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Preliminary Validation of Tumor Cell Attachment Inhibition Assay for Developmental Toxicants With Mouse S180 Cells 被引量:3
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作者 LU RONG-ZHU CHEN CHUAN-FEN +1 位作者 LIN HUI-FEN HUANG LEI-MING AND JIN XI-PENG.(Department of Preventive Medicine, Zhenjiang Medical College, 3 YizhengRoad, Zhedeng, 212001 China)(Department of Occupational Health,School of Public Health, Shanghai Medical Univer 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1999年第4期253-259,共7页
This study was designed to explore the possibility of using ascitic mouse sarcoma cell line (S180) to validate the mouse tumor cell attachment assay for developmental toxicants, and to test the inhibitory effects of v... This study was designed to explore the possibility of using ascitic mouse sarcoma cell line (S180) to validate the mouse tumor cell attachment assay for developmental toxicants, and to test the inhibitory effects of various developmental toxicants. The results showed that 2 of 3 developmental toxicants under consideration, sodium pentobarbital and ethanol, significantly inhibited S180cells attachment to Concanavalin A-coaed surfaces. Inhibition was dependent on concentration, and the IC50 (the concentration tha reduced attachment by 50% ), of these 2 chemicals was 1.2×10-3mol/L and 1 .0 mol/L, respectively. Anoher developmental toxiant, hydmiortisone, did not show inhibitory activity. Two non-developmental toxicants, sodium chloride and glycine were also tested and these did not decrease attachment rates. The main results reported here were generally sindlar to those obtained with ascitic mouse ovdrian tumor cells as a model. Therefore, this study added further evidence to the conclusion that cell specificity does not lindt attachment inhibition to Con A-coated surfaces, so S180 cell may serve as an altemative cell model, especially when other cell lines are unavailable. Furthermore, after optimal validation, it can be suggested that an S180 cell attachment assay may be a candidate for a series of assays to detect developmental toxicants. 展开更多
关键词 cell cell In Preliminary validation of Tumor cell Attachment Inhibition Assay for Developmental Toxicants With Mouse S180 cells line
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