Summary Five subfamilies of the Cerambycidate: Lamiinae, Cerambycinae, Lepturinae (including Necydalini and Apatophysis), Aseminae (including S pondylis), and Prioninae (including Parandra) are recognized in this pape...Summary Five subfamilies of the Cerambycidate: Lamiinae, Cerambycinae, Lepturinae (including Necydalini and Apatophysis), Aseminae (including S pondylis), and Prioninae (including Parandra) are recognized in this paper, All the evolutionary evidences provided in the present research indicate that these subfamilies may be arranged in order from the oldest to the youngest as follows: (1) Lamiinae, Prioninae, Cerambycinae, Lepturinae, Aseminae; or (2) Lamiinae, Cerambycinae, Prioninae, Lepturinae, Aseminae. The possible phylogenetic relationships among the subfamilies are also given.展开更多
Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymera...Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymerase chain reaction(PCR) and conserved primer walking approaches.The results showed that the entire mitogenome is 15418 bp long with 74.48% A+T content.The positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available.All protein-coding genes start with the ATN codon that is a typical initiation codon in insects.All transfer RNAs(tRNAs) were predicted to form the standard clover-leaf structure,except for tRNA Ser(AGN),which lacks the dihydrouridine(DHU) arm.The most unusual feature that was found was the use of TCT as the tRNA Ser(AGN) anticodon instead of the GCT that is used in most other arthropods.The lack of tandem repeat motif in the 735 bp long A+T-rich region was another unusual feature of the B.lineolata mitogenome.The short,highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A+T-rich region of the B.lineolata(order Coleoptera) mitogenome.The sequence and annotation of the mitogenome of B.lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of long-horned beetles.The B.lineolata mitogenome sequence has been deposited in GenBank(http://www.ncbi.nlm.nih.gov/Genbank/) under accession number JN986793.展开更多
The yellow meal worm （Tenebrio molitor L.） is an important resource insect typically used as animal feed additive. It is also widely used for biological research. The first complete mitochondrial genome of T. rnolit...The yellow meal worm （Tenebrio molitor L.） is an important resource insect typically used as animal feed additive. It is also widely used for biological research. The first complete mitochondrial genome of T. rnolitor was determined for the first time by long PCR and conserved primer walking approaches. The results showed that the entire mitogenome of T. molitor was 15 785 bp long, with 72.35% A＋T content [deposited in GenBank with accession number KF418153]. The gene order and orientation were the same as the most common type suggested as ancestral for insects. Two protein-coding genes used atypical start codons （CTA in ND2 and AAT in COX1）, and the remaining 11 protein-coding genes started with a typical insect initiation codon ATN. All tRNAs showed standard clover-leaf structure, except for tRNASer （AGN）, which lacked a dihydrouridine （DHU） arm. The newly added T. molitor mitogenome could provide information for future studies on yellow meal worm.展开更多
Mosquitoes are of great medical significance as vectors of many deadly diseases.Mitogenomes have been widely used in phylogenetic studies,but mitogenome knowledge within the family Culicidae is limited,and Culicidae p...Mosquitoes are of great medical significance as vectors of many deadly diseases.Mitogenomes have been widely used in phylogenetic studies,but mitogenome knowledge within the family Culicidae is limited,and Culicidae phylogeny is far from resolved.In this study,we surveyed the mitogenomes of 149 Culicidae species,including 7 newly sequenced species.Comparative analysis of 149 mosquito mitogenomes shows gene composition and order to be identical to that of an ancestral insect,and the AT bias,length variation,and codon usage are all consistent with that of other reported Dipteran mitogenomes.Phylogenetic analyses based on the DNA sequences of the 13 protein-coding genes from the 149 species robustly support the monophyly of the subfamily Anophelinae and the tribes Aedini,Culicini,Mansonini,Sabethini,and Toxorhynchitini.To resolve ambiguous relationships between clades within the subfamily Culicinae,we performed topological tests and show that Aedini is a sister to Culicini and that Uranotaenini is a sister to(Mansonini)+(Toxorhynchitini+Sabethini).In addition,we estimated divergence times using a Bayesian relaxation clock based on the sequence data and 3 fossil calibration points.The results show mosquitoes diverged during the Early Jurassic with massive Culicinae radiations during the Cretaceous,coincident with the emergence of angiosperms and the burst of mammals and birds.Overall,this study,which uses the largest number of Culicidae mitogenomes sequenced to date,comprehensively reveals the mitogenome characteristics and mitogenome-based phylogeny and divergence times of Culicidae,providing information for further studies on the mitogenome,phylogeny,evolution,and taxonomic revision of Culicidae.展开更多
文摘Summary Five subfamilies of the Cerambycidate: Lamiinae, Cerambycinae, Lepturinae (including Necydalini and Apatophysis), Aseminae (including S pondylis), and Prioninae (including Parandra) are recognized in this paper, All the evolutionary evidences provided in the present research indicate that these subfamilies may be arranged in order from the oldest to the youngest as follows: (1) Lamiinae, Prioninae, Cerambycinae, Lepturinae, Aseminae; or (2) Lamiinae, Cerambycinae, Prioninae, Lepturinae, Aseminae. The possible phylogenetic relationships among the subfamilies are also given.
基金supported by the National Ministry of Forestry of China (2009-4-37)the Science and Technology Committee of Yunnan Province,China (2011FB141)
文摘Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymerase chain reaction(PCR) and conserved primer walking approaches.The results showed that the entire mitogenome is 15418 bp long with 74.48% A+T content.The positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available.All protein-coding genes start with the ATN codon that is a typical initiation codon in insects.All transfer RNAs(tRNAs) were predicted to form the standard clover-leaf structure,except for tRNA Ser(AGN),which lacks the dihydrouridine(DHU) arm.The most unusual feature that was found was the use of TCT as the tRNA Ser(AGN) anticodon instead of the GCT that is used in most other arthropods.The lack of tandem repeat motif in the 735 bp long A+T-rich region was another unusual feature of the B.lineolata mitogenome.The short,highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A+T-rich region of the B.lineolata(order Coleoptera) mitogenome.The sequence and annotation of the mitogenome of B.lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of long-horned beetles.The B.lineolata mitogenome sequence has been deposited in GenBank(http://www.ncbi.nlm.nih.gov/Genbank/) under accession number JN986793.
基金This work was supported by grant from the Science and Technology Committee of Yunnan Province （2011 FB141 ） We thank Dr. Zhong-Bao ZHAO for helpful suggestions during manuscript preparation.
文摘The yellow meal worm （Tenebrio molitor L.） is an important resource insect typically used as animal feed additive. It is also widely used for biological research. The first complete mitochondrial genome of T. rnolitor was determined for the first time by long PCR and conserved primer walking approaches. The results showed that the entire mitogenome of T. molitor was 15 785 bp long, with 72.35% A＋T content [deposited in GenBank with accession number KF418153]. The gene order and orientation were the same as the most common type suggested as ancestral for insects. Two protein-coding genes used atypical start codons （CTA in ND2 and AAT in COX1）, and the remaining 11 protein-coding genes started with a typical insect initiation codon ATN. All tRNAs showed standard clover-leaf structure, except for tRNASer （AGN）, which lacked a dihydrouridine （DHU） arm. The newly added T. molitor mitogenome could provide information for future studies on yellow meal worm.
基金supported by the following:The National Natural Science Foundation of China(31872262,31672363)National Key Program of Science and Technology Foundation Work of China(2015FY210300)Science and Technology Research Program of Chongqing Education Commission of China(KJQN202000504,KJQN202200566).
文摘Mosquitoes are of great medical significance as vectors of many deadly diseases.Mitogenomes have been widely used in phylogenetic studies,but mitogenome knowledge within the family Culicidae is limited,and Culicidae phylogeny is far from resolved.In this study,we surveyed the mitogenomes of 149 Culicidae species,including 7 newly sequenced species.Comparative analysis of 149 mosquito mitogenomes shows gene composition and order to be identical to that of an ancestral insect,and the AT bias,length variation,and codon usage are all consistent with that of other reported Dipteran mitogenomes.Phylogenetic analyses based on the DNA sequences of the 13 protein-coding genes from the 149 species robustly support the monophyly of the subfamily Anophelinae and the tribes Aedini,Culicini,Mansonini,Sabethini,and Toxorhynchitini.To resolve ambiguous relationships between clades within the subfamily Culicinae,we performed topological tests and show that Aedini is a sister to Culicini and that Uranotaenini is a sister to(Mansonini)+(Toxorhynchitini+Sabethini).In addition,we estimated divergence times using a Bayesian relaxation clock based on the sequence data and 3 fossil calibration points.The results show mosquitoes diverged during the Early Jurassic with massive Culicinae radiations during the Cretaceous,coincident with the emergence of angiosperms and the burst of mammals and birds.Overall,this study,which uses the largest number of Culicidae mitogenomes sequenced to date,comprehensively reveals the mitogenome characteristics and mitogenome-based phylogeny and divergence times of Culicidae,providing information for further studies on the mitogenome,phylogeny,evolution,and taxonomic revision of Culicidae.
