The Chloroplast Genome of Cerasus Campanulata Diverges from Other Prunoideae Genomes

Cerasus Campanulata is one of several species belonging to the Prunoideae focke,a subfamily of the flowering plant Rosaceae.We investigated the details of its chloroplast genome which may reveal its genus independent of morphological determination.Here,we determined the complete chloroplast(cp)genome sequence of C.campanulata and performed sequence analysis to reveal the presence of 18 forward repeats,20 palindrome repeats,2 complement repeats,4 reverse repeats and 93 simple sequence repeats(SSRs).We additionally performed a comparative study of C.campanulata and seven other Prunoideae focke species.Then,maximum parsimony(MP)and maximum likelihood(ML)phylogenetic analyses were carried out in the little part of Rosaceae,respectively.The results strongly support a position of C.campanulata as a member of the Cerasus in the Rosaceae family.Moreover,the complete cp genome can be used for plant phylogenetic and evolutionary studies that will provide insight into the degree of gene conservation.

武夷红樱组培快繁关键技术

组培快繁技术可有效提高武夷红樱(Cerasus campanulatavar.wuyiensis)种苗繁殖速度。本研究通过正交试验设计探讨基本培养基、不同植物生长调节物质对武夷红樱外植体诱导、芽苗增殖及生根培养等关键技术环节的影响。结果表明:武夷红樱外植体初代培养最佳诱导培养基为:WPM+6-BA 1.5 mg/L+NAA 0.2 mg/L,诱导率达91.33%,平均芽高1.92 cm;芽苗最佳继代增殖培养基为:WPM+6-BA 1.0 mg/L+KT 1.0 mg/L+NAA 0.3 mg/L,增殖系数3.06,芽苗平均高度1.90 cm;最佳的生根培养基为:1/2WPM+NAA 0.5 mg/L+IBA 0.4 mg/L,生根率92.73%,平均根条数达4.54条。建立起的武夷红樱组培快繁生产技术体系可为种苗工厂化生产提供技术支撑,加快武夷红樱优质种苗的市场化推广。