Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 ...Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 umol/L Hg, and 20 umol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.展开更多
High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerge...High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aquatic plant Ceratophyllum demersum.A comparative study was conducted by setting four treatments:open-circuit SMFC(Control),closed-circuit SMFC(SMFC-c),open-circuit SMFC with C.demersum(Plant),and closed-circuit SMFC with C.demersum(P-SMFC-c).The electrochemical parameters were documented to illustrate the bio-electrochemical characteristics of SMFC-c and P-SMFC-c.Removal pathways of NO_3^- in different treatments were studied by adding quantitative^(15)NO_3^- to water column.The results showed that the cathodic reaction in SMFC-c was mainly catalyzed by aerobic organisms attached on the cathode,including algae,Pseudomonas,Bacillus,and Albidiferax.The oxygen secreted by plants significantly improved the power generation of SMFC-c.Both electrogenesis and plants enhanced the complete removal of NO_3^- from the sediment–water system.The complete removal rates of added^(15)N increased by 17.6% and 10.2% for SMFC-c and plant,respectively,when compared with control at the end of experiment.The electrochemical/heterotrophic and aerobic denitrification on cathodes mainly drove the higher reduction of NO_3^- in SMFC-c and plant,respectively.The coexistence of electrogenesis and plants further increased the complete removal of NO_3^- with a rate of 23.1%.The heterotrophic and aerobic denitrifications were simultaneously promoted with a highest abundance of Flavobacterium,Bacillus,Geobacter,Pseudomonas,Rhodobacter,and Arenimonas on the cathode.展开更多
Objective:To evaluate the effect of some different water temperatures on growth of aquatic plants (Salvinia natans andCeratophyllum demersum). <br> Methods:The aquatic plants were brought from Shatt Al-Arab Rive...Objective:To evaluate the effect of some different water temperatures on growth of aquatic plants (Salvinia natans andCeratophyllum demersum). <br> Methods:The aquatic plants were brought from Shatt Al-Arab River in 2016. Equal weights of aquatic plants were aquacultured in aquaria, and were exposed to three different temperatures ( 12, 22 and 32°C). <br> Results: The results showed that the two plants did not show significant differences with respect to their effects on pH and electrical conductivity values. Time and temperature did not affect the values of pH and electrical conductivity. The values of dissolved oxygen was significantly influenced with variation of time and temperature, while the two plants did not have significant differences on dissolved oxygen values, nitrate ion concentration and was not significantly influenced with variation of plant species or temperature or time. Plant species and temperature significantly affected phosphate ion concentration, while the time did not significantly influence the concentration of phosphate ion. Chlorophyll a content and biomass were significantly influenced with the variation of plant species, and temperature . <br> Conclusions:Aquatic plants has a species specific respond to temperatures change in their environment. Water plant,Ceratophyllum demersum is more tolerant to temperatures change thanSalvinia natans.展开更多
Fatty, phenolic and hydroxy acids in aqueous leachates of three submerged macrophytes 〔Ceratophyllum demersum L., Vallisneria spiralis L., Hydrilla verticillata (L. f.) Royle〕 were analyzed using GC-MS in shallow fr...Fatty, phenolic and hydroxy acids in aqueous leachates of three submerged macrophytes 〔Ceratophyllum demersum L., Vallisneria spiralis L., Hydrilla verticillata (L. f.) Royle〕 were analyzed using GC-MS in shallow freshwater. Succinic acid, oxalic acid, cis-aconitic acid, lactic acid and ferulic acid were main components. The content of cis-aconitic acid was more than 18% in H. verticillata but less than 2% in C. demersum and V. spiralis. The content of phenolic acid accounted for 8.85% in C. demersum but 1.23% and 0.63% in V. spiralis and H. verticillata.展开更多
基金supported financially by Department of Science and Technology, New Delhi, India under the SERC-DST fasttrack scheme.
文摘Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/L Cd, 5 umol/L Hg, and 20 umol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.
基金supported by the National Key Research and Development Plan of China (No. 2016YFC0500403-03)the Science and Technology Service Network Initiative of the Chinese Academy of Sciences (No. KFJ-STS-ZDTP-038)
文摘High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aquatic plant Ceratophyllum demersum.A comparative study was conducted by setting four treatments:open-circuit SMFC(Control),closed-circuit SMFC(SMFC-c),open-circuit SMFC with C.demersum(Plant),and closed-circuit SMFC with C.demersum(P-SMFC-c).The electrochemical parameters were documented to illustrate the bio-electrochemical characteristics of SMFC-c and P-SMFC-c.Removal pathways of NO_3^- in different treatments were studied by adding quantitative^(15)NO_3^- to water column.The results showed that the cathodic reaction in SMFC-c was mainly catalyzed by aerobic organisms attached on the cathode,including algae,Pseudomonas,Bacillus,and Albidiferax.The oxygen secreted by plants significantly improved the power generation of SMFC-c.Both electrogenesis and plants enhanced the complete removal of NO_3^- from the sediment–water system.The complete removal rates of added^(15)N increased by 17.6% and 10.2% for SMFC-c and plant,respectively,when compared with control at the end of experiment.The electrochemical/heterotrophic and aerobic denitrification on cathodes mainly drove the higher reduction of NO_3^- in SMFC-c and plant,respectively.The coexistence of electrogenesis and plants further increased the complete removal of NO_3^- with a rate of 23.1%.The heterotrophic and aerobic denitrifications were simultaneously promoted with a highest abundance of Flavobacterium,Bacillus,Geobacter,Pseudomonas,Rhodobacter,and Arenimonas on the cathode.
文摘Objective:To evaluate the effect of some different water temperatures on growth of aquatic plants (Salvinia natans andCeratophyllum demersum). <br> Methods:The aquatic plants were brought from Shatt Al-Arab River in 2016. Equal weights of aquatic plants were aquacultured in aquaria, and were exposed to three different temperatures ( 12, 22 and 32°C). <br> Results: The results showed that the two plants did not show significant differences with respect to their effects on pH and electrical conductivity values. Time and temperature did not affect the values of pH and electrical conductivity. The values of dissolved oxygen was significantly influenced with variation of time and temperature, while the two plants did not have significant differences on dissolved oxygen values, nitrate ion concentration and was not significantly influenced with variation of plant species or temperature or time. Plant species and temperature significantly affected phosphate ion concentration, while the time did not significantly influence the concentration of phosphate ion. Chlorophyll a content and biomass were significantly influenced with the variation of plant species, and temperature . <br> Conclusions:Aquatic plants has a species specific respond to temperatures change in their environment. Water plant,Ceratophyllum demersum is more tolerant to temperatures change thanSalvinia natans.
文摘Fatty, phenolic and hydroxy acids in aqueous leachates of three submerged macrophytes 〔Ceratophyllum demersum L., Vallisneria spiralis L., Hydrilla verticillata (L. f.) Royle〕 were analyzed using GC-MS in shallow freshwater. Succinic acid, oxalic acid, cis-aconitic acid, lactic acid and ferulic acid were main components. The content of cis-aconitic acid was more than 18% in H. verticillata but less than 2% in C. demersum and V. spiralis. The content of phenolic acid accounted for 8.85% in C. demersum but 1.23% and 0.63% in V. spiralis and H. verticillata.
