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Effect of NaNO_3 Concentrations on the Growth and Fatty Acid Compositions of Nitzschia closterium and Chaetoceros gracilis* 被引量:1
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作者 梁英 麦康森 孙世春 《Marine Science Bulletin》 CAS 2003年第1期69-74,共6页
Fatty acid compositions of two strains of marine diatoms Nitzschia closterium ( MACC B222 ) and Chaetoceros gracilis ( MACC B13 ) have been examined. The microalgae have been grown at different initial NaNO3 concentr... Fatty acid compositions of two strains of marine diatoms Nitzschia closterium ( MACC B222 ) and Chaetoceros gracilis ( MACC B13 ) have been examined. The microalgae have been grown at different initial NaNO3 concentrations ( 75, 375, 750, 1 125, 1 500, 1 875 mg/L ) and harvested at the late exponential phase. The results by one factor analysis of variance show that the NaNO3 concentrations have no significant influence on the relative growth rate of two strains of marine diatoms; the influence of NaNO3 concentrations on the fatty acids differs from species to species. The major fatty acids of two --diatom strains are 14:0, 16:0, 16:1n7 and 20:5n3 ( EPA ) , B13 also has high percentage -of 20:4n6 ( 6.0 ~ 9.1% ). 展开更多
关键词 Nitzschia closterium chaetoceros gracilis NaNO3 relative growth rate fatty acid
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Effect of Growth Phase on the Fatty Acid Compositions of Four Species of Marine Diatoms 被引量:2
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作者 LIANGYing MAIKangsen 《Journal of Ocean University of China》 SCIE CAS 2005年第2期157-162,共6页
The fatty acid compositions of four species of marine diatoms (Chaetoceros gracilis MACC/B13, Cylindrotheca fusiformis MACC/B211, Phaeodactylum tricornutum MACC/B221 and Nitzschia closterium MACC/B222), cultivated at ... The fatty acid compositions of four species of marine diatoms (Chaetoceros gracilis MACC/B13, Cylindrotheca fusiformis MACC/B211, Phaeodactylum tricornutum MACC/B221 and Nitzschia closterium MACC/B222), cultivated at 22 ℃± 1 ℃ with the salinity of 28 in f/2 medium and harvested in the exponential growth phase, the early stationary phase and the late stationary phase, were determined. The results showed that growth phase has significant effect on most fatty acid contents in the four species of marine diatoms. The proportions of 16:0 and 16:1n-7 fatty acids increased while those of 16:3n-4 and eicosapentaenoic acid (EPA) decreased with increasing culture age in all species studied. The subtotal of saturated fatty acids (SFA) increased with the increasing culture age in all species with the exception of B13. The subtotal of monounsaturated fatty acids (MUFA) increased while that of polyunsaturated fatty acids (PUFA) decreased with culture age in the four species of marine diatoms. MUFA reached their lowest value in the exponential growth phase, whereas PUFA reached their highest value in the same phase. 展开更多
关键词 chaetoceros gracilis Cylindrotheca fusiformis Phaeodactylum tricornutum Nitzschia closterium growth phase fatty acid
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氮、磷供给水平对纤细角毛藻生长及细胞物质组成的影响
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作者 周泽鹏 周晓见 +2 位作者 宋雨晴 刘青 靳翠丽 《水产养殖》 CAS 2020年第10期24-29,共6页
纤细角毛藻(Chaetoceros gracilis)具有丰富的营养组成,是重要的生物饵料微藻。该试验以f/2培养基中标准的氮磷供给水平为基准,进行不同氮磷供给水平对纤细角毛藻生长及细胞物质组成的研究。结果表明,基准浓度的1/2倍及以下的低氮供给... 纤细角毛藻(Chaetoceros gracilis)具有丰富的营养组成,是重要的生物饵料微藻。该试验以f/2培养基中标准的氮磷供给水平为基准,进行不同氮磷供给水平对纤细角毛藻生长及细胞物质组成的研究。结果表明,基准浓度的1/2倍及以下的低氮供给使纤细角毛藻的生物量减少严重,而基准浓度的2倍、3倍高氮供应下仍与基准氮供给相差不大。高于基准磷浓度供给促进纤细角毛藻的生长,其中基准磷浓度的3/2倍最优。在提高纤细角毛藻生物量产量方面,改变磷供给浓度比氮更有效。在细胞物质组成方面,基准浓度的3/2-3倍的高氮供给是提升细胞蛋白质含量的有效手段,而磷浓度变化对蛋白质含量的影响较低。细胞总糖含量的提升可通过降低磷供给水平(1/8-1倍基准浓度)实现。降低氮供应至基准浓度的1/8~1/4倍或者降低磷供应至基准浓度的1/8~1/4倍的寡营养条件,均能有效提高细胞脂肪含量,其中1/8倍的磷供给浓度对于提高脂肪含量效果最好。综合而言,纤细角毛藻的生长和细胞物质组成受磷营养供给的影响大于氮供给。 展开更多
关键词 纤细角毛藻(chaetoceros gracilis) 氮磷供给 生长 细胞物质组成
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