The Chaihu herbal injection was the first herbal injection to be developed and used in China,which has been used in clinic for more than 70 years.This injection is widely used to treat fever caused by influenza or com...The Chaihu herbal injection was the first herbal injection to be developed and used in China,which has been used in clinic for more than 70 years.This injection is widely used to treat fever caused by influenza or common cold and malaria.However,there is an ongoing debate about the safety of the clinical use of Chaihu herbal injection in view of the large number of adverse drug reaction reports and literature in China.On May 29,2018,the China Food and Drug Administration issued a notice requiring to revise the instruction manual of Chaihu herbal injection,list"prohibit for children"under the taboo item,and add the warning"adverse reactions of this product include anaphylactic shock".The purpose of this review is to provide updated,comprehensive information on the pharmacology and adverse drug reaction of Chaihu herbal injection based on scientific literatures in the past few decades.展开更多
目的:基于网络药理学探讨柴胡-黄芩治疗慢性肾功能衰竭(chronic renal failure, CRF)的作用机制。方法:基于中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP...目的:基于网络药理学探讨柴胡-黄芩治疗慢性肾功能衰竭(chronic renal failure, CRF)的作用机制。方法:基于中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)检索柴胡、黄芩的活性成分及其相关靶点。通过在线人类孟德尔遗传数据库(online mendelian inheritance in man, OMIM)和GeneCards数据库检索CRF相关靶点。应用Draw Venn Diagram在线工具将柴胡、黄芩活性成分相关靶点与CRF相关靶点取交集并绘制韦恩图,得到的交集靶点即柴胡-黄芩治疗CRF的潜在靶点,采用Cytoscape3.8.0软件构建“活性成分-潜在靶点”网络图。采用STRING11.0数据库构建潜在靶点蛋白质相互作用(protein-protein interaction, PPI)网络,并筛选柴胡-黄芩治疗CRF的核心靶点。将潜在靶点导入Metascape平台进行基因本体(gene ontology, GO)功能富集分析中的生物学过程(biological process, BP)分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)信号通路富集分析,并采用Cytoscape 3.8.0软件构建“潜在靶点-信号通路”网络图。结果:基于TCMSP得到柴胡活性成分13种、黄芩活性成分32种及216个相关靶点。从GeneCards数据库及OMIM数据库获得CRF相关靶点2 607个,活性成分相关靶点与CRF相关靶点进行映射,得到137个柴胡-黄芩治疗CRF的潜在作用靶点。PPI网络分析得到6个核心靶点。GO功能富集分析共得到5 437个BP条目,KEGG信号通路富集分析筛选得到434条信号通路。结论:柴胡-黄芩可通过多成分、多靶点、多通路治疗CRF。展开更多
OBJECTIVE:To investigate the bioactive compounds of Chaihu(Radix Bupleuri Chinensis)(RB)on glaucomatous optic atrophy(GOA),and to study the pharmacological mechanism.METHODS:We collected information on the bioactive c...OBJECTIVE:To investigate the bioactive compounds of Chaihu(Radix Bupleuri Chinensis)(RB)on glaucomatous optic atrophy(GOA),and to study the pharmacological mechanism.METHODS:We collected information on the bioactive compounds of RB from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Targets related to bioactive compounds and GOA were also obtained.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway and network analyses were performed to investigate the potential mechanism of RB against GOA.Subsequently,the main bioactive compounds of RB and targets of GOA were docked by Autodock software.Moreover,a GOA model of retinal ganglion cells(RGCs)induced by cobalt chloride was established to verify the effect of RB on GOA.RESULTS:There were 17 main bioactive compounds and 46 key targets were screened as potential players in GOA.The compound-target network mainly contained 17 compounds and 46 corresponding targets,and the key targets consisted of interleukin-6(IL-6),hypoxia inducible factor-1α(HIF1A),Caspase-3,estrogen receptor alpha(ESR1),MYC proto-oncogene(MYC),and vascular endothelial growth factor A(VEGFA).Forty-nine significantly enriched GO terms,and 134 KEGG signaling pathways were identified(P<0.05),including HIF-1,tumor necrosis factor,VEGF,prolaction,and other signaling pathways.Molecular docking results showed that the main bioactive compounds of RB exhibited the strongest binding activity with IL-6.Furthermore,experimental validation showed that the RB extract inhibited the activity and promoted apoptosis of RGCs in a dose-dependent manner.The RB extract also suppressed the expression of Bax,Caspase-3,and Caspase-9 and regulated malonaldehyde,superoxide dismutase,and glutathione peroxide by inhibiting the IL-6/HIF-1αsignaling pathway.CONCLUSIONS:The present study provided insights into the mechanism of RB on GOA.RB mainly reverses GOA by inhibiting the IL-6/HIF-1αsignaling pathway.展开更多
柴胡是我国传统中药,其正品为伞形科植物柴胡或狭叶柴胡的干燥根。由于柴胡广泛的药理活性及临床应用范围,使得关于柴胡及其有效成分的研究受到广泛重视。柴胡皂苷a是从柴胡中分离得到的具有多种药理活性的天然化合物,在抗肿瘤、抗炎、...柴胡是我国传统中药,其正品为伞形科植物柴胡或狭叶柴胡的干燥根。由于柴胡广泛的药理活性及临床应用范围,使得关于柴胡及其有效成分的研究受到广泛重视。柴胡皂苷a是从柴胡中分离得到的具有多种药理活性的天然化合物,在抗肿瘤、抗炎、抗癫痫、保肝护肝等方面均展现出良好的应用前景。基于CNKI与ISI Web of Knowledge数据库查找及追溯了近年来关于柴胡皂苷a的最新研究文献报道,归纳总结了其药理学研究进展,以期为相关研究人员提供参考。展开更多
文摘The Chaihu herbal injection was the first herbal injection to be developed and used in China,which has been used in clinic for more than 70 years.This injection is widely used to treat fever caused by influenza or common cold and malaria.However,there is an ongoing debate about the safety of the clinical use of Chaihu herbal injection in view of the large number of adverse drug reaction reports and literature in China.On May 29,2018,the China Food and Drug Administration issued a notice requiring to revise the instruction manual of Chaihu herbal injection,list"prohibit for children"under the taboo item,and add the warning"adverse reactions of this product include anaphylactic shock".The purpose of this review is to provide updated,comprehensive information on the pharmacology and adverse drug reaction of Chaihu herbal injection based on scientific literatures in the past few decades.
文摘目的:基于网络药理学探讨柴胡-黄芩治疗慢性肾功能衰竭(chronic renal failure, CRF)的作用机制。方法:基于中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)检索柴胡、黄芩的活性成分及其相关靶点。通过在线人类孟德尔遗传数据库(online mendelian inheritance in man, OMIM)和GeneCards数据库检索CRF相关靶点。应用Draw Venn Diagram在线工具将柴胡、黄芩活性成分相关靶点与CRF相关靶点取交集并绘制韦恩图,得到的交集靶点即柴胡-黄芩治疗CRF的潜在靶点,采用Cytoscape3.8.0软件构建“活性成分-潜在靶点”网络图。采用STRING11.0数据库构建潜在靶点蛋白质相互作用(protein-protein interaction, PPI)网络,并筛选柴胡-黄芩治疗CRF的核心靶点。将潜在靶点导入Metascape平台进行基因本体(gene ontology, GO)功能富集分析中的生物学过程(biological process, BP)分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)信号通路富集分析,并采用Cytoscape 3.8.0软件构建“潜在靶点-信号通路”网络图。结果:基于TCMSP得到柴胡活性成分13种、黄芩活性成分32种及216个相关靶点。从GeneCards数据库及OMIM数据库获得CRF相关靶点2 607个,活性成分相关靶点与CRF相关靶点进行映射,得到137个柴胡-黄芩治疗CRF的潜在作用靶点。PPI网络分析得到6个核心靶点。GO功能富集分析共得到5 437个BP条目,KEGG信号通路富集分析筛选得到434条信号通路。结论:柴胡-黄芩可通过多成分、多靶点、多通路治疗CRF。
基金Natural Science Foundation of Heilongjiang Province Project:Study on the Protective Effect of Tongqiao Mingmu No.4 on Retinal RGCs in Glaucoma based on Mitochondrial Apoptosis Pathway (No.QC2018115)Special Scientific Research Project of Henan Clinical Research Base of Traditional Chinese Medicine Project:Study on the Mechanism of Tongqiao Mingmu Decoction in the Treatment of Glaucomatous Optic Nerve Atrophy Based on p53-SLC7A11 Mediated RGC Ferroptosis to Regulate Microglial Cell Polarization (No.2022ZDZX127)
文摘OBJECTIVE:To investigate the bioactive compounds of Chaihu(Radix Bupleuri Chinensis)(RB)on glaucomatous optic atrophy(GOA),and to study the pharmacological mechanism.METHODS:We collected information on the bioactive compounds of RB from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Targets related to bioactive compounds and GOA were also obtained.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway and network analyses were performed to investigate the potential mechanism of RB against GOA.Subsequently,the main bioactive compounds of RB and targets of GOA were docked by Autodock software.Moreover,a GOA model of retinal ganglion cells(RGCs)induced by cobalt chloride was established to verify the effect of RB on GOA.RESULTS:There were 17 main bioactive compounds and 46 key targets were screened as potential players in GOA.The compound-target network mainly contained 17 compounds and 46 corresponding targets,and the key targets consisted of interleukin-6(IL-6),hypoxia inducible factor-1α(HIF1A),Caspase-3,estrogen receptor alpha(ESR1),MYC proto-oncogene(MYC),and vascular endothelial growth factor A(VEGFA).Forty-nine significantly enriched GO terms,and 134 KEGG signaling pathways were identified(P<0.05),including HIF-1,tumor necrosis factor,VEGF,prolaction,and other signaling pathways.Molecular docking results showed that the main bioactive compounds of RB exhibited the strongest binding activity with IL-6.Furthermore,experimental validation showed that the RB extract inhibited the activity and promoted apoptosis of RGCs in a dose-dependent manner.The RB extract also suppressed the expression of Bax,Caspase-3,and Caspase-9 and regulated malonaldehyde,superoxide dismutase,and glutathione peroxide by inhibiting the IL-6/HIF-1αsignaling pathway.CONCLUSIONS:The present study provided insights into the mechanism of RB on GOA.RB mainly reverses GOA by inhibiting the IL-6/HIF-1αsignaling pathway.
文摘柴胡是我国传统中药,其正品为伞形科植物柴胡或狭叶柴胡的干燥根。由于柴胡广泛的药理活性及临床应用范围,使得关于柴胡及其有效成分的研究受到广泛重视。柴胡皂苷a是从柴胡中分离得到的具有多种药理活性的天然化合物,在抗肿瘤、抗炎、抗癫痫、保肝护肝等方面均展现出良好的应用前景。基于CNKI与ISI Web of Knowledge数据库查找及追溯了近年来关于柴胡皂苷a的最新研究文献报道,归纳总结了其药理学研究进展,以期为相关研究人员提供参考。
