Objective： LRIG1 gene is a newly found human gene that displays homologies to the Drosophila Kek-1 gene. Previous researches have shown that the LRIG1 gene almost expressed in all human tissues. However, its function...Objective： LRIG1 gene is a newly found human gene that displays homologies to the Drosophila Kek-1 gene. Previous researches have shown that the LRIG1 gene almost expressed in all human tissues. However, its functions, particularly its functions in human tumors, are still unknown. The goals of the present study are to magnify the expression spectrum of the LRIG1 gene and determine their roles in the oncogenesis. Methods： A triphasic oligonucleotide probe was designed and used to detect the expression level of the LRIG1 gene in 16 astrocytomas and the corresponding tissues around the tumors by in situ hybridization. 11 primary astrocytoma cells were cultured. Among these, the expression level of the LRIG1 gene was checked by in situ hybridization and the expression of the Proliferating Cell Nuclear Antigen （PCNA） protein was detected by immunohistochemistry. Results： The expression of LRIG1 protein was detected in different degree in all the tumors and the surrounding tissues. Compared to the surrounding tissues, the expression of the tumors was lower. The decrease extends from the surrounding tissues to the tumors were correlation to the tumors＇ grades. The primary cultured cells also expressed LRIG1 to various extent and the expression of LRIG1 in the cultures was negatively correlated with the intensity of the PCNA staining. Conclusion： The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor.展开更多
Objective： To investigate the effect of Jinmaitong （筋脉通,JMT） serum on the proliferation of rat Schwann cells （SCs） primarily cultured in high glucose medium. Method： SOs were primarily cultured in Dulbecco＇s...Objective： To investigate the effect of Jinmaitong （筋脉通,JMT） serum on the proliferation of rat Schwann cells （SCs） primarily cultured in high glucose medium. Method： SOs were primarily cultured in Dulbecco＇s minmum essential medium （DMEM control）, 50 mmol/L glucose medium （50 mmol/L Glu）, 75 mmol/L glucose medium （75 mmol/L Glu）, as well as 50 mmol/L glucose medium, with different concentrations of JMT serum （undiluted, 1：2 diluted and 1：8 diluted） and Neurotropin （Ntp）, respectively. The proliferation of SCs under different conditions was detected by MTT. Result： SCs grew exuberantly in DMEM within 24-72 h, but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48, 72 and 96 h, which showed that both were significantly different compared to the control group （P〈0.01）. The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu （P〈0.05）. Spearman＇s rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose （r=-0.471, P〈0.01）. Excluding the time factor, partial correlation showed similar results （r =-0.679, P〈0.01）. After 48 h, the proliferation of SCs increased significantly in JMT 1：2 and Ntp compared with 50 mmol/L Glu （control 0.437±0.019, 50 mmol/ L Glu 0.367±0.035, JMT1：2 0.426±0.024, Ntp 0.422±0.013; P〈0.01）, and there were no statistically significant differences among the JMT groups, the Ntp group and the control group （P〉0.05）. Conclusions： The proliferation of SCs was inhibited in high glucose medium, and the inhibition was reduced by different concentrations of JMT serum, especially at JMT1：2.展开更多
文摘Objective： LRIG1 gene is a newly found human gene that displays homologies to the Drosophila Kek-1 gene. Previous researches have shown that the LRIG1 gene almost expressed in all human tissues. However, its functions, particularly its functions in human tumors, are still unknown. The goals of the present study are to magnify the expression spectrum of the LRIG1 gene and determine their roles in the oncogenesis. Methods： A triphasic oligonucleotide probe was designed and used to detect the expression level of the LRIG1 gene in 16 astrocytomas and the corresponding tissues around the tumors by in situ hybridization. 11 primary astrocytoma cells were cultured. Among these, the expression level of the LRIG1 gene was checked by in situ hybridization and the expression of the Proliferating Cell Nuclear Antigen （PCNA） protein was detected by immunohistochemistry. Results： The expression of LRIG1 protein was detected in different degree in all the tumors and the surrounding tissues. Compared to the surrounding tissues, the expression of the tumors was lower. The decrease extends from the surrounding tissues to the tumors were correlation to the tumors＇ grades. The primary cultured cells also expressed LRIG1 to various extent and the expression of LRIG1 in the cultures was negatively correlated with the intensity of the PCNA staining. Conclusion： The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor.
基金Supported by the Natural Science Foundation of China(No.30572438)
文摘Objective： To investigate the effect of Jinmaitong （筋脉通,JMT） serum on the proliferation of rat Schwann cells （SCs） primarily cultured in high glucose medium. Method： SOs were primarily cultured in Dulbecco＇s minmum essential medium （DMEM control）, 50 mmol/L glucose medium （50 mmol/L Glu）, 75 mmol/L glucose medium （75 mmol/L Glu）, as well as 50 mmol/L glucose medium, with different concentrations of JMT serum （undiluted, 1：2 diluted and 1：8 diluted） and Neurotropin （Ntp）, respectively. The proliferation of SCs under different conditions was detected by MTT. Result： SCs grew exuberantly in DMEM within 24-72 h, but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48, 72 and 96 h, which showed that both were significantly different compared to the control group （P〈0.01）. The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu （P〈0.05）. Spearman＇s rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose （r=-0.471, P〈0.01）. Excluding the time factor, partial correlation showed similar results （r =-0.679, P〈0.01）. After 48 h, the proliferation of SCs increased significantly in JMT 1：2 and Ntp compared with 50 mmol/L Glu （control 0.437±0.019, 50 mmol/ L Glu 0.367±0.035, JMT1：2 0.426±0.024, Ntp 0.422±0.013; P〈0.01）, and there were no statistically significant differences among the JMT groups, the Ntp group and the control group （P〉0.05）. Conclusions： The proliferation of SCs was inhibited in high glucose medium, and the inhibition was reduced by different concentrations of JMT serum, especially at JMT1：2.
