Objective To explore the effect and mechanism of Chaihu Longgu Muli Decoction(柴胡龙骨牡蛎汤,CHLGMLD)in rats with temporal lobe epilepsy(TLE).Methods A total of 80 Sprague-Dawley(SD)male rats were randomized into cont...Objective To explore the effect and mechanism of Chaihu Longgu Muli Decoction(柴胡龙骨牡蛎汤,CHLGMLD)in rats with temporal lobe epilepsy(TLE).Methods A total of 80 Sprague-Dawley(SD)male rats were randomized into control(CON),model(MOD),carbamazepine(CBZ,0.1 g/kg),CHLGMLD low dose(CHLGMLD-L,12.5 g/kg),and high dose(CHLGMLD-H,25 g/kg)groups,with 16 rats in each group.TLE rat models were established in the four groups with the use of lithium-pilocarpine except for the CON group.After the successful establishment of TLE models,all drugs were administered through gavage,and distilled water was given to rats in the CON and MOD groups for four weeks.The frequency and duration of seizures before and after treatment were recorded for the evaluation of the alleviation degree.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression levels of miR-146a-3p and miR-146a-5p.The expression levels of toll-like receptor 4(TLR4),interleukin-1 receptor-associated kinase 1(IRAK1),tumor necrosis factor(TNF)receptor-associated factor 6(TRAF6),TAK1-binding protein(TAB),nuclear factor-kappa B(NF-κB),and interleukin-1 beta(IL-1β)in hippocampus were tested by immunofluorescence assay.Correlation analysis between the above factors and expressions of miR-146a-3p and miR-146a-5p were performed separately.Results CHLGMLD decreased the frequency(P<0.05)and duration(P<0.01)of seizures in rats.CHLGMLD down-regulated the expression levels of miR-146a-5p and miR-146a-3p(P<0.05),and inhibited the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β(P<0.01).The correlation analysis revealed that the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β were positively correlated with the expression levels of miR-146a-3p and miR-146a-5p detected by qRT-PCR,respectively(P<0.01).Conclusion CHLGMLD can inhibite the TLR4 signaling pathway by lowering the expression levels of miR-146a-3p and miR-146a-5p to alleviate hippocampal dentate gyrus inflammation in TLE rats,thus relieving seizures.展开更多
Chaihu plus Longgu Muli Decoction is the representative treatment method of harmonizing and releasing combined with easing for lesser yang disease proposed by famous physician Zhang Zhongjing.Through clinical cases,th...Chaihu plus Longgu Muli Decoction is the representative treatment method of harmonizing and releasing combined with easing for lesser yang disease proposed by famous physician Zhang Zhongjing.Through clinical cases,this paper firstly introduced the main framework of Chaihu plus Longgu Muli Decoction,to make the treatment method of harmonizing and releasing become more flexible in clinical application.Besides,it introduced the common changes in the learning and application of the decoction,to respond to various changes in the clinical diseases.展开更多
【目的】探讨定颤解郁方(由经方天麻钩藤饮合柴胡龙骨牡蛎汤化裁而来)对帕金森抑郁(depression in Parkinson’s disease,DPD)患者的临床疗效和安全性。【方法】将80例DPD肝郁风动扰神证患者随机分为单纯西药治疗组(简称西药组)和定颤...【目的】探讨定颤解郁方(由经方天麻钩藤饮合柴胡龙骨牡蛎汤化裁而来)对帕金森抑郁(depression in Parkinson’s disease,DPD)患者的临床疗效和安全性。【方法】将80例DPD肝郁风动扰神证患者随机分为单纯西药治疗组(简称西药组)和定颤解郁方联合西药治疗组(简称联合组),每组各40例。西药组给予美多芭联合盐酸普拉克索片的西医常规治疗,联合组在西药组的基础上联合定颤解郁方治疗,连续服用14 d为1个疗程,共治疗6个疗程(共12周)。观察2组患者治疗前后主要结局指标24项汉密尔顿抑郁量表(HAMD-24)评分以及次要结局指标世界运动障碍学会新版帕金森病综合评价量表(MDSUPDRS)、帕金森病睡眠障碍量表(PDSS)和汉密尔顿焦虑量表(HAMA)评分的变化情况,并评价2组患者的临床疗效(根据HAMD-24评分的改善情况判定)和安全性。【结果】(1)疗效方面,治疗12周后,联合组的总有效率为70.0%(28/40),西药组为27.5%(11/40),组间比较,联合组的疗效明显优于西药组(P<0.01)。(2)HAMD-24、HAMA、PDSS评分方面,治疗后,2组患者的HAMD-24、HAMA、PDSS评分均较治疗前明显下降(P<0.05),且联合组的下降幅度均明显优于西药组(P<0.05)。(3)MDS-UPDRS评分方面,治疗后,2组患者MDS-UPDRS的日常生活非运动症状评分及联合组的日常生活运动症状评分均较治疗前明显下降(P<0.05),而2组患者的运动检查和运动并发症评分及西药组的日常生活运动症状评分均较治疗前无明显下降(P>0.05);组间比较,联合组对日常生活非运动症状评分的下降幅度明显优于对照组(P<0.05)。(4)不良事件发生率方面,治疗过程中,2组患者的所有不良事件都是轻微的,其中联合组的不良事件发生率为12.5%(5/40),西药组为10.0%(4/40),组间比较,差异无统计学意义(P>0.05)。【结论】在西医常规治疗基础上联合定颤解郁方治疗DPD肝郁风动扰神证患者临床疗效确切,可有效改善患者的生活质量和其他非运动症状,且具有较高的安全性。定颤解郁方有望推荐为治疗DPD的有价值的替代治疗选择。展开更多
基金National Natural Science Foundation of China(81874429)Natural Science Foundation of Hunan Province(2020JJ5294)+3 种基金Traditional Chinese Medicine Science&Research Project of Hunan Province(202145)Excellent Youth Program of Hunan Education Department(21B0081)Hunan Provincial Administration of Traditional Chinese Medicine(D2022027)Changsha Natural Science Foundation of China(KQ2202255).
文摘Objective To explore the effect and mechanism of Chaihu Longgu Muli Decoction(柴胡龙骨牡蛎汤,CHLGMLD)in rats with temporal lobe epilepsy(TLE).Methods A total of 80 Sprague-Dawley(SD)male rats were randomized into control(CON),model(MOD),carbamazepine(CBZ,0.1 g/kg),CHLGMLD low dose(CHLGMLD-L,12.5 g/kg),and high dose(CHLGMLD-H,25 g/kg)groups,with 16 rats in each group.TLE rat models were established in the four groups with the use of lithium-pilocarpine except for the CON group.After the successful establishment of TLE models,all drugs were administered through gavage,and distilled water was given to rats in the CON and MOD groups for four weeks.The frequency and duration of seizures before and after treatment were recorded for the evaluation of the alleviation degree.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression levels of miR-146a-3p and miR-146a-5p.The expression levels of toll-like receptor 4(TLR4),interleukin-1 receptor-associated kinase 1(IRAK1),tumor necrosis factor(TNF)receptor-associated factor 6(TRAF6),TAK1-binding protein(TAB),nuclear factor-kappa B(NF-κB),and interleukin-1 beta(IL-1β)in hippocampus were tested by immunofluorescence assay.Correlation analysis between the above factors and expressions of miR-146a-3p and miR-146a-5p were performed separately.Results CHLGMLD decreased the frequency(P<0.05)and duration(P<0.01)of seizures in rats.CHLGMLD down-regulated the expression levels of miR-146a-5p and miR-146a-3p(P<0.05),and inhibited the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β(P<0.01).The correlation analysis revealed that the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β were positively correlated with the expression levels of miR-146a-3p and miR-146a-5p detected by qRT-PCR,respectively(P<0.01).Conclusion CHLGMLD can inhibite the TLR4 signaling pathway by lowering the expression levels of miR-146a-3p and miR-146a-5p to alleviate hippocampal dentate gyrus inflammation in TLE rats,thus relieving seizures.
文摘Chaihu plus Longgu Muli Decoction is the representative treatment method of harmonizing and releasing combined with easing for lesser yang disease proposed by famous physician Zhang Zhongjing.Through clinical cases,this paper firstly introduced the main framework of Chaihu plus Longgu Muli Decoction,to make the treatment method of harmonizing and releasing become more flexible in clinical application.Besides,it introduced the common changes in the learning and application of the decoction,to respond to various changes in the clinical diseases.
文摘【目的】探讨定颤解郁方(由经方天麻钩藤饮合柴胡龙骨牡蛎汤化裁而来)对帕金森抑郁(depression in Parkinson’s disease,DPD)患者的临床疗效和安全性。【方法】将80例DPD肝郁风动扰神证患者随机分为单纯西药治疗组(简称西药组)和定颤解郁方联合西药治疗组(简称联合组),每组各40例。西药组给予美多芭联合盐酸普拉克索片的西医常规治疗,联合组在西药组的基础上联合定颤解郁方治疗,连续服用14 d为1个疗程,共治疗6个疗程(共12周)。观察2组患者治疗前后主要结局指标24项汉密尔顿抑郁量表(HAMD-24)评分以及次要结局指标世界运动障碍学会新版帕金森病综合评价量表(MDSUPDRS)、帕金森病睡眠障碍量表(PDSS)和汉密尔顿焦虑量表(HAMA)评分的变化情况,并评价2组患者的临床疗效(根据HAMD-24评分的改善情况判定)和安全性。【结果】(1)疗效方面,治疗12周后,联合组的总有效率为70.0%(28/40),西药组为27.5%(11/40),组间比较,联合组的疗效明显优于西药组(P<0.01)。(2)HAMD-24、HAMA、PDSS评分方面,治疗后,2组患者的HAMD-24、HAMA、PDSS评分均较治疗前明显下降(P<0.05),且联合组的下降幅度均明显优于西药组(P<0.05)。(3)MDS-UPDRS评分方面,治疗后,2组患者MDS-UPDRS的日常生活非运动症状评分及联合组的日常生活运动症状评分均较治疗前明显下降(P<0.05),而2组患者的运动检查和运动并发症评分及西药组的日常生活运动症状评分均较治疗前无明显下降(P>0.05);组间比较,联合组对日常生活非运动症状评分的下降幅度明显优于对照组(P<0.05)。(4)不良事件发生率方面,治疗过程中,2组患者的所有不良事件都是轻微的,其中联合组的不良事件发生率为12.5%(5/40),西药组为10.0%(4/40),组间比较,差异无统计学意义(P>0.05)。【结论】在西医常规治疗基础上联合定颤解郁方治疗DPD肝郁风动扰神证患者临床疗效确切,可有效改善患者的生活质量和其他非运动症状,且具有较高的安全性。定颤解郁方有望推荐为治疗DPD的有价值的替代治疗选择。
