OBJECTIVE: To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion(MCAO).METHODS: A rat model of ...OBJECTIVE: To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion(MCAO).METHODS: A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group(Control), a sham-operated group(Sham), an ischemic group(MCAO), and an ischemic group treated with Ginkgo biloba leaf extract(MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.RESULTS: Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group(P < 0.05, P < 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine(Ala), Isoleucine(Ile), Glutamic acid(Glu), Serine(Ser), Valine(Val), Phenylalanine(Phe), Proline(Pro),Threonine(Thr), Lysine(Lys), Tyrosine(Tyr), Hydroxyproline(Hyp), Arginine(Arg), Leucine(Leu),Tryptophan(Trp), and Glycine(Gly) were increased(P < 0.001, P < 0.05), while levels of Gln and Tau were decreased(P < 0.001, P < 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.CONCLUSION: Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex.展开更多
Objective: To investigate the role of endothelin 1 (ET 1) in development of ischemic brain damage after subarachnoid hemorrhage (SAH), and the protective effect of Ginkgo biloba extract (GBE) on it. Methods: Noncr...Objective: To investigate the role of endothelin 1 (ET 1) in development of ischemic brain damage after subarachnoid hemorrhage (SAH), and the protective effect of Ginkgo biloba extract (GBE) on it. Methods: Noncraniotomy SAH models of Wistar rat were divided into SAH group and GBE treated group, the diameter of basilar artery (BA), dynamic changes of regional cerebral blood flow (rCBF) and ET 1 content of intracranial plasma within 24 hours after SAH of both groups were determined. And pathological examination of CA1 region of hippocampus was performed 3 days later. Results: rCBF decreased and ET 1 content increased obviously and steadily in 24 hours after SAH. Spasm of BA occurred half an hour after SAH and neurons of hippocampus CA1 region were damaged severely. GBE could antagonize the above mentioned pathological changes effectively. Conclusion:Increase of ET 1 is an important factor leading to ischemic brain damage after SAH. GBE exerts its protective effect by antagonizing pathological increase of ET 1.展开更多
基金supported by the Key Program of National Natural Science Foundation of China (81330086)the General Program of National Natural Science Foundation of China (81573726, 81403210)the Fundamental Research Funds for the Central Universities (No. 2017-JYB-JS-054)
文摘OBJECTIVE: To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion(MCAO).METHODS: A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group(Control), a sham-operated group(Sham), an ischemic group(MCAO), and an ischemic group treated with Ginkgo biloba leaf extract(MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.RESULTS: Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group(P < 0.05, P < 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine(Ala), Isoleucine(Ile), Glutamic acid(Glu), Serine(Ser), Valine(Val), Phenylalanine(Phe), Proline(Pro),Threonine(Thr), Lysine(Lys), Tyrosine(Tyr), Hydroxyproline(Hyp), Arginine(Arg), Leucine(Leu),Tryptophan(Trp), and Glycine(Gly) were increased(P < 0.001, P < 0.05), while levels of Gln and Tau were decreased(P < 0.001, P < 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.CONCLUSION: Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex.
文摘Objective: To investigate the role of endothelin 1 (ET 1) in development of ischemic brain damage after subarachnoid hemorrhage (SAH), and the protective effect of Ginkgo biloba extract (GBE) on it. Methods: Noncraniotomy SAH models of Wistar rat were divided into SAH group and GBE treated group, the diameter of basilar artery (BA), dynamic changes of regional cerebral blood flow (rCBF) and ET 1 content of intracranial plasma within 24 hours after SAH of both groups were determined. And pathological examination of CA1 region of hippocampus was performed 3 days later. Results: rCBF decreased and ET 1 content increased obviously and steadily in 24 hours after SAH. Spasm of BA occurred half an hour after SAH and neurons of hippocampus CA1 region were damaged severely. GBE could antagonize the above mentioned pathological changes effectively. Conclusion:Increase of ET 1 is an important factor leading to ischemic brain damage after SAH. GBE exerts its protective effect by antagonizing pathological increase of ET 1.
文摘目的:观察静脉溶栓后应用银杏叶提取物(Ginkgo biloba extract,EGB)-761联合盐酸替罗非班注射液治疗急性脑梗死的疗效及对血清同型半胱氨酸(homocysteine,Hcy)、血管内皮生长因子(vascular endothelial growth factor,VEGF)水平和活化凝血因子VII/凝血因子VII抗原(activated coagulation factor VII/coagulation factor VII antigen,FVIIa/FVIIAg)比值的影响。方法:选取河南大学第一附属医院2022年1月至12月收治的368例急性脑梗死患者为研究对象,根据患者入院顺序采用奇偶数分组法分为观察组(n=188)和对照组(n=180)。2组均给予静脉溶栓,对照组给予盐酸替罗非班注射液治疗,观察组在对照组基础上联合EGB-761治疗。比较2组Hcy、FVIIa/FVIIAg比值、VEGF、趋化因子CXC配体(chemokine CXC ligand,CXCL-12)、大脑中动脉血流指标的差异,评估2组斑块数量、面积及巴塞尔指数(Barthel index,BI)、弥散加权成像和Alberta卒中项目早期CT评分(diffusion-weighted imaging and early CT in the Alberta Stroke Program Score,DWI-ASPECTS)水平,统计2组疗效。结果:治疗前,2组Hcy、FVIIa/FVIIAg比值、VEGF、CXCL-12比较,差异均无统计学意义(均P>0.05)。与本组治疗前比较,治疗后2组Hcy、CXCL-12水平和FVIIa/FVIIAg比值均下降,且与对照组比较,观察组更低(均P<0.01);2组VEGF均升高,且与对照组比较,观察组更高(均P<0.01)。治疗前,2组斑块数量、面积、大脑中动脉血流指标比较,差异均无统计学意义(均P>0.05)。与本组治疗前比较,治疗后2组斑块数量、面积及大脑中动脉搏动指数(pulsatility index,PI)均下降,且与对照组比较,观察组更低(均P<0.01)。2组大脑中动脉平均血流速度(mean blood flow velocity,Vm)、舒张末期血流速度(enddiastolic blood flow velocity,Vd)均升高,且与对照组比较,观察组更高(均P<0.01)。观察组总有效率为95.21%(179/188),高于对照组的89.44%(161/180),差异有统计学意义(P<0.05)。治疗前,2组BI、DWI-ASPECTS比较,差异均无统计学意义(均P>0.05)。与本组治疗前比较,治疗后2组BI、DWI-ASPECTS均升高,且与对照组比较,观察组更高(均P<0.01)。结论:静脉溶栓后应用EGB-761联合盐酸替罗非班注射液治疗可通过调节患者的血清Hcy和VEGF表达及FVIIa/FVIIAg比值,增加脑血流量,减少颈动脉斑块,从而改善急性脑梗死患者的预后。
