Distinctively directing endothelial cells(ECs)and smooth muscle cells(SMCs),potentially by surface topography cue,is of central importance for enhancing bioefficacy of vascular implants.For the first time,surface grad...Distinctively directing endothelial cells(ECs)and smooth muscle cells(SMCs),potentially by surface topography cue,is of central importance for enhancing bioefficacy of vascular implants.For the first time,surface gradients with a broad range of nano-micrometer roughness are developed on Mg,a promising next-generation biodegradable metal,to carry out a systematic study on the response of ECs and SMCs.Cell adhesion,spreading,and proliferation are quantified along gradients by high-throughput imaging,illustrating drastic divergence between ECs and SMCs,especially in highly rough regions.The profound role of surface topography overcoming the biochemical cue of released Mg2+is unraveled at different roughness ranges for ECs and SMCs.Further insights into the underlying regulatory mechanism are gained at subcellular and gene levels.Our work enables highefficient exploration of optimized surface morphology for modulating favored cell selectivity of promoting ECs and suppressing SMCs,providing a potential strategy to achieve rapid endothelialization for Mg.展开更多
基金the National Key Research and Development Program of China[grant numbers 2016YFC1102401,2016YFB0301001]National Natural Science Foundation of China[grant number 51701041]+1 种基金the Committee of Shanghai Science and Technology[grant number 17DZ2200200]Shanghai Outstanding Academic Leaders Plan[grant number 17XD1402100]。
文摘Distinctively directing endothelial cells(ECs)and smooth muscle cells(SMCs),potentially by surface topography cue,is of central importance for enhancing bioefficacy of vascular implants.For the first time,surface gradients with a broad range of nano-micrometer roughness are developed on Mg,a promising next-generation biodegradable metal,to carry out a systematic study on the response of ECs and SMCs.Cell adhesion,spreading,and proliferation are quantified along gradients by high-throughput imaging,illustrating drastic divergence between ECs and SMCs,especially in highly rough regions.The profound role of surface topography overcoming the biochemical cue of released Mg2+is unraveled at different roughness ranges for ECs and SMCs.Further insights into the underlying regulatory mechanism are gained at subcellular and gene levels.Our work enables highefficient exploration of optimized surface morphology for modulating favored cell selectivity of promoting ECs and suppressing SMCs,providing a potential strategy to achieve rapid endothelialization for Mg.
