Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a p...Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.展开更多
Cell mechanics is essential to cell development and function,and its dynamics evolution reflects the physiological state of cells.Here,we investigate the dynamical mechanical properties of single cells under various d...Cell mechanics is essential to cell development and function,and its dynamics evolution reflects the physiological state of cells.Here,we investigate the dynamical mechanical properties of single cells under various drug conditions,and present two mathematical approaches to quantitatively characterizing the cell physiological state.It is demonstrated that the cellular mechanical properties upon the drug action increase over time and tend to saturate,and can be mathematically characterized by a linear timeinvariant dynamical model.It is shown that the transition matrices of dynamical cell systems significantly improve the classification accuracies of the cells under different drug actions.Furthermore,it is revealed that there exists a positive linear correlation between the cytoskeleton density and the cellular mechanical properties,and the physiological state of a cell in terms of its cytoskeleton density can be predicted from its mechanical properties by a linear regression model.This study builds a relationship between the cellular mechanical properties and the cellular physiological state,adding information for evaluating drug efficacy.展开更多
Collective cell groups are organized to form specific patterns that play an important role in various physiological and pathological processes,such as tissue morphogenesis,wound healing,and cancer invasion.Compared to...Collective cell groups are organized to form specific patterns that play an important role in various physiological and pathological processes,such as tissue morphogenesis,wound healing,and cancer invasion.Compared to the behaviors of single cells that have been studied intensively from many aspects(cell migration,adhesion,polarization,proliferation,etc.)and at various length scales(molecular,subcellular,and cellular),the behaviors of multiple cells are less well understood,particularly from a quantitative perspective.In this talk,we present our recent studies of collective polarization and orientation of multiple cells through both experimental measurement and theoretical modeling,including cell behavior on/in 2D and 3D substrate/tissue.We find that collective cell behavior,including polarization,alignment,and migration,is closely related to local stress states in cell layers or tissue,which demonstrates the crucial role of mechanical forces in living organisms.Specifically,cells demonstrate preferential polarization and alignment along the maximum principal stress in the cell layer,and the cell aspect ratio increases with in-plane maximum shear stress,suggesting that the maximum shear stress is the underlying driving force of cell polarization and orientation.This theory of stress-driven cell behaviors of polarization and orientation provides a new perspective for understanding cell behaviors in living organisms and a guideline for tissue engineering in potential biomedical applications.Strikingly,we note that with regard to the polarization and alignment of collective cells,a typical feature of cell morphology is that the cells generally align along the edge of the pattern,which was called edge effect or boundary effect by assuming that the edge plays a role in cell alignment due to a phenomenon of chemistry.However,the edge effect is an obscure explanation.Here we showed that the edge effect could be explained by the theory of stress-driven cell behavior,i.e.,inplane stress-driven cell polarization and alignment.That is,the cell layer has a stress-free boundary condition at the edge,and thus the direction of the maximum principal stress should be precisely along the edge.According to the theory of stress-driven cell polarity,the cells then preferentially align with the edge of the cell layer,independently of the geometry of the pattern.Once there is a force-free condition at the edge or the boundary,the cells align along the edge of the pattern.Otherwise,the cell may not align with the edge;for example,the cells preferentially align in the radial direction of the wound because of the presence of the contractile force by the actin ring at the wound edge,which is in contradiction with the so-called edge effect but consistent with our theory of stress-driven cell polarity.展开更多
Cross-talk between tumor cells and mechanical stress in the tumor microenvironment has been shown to be involved in carcinogenesis.High mechanical stress in tumors can alter the metabolism and behaviors of cancer cell...Cross-talk between tumor cells and mechanical stress in the tumor microenvironment has been shown to be involved in carcinogenesis.High mechanical stress in tumors can alter the metabolism and behaviors of cancer cells and cause cancer cells to attain cancer stem-like cell properties,thus driving tumor progression and promoting metastasis.The mechanical signal is converted into a biochemical signal that activates tumorigenic signaling pathways through mechanotransduction.Herein,we describe the physical changes occurring during reprogramming of cancer cell metabolism,which regulate cancer stem cell functions and promote tumor progression and aggression.Furthermore,we highlight emerging therapeutic strategies targeting mechanotransduction signaling pathways.展开更多
Mechanical stimulations have been shown to regulate cellular mechanical properties. However, the stimulation patterns for effective regulation are as yet unclear. We investigated the effects of application of differin...Mechanical stimulations have been shown to regulate cellular mechanical properties. However, the stimulation patterns for effective regulation are as yet unclear. We investigated the effects of application of differing numbers of mechanical stimulation sets, each set consisting of 8% extension and compression to cells via deformation of cell culture elastic chamber, on cellular elasticity. Elasticity increased with only a single step-like stretch and with a single step-like stretch after 1 set of mechanical stimulation, whereas elasticity did not change with a single step-like stretch after 10 sets of mechanical stimulation. These results indicate that the increase in cellular elasticity with the single step-like stretch depends on the number of applied mechanical stimulations. Immunofluorescence staining showed that phosphorylation and dephosphorylation of myosin regulatory light chain (MRLC), which regulates intracellular contractile force and cellular elasticity, accompanied cellular elasticity changes. These findings suggest that cellular elasticity changes under cyclic and step-like stretches are mediated by MRLC.展开更多
Objective:To explore the mechanism of Nauclea Officinalis of anti-liver cancer effect based on network pharmacology,and to preliminarily verify anti-liver cancer activity of Nauclea Officinalis through cell screening....Objective:To explore the mechanism of Nauclea Officinalis of anti-liver cancer effect based on network pharmacology,and to preliminarily verify anti-liver cancer activity of Nauclea Officinalis through cell screening.Methods:Network pharmacology was used to screen for common targets of Nauclea Officinalis and liver cancer,protein-protein interaction(PPI)network was constructed,and enrichment analysis and mechanism prediction were conductd.Molecular docking of main active ingredients of Nauclea Officinalis with core targets was made.Preliminary verification was performed by in vitro cell experiments such as CCK8,cell apoptosis,and PCR.Results:After the screening,14 active ingredients of Nauclea Officinalis were obtained,with 587 related targets.After mapping with liver cancer targets,there were 288 common targets,mainly including TP53,SRC,STAT3,and other core targets.Among them,compounds such as strictosamide,pumiloside and vincosamide may be potential active ingredients of Nauclea Officinalis of anti-liver cancer effect.They may participate in protein phosphorylation and negative regulation of the apoptosis process by mediating cancer pathways,PI3K/Akt and EGFR tyrosine kinase inhibitors resistance signaling pathways to play an anti-liver cancer role;molecular docking results showd that active ingredients of Nauclea Officinalis had a stable binding with liver cancer core targets;in vitro cell experiments showd that main ingredient strictosamide of Nauclea Officinalis had cytotoxicity against liver cancer cells,inhibited liver cancer cell proliferation(P<0.001),down-regulated gene expression of liver cancer HepG2 cells SRC,STAT3,MAPK3(P<0.05),and induced liver cancer cell apoptosis(P<0.001).Conclusion:This study preliminarily explores the potential mechanism of active ingredients of Nauclea Officinalis against liver cancer and its preliminary pharmacological effects,providing a theoretical basis for the study of Nauclea Officinalis of anti-liver cancer mechanism.展开更多
Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperat...Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy(AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.展开更多
Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties o...Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a microsphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis.展开更多
Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or em...Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or embedded in the substrate underneath the cell.The former measures rheological properties of the cell such as cell stiffness,and the latter measures cell traction force dynamics.Here we describe the principles of these two cell mechanics research tools and an example of using them to study physical behaviors of the living cell in response to transient stretch or compression.We demonstrate that,when subjected to a stretchunstretch manipulation,both the stiffness and traction force of adherent cells promptly reduced,and then gradually recover up to the level prior to the stretch.Immunofluorescent staining and Western blotting results indicate that the actin cytoskeleton of the cells underwent a corresponding disruption and reassembly process almost in step with the changes of cell mechanics.Interestingly,when subjected to compression,the cells did not show such particular behaviors.Taken together,we conclude that adherent cells are very sensitive to the transient stretch but not transient compression,and the stretch-induced cell response is due to the dynamics of actin polymerization.展开更多
In this editorial,we discuss the remarkable role of physical energies in the control of cell signaling networks and in the specification of the architectural plan of both somatic and stem cells.In particular,we focus ...In this editorial,we discuss the remarkable role of physical energies in the control of cell signaling networks and in the specification of the architectural plan of both somatic and stem cells.In particular,we focus on the biological relevance of bioelectricity in the pattern control that orchestrates both developmental and regenerative pathways.To this end,the narrative starts from the dawn of the first studies on animal electricity,reconsidering the pioneer work of Harold Saxton Burr in the light of the current achievements.We finally discuss the most recent evidence showing that bioelectric signaling is an essential component of the informational processes that control pattern specification during embryogenesis,regeneration,or even malignant transformation.We conclude that there is now mounting evidence for the existence of a Morphogenetic Code,and that deciphering this code may lead to unprecedented opportunities for the development of novel paradigms of cure in regenerative and precision medicine.展开更多
Estrogen deficiency has been proposed as a risk factor for alveolar bone loss, but whether or not estrogen will influcence the bone rebuilting process during orthodontic tooth movement and what the mechanisms involved...Estrogen deficiency has been proposed as a risk factor for alveolar bone loss, but whether or not estrogen will influcence the bone rebuilting process during orthodontic tooth movement and what the mechanisms involved remain unclear. The paper aims to provide new information that may elucidate the modulatory effect of estrogen on the bone-resorbing cytokines RANKL and its anti-resorptive factor OPG secrected by HPLFs which are already force-stimulated. The expression of OPG mRNA is rising after mechanical loading either with or without stimulated by estrogen before. But HPDL cells exposured to estrogen for 24 h before loaded tend to express more OPG mRNA. Compared with the no-estrogen group, the inhibit trend of RANKL mRNA is much more apparent in with-estrogen group. Moreover, estrogen and mechanic force time-dependently increased OPG expression and attenuated the RANKL expression.展开更多
目的探讨miR-193a-3p/E2F6在鼻咽癌(NPC)中对细胞焦亡的调控作用及其潜在的分子机制。方法双荧光素酶基因报告实验评估了miR-193a-3p对E2F6的靶向调控作用。HNE-2细胞分为不同处理组:对照组、空白质粒组、沉默E2F6质粒组、空白质粒(miR...目的探讨miR-193a-3p/E2F6在鼻咽癌(NPC)中对细胞焦亡的调控作用及其潜在的分子机制。方法双荧光素酶基因报告实验评估了miR-193a-3p对E2F6的靶向调控作用。HNE-2细胞分为不同处理组:对照组、空白质粒组、沉默E2F6质粒组、空白质粒(miRNA)组、过表达miR-193a-3p组、过表达miRNA+空白(E2F6)质粒组以及过表达miRNA+过表达(E2F6)质粒组。通过qPCR检测miR-193a-3p的表达水平,Western blot检测E2F6蛋白的表达以及NLRP3/Caspase-1/GSDMD/GSDMD-N信号通路的激活情况。同时,使用扫描电子显微镜(scanning electron microscope,SEM)观察细胞形态,CCK-8法评估细胞增殖情况,进行集落形成实验和Transwell实验来检测细胞集落形成数量和迁移数量。结果与对照组(0.90±0.02)和空白质粒组(0.88±0.02)相比,沉默E2F6质粒组中E2F6蛋白(0.26±0.02)的表达水平显著降低,NLRP3/Caspase-1/GSDMD/GSDMD-N信号通路明显被激活,SEM显示细胞发生肿胀。CCK-8实验、集落形成实验和Transwell实验显示细胞活力、集落形成数量和迁移数量均下降(P均<0.05)。双荧光素酶报告基因实验证实E2F6是miR-193a-3p的靶基因。同对照组(1.01±0.03,1.02±0.02)和空白质粒(miRNA)组(1.02±0.04,0.21±0.03)相比,过表达miR-193a-3p组(2.02±0.32,0.23±0.02)中的miR-193a-3p的表达水平升高,E2F6的表达水平显著降低。而NLRP3/Caspase-1/GSDMD/GSDMD-N信号通路明显被激活,SEM显示细胞发生肿胀。CCK-8实验、集落形成实验和Transwell实验显示细胞活力、集落形成数量和迁移数量均下降(P均<0.05)。同过表达miR-193a-3p组和过表达miRNA+空白(E2F6)质粒组相比,发现过表达miR-193a-3p结合过表达E2F6组中E2F6蛋白的含量上升,NLRP3/Caspase-1/GSDMD/GSDMD-N蛋白的含量下降,SEM显示细胞肿胀减轻,CCK-8实验、集落形成实验和Transwell实验显示细胞活力、集落形成数量和迁移数量均上升(P均<0.05)。结论miR-193a-3p通过靶向抑制E2F6激活NLRP3/Caspase-1/GSDMD/GSDMD-N通路,诱导NPC细胞发生细胞焦亡,并抑制其增殖、克隆和侵袭。展开更多
基金supported by the National Key Research and Development Program of China,Nos.2017YFE0122900(to BH),2019YFA0110800(to WL),2019YFA0903802(to YW),2021YFA1101604(to LW),2018YFA0108502(to LF),and 2020YFA0804003(to JW)the National Natural Science Foundation of China,Nos.31621004(to WL,BH)and 31970821(to YW)+1 种基金CAS Project for Young Scientists in Basic Research,No.YSBR-041(to YW)Joint Funds of the National Natural Science Foundation of China,No.U21A20396(to BH)。
文摘Numerous studies have shown that cell replacement therapy can replenish lost cells and rebuild neural circuitry in animal models of Parkinson’s disease.Transplantation of midbrain dopaminergic progenitor cells is a promising treatment for Parkinson’s disease.However,transplanted cells can be injured by mechanical damage during handling and by changes in the transplantation niche.Here,we developed a one-step biomanufacturing platform that uses small-aperture gelatin microcarriers to produce beads carrying midbrain dopaminergic progenitor cells.These beads allow midbrain dopaminergic progenitor cell differentiation and cryopreservation without digestion,effectively maintaining axonal integrity in vitro.Importantly,midbrain dopaminergic progenitor cell bead grafts showed increased survival and only mild immunoreactivity in vivo compared with suspended midbrain dopaminergic progenitor cell grafts.Overall,our findings show that these midbrain dopaminergic progenitor cell beads enhance the effectiveness of neuronal cell transplantation.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos:U1908215,61925307,62003338,and 61933008)CAS Project for Young Scientists in Basic Research(Grant No:YSBR-041)+2 种基金Liaoning Revitalization Talents Program(Grant No:XLYC2002014)Natural Science Foundation of Liaoning Province of China(Grant No:2020-ZLLH-47)Joint fund of Science&Technology Department of Liaoning Province and State Key Laboratory of Robotics,China(Grant No:2019-KF-01-01).
文摘Cell mechanics is essential to cell development and function,and its dynamics evolution reflects the physiological state of cells.Here,we investigate the dynamical mechanical properties of single cells under various drug conditions,and present two mathematical approaches to quantitatively characterizing the cell physiological state.It is demonstrated that the cellular mechanical properties upon the drug action increase over time and tend to saturate,and can be mathematically characterized by a linear timeinvariant dynamical model.It is shown that the transition matrices of dynamical cell systems significantly improve the classification accuracies of the cells under different drug actions.Furthermore,it is revealed that there exists a positive linear correlation between the cytoskeleton density and the cellular mechanical properties,and the physiological state of a cell in terms of its cytoskeleton density can be predicted from its mechanical properties by a linear regression model.This study builds a relationship between the cellular mechanical properties and the cellular physiological state,adding information for evaluating drug efficacy.
基金supported by the National Natural Science Foundation of China ( 11772055,11532009)
文摘Collective cell groups are organized to form specific patterns that play an important role in various physiological and pathological processes,such as tissue morphogenesis,wound healing,and cancer invasion.Compared to the behaviors of single cells that have been studied intensively from many aspects(cell migration,adhesion,polarization,proliferation,etc.)and at various length scales(molecular,subcellular,and cellular),the behaviors of multiple cells are less well understood,particularly from a quantitative perspective.In this talk,we present our recent studies of collective polarization and orientation of multiple cells through both experimental measurement and theoretical modeling,including cell behavior on/in 2D and 3D substrate/tissue.We find that collective cell behavior,including polarization,alignment,and migration,is closely related to local stress states in cell layers or tissue,which demonstrates the crucial role of mechanical forces in living organisms.Specifically,cells demonstrate preferential polarization and alignment along the maximum principal stress in the cell layer,and the cell aspect ratio increases with in-plane maximum shear stress,suggesting that the maximum shear stress is the underlying driving force of cell polarization and orientation.This theory of stress-driven cell behaviors of polarization and orientation provides a new perspective for understanding cell behaviors in living organisms and a guideline for tissue engineering in potential biomedical applications.Strikingly,we note that with regard to the polarization and alignment of collective cells,a typical feature of cell morphology is that the cells generally align along the edge of the pattern,which was called edge effect or boundary effect by assuming that the edge plays a role in cell alignment due to a phenomenon of chemistry.However,the edge effect is an obscure explanation.Here we showed that the edge effect could be explained by the theory of stress-driven cell behavior,i.e.,inplane stress-driven cell polarization and alignment.That is,the cell layer has a stress-free boundary condition at the edge,and thus the direction of the maximum principal stress should be precisely along the edge.According to the theory of stress-driven cell polarity,the cells then preferentially align with the edge of the cell layer,independently of the geometry of the pattern.Once there is a force-free condition at the edge or the boundary,the cells align along the edge of the pattern.Otherwise,the cell may not align with the edge;for example,the cells preferentially align in the radial direction of the wound because of the presence of the contractile force by the actin ring at the wound edge,which is in contradiction with the so-called edge effect but consistent with our theory of stress-driven cell polarity.
基金the National Natural Science Foundation of China(Grant No.11832008 and 11772073)by the Program of the Postgraduate Tutor Team,Chongqing Education Commission(2018).
文摘Cross-talk between tumor cells and mechanical stress in the tumor microenvironment has been shown to be involved in carcinogenesis.High mechanical stress in tumors can alter the metabolism and behaviors of cancer cells and cause cancer cells to attain cancer stem-like cell properties,thus driving tumor progression and promoting metastasis.The mechanical signal is converted into a biochemical signal that activates tumorigenic signaling pathways through mechanotransduction.Herein,we describe the physical changes occurring during reprogramming of cancer cell metabolism,which regulate cancer stem cell functions and promote tumor progression and aggression.Furthermore,we highlight emerging therapeutic strategies targeting mechanotransduction signaling pathways.
文摘Mechanical stimulations have been shown to regulate cellular mechanical properties. However, the stimulation patterns for effective regulation are as yet unclear. We investigated the effects of application of differing numbers of mechanical stimulation sets, each set consisting of 8% extension and compression to cells via deformation of cell culture elastic chamber, on cellular elasticity. Elasticity increased with only a single step-like stretch and with a single step-like stretch after 1 set of mechanical stimulation, whereas elasticity did not change with a single step-like stretch after 10 sets of mechanical stimulation. These results indicate that the increase in cellular elasticity with the single step-like stretch depends on the number of applied mechanical stimulations. Immunofluorescence staining showed that phosphorylation and dephosphorylation of myosin regulatory light chain (MRLC), which regulates intracellular contractile force and cellular elasticity, accompanied cellular elasticity changes. These findings suggest that cellular elasticity changes under cyclic and step-like stretches are mediated by MRLC.
基金Major Science and Technology Project of Hainan Province(No.ZDKJ2019009)Construction Project of Clinical Medical Center in Hainan Province。
文摘Objective:To explore the mechanism of Nauclea Officinalis of anti-liver cancer effect based on network pharmacology,and to preliminarily verify anti-liver cancer activity of Nauclea Officinalis through cell screening.Methods:Network pharmacology was used to screen for common targets of Nauclea Officinalis and liver cancer,protein-protein interaction(PPI)network was constructed,and enrichment analysis and mechanism prediction were conductd.Molecular docking of main active ingredients of Nauclea Officinalis with core targets was made.Preliminary verification was performed by in vitro cell experiments such as CCK8,cell apoptosis,and PCR.Results:After the screening,14 active ingredients of Nauclea Officinalis were obtained,with 587 related targets.After mapping with liver cancer targets,there were 288 common targets,mainly including TP53,SRC,STAT3,and other core targets.Among them,compounds such as strictosamide,pumiloside and vincosamide may be potential active ingredients of Nauclea Officinalis of anti-liver cancer effect.They may participate in protein phosphorylation and negative regulation of the apoptosis process by mediating cancer pathways,PI3K/Akt and EGFR tyrosine kinase inhibitors resistance signaling pathways to play an anti-liver cancer role;molecular docking results showd that active ingredients of Nauclea Officinalis had a stable binding with liver cancer core targets;in vitro cell experiments showd that main ingredient strictosamide of Nauclea Officinalis had cytotoxicity against liver cancer cells,inhibited liver cancer cell proliferation(P<0.001),down-regulated gene expression of liver cancer HepG2 cells SRC,STAT3,MAPK3(P<0.05),and induced liver cancer cell apoptosis(P<0.001).Conclusion:This study preliminarily explores the potential mechanism of active ingredients of Nauclea Officinalis against liver cancer and its preliminary pharmacological effects,providing a theoretical basis for the study of Nauclea Officinalis of anti-liver cancer mechanism.
基金supported by the National Natural Science Foundation of China(61175103,61375107,61327014,61433017)the Research Fund of the State Key Laboratory of Robotics(2014-Z07)CAS FEA International Partnership Program for Creative Research Teams
文摘Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy(AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.
基金supported by the National Natural Science Foundation of China (Grant Nos. 60904095 and 61175103)CAS FEA International Partnership Program for Creative Research Teams and the State Key Laboratory of Drug Research
文摘Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a microsphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis.
文摘Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or embedded in the substrate underneath the cell.The former measures rheological properties of the cell such as cell stiffness,and the latter measures cell traction force dynamics.Here we describe the principles of these two cell mechanics research tools and an example of using them to study physical behaviors of the living cell in response to transient stretch or compression.We demonstrate that,when subjected to a stretchunstretch manipulation,both the stiffness and traction force of adherent cells promptly reduced,and then gradually recover up to the level prior to the stretch.Immunofluorescent staining and Western blotting results indicate that the actin cytoskeleton of the cells underwent a corresponding disruption and reassembly process almost in step with the changes of cell mechanics.Interestingly,when subjected to compression,the cells did not show such particular behaviors.Taken together,we conclude that adherent cells are very sensitive to the transient stretch but not transient compression,and the stretch-induced cell response is due to the dynamics of actin polymerization.
文摘In this editorial,we discuss the remarkable role of physical energies in the control of cell signaling networks and in the specification of the architectural plan of both somatic and stem cells.In particular,we focus on the biological relevance of bioelectricity in the pattern control that orchestrates both developmental and regenerative pathways.To this end,the narrative starts from the dawn of the first studies on animal electricity,reconsidering the pioneer work of Harold Saxton Burr in the light of the current achievements.We finally discuss the most recent evidence showing that bioelectric signaling is an essential component of the informational processes that control pattern specification during embryogenesis,regeneration,or even malignant transformation.We conclude that there is now mounting evidence for the existence of a Morphogenetic Code,and that deciphering this code may lead to unprecedented opportunities for the development of novel paradigms of cure in regenerative and precision medicine.
文摘Estrogen deficiency has been proposed as a risk factor for alveolar bone loss, but whether or not estrogen will influcence the bone rebuilting process during orthodontic tooth movement and what the mechanisms involved remain unclear. The paper aims to provide new information that may elucidate the modulatory effect of estrogen on the bone-resorbing cytokines RANKL and its anti-resorptive factor OPG secrected by HPLFs which are already force-stimulated. The expression of OPG mRNA is rising after mechanical loading either with or without stimulated by estrogen before. But HPDL cells exposured to estrogen for 24 h before loaded tend to express more OPG mRNA. Compared with the no-estrogen group, the inhibit trend of RANKL mRNA is much more apparent in with-estrogen group. Moreover, estrogen and mechanic force time-dependently increased OPG expression and attenuated the RANKL expression.
文摘目的探讨miR-193a-3p/E2F6在鼻咽癌(NPC)中对细胞焦亡的调控作用及其潜在的分子机制。方法双荧光素酶基因报告实验评估了miR-193a-3p对E2F6的靶向调控作用。HNE-2细胞分为不同处理组:对照组、空白质粒组、沉默E2F6质粒组、空白质粒(miRNA)组、过表达miR-193a-3p组、过表达miRNA+空白(E2F6)质粒组以及过表达miRNA+过表达(E2F6)质粒组。通过qPCR检测miR-193a-3p的表达水平,Western blot检测E2F6蛋白的表达以及NLRP3/Caspase-1/GSDMD/GSDMD-N信号通路的激活情况。同时,使用扫描电子显微镜(scanning electron microscope,SEM)观察细胞形态,CCK-8法评估细胞增殖情况,进行集落形成实验和Transwell实验来检测细胞集落形成数量和迁移数量。结果与对照组(0.90±0.02)和空白质粒组(0.88±0.02)相比,沉默E2F6质粒组中E2F6蛋白(0.26±0.02)的表达水平显著降低,NLRP3/Caspase-1/GSDMD/GSDMD-N信号通路明显被激活,SEM显示细胞发生肿胀。CCK-8实验、集落形成实验和Transwell实验显示细胞活力、集落形成数量和迁移数量均下降(P均<0.05)。双荧光素酶报告基因实验证实E2F6是miR-193a-3p的靶基因。同对照组(1.01±0.03,1.02±0.02)和空白质粒(miRNA)组(1.02±0.04,0.21±0.03)相比,过表达miR-193a-3p组(2.02±0.32,0.23±0.02)中的miR-193a-3p的表达水平升高,E2F6的表达水平显著降低。而NLRP3/Caspase-1/GSDMD/GSDMD-N信号通路明显被激活,SEM显示细胞发生肿胀。CCK-8实验、集落形成实验和Transwell实验显示细胞活力、集落形成数量和迁移数量均下降(P均<0.05)。同过表达miR-193a-3p组和过表达miRNA+空白(E2F6)质粒组相比,发现过表达miR-193a-3p结合过表达E2F6组中E2F6蛋白的含量上升,NLRP3/Caspase-1/GSDMD/GSDMD-N蛋白的含量下降,SEM显示细胞肿胀减轻,CCK-8实验、集落形成实验和Transwell实验显示细胞活力、集落形成数量和迁移数量均上升(P均<0.05)。结论miR-193a-3p通过靶向抑制E2F6激活NLRP3/Caspase-1/GSDMD/GSDMD-N通路,诱导NPC细胞发生细胞焦亡,并抑制其增殖、克隆和侵袭。