Polymorphism in two types of Cereus peruvianus, short and long spines clones, and its F1 hybrids cultivated in Indonesia were detected by RAPD （Random Amplified Polymorphic DNA） markers. High amount of mucilage （ge...Polymorphism in two types of Cereus peruvianus, short and long spines clones, and its F1 hybrids cultivated in Indonesia were detected by RAPD （Random Amplified Polymorphic DNA） markers. High amount of mucilage （gelling polysaccharides） present in C. peruvianus was a major obstacle in isolating good quality genomic DNA. To obtain good quality DNA, the CTAB （Cetyl Trimethyl Ammonium Bromide） methode was modified. Out of 17 primers were used, and two primers OPN-05 and OPM-10 have specific loci, OPN-05550, OPN-05800 and OPM-10650, linked with spines types in parents clones. Those can be used as molecular marker for spines type. Seventeen primers were used generated 113 loci, of which 65 loci were polymorphic in parental clones and 132 loci, of which 93 loci were polymorphic in F1 plants. Dendrogram generated by Jaccard coefficient showed that parents＇ clones had lower genetic diversity than F1 plants. At 72% similarity, all of long or short spine parent clones grouped in one cluster according to its size of spines. At that time F1 plants were separately grouped. None ofF1 hybrid plants grouped with its common female parents.展开更多
文摘Polymorphism in two types of Cereus peruvianus, short and long spines clones, and its F1 hybrids cultivated in Indonesia were detected by RAPD （Random Amplified Polymorphic DNA） markers. High amount of mucilage （gelling polysaccharides） present in C. peruvianus was a major obstacle in isolating good quality genomic DNA. To obtain good quality DNA, the CTAB （Cetyl Trimethyl Ammonium Bromide） methode was modified. Out of 17 primers were used, and two primers OPN-05 and OPM-10 have specific loci, OPN-05550, OPN-05800 and OPM-10650, linked with spines types in parents clones. Those can be used as molecular marker for spines type. Seventeen primers were used generated 113 loci, of which 65 loci were polymorphic in parental clones and 132 loci, of which 93 loci were polymorphic in F1 plants. Dendrogram generated by Jaccard coefficient showed that parents＇ clones had lower genetic diversity than F1 plants. At 72% similarity, all of long or short spine parent clones grouped in one cluster according to its size of spines. At that time F1 plants were separately grouped. None ofF1 hybrid plants grouped with its common female parents.
