Three bacterial strains Bacillus pumilis, Cellulosimicrobium cellulans and ExtguoOactertum were investigated when grown in Luria-Bertani （LB） medium at 500 μg/mL Cr（VI）. The hexavalent chromium reduction was meas...Three bacterial strains Bacillus pumilis, Cellulosimicrobium cellulans and ExtguoOactertum were investigated when grown in Luria-Bertani （LB） medium at 500 μg/mL Cr（VI）. The hexavalent chromium reduction was measured by growing the strains in DeLeo and Ehrlich （1994） medium at 200 and 400 μg/mL K2CrO4. The optimal Cr （VI） reduction by strains B. pumilis, Exigubacterium and C. cellulans was 51%, 39%, and 41%, respectively, at an initial KzCrO4 concentration of 200 ~tg/mL at pH 3 and temperature 37℃. At an initial chromate concentration of 400 gg/mL, the Cr（VI） reduction by strains B. purnilis, Exigubacterium and C. cellulans was 24%, 19%, and 18%, respectively at pH 3 at 37℃ after 24 h. These strains have ability to reduce toxic hexavalent chromium to the less mobile trivalent chromium at a wide range of different environmental conditions and can be useful for the treatment of contaminated wastewater and soils.展开更多
Tribenuron methyl （TBM） is a member of the sulfonylurea herbicide family and is widely used worldwide. In this study, TBM- degrading bacteria were enriched with TBM as potential carbon, nitrogen or sulfur source, an...Tribenuron methyl （TBM） is a member of the sulfonylurea herbicide family and is widely used worldwide. In this study, TBM- degrading bacteria were enriched with TBM as potential carbon, nitrogen or sulfur source, and 44 bacterial isolates were obtained. These isolates were phylogenetically diverse, and were grouped into 25 operational taxonomic units and 14 currently known genera. Three representatives, Bacillus sp. strain BS2, Microbacterium sp. strain BS3, and Cellulosimicrobium sp. strain BSI 1, were selected, and their growth and TBM removal from culture broth were investigated. In addition, indigenous earthworms were collected and applied to augment TBM degradation in lab-scale soil column experiments. Results demonstrated that Bacillus sp. strain BS2 and earthworms significantly increased TBM removal during soil column experiments.展开更多
文摘Three bacterial strains Bacillus pumilis, Cellulosimicrobium cellulans and ExtguoOactertum were investigated when grown in Luria-Bertani （LB） medium at 500 μg/mL Cr（VI）. The hexavalent chromium reduction was measured by growing the strains in DeLeo and Ehrlich （1994） medium at 200 and 400 μg/mL K2CrO4. The optimal Cr （VI） reduction by strains B. pumilis, Exigubacterium and C. cellulans was 51%, 39%, and 41%, respectively, at an initial KzCrO4 concentration of 200 ~tg/mL at pH 3 and temperature 37℃. At an initial chromate concentration of 400 gg/mL, the Cr（VI） reduction by strains B. purnilis, Exigubacterium and C. cellulans was 24%, 19%, and 18%, respectively at pH 3 at 37℃ after 24 h. These strains have ability to reduce toxic hexavalent chromium to the less mobile trivalent chromium at a wide range of different environmental conditions and can be useful for the treatment of contaminated wastewater and soils.
基金supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KSCX2-YW-G-052)
文摘Tribenuron methyl （TBM） is a member of the sulfonylurea herbicide family and is widely used worldwide. In this study, TBM- degrading bacteria were enriched with TBM as potential carbon, nitrogen or sulfur source, and 44 bacterial isolates were obtained. These isolates were phylogenetically diverse, and were grouped into 25 operational taxonomic units and 14 currently known genera. Three representatives, Bacillus sp. strain BS2, Microbacterium sp. strain BS3, and Cellulosimicrobium sp. strain BSI 1, were selected, and their growth and TBM removal from culture broth were investigated. In addition, indigenous earthworms were collected and applied to augment TBM degradation in lab-scale soil column experiments. Results demonstrated that Bacillus sp. strain BS2 and earthworms significantly increased TBM removal during soil column experiments.
