Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorl...Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.展开更多
ObjectiveTo understand the pathogenetic role and clinical significance of interleukin 6(IL -6),tumor necrosis factor(TNF)and soluble interleukin -2receptor(sIL -2R)in patients with cerebral infarctio n(CI ).MethodsThe...ObjectiveTo understand the pathogenetic role and clinical significance of interleukin 6(IL -6),tumor necrosis factor(TNF)and soluble interleukin -2receptor(sIL -2R)in patients with cerebral infarctio n(CI ).MethodsThe IL -6,TNF and sIL -2R levels were measured in 46CI patient s with ELISA.ResultsThe serum IL -6,TNF and sIL -2R levels of CI group were much higher than those of controls group .The variation of IL -6,TNF and sIL -2R levels was c losely related to the size of cerebra l infarction.The serum IL -6,TNF and sIL -2R levels of recovery p eriod were much lower than those of acute period in patients with CI .Conclusions The IL -6,TNF and sIL -2R take part in the pathologi c process after CI .The measurement o f IL -6,TNF and sIL -2R is useful in determining the size of cerebral infarction.展开更多
基金supported by a grant from the National Natural Science Foundation of China,No.30972766,31170852,81001322,81172795,81173048the Specialized Research Fund for the Doctoral Program of Colleges and Universities,No.20094402110004
文摘Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.
文摘ObjectiveTo understand the pathogenetic role and clinical significance of interleukin 6(IL -6),tumor necrosis factor(TNF)and soluble interleukin -2receptor(sIL -2R)in patients with cerebral infarctio n(CI ).MethodsThe IL -6,TNF and sIL -2R levels were measured in 46CI patient s with ELISA.ResultsThe serum IL -6,TNF and sIL -2R levels of CI group were much higher than those of controls group .The variation of IL -6,TNF and sIL -2R levels was c losely related to the size of cerebra l infarction.The serum IL -6,TNF and sIL -2R levels of recovery p eriod were much lower than those of acute period in patients with CI .Conclusions The IL -6,TNF and sIL -2R take part in the pathologi c process after CI .The measurement o f IL -6,TNF and sIL -2R is useful in determining the size of cerebral infarction.
