Liver depression and qi stagnation type liver cancer accounts for a high proportion of primary liver cancer,western medicine has no special treatment for this type of liver cancer in addition to routine treatment,whil...Liver depression and qi stagnation type liver cancer accounts for a high proportion of primary liver cancer,western medicine has no special treatment for this type of liver cancer in addition to routine treatment,while patients with this type of liver cancer often show pain in the right flank,mass under the right side,chest depression and other clinical symptoms seriously affect the quality of life of patients with liver cancer and bring great trouble to patients and their families.Chaihu Shugan San,which originated from the General order of Medicine in the Ming Dynasty,has a serious impact on the quality of life of patients with liver cancer and brings great trouble to patients and their families.It is the representative prescription of soothing the liver and regulating qi in traditional Chinese medicine.in recent years,its clinical application and pharmacological action in liver cancer have been studied more and more deeply,but the anti-tumor pharmacological research and effective components of Chaihu Shugan Powder are still lack of systematic summary.Based on the relevant studies at home and abroad,this paper summarizes the anti-tumor mechanism and possible effective components of Chaihu Shugan Powder,so as to provide further pharmacodynamic material basis and mechanism reference for the further clinical application of primary liver cancer.展开更多
Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network ph...Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network pharmacology.Methods:Absorption,distribution,metabolism,excretion,and toxicity calculation method was used to screen the active components of CSP.Traditional Chinese Medicine System Pharmacological Database Analysis Platform and text mining tool(GoPuMed database)were used to predict and screen the active ingredients of CSP and anti-depressive targets.Through Genetic Association Database,Therapeutic Target Database,and PharmGkb database targets for depression were obtained.Cytoscape3.2.1 software was used to establish a network map of the active ingredients-targets of CSP,and to analyze gene function and metabolic pathways through Database for Annotation,Visualization and Integrated Discovery and the Omicshare database.Results:The 121 active ingredients and 15 depression-related targets which were screened from the database can exert antidepressant effects by improving the neural plasticity,growth,transfer condition and gene expression of neuronal cell,and the raise of the expression of gap junction protein.The 15 targets passed 14 metabolic pathways,mainly involved in the regulation of neurotransmitters(5-hydroxytryptamine,dopamine and epinephrine),inflammatory mediator regulation of TRP channels,calcium signaling pathway,cyclic adenosine monophosphate signaling pathway and neuroactive ligand-receptor interaction and other signal channels to exert anti-depressant effects.Conclusion:This article reveals the possible mechanism of CSP in the treatment of depression through network pharmacology research,and lays a foundation for further target studies.展开更多
Objective:To investigate the antidepressant-like effects of Chaihu Shugan Powder(CSP,柴胡疏肝散)and to explore its underlying mechanisms.Methods:Thirty-two Sprague-Dawley rats were randomly divided into control(CON),c...Objective:To investigate the antidepressant-like effects of Chaihu Shugan Powder(CSP,柴胡疏肝散)and to explore its underlying mechanisms.Methods:Thirty-two Sprague-Dawley rats were randomly divided into control(CON),chronic unpredictable mild stress(CUMS),fluoxetine(FLU),and CSP groups,8 rats in each group.All of the rats except for those in the control group were subjected to 3 consecutive weeks of CUMS to establish the depression model.The open field test(OFT),forced swimming test(FST),and sucrose preference test were used to assess the anti-anxiety and antidepressant effects of CSP.Terminal deoxynucleotidyl transferase(Td T)d UTP nick-end labeling was used to determine the apoptosis rate in the hippocampal tissues.The m RNA and protein levels of glucose-regulated protein(GRP)78,spliced X-box-binding protein(XBP)-1,CCAAT/enhancerbinding protein homologous protein(CHOP),caspase-12,and c-Jun N-terminal kinase(JNK)in the hippocampus of rats were evaluated by real-time PCR and Western blot analysis,respectively.Results:Administration of CSP alleviated anxiety and depression-like behavior in CUMS rats,as revealed by enhanced time and distance in the center of the OFT(P<0.05),an increased preference for sucrose,and longer swimming time and shorter immobility time during the FST(all P<0.05).In addition,CSP treatment significantly reduced the rate of apoptosis in rat hippocampal neurons(P<0.05).The m RNA and protein expression levels of GRP78,spliced XBP-1,and CHOP were down-regulated along with the expression of caspase-12 and cleaved caspase-12 proteins(all P<0.05),whereas total and phosphorylated JNK1 protein levels did not differ significantly between control and CSP-treated rats.Conclusion:CSP can improve depression-like behavior in rats exposed to CUMS,possibly by suppressing CHOP and caspase-12 mediated apoptosis in the rat hippocampus.展开更多
基金science and Technology Project of traditional Chinese Medicine in Shandong Province(No.2020M139).
文摘Liver depression and qi stagnation type liver cancer accounts for a high proportion of primary liver cancer,western medicine has no special treatment for this type of liver cancer in addition to routine treatment,while patients with this type of liver cancer often show pain in the right flank,mass under the right side,chest depression and other clinical symptoms seriously affect the quality of life of patients with liver cancer and bring great trouble to patients and their families.Chaihu Shugan San,which originated from the General order of Medicine in the Ming Dynasty,has a serious impact on the quality of life of patients with liver cancer and brings great trouble to patients and their families.It is the representative prescription of soothing the liver and regulating qi in traditional Chinese medicine.in recent years,its clinical application and pharmacological action in liver cancer have been studied more and more deeply,but the anti-tumor pharmacological research and effective components of Chaihu Shugan Powder are still lack of systematic summary.Based on the relevant studies at home and abroad,this paper summarizes the anti-tumor mechanism and possible effective components of Chaihu Shugan Powder,so as to provide further pharmacodynamic material basis and mechanism reference for the further clinical application of primary liver cancer.
基金Supported by Jiangsu Administration of Traditional Chinese Medicine(No.YB2015052)Scientific Research Project of Jiangsu Health Commission(No.Z2018005)Jiangsu Provincial Bureau of Traditional Chinese Medicine"Chinese Medicine Encephalopathy"Key Discipline Open Project。
文摘Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network pharmacology.Methods:Absorption,distribution,metabolism,excretion,and toxicity calculation method was used to screen the active components of CSP.Traditional Chinese Medicine System Pharmacological Database Analysis Platform and text mining tool(GoPuMed database)were used to predict and screen the active ingredients of CSP and anti-depressive targets.Through Genetic Association Database,Therapeutic Target Database,and PharmGkb database targets for depression were obtained.Cytoscape3.2.1 software was used to establish a network map of the active ingredients-targets of CSP,and to analyze gene function and metabolic pathways through Database for Annotation,Visualization and Integrated Discovery and the Omicshare database.Results:The 121 active ingredients and 15 depression-related targets which were screened from the database can exert antidepressant effects by improving the neural plasticity,growth,transfer condition and gene expression of neuronal cell,and the raise of the expression of gap junction protein.The 15 targets passed 14 metabolic pathways,mainly involved in the regulation of neurotransmitters(5-hydroxytryptamine,dopamine and epinephrine),inflammatory mediator regulation of TRP channels,calcium signaling pathway,cyclic adenosine monophosphate signaling pathway and neuroactive ligand-receptor interaction and other signal channels to exert anti-depressant effects.Conclusion:This article reveals the possible mechanism of CSP in the treatment of depression through network pharmacology research,and lays a foundation for further target studies.
基金Supported by National Natural Science Foundation of China(No.81574038)Shenzhen Science and Technology Program(No.JCYJ20180508152437368)Sanming Project of Medicine in Shenzhen,China(No.SZSM201612049)。
文摘Objective:To investigate the antidepressant-like effects of Chaihu Shugan Powder(CSP,柴胡疏肝散)and to explore its underlying mechanisms.Methods:Thirty-two Sprague-Dawley rats were randomly divided into control(CON),chronic unpredictable mild stress(CUMS),fluoxetine(FLU),and CSP groups,8 rats in each group.All of the rats except for those in the control group were subjected to 3 consecutive weeks of CUMS to establish the depression model.The open field test(OFT),forced swimming test(FST),and sucrose preference test were used to assess the anti-anxiety and antidepressant effects of CSP.Terminal deoxynucleotidyl transferase(Td T)d UTP nick-end labeling was used to determine the apoptosis rate in the hippocampal tissues.The m RNA and protein levels of glucose-regulated protein(GRP)78,spliced X-box-binding protein(XBP)-1,CCAAT/enhancerbinding protein homologous protein(CHOP),caspase-12,and c-Jun N-terminal kinase(JNK)in the hippocampus of rats were evaluated by real-time PCR and Western blot analysis,respectively.Results:Administration of CSP alleviated anxiety and depression-like behavior in CUMS rats,as revealed by enhanced time and distance in the center of the OFT(P<0.05),an increased preference for sucrose,and longer swimming time and shorter immobility time during the FST(all P<0.05).In addition,CSP treatment significantly reduced the rate of apoptosis in rat hippocampal neurons(P<0.05).The m RNA and protein expression levels of GRP78,spliced XBP-1,and CHOP were down-regulated along with the expression of caspase-12 and cleaved caspase-12 proteins(all P<0.05),whereas total and phosphorylated JNK1 protein levels did not differ significantly between control and CSP-treated rats.Conclusion:CSP can improve depression-like behavior in rats exposed to CUMS,possibly by suppressing CHOP and caspase-12 mediated apoptosis in the rat hippocampus.
