HIP-55(HPK1-interacting protein of 55 ku,又名DBNL,SH3P7,mAbp1)是一种与HPK-1相结合分子量为55 ku,由多个具有蛋白相互作用结构域构成的接头蛋白。其N端含有肌动蛋白结合结构域C端具有SH3结构域。HIP-55能够通过细胞内信号转导调控...HIP-55(HPK1-interacting protein of 55 ku,又名DBNL,SH3P7,mAbp1)是一种与HPK-1相结合分子量为55 ku,由多个具有蛋白相互作用结构域构成的接头蛋白。其N端含有肌动蛋白结合结构域C端具有SH3结构域。HIP-55能够通过细胞内信号转导调控细胞内吞、迁移、骨架重排等生物功能并且在多种疾病病理生理过程中发挥重要作用。该文将对HIP-55蛋白生物学功能及其胞内信号转导通路参与疾病病理过程分子机制进行综述。展开更多
Objective The purpose of this study was to investigate the anti-androgenic mechanism of cypermethrin involving coactivators.Methods Mammalian two-hybrid assays were performed to study the effects of cypermethrin on in...Objective The purpose of this study was to investigate the anti-androgenic mechanism of cypermethrin involving coactivators.Methods Mammalian two-hybrid assays were performed to study the effects of cypermethrin on interactions of the androgen receptor(AR)with the coactivators androgen receptor-associated protein70(ARA70)and androgen receptor-associated protein 55(ARA55).Results The results showed that AR–ARA70 and AR–ARA55 interactions were remarkably enhanced by dihydrotestosterone(DHT,P≤0.05).Cypermethrin inhibited DHT-induced AR–ARA70 and AR–ARA55 interactions significantly(P≤0.05).Conclusion The study indicates that cypermethrin exhibits inhibitory effects on AR transcription associated with repression of AR–ARA70 and AR–ARA55 interactions in a ligand-dependent manner.The data show novel anti-androgenic mechanisms of cypermethrin that contribute to male reproductive toxicology.展开更多
HIV-1 matrix protein (MA) is a multifunctional structural protein localized on N terminus of Gag precursor p55. MA participates in HIV-1 assembly as membranotropic part of Gag precursor as well as an individual protei...HIV-1 matrix protein (MA) is a multifunctional structural protein localized on N terminus of Gag precursor p55. MA participates in HIV-1 assembly as membranotropic part of Gag precursor as well as an individual protein spliced from Gag early in infection. MA is found in the nuclei of infected cells and in plasma membrane, the site of virus assembly, in association with viral genome RNA. MA mutated variant M4 which contains two changed amino acids in N-terminal regions is also associated with viral RNA, but it is localized in the nuclear and cytoskeleton fractions but not in the plasma membrane suggesting that the mutant is deprived of membranotropic signal and “sticks” in the nuclei an d cytoskeleton, its previous location sites. These data allow suggesting that MA involved into transmission of viral RNA is transported to plasma membrane by cytoskeleton.展开更多
目的探讨中心体相关蛋白55(CEP55)在结直肠癌组织中的表达及其与临床病理特征及预后的关系。方法选取2017年8月至2020年8月在唐山市中医医院确诊为结直肠癌并进行手术治疗的126例患者作为研究对象,收集结直肠癌患者术中切除的结直肠癌...目的探讨中心体相关蛋白55(CEP55)在结直肠癌组织中的表达及其与临床病理特征及预后的关系。方法选取2017年8月至2020年8月在唐山市中医医院确诊为结直肠癌并进行手术治疗的126例患者作为研究对象,收集结直肠癌患者术中切除的结直肠癌组织及癌旁组织(距离癌组织>2 cm)标本各126例。通过qPCR、免疫组化实验检测结直肠癌组织及癌旁组织中CEP55的表达,分析结直肠癌组织中CEP55的表达与临床病理特征的关系,采用Kaplan-Meier法绘制生存曲线,并分析结直肠癌组织中CEP55的表达与患者3年总生存率之间的关系。结果结直肠癌组织中CEP55 m RNA的表达高于癌旁组织【(1.0±0.2)vs.(0.6±0.1)】,差异有统计学意义(P<0.001)。免疫组化结果显示,CEP55在结直肠癌组织中的表达高于癌旁组织,结直肠癌组织中CEP55高表达比例高于癌旁组织(P<0.001)。结直肠癌组织中CEP55的表达与TNM分期有关(P<0.05),与性别、年龄、肿瘤分化程度、肿瘤最大直径、淋巴结转移、肿瘤位置、肿瘤浸润深度无关(P>0.05)。Ⅲ+Ⅳ期结直肠癌患者组织中CEP55的表达评分高于Ⅰ+Ⅱ期结直肠癌患者(P<0.001)。CEP55高表达的结直肠癌患者3年总生存率低于CEP55低表达的结直肠癌患者(70.3%vs.88.6%),差异有统计学意义(Log-rankχ^(2)=4.715,P=0.031)。结论CEP55在结直肠癌组织中高表达,与TNM分期有关,可能可以作为结直肠癌的潜在预后生物标志物。展开更多
The TicS5 （Translocon at the inner envelope membrane of chloroplasts, 55 kDa） protein was identified in pea as a putative regulator, possibly linking chloroplast protein import to the redox state of the photosynthet...The TicS5 （Translocon at the inner envelope membrane of chloroplasts, 55 kDa） protein was identified in pea as a putative regulator, possibly linking chloroplast protein import to the redox state of the photosynthetic machinery. Two Tic55 homologs have been proposed to exist in Arabidopsis： atTic55-11 and AtPTC52 （Protochlorophyllide-dependent Trans- Iocon Component, 52 kDa; has also been called atTic55-1V）. Our phylogenetic analysis shows that attic55-11 is an ortholog of psTic55 from pea （Pisum sativurn）, and that AtPTC52 is a more distant homolog of the two. AtPTC52 was included in this study to rule out possible functional links between the proteins in Arabidopsis. No detectable mutant phenotypes were found in two independent T-DNA knockout mutant plant lines for each Arabidopsis protein, when compared with wild- type： visible appearance, chlorophyll content, photosynthetic performance, and chloroplast protein import, for example, were all normal. Both wild-type and tic55-11 mutant chloroplasts exhibited deficient protein import when treated with diethylpyrocarbonate, indicating that Tic55 is not the sole target of this reagent in relation to protein import. Furthermore, ptc52 mutant chloroplasts were not defective with respect to pPORA import, which was previously reported to involve PTC52 in barley. Thus, we conclude that atTic55-11 and AtPTC52 are not strictly required for functional protein import in Arabidopsis.展开更多
目的探索中心体蛋白55(centrosome protein 55,CEP55)对膀胱癌细胞增殖能力的影响及相关分子机制。方法利用蛋白免疫印迹检测正常膀胱组织细胞(SV-HUC-1)与膀胱癌细胞(T24)CEP55、H3K9 me3的表达量。将膀胱癌细胞T24分为实验组和对照组...目的探索中心体蛋白55(centrosome protein 55,CEP55)对膀胱癌细胞增殖能力的影响及相关分子机制。方法利用蛋白免疫印迹检测正常膀胱组织细胞(SV-HUC-1)与膀胱癌细胞(T24)CEP55、H3K9 me3的表达量。将膀胱癌细胞T24分为实验组和对照组,实验组细胞转染siRNA-CEP55,对照组细胞转染siRNA-MOCK。利用蛋白免疫印迹实验检测各组细胞CEP55、H3K9、H3K9me3的表达量,利用CCK8实验检测各组细胞的增殖能力。结果蛋白免疫印迹实验结果表明,膀胱癌T24细胞CEP55表达量为0.83±0.15,H3K9me3表达量为1.01±0.19。膀胱上皮SV-HUC-1细胞CEP55表达量为0.35±0.09,H3K9me3表达量为0.44±0.10,膀胱癌细胞CEP55、H3K9me3的表达均高于正常膀胱细胞(均P<0.05)。siRNA-CEP55成功降低膀胱癌细胞T24 CEP55的表达。实验组T24细胞吸光度为1.109±0.105,明显低于对照组细胞的2.208±0.104,CEP55低表达会降低T24细胞的增殖能力(P<0.05)。蛋白免疫印迹结果显示实验组T24细胞H3K9变化不明显,H3K9me3表达(P<0.05)明显下降。结论CEP55能通过降低H3K9的三甲基化来抑制膀胱癌细胞的增殖能力。展开更多
Understanding the genetic basis of quality-related traits contributes to the improvement of grain protein concentration(GPC),grain starch concentration(GSC),and wet gluten concentration(WGC)in wheat.In this study,a ge...Understanding the genetic basis of quality-related traits contributes to the improvement of grain protein concentration(GPC),grain starch concentration(GSC),and wet gluten concentration(WGC)in wheat.In this study,a genome-wide association study(GWAS)based on a mixed linear model(MLM)was performed on 236 wheat accessions,including 160 cultivars and 76 landraces,using a 55K single nucleotide polymorphism(SNP)array in multiple environments.A total of 12 stable QTL/SNPs that control different quality traits in this populations in at least two environments under stripe rust stress were identified.Among these 12,three,seven and two QTLs associated with GPC,GSC and WGC were characterized,respectively,and they were located on chromosomes(chr)1B,1D,2A,2B,2D,3B,3D,5D,and 7D with the phenotypic variation explained(PVE)ranging from 4.2 to 10.7%.Compared with the previously reported QTLs/genes,five QTLs(QGsc.sicau-1BL,QGsc.sicau-1DS,QGsc.sicau-2DL.1,QGsc.sicau-2DL.2,and QWgc.sicau-5DL)were potentially novel.KASP markers for the SNPs AX-108770574 and AX-108791420 on chr5D associated with wet gluten concentration were successfully developed.The phenotypes of the cultivars containing the A-allele in AX-108770574and the T-allele in AX-108791420 were extremely significantly(P<0.01)higher than those of the landraces containing the G-or C-allele with respect to the wet gluten concentration in each of the environments.The KASP markers developed and validated in this study could be utilized in molecular breeding aimed at improving the quality of wheat.展开更多
文摘HIP-55(HPK1-interacting protein of 55 ku,又名DBNL,SH3P7,mAbp1)是一种与HPK-1相结合分子量为55 ku,由多个具有蛋白相互作用结构域构成的接头蛋白。其N端含有肌动蛋白结合结构域C端具有SH3结构域。HIP-55能够通过细胞内信号转导调控细胞内吞、迁移、骨架重排等生物功能并且在多种疾病病理生理过程中发挥重要作用。该文将对HIP-55蛋白生物学功能及其胞内信号转导通路参与疾病病理过程分子机制进行综述。
基金supported by National Natural Science Foundations of China[No.81872647]the Natural Science Foundation of the Jiangsu Higher Education Institutions of China[19KJB330007].
文摘Objective The purpose of this study was to investigate the anti-androgenic mechanism of cypermethrin involving coactivators.Methods Mammalian two-hybrid assays were performed to study the effects of cypermethrin on interactions of the androgen receptor(AR)with the coactivators androgen receptor-associated protein70(ARA70)and androgen receptor-associated protein 55(ARA55).Results The results showed that AR–ARA70 and AR–ARA55 interactions were remarkably enhanced by dihydrotestosterone(DHT,P≤0.05).Cypermethrin inhibited DHT-induced AR–ARA70 and AR–ARA55 interactions significantly(P≤0.05).Conclusion The study indicates that cypermethrin exhibits inhibitory effects on AR transcription associated with repression of AR–ARA70 and AR–ARA55 interactions in a ligand-dependent manner.The data show novel anti-androgenic mechanisms of cypermethrin that contribute to male reproductive toxicology.
文摘HIV-1 matrix protein (MA) is a multifunctional structural protein localized on N terminus of Gag precursor p55. MA participates in HIV-1 assembly as membranotropic part of Gag precursor as well as an individual protein spliced from Gag early in infection. MA is found in the nuclei of infected cells and in plasma membrane, the site of virus assembly, in association with viral genome RNA. MA mutated variant M4 which contains two changed amino acids in N-terminal regions is also associated with viral RNA, but it is localized in the nuclear and cytoskeleton fractions but not in the plasma membrane suggesting that the mutant is deprived of membranotropic signal and “sticks” in the nuclei an d cytoskeleton, its previous location sites. These data allow suggesting that MA involved into transmission of viral RNA is transported to plasma membrane by cytoskeleton.
文摘目的探讨中心体相关蛋白55(CEP55)在结直肠癌组织中的表达及其与临床病理特征及预后的关系。方法选取2017年8月至2020年8月在唐山市中医医院确诊为结直肠癌并进行手术治疗的126例患者作为研究对象,收集结直肠癌患者术中切除的结直肠癌组织及癌旁组织(距离癌组织>2 cm)标本各126例。通过qPCR、免疫组化实验检测结直肠癌组织及癌旁组织中CEP55的表达,分析结直肠癌组织中CEP55的表达与临床病理特征的关系,采用Kaplan-Meier法绘制生存曲线,并分析结直肠癌组织中CEP55的表达与患者3年总生存率之间的关系。结果结直肠癌组织中CEP55 m RNA的表达高于癌旁组织【(1.0±0.2)vs.(0.6±0.1)】,差异有统计学意义(P<0.001)。免疫组化结果显示,CEP55在结直肠癌组织中的表达高于癌旁组织,结直肠癌组织中CEP55高表达比例高于癌旁组织(P<0.001)。结直肠癌组织中CEP55的表达与TNM分期有关(P<0.05),与性别、年龄、肿瘤分化程度、肿瘤最大直径、淋巴结转移、肿瘤位置、肿瘤浸润深度无关(P>0.05)。Ⅲ+Ⅳ期结直肠癌患者组织中CEP55的表达评分高于Ⅰ+Ⅱ期结直肠癌患者(P<0.001)。CEP55高表达的结直肠癌患者3年总生存率低于CEP55低表达的结直肠癌患者(70.3%vs.88.6%),差异有统计学意义(Log-rankχ^(2)=4.715,P=0.031)。结论CEP55在结直肠癌组织中高表达,与TNM分期有关,可能可以作为结直肠癌的潜在预后生物标志物。
文摘The TicS5 （Translocon at the inner envelope membrane of chloroplasts, 55 kDa） protein was identified in pea as a putative regulator, possibly linking chloroplast protein import to the redox state of the photosynthetic machinery. Two Tic55 homologs have been proposed to exist in Arabidopsis： atTic55-11 and AtPTC52 （Protochlorophyllide-dependent Trans- Iocon Component, 52 kDa; has also been called atTic55-1V）. Our phylogenetic analysis shows that attic55-11 is an ortholog of psTic55 from pea （Pisum sativurn）, and that AtPTC52 is a more distant homolog of the two. AtPTC52 was included in this study to rule out possible functional links between the proteins in Arabidopsis. No detectable mutant phenotypes were found in two independent T-DNA knockout mutant plant lines for each Arabidopsis protein, when compared with wild- type： visible appearance, chlorophyll content, photosynthetic performance, and chloroplast protein import, for example, were all normal. Both wild-type and tic55-11 mutant chloroplasts exhibited deficient protein import when treated with diethylpyrocarbonate, indicating that Tic55 is not the sole target of this reagent in relation to protein import. Furthermore, ptc52 mutant chloroplasts were not defective with respect to pPORA import, which was previously reported to involve PTC52 in barley. Thus, we conclude that atTic55-11 and AtPTC52 are not strictly required for functional protein import in Arabidopsis.
文摘目的探索中心体蛋白55(centrosome protein 55,CEP55)对膀胱癌细胞增殖能力的影响及相关分子机制。方法利用蛋白免疫印迹检测正常膀胱组织细胞(SV-HUC-1)与膀胱癌细胞(T24)CEP55、H3K9 me3的表达量。将膀胱癌细胞T24分为实验组和对照组,实验组细胞转染siRNA-CEP55,对照组细胞转染siRNA-MOCK。利用蛋白免疫印迹实验检测各组细胞CEP55、H3K9、H3K9me3的表达量,利用CCK8实验检测各组细胞的增殖能力。结果蛋白免疫印迹实验结果表明,膀胱癌T24细胞CEP55表达量为0.83±0.15,H3K9me3表达量为1.01±0.19。膀胱上皮SV-HUC-1细胞CEP55表达量为0.35±0.09,H3K9me3表达量为0.44±0.10,膀胱癌细胞CEP55、H3K9me3的表达均高于正常膀胱细胞(均P<0.05)。siRNA-CEP55成功降低膀胱癌细胞T24 CEP55的表达。实验组T24细胞吸光度为1.109±0.105,明显低于对照组细胞的2.208±0.104,CEP55低表达会降低T24细胞的增殖能力(P<0.05)。蛋白免疫印迹结果显示实验组T24细胞H3K9变化不明显,H3K9me3表达(P<0.05)明显下降。结论CEP55能通过降低H3K9的三甲基化来抑制膀胱癌细胞的增殖能力。
基金supported by the National Key Research and Development Program of China(2017YFD0100900,2016YFD0102000 and 2016YFD0100100)the International Science and Technology Cooperation and Exchanges Programs of Science and Technology Department of Sichuan Province,China(2019YFH0063)the Sichuan Science and Technology Program,China(2022ZDZX0014)。
文摘Understanding the genetic basis of quality-related traits contributes to the improvement of grain protein concentration(GPC),grain starch concentration(GSC),and wet gluten concentration(WGC)in wheat.In this study,a genome-wide association study(GWAS)based on a mixed linear model(MLM)was performed on 236 wheat accessions,including 160 cultivars and 76 landraces,using a 55K single nucleotide polymorphism(SNP)array in multiple environments.A total of 12 stable QTL/SNPs that control different quality traits in this populations in at least two environments under stripe rust stress were identified.Among these 12,three,seven and two QTLs associated with GPC,GSC and WGC were characterized,respectively,and they were located on chromosomes(chr)1B,1D,2A,2B,2D,3B,3D,5D,and 7D with the phenotypic variation explained(PVE)ranging from 4.2 to 10.7%.Compared with the previously reported QTLs/genes,five QTLs(QGsc.sicau-1BL,QGsc.sicau-1DS,QGsc.sicau-2DL.1,QGsc.sicau-2DL.2,and QWgc.sicau-5DL)were potentially novel.KASP markers for the SNPs AX-108770574 and AX-108791420 on chr5D associated with wet gluten concentration were successfully developed.The phenotypes of the cultivars containing the A-allele in AX-108770574and the T-allele in AX-108791420 were extremely significantly(P<0.01)higher than those of the landraces containing the G-or C-allele with respect to the wet gluten concentration in each of the environments.The KASP markers developed and validated in this study could be utilized in molecular breeding aimed at improving the quality of wheat.
基金supported by the Scientific Research Foundation of Henan UniversityHenan Province+1 种基金China(No.2012YBZR020)the National Natural Science Foundation of China(No.U1304802)
