVIS at all days, and there was significant difference (P<0.01) between every two days from day 3 to day 14. Moreover, tumor dynamic morphology could be monitored by IVIS when it was in- visible. There was a strong correlation between tumor volume and bioluminescence signal (R2=0.97) by IVIS. In summary, we demonstrated a way to accurately carry out the quantitative analysis of cells using IVIS both in vitro and in vivo. The data indicate that IVIS can be used as an effective and quantitative method for cell tracing both in vitro and in vivo.展开更多
Neurodegenerative disease is a brain disorder caused by the loss of structure andfunction of neurons that lowers the quality of human life. Apart from the limitedpotential for endogenous regeneration, stem cell-based ...Neurodegenerative disease is a brain disorder caused by the loss of structure andfunction of neurons that lowers the quality of human life. Apart from the limitedpotential for endogenous regeneration, stem cell-based therapies hold considerablepromise for maintaining homeostatic tissue regeneration and enhancingplasticity. Despite many studies, there remains insufficient evidence for stem celltracing and its correlation with endogenous neural cells in brain tissue with threedimensionalstructures. Recent advancements in tissue optical clearing techniqueshave been developed to overcome the existing shortcomings of cross-sectionaltissue analysis in thick and complex tissues. This review focuses on recentprogress of stem cell treatments to improve neurodegenerative disease, andintroduces tissue optical clearing techniques that can implement a threedimensionalimage as a proof of concept. This review provides a more comprehensiveunderstanding of stem cell tracing that will play an important role inevaluating therapeutic efficacy and cellular interrelationship for regeneration inneurodegenerative diseases.展开更多
ObjectiFe To evaluate the changes of pancreatic acinar cell functions in the rats with acutenecrotizing pancreatitis (ANP). methods Seventy SD rats were randomized into two groups: experimental group(n=35) and control...ObjectiFe To evaluate the changes of pancreatic acinar cell functions in the rats with acutenecrotizing pancreatitis (ANP). methods Seventy SD rats were randomized into two groups: experimental group(n=35) and control group (n=35). To prepare the experimental model, the retrograde injection of 5% sodiumtaurocholate into the pancreatic duct was used for inducing ANP. Radioactive tracing by L -3H-phenylalanineand autoradiography were performed for scoring the differences of changes of amino acid uptake, enzyme-proteinsynthesis and output from acinar cells in rats between both groups. Results No changes were observed in aminoacid uptake and enzyme -protein synthesis in rats with dotted and haemorrhagic necrotizing foci as compared withcontrol group. However, accumulated zymogen granules in the interstitial of acinar cells were seen in theexperimental group. Conclusion It indicates that in experimental ANP rats, the functions of acinar cells in bothamino acid uptake and protein synthesis were essentially normal, but the pathway of enzyme output was affectedinto ectopic secretion through the bottom or lateral cellular membrane of pancreatic acinar cell.展开更多
Studying embryonic hematopoiesis is complicated by diversity of its locations in the constantly changing anatomy and by the mobility of blood cell precursors.Embryonic hematopoietic progenitors are identified in tradi...Studying embryonic hematopoiesis is complicated by diversity of its locations in the constantly changing anatomy and by the mobility of blood cell precursors.Embryonic hematopoietic progenitors are identified in traditional in vivo and in vitro cell potential assays.Profound epigenetic plasticity of mammalian embryonic cells combined with significant inductive capacity of the potential assays suggest that our understanding of hematopoietic ontogenesis is substantially distorted.Non-invasive in vivo cell tracing methodology offers a better insight into complex processes of blood cell specification.In contrast to the widely accepted view based on the cell potential assays,the genetic tracing approach identified the yolk sac as the source of adult hematopoietic stem cell lineage.Realistic knowledge of the blood origin is critical for safe and efficient recapitulation of hematopoietic development in culture.展开更多
基金supported by a grant from the National Natural Sciences Foundation of China (No. 30901364)grants from the National Basic Research Program of China (No. 2003CB515505, 2009CB522407)
文摘In vivo imaging system (IVIS) is a new and rapidly expanding technology, which has a wide range of applications in life science such as cell tracing. By counting the number of photons emitted from a specimen, IVIS can quantify biological events such as tumor growth. We used B16F10-luc-G5 tumor cells and 20 Babl/C mice injected subcutaneously with B16F10-luc-G5 tumor cells (1×106 in 100 μL) to develop a method to quantitatively analyze cells traced by IVIS in vitro and in vivo, respectively. The results showed a strong correlation between the number of tumor cells and the intensity of bioluminescence signal (R2=0.99) under different exposure conditions in in vitro assay. The results derived from the in vivo experiments showed that tumor luminescence was observed in all mice by IVIS at all days, and there was significant difference (P<0.01) between every two days from day 3 to day 14. Moreover, tumor dynamic morphology could be monitored by IVIS when it was in- visible. There was a strong correlation between tumor volume and bioluminescence signal (R2=0.97) by IVIS. In summary, we demonstrated a way to accurately carry out the quantitative analysis of cells using IVIS both in vitro and in vivo. The data indicate that IVIS can be used as an effective and quantitative method for cell tracing both in vitro and in vivo.
基金the National Research Foundation of Korea(NRF)Grant funded by the Korea government(MSIT),No.NRF-2020R1C1C1013535.
文摘Neurodegenerative disease is a brain disorder caused by the loss of structure andfunction of neurons that lowers the quality of human life. Apart from the limitedpotential for endogenous regeneration, stem cell-based therapies hold considerablepromise for maintaining homeostatic tissue regeneration and enhancingplasticity. Despite many studies, there remains insufficient evidence for stem celltracing and its correlation with endogenous neural cells in brain tissue with threedimensionalstructures. Recent advancements in tissue optical clearing techniqueshave been developed to overcome the existing shortcomings of cross-sectionaltissue analysis in thick and complex tissues. This review focuses on recentprogress of stem cell treatments to improve neurodegenerative disease, andintroduces tissue optical clearing techniques that can implement a threedimensionalimage as a proof of concept. This review provides a more comprehensiveunderstanding of stem cell tracing that will play an important role inevaluating therapeutic efficacy and cellular interrelationship for regeneration inneurodegenerative diseases.
文摘ObjectiFe To evaluate the changes of pancreatic acinar cell functions in the rats with acutenecrotizing pancreatitis (ANP). methods Seventy SD rats were randomized into two groups: experimental group(n=35) and control group (n=35). To prepare the experimental model, the retrograde injection of 5% sodiumtaurocholate into the pancreatic duct was used for inducing ANP. Radioactive tracing by L -3H-phenylalanineand autoradiography were performed for scoring the differences of changes of amino acid uptake, enzyme-proteinsynthesis and output from acinar cells in rats between both groups. Results No changes were observed in aminoacid uptake and enzyme -protein synthesis in rats with dotted and haemorrhagic necrotizing foci as compared withcontrol group. However, accumulated zymogen granules in the interstitial of acinar cells were seen in theexperimental group. Conclusion It indicates that in experimental ANP rats, the functions of acinar cells in bothamino acid uptake and protein synthesis were essentially normal, but the pathway of enzyme output was affectedinto ectopic secretion through the bottom or lateral cellular membrane of pancreatic acinar cell.
文摘Studying embryonic hematopoiesis is complicated by diversity of its locations in the constantly changing anatomy and by the mobility of blood cell precursors.Embryonic hematopoietic progenitors are identified in traditional in vivo and in vitro cell potential assays.Profound epigenetic plasticity of mammalian embryonic cells combined with significant inductive capacity of the potential assays suggest that our understanding of hematopoietic ontogenesis is substantially distorted.Non-invasive in vivo cell tracing methodology offers a better insight into complex processes of blood cell specification.In contrast to the widely accepted view based on the cell potential assays,the genetic tracing approach identified the yolk sac as the source of adult hematopoietic stem cell lineage.Realistic knowledge of the blood origin is critical for safe and efficient recapitulation of hematopoietic development in culture.
