Current status and challenges for cell-cultured milk technology: a systematic review

Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular agriculture.This review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk production.Cellular agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular agriculture.Currently,various companies synthesize milk components through precision fermentation technology.Nevertheless,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation technology.Hence,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia.

HPD及合成卟啉化合物对HEP－2细胞的光敏作用比较

ＨＥｐ－２细胞对ＨＰＤ及九种卟啉化合物中的７种具荧光卟啉均能吸收。脂溶性的四种卟啉化合物易被含１０％小牛血清的Ｈａｎｋ＇ｓ液洗脱，总ＨＰＤ及３种水溶性卟啉则不易被洗脱出细胞。含有ＨＰＤ及以上７种卟啉化合物的细胞加光照时均可杀伤细胞。光敏活性ＨＰＤ＞脂溶性卟啉＞水溶性卟啉．另外两种含顺磁盒属的脂溶性卟啉化合物本身不具荧光，加到培养基中使细胞吸收，加光照后亦无光敏现象。实验说明卟啉的极性在光敏反应中起重要作用。

利用CO_2培养箱敞开式不换液羊水细胞培养方法的改进

本文对羊水培养方法进行了改进研究,探讨了培养液 pH 值对羊水细胞生长的影响。结果表明用 CO_2培养箱敞开培养,在 CO_2浓度为5.4%时羊水细胞最容易贴壁生长,一次性加液中间不换液可保持细胞生长的微环境稳定,是羊水细胞培养成功的关键。

左旋四氢巴马汀对单个豚鼠心室肌细胞钙电流的阻滞作用(英文)

目的:研究左旋四氢巴马汀(l-THP)对L-钙电流的作用。方法:用全细胞膜片箝记录心室肌细胞的L-钙电流。结果:l-THP 1-100μmol·L^(-1)将I_(Ca^(2+)-max)从(999±93)PA分别减少到(700±111)pA(582±66)pA和(420±112)pA(n=6,P<0.01)。l-THP对钙通道有紧张性阻滞作用及使用依赖性。l-THP可将钙通道的恢复时间从(94±39)ms延长到(170±42)ms(n=6,P<0.01)。结论:l-THP对L-钙通道有阻滞作用。