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Sustainability Assessment of Banana (Musa spp.) Yield Gap Reduction through Value Chain Development Interventions in Smallholder Farming Systems in Manicaland, Zimbabwe
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作者 Mthintwa Tawanda Hove Hlamalani Ngwenya Johan Van Niekerk 《Agricultural Sciences》 2024年第8期962-992,共31页
This study evaluated the effectiveness of donor supported agricultural value chain development projects in sustainably narrowing yield gaps for banana smallholder farmer producers in Manicaland, Zimbabwe. The study us... This study evaluated the effectiveness of donor supported agricultural value chain development projects in sustainably narrowing yield gaps for banana smallholder farmer producers in Manicaland, Zimbabwe. The study used a mixed methods research design that relied on farmers records for the quantitative analysis and focus group discussion and in-depth interviews for key experts for the qualitative aspects. A mix of regression analysis, t-tests, Pearsons’s correlation and analysis of variance statistical methods were used for the quantitative analysis while thematic response analysis was used for the qualitative engagements. The findings showed that by the end of the activity, the project had successfully narrowed the banana yield gaps to 18.66 tons per hectare working with a potential yield base of 40 tons. However, 5 years after the project closure the yield gap had fallen to 24.7 tons indicating a lack of sustainability of yield gap reduction. However, the yield gap slump does not override the fact that farmers on average were still able to sell approximately 5 tons per every 6 months after 5 years from the project closure which is beyond any poverty threshold. This suggests genuine upward economic mobility. Additionally, in analyzing what factors had the greatest influence on yield gap reduction, the access to fertilizer ranked highest where genetics, irrigation and improved agronomy followed in that respective order. The study further showed that farmers who receive value chain development support whilst at an already commercialized state tend to maintain productivity thresholds higher in comparison to those who are at a pre commercial state. This is evidenced by the fact that the pre commercial farmers had a yield reduction of 30.83 percent after 5 years of the project closure whereas the former realized a productivity growth of 7.84%. In conclusion, whilst the intervention was successful in transitioning farmers out of poverty, more investment should be made towards transitioning smallholder farmers to integrated soil fertility management practices and improvement of agronomic efficiency through stronger on field collaboration between research institutions, the government and development institutions. 展开更多
关键词 Yield Gap Value chain development Soil Health Varietal Replacement Rate Sustainability and Economic Mobility
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Quality Focused Apiculture Sector Value Chain Development in Ethiopia
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作者 Negash Bekena Juergen Greiling 《Journal of Agricultural Science and Technology(A)》 2017年第2期107-116,共10页
Ethiopia is an "apiculture country" with more than 10 million honey bee colonies. Ethiopia is also a "honey country"; the annual honey and beeswax production potentials are 550,000 and 50,000 metric tons, respecti... Ethiopia is an "apiculture country" with more than 10 million honey bee colonies. Ethiopia is also a "honey country"; the annual honey and beeswax production potentials are 550,000 and 50,000 metric tons, respectively, with about one tenth actually produced. Since 2008, Ethiopia is EU "third country" listed for the export of honey. However, the national research system identified serious bottlenecks, such as traditional smallholder level production with very low productivity and problematic supply chain; quality issues, including adulteration; lack of access to credit; constrained input supply chain and input quality issues. This paper illustrates how quality defects have been addressed in a quality focused value chain development (VCD) approach, with focus on: creating a favorable policy ground; implementing regional, national and continental multi-stakeholder-platforms (MSPs) for dialogue; information and knowledge sharing and sector advocacy; strengthening supportive sector organizations along the value chain; networking and market intelligence, including exposition and conference organization; expansion of research centers and activities; laboratory qualification for intematioual accreditation. Many components are implemented through the largest national apiculture sector development programme called Apiculture Scaling-Up Programme for Income and Rural Employment (ASPIRE), which transfers innovations to more than 30,000 beekeepers. Among others, ASPIRE was instrumental in qualifying national testing facilities, which in turn led to product quality improvement. However, more action is required in the fields of business-to-business (B2B) linkages, research, education and training, as well as in making so far voluntary practices mandatory. This also calls for full traceability and routine quality testing along the value chain. 展开更多
关键词 Ethiopia apiculture value chain development product quality.
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Solution and Application Development of the Supply Chain Management System in SINOPEC
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作者 Zhu Jianmin 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2004年第4期1-6,共6页
SINOPEC is an integrated energy and chemical company engaging in upstream,midstream and downstream operations. The business of SINOPEC covers E&P of oil and gas, refining,chemical production, as well as sales and ... SINOPEC is an integrated energy and chemical company engaging in upstream,midstream and downstream operations. The business of SINOPEC covers E&P of oil and gas, refining,chemical production, as well as sales and marketing of refined oil products. In the field of oil andnatural gas exploration and development, there are 13 upstream subsidiaries in SINOPEC. By the endof 2003, the proven reserves of crude oil were 3,257 mmbbls, and natural gas reserves totaled2,887.6 bcf. The oil and gas production in 2003 included 270.96 mmbbls of oil and 187.7 bcf ofnatural gas. In the area of middle-stream operation, SINOPEC has 33 refineries. The total capacityof crude distillation is 153.9 million tons/year, about 51% of the total distillation capacity inChina. In 2003, 123.5 million tons of crude oil was processed, around 53% of total crude processedin China. 展开更多
关键词 PIMS Solution and Application development of the Supply chain Management System in SINOPEC
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Study on the Development of the Entire Industrial Chain of Rice Production and Its Role in the Rural Revitalization of Zhejiang Province
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作者 Yanting LU Fudeng HUANG +2 位作者 Jinyue CHEN Lidong XIONG Chunshou LI 《Meteorological and Environmental Research》 CAS 2021年第2期44-47,共4页
Taking rice production in Zhejiang Province as an example,the ideas and key technologies for adjusting the development of rice industry from the perspective of the entire industrial chain are discussed.It is believed ... Taking rice production in Zhejiang Province as an example,the ideas and key technologies for adjusting the development of rice industry from the perspective of the entire industrial chain are discussed.It is believed that the development of high-quality rice should be regarded as an important guide for industrial transformation and upgrading to promote the healthy development of high-quality rice industry.The key to the development of highquality rice is the technical promotion of the entire industrial chain of rice production.It is necessary to actively promote the breeding of rice varieties with good taste quality and the improvement,demonstration and promotion of green cultivation techniques,promote the improvement,demonstration and promotion of rice storage and preservation technology and rice deep processing technology,promote the demonstration and promotion of the integrated production and marketing model of variety and brand,and promote the development of beautiful rice fields and colored special rice,so as to enhance the efficiency and competitiveness of rice industry in Zhejiang Province and help rural revitalization. 展开更多
关键词 Rice production development of the entire industrial chain Key technology Rural revitalization
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Risk Management Situation and Strategic Analysis of Supply Chains of Small and Medium Enterprises in Luzhou Liquor Industry Development Zone
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作者 Peng Xu 《International Journal of Technology Management》 2016年第7期64-67,共4页
After entering the information-based society, profound reform has happened in the business pattern of enterprises, and thecompetitive mode between traditional enterprises has been transformed into the competition betw... After entering the information-based society, profound reform has happened in the business pattern of enterprises, and thecompetitive mode between traditional enterprises has been transformed into the competition between supply chains in nowadays. The liquorindustry in Luzhou is the pillar industry of economy, which involves “Luzhou Old Cellar”, “Ichiro Liquor”and other large-scale enterpriseswith well-known brands. Besides, many small and medium-sized enterprises are developing and flourishing in Luzhou. Compared with thedevelopment of large enterprises, the development of small and medium sized enterprises has more uncertainty and risks. At present, Luzhou Cityis setting about to build Huangyi into a liquor centralized zone, which is also a clusterization strategy for industry development of Luzhou liquor.For Luzhou Liquor Zone, the implementation of supply chain management of small and medium-sized enterprises can greatly reduce the cost ofenterprises and improve effi ciency of operation. The growth and development of small and medium-sized enterprises in Luzhou Liquor Zone andthe promotion of advantages of industrial clusters are closely related to the risks of the supply chain. 展开更多
关键词 Risk Management of Supply chain Network Type Supply chain of Liquor Industry development Zone Risk Prevention ofSupply chain
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陕北黄土高原典型地质灾害发育特征及成灾模式研究——以榆林市榆阳区为例
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作者 刘凯 王新刚 +3 位作者 张培栋 曹宏远 薛晨 辜超颖 《自然灾害学报》 CSCD 北大核心 2024年第2期98-112,共15页
陕北黄土高原地区生态环境脆弱,区内滑坡等地质灾害多发,随着极端降雨频发、人类工程活动剧烈等因素的影响,区内地质灾害发育特征及发育类型有了新的变化。在充分结合已有资料的基础上,以陕北榆林市榆阳区为例开展了地质灾害详细调查和... 陕北黄土高原地区生态环境脆弱,区内滑坡等地质灾害多发,随着极端降雨频发、人类工程活动剧烈等因素的影响,区内地质灾害发育特征及发育类型有了新的变化。在充分结合已有资料的基础上,以陕北榆林市榆阳区为例开展了地质灾害详细调查和分析。结果表明:区内的地质灾害主要有滑坡、崩塌和地面塌陷,并发育黄土崩塌-泥流灾害链和采动型塌-滑灾害链。滑坡可分为黄土滑坡、黄土-红层滑坡、黄土-基岩滑坡和岩质滑坡,其中黄土滑坡诱发因素主要有降雨、开挖和河流侧蚀等,不同诱发因素下滑坡成灾模式不尽相同。区内发育的崩塌包括黄土崩塌和岩质崩塌,黄土崩塌成灾模式包括滑移式和倾倒式,岩质崩塌成灾模式为崩落式。区内煤矿开采频繁导致地面塌陷,形成了地面裂缝、塌陷错台、塌陷槽和塌陷坑频发等现象。最后,分析了区域内新出现的黄土崩塌-泥流灾害链和采动型塌-滑灾害链的形成机理。研究成果可为黄土高原地区地质灾害防治规划和保障重大工程安全与长期服役提供一定的参考。 展开更多
关键词 黄土高原 地质灾害 灾害链 发育特征 成灾模式
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短链氯化石蜡对褐牙鲆胚胎发育的毒性效应 被引量:1
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作者 崔庆奎 韩典峰 +10 位作者 丁玉竹 任传博 张信泽 赵军强 崔艳梅 姜芳 李佳蔚 孙琰晴 王景 宫向红 徐英江 《生态毒理学报》 CAS CSCD 北大核心 2023年第2期53-63,共11页
氯化石蜡(chlorinated paraffins,CPs)是一系列多氯正构烷烃的复杂混合物,其中短链氯化石蜡(short chain chlorinated paraffins,SCCPs)因具有持久性有机污染物(persistent organic pollutants,POPs)的诸多特性而被国内外学者广泛关注,... 氯化石蜡(chlorinated paraffins,CPs)是一系列多氯正构烷烃的复杂混合物,其中短链氯化石蜡(short chain chlorinated paraffins,SCCPs)因具有持久性有机污染物(persistent organic pollutants,POPs)的诸多特性而被国内外学者广泛关注,但目前关于SCCPs毒性效应的报道仍然较少。因此本研究基于SCCPs的海洋污染现状,以褐牙鲆胚胎为实验对象,通过132 h的暴露实验探究了SCCPs的毒性效应。结果表明,SCCPs具有显著的胚胎发育毒性,能够导致胚胎-仔鱼存活率降低,且具有时间效应-剂量效应;能够导致胚胎孵化延迟,高浓度(≥1000μg·L^(-1))还会导致孵化率降低;能够引起胚胎-仔鱼发育畸形,如脊柱弯曲、心包水肿等;能够抑制仔鱼生长等。此外,SCCPs还能诱导胚-仔鱼发生氧化应激反应,实验组仔鱼的超氧化物歧化酶活性、过氧化氢酶活性及丙二醛的含量均显著高于对照组(P<0.05),抗氧化酶基因sod、cat的相对表达量也要高于对照组。随着SCCPs浓度增加,实验组仔鱼超氧化物歧化酶、过氧化氢酶活性呈下降趋势,清除活性氧自由基的能力逐渐下降,导致机体发生严重的脂质过氧化作用,10000μg·L^(-1)实验组仔鱼中观察到丙二醛含量接近于对照组的3倍。研究结果揭示了SCCPs对海洋生物早期发育阶段的生态毒性,将为SCCPs的环境风险评估提供理论依据。 展开更多
关键词 短链氯化石蜡 褐牙鲆胚胎 发育毒性 氧化应激
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基于产业链的中国风电装备制造业发展策略研究 被引量:20
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作者 冯伟 李颖洁 《中国科技论坛》 CSSCI 北大核心 2010年第2期61-66,共6页
风电装备制造业作为风电产业的上游产业,已受到各国政府的普遍重视。本文基于产业链的视角,在分析国内外风电装备制造业现状的基础上,从分工协作、交易成本及产业集聚等角度剖析了我国风电装备制造业产业链发展滞后的原因,并在此基础上... 风电装备制造业作为风电产业的上游产业,已受到各国政府的普遍重视。本文基于产业链的视角,在分析国内外风电装备制造业现状的基础上,从分工协作、交易成本及产业集聚等角度剖析了我国风电装备制造业产业链发展滞后的原因,并在此基础上有针对性地提出了相应的对策建议,如坚持风电国产化、加强资源整合力度、提高产业集聚程度等。 展开更多
关键词 风电装备制造业 产业链 发展策略
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中国农产品冷链物流体系研究 被引量:22
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作者 朱超才 《安徽农业科学》 CAS 北大核心 2011年第4期2317-2318,共2页
针对我国农产品冷链物流发展中存在的标准建设滞后、基础设施不足、市场化程度低等问题,提出了完善冷链物流标准体系、大力发展基础设施、加快培育第三方冷链物流企业等对策,以促进我国农产品冷链物流的发展。
关键词 农产品 冷链物流 发展策略
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山西省农产品冷链物流需求推动力及发展策略 被引量:3
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作者 王兴华 宗颖生 +1 位作者 王广斌 丁丽芳 《山西农业科学》 2013年第12期1400-1404,1408,共6页
在阐述山西经济发展以及农产品生产现状的基础上,从冷链物流需求推动力研究入手,针对山西省农产品冷链物流发展存在的冷链流通体系不完善、农产品加工比例低、冷链物流企业培育乏力、专业人才缺乏等问题,提出了构建冷链安全追溯系统、... 在阐述山西经济发展以及农产品生产现状的基础上,从冷链物流需求推动力研究入手,针对山西省农产品冷链物流发展存在的冷链流通体系不完善、农产品加工比例低、冷链物流企业培育乏力、专业人才缺乏等问题,提出了构建冷链安全追溯系统、加大农产品加工比例、创新专业人才培养模式、利用政策优势促进冷链物流企业发展的相应对策和建议。 展开更多
关键词 山西 农产品 冷链物流 推动力 发展策略
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农产品供应链风险评估研究综述 被引量:6
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作者 刘永胜 申文娟 《物流技术》 北大核心 2013年第12期359-362,共4页
从农产品供应链风险因素分析、风险评估指标选取、风险评估方法等三个方面对近年来农产品供应链风险评估的研究成果进行了分析,指出了目前农产品供应链风险评估研究中存在的主要问题,并给出了农产品供应链风险评估研究未来的发展趋势。
关键词 农产品供应链 风险评估 研究综述 发展趋势
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社会化小农选择精准农业发展模式初探 被引量:3
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作者 赵国杰 侯建平 郭春丽 《中国农机化》 北大核心 2011年第2期24-27,共4页
精准农业有利于推动农业生产知识化与信息化进程,改变传统技术思想,追踪科技进步,改变农村经济增长方式,给社会主义新农村建设注入新的活力。基于世界精准农业的实践,探讨了精准农业实现的层级,建立精准农业矩阵结构体系;引入价值链理论... 精准农业有利于推动农业生产知识化与信息化进程,改变传统技术思想,追踪科技进步,改变农村经济增长方式,给社会主义新农村建设注入新的活力。基于世界精准农业的实践,探讨了精准农业实现的层级,建立精准农业矩阵结构体系;引入价值链理论,建构了精准农业价值链和社会化小农精准农业价值链,提出了社会化小农户实践精准农业的两种路径:松散联合体模式和农民专业合作社模式。 展开更多
关键词 社会化小农 精准农业矩阵 精准农业价值链 精准农业发展模式
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取食加Cd^(2+)食物后拟水狼蛛发育历期、耐饥力和体内Cd(2+)含量的变化 被引量:4
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作者 张征田 张虎成 +4 位作者 王庆林 庞振凌 梁子安 夏敏 杜瑞卿 《昆虫学报》 CAS CSCD 北大核心 2011年第9期997-1002,共6页
为明确食物中重金属离子在蜘蛛体内的传递、生物放大及对其生长历期和耐饥力的影响,采用原子吸收光谱法检测了拟水狼蛛Pirata subpiraticus取食加Cd2+食物后体内Cd2+含量变化情况,测定了Cd2+对其发育历期和耐饥力的间接影响。结果表明:... 为明确食物中重金属离子在蜘蛛体内的传递、生物放大及对其生长历期和耐饥力的影响,采用原子吸收光谱法检测了拟水狼蛛Pirata subpiraticus取食加Cd2+食物后体内Cd2+含量变化情况,测定了Cd2+对其发育历期和耐饥力的间接影响。结果表明:食物中的Cd2+能够通过食物链进行传递并在拟水狼蛛体内积累,积累量随拟水狼蛛龄期的增长而增加,积累量与消耗的黑腹果蝇Drosophila melanogaster数量极显著正相关(P<0.01),与消耗食物中Cd2+含量显著正相关(P<0.05);对食物中Cd2+的吸收率为65.4%,生物放大因子为1.8。成蛛改喂无Cd2+的黑腹果蝇后在观察的4周内体内Cd2+含量变化不明显。食物中的Cd2+能导致拟水狼蛛的发育历期显著延长、耐饥力显著降低。研究结果可为进一步研究环境中Cd2+沿土壤-昆虫-天敌传递、放大和生理耐受等提供理论依据。 展开更多
关键词 拟水狼蛛 食物链 重金属 积累 排泄 生物放大 发育历期
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我国农村连锁超市发展现状、问题及趋势研究 被引量:4
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作者 张蕙 《特区经济》 北大核心 2009年第12期188-190,共3页
以2005年"万村千乡市场工程"的启动为标志,我国农村连锁超市进入了快速发展的新阶段,至今已取得较大进展。但无论从发展数量还是质量看,仍有很大提升空间。本文分析了其发展现状、发展中的问题,并就其未来发展趋势进行了探寻。
关键词 农村 连锁超市 现状 趋势
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“布衣知识分子”的教育人生——刁培萼先生学术思想和精神遗产 被引量:2
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作者 朱小蔓 《南京师大学报(社会科学版)》 CSSCI 北大核心 2016年第3期5-14,共10页
刁培萼教授是一位卓有成就、富有个性的教育学专家。眼下学术圈中的许多现实,人们的疑惑与批评、坚守与挣扎、憧憬与怀想时常撞击我的心,推动我为这位素朴无华、个性执着的老师,写一点回忆和感念,它不仅是表达两年来淤积于心的哀思,更... 刁培萼教授是一位卓有成就、富有个性的教育学专家。眼下学术圈中的许多现实,人们的疑惑与批评、坚守与挣扎、憧憬与怀想时常撞击我的心,推动我为这位素朴无华、个性执着的老师,写一点回忆和感念,它不仅是表达两年来淤积于心的哀思,更是灵魂的作业。作为国内第一本《马克思主义教育哲学》《教育文化学》和《农村教育学》的著作者,他在这三个教育学分支领域的开创之功,对南师大教育学科乃至全国教育学术界均具有学科奠基的重大学术价值。他看重的是学术本有的价值,他甘愿参与建设学术共同体,他享受教书、带年轻人和做研究的无比乐趣;他别无他求,需要的就是有时间、有机会做事情;再没有什么比自由思考、从心写作、无干扰地做事让他感觉更有意义、更畅快、更高贵。《教育文化学通论》是他一生教育学术集成和最后的呼唤,也是他生命一息尚存,学术劳作不止的明证。他坚持将教育实验融贯于教育哲学研究。他的平凡一生"质本洁来还洁去"。 展开更多
关键词 刁培萼 学术思想 《马克思主义教育哲学》 《寻找发展链——教育的辩证拷问》 《教育文化学通论》
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论我国对外贸易发展方式的转变——基于全球产业价值链的视角 被引量:4
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作者 朱珍 《太原理工大学学报(社会科学版)》 2013年第4期41-45,共5页
改革开放三十多年来,我国的对外贸易量已有了一定规模的增长,但质上却没有同等程度的提升。出口贸易整体仍处于全球产业价值链的中低端,自主品牌和自主知识产权较少,对外贸易质量和效益偏低,核心竞争力较弱。制约我国对外贸易质的提升... 改革开放三十多年来,我国的对外贸易量已有了一定规模的增长,但质上却没有同等程度的提升。出口贸易整体仍处于全球产业价值链的中低端,自主品牌和自主知识产权较少,对外贸易质量和效益偏低,核心竞争力较弱。制约我国对外贸易质的提升的主要原因是欠合理的对外贸易方式,应通过提高劳动密集型出口产品的技术含量、健全自主知识产权保护体系、引进消化再创新国外先进技术等对外贸易方式的转变来提升我国对外贸易在全球产业价值链分工中的地位。 展开更多
关键词 对外贸易 发展方式 全球产业价值链
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Multi-Scale Variation Prediction of PM2.5 Concentration Based on a Monte Carlo Method
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作者 Chen Ding Guizhi Wang Qi Liu 《Journal on Big Data》 2019年第2期55-69,共15页
Haze concentration prediction,especially PM2.5,has always been a significant focus of air quality research,which is necessary to start a deep study.Aimed at predicting the monthly average concentration of PM2.5 in Bei... Haze concentration prediction,especially PM2.5,has always been a significant focus of air quality research,which is necessary to start a deep study.Aimed at predicting the monthly average concentration of PM2.5 in Beijing,a novel method based on Monte Carlo model is conducted.In order to fully exploit the value of PM2.5 data,we take logarithmic processing of the original PM2.5 data and propose two different scales of the daily concentration and the daily chain development speed of PM2.5 respectively.The results show that these data are both approximately normal distribution.On the basis of the results,a Monte Carlo method can be applied to establish a probability model of normal distribution based on two different variables and random sampling numbers can also be generated by computer.Through a large number of simulation experiments,the average monthly concentration of PM2.5 in Beijing and the general trend of PM2.5 can be obtained.By comparing the errors between the real data and the predicted data,the Monte Carlo method is reliable in predicting the PM2.5 monthly mean concentration in the area.This study also provides a feasible method that may be applied in other studies to predict other pollutants with large scale time series data. 展开更多
关键词 Monte Carlo method random sampling PM2.5 concentration chain development speed trend prediction
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供应链网络特征对企业二元性创新影响的实证研究
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作者 李晓莉 《物流技术》 北大核心 2013年第8期101-105,共5页
基于河南省156家客车供应链企业的样本数据,对供应链网络特征与二元性创新之间的关系进行了实证研究。结果表明:供应链网络成员间的认知距离、网络密度与开发性创新呈负相关,而与探索性创新呈正相关;网络中心性、网络稳定性、联系强度... 基于河南省156家客车供应链企业的样本数据,对供应链网络特征与二元性创新之间的关系进行了实证研究。结果表明:供应链网络成员间的认知距离、网络密度与开发性创新呈负相关,而与探索性创新呈正相关;网络中心性、网络稳定性、联系强度与开发性创新呈正相关,而与探索性创新呈负相关。该研究为处于供应链网络内的企业进行开发性创新和探索性创新提供了指导。 展开更多
关键词 供应链网络 二元性创新 开发性创新 探索性创新
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基于价值链重构的非站场式客运发展模式——以澳中汽为例
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作者 陈章波 《价值工程》 2007年第1期52-54,共3页
随着我国自助游的盛行,客运市场出现了非站场式客运服务。通过对澳中汽现阶段价值链的深刻剖析,提出了非站场式客运只有通过价值链重构,与旅行社、酒店结成三角联盟,才能将劣势转变为优势,形成自己的核心竞争力。
关键词 非站场式客运 价值链重构 发展模式
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斜纹夜蛾表皮蛋白基因克隆及表达谱分析 被引量:4
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作者 赵鹏 张帅 +3 位作者 赵晨晨 胡方梅 崔金杰 李绍勤 《棉花学报》 CSCD 北大核心 2020年第4期292-304,共13页
【目的】分析斜纹夜蛾不同发育阶段14个表皮蛋白(Cuticular protein, CP)基因的表达方式,为深入探究CP在斜纹夜蛾生长发育过程中的功能提供参考。【方法】利用聚合酶链反应(Polymerase chain reaction, PCR)扩增斜纹夜蛾表皮蛋白基因cDN... 【目的】分析斜纹夜蛾不同发育阶段14个表皮蛋白(Cuticular protein, CP)基因的表达方式,为深入探究CP在斜纹夜蛾生长发育过程中的功能提供参考。【方法】利用聚合酶链反应(Polymerase chain reaction, PCR)扩增斜纹夜蛾表皮蛋白基因cDNA序列,分析其核苷酸序列和相应的氨基酸序列,并使用MEGA5.2软件构建了鳞翅目系统发育树。采用逆转录PCR(Reverse transcription PCR, RT-PCR)技术检测分析表皮蛋白基因在3~6龄幼虫各组织和2日龄成虫生殖器官中的表达。【结果】克隆了14个斜纹夜蛾表皮蛋白基因(Insect cuticular protein gene, ICPG),序列分析表明斜纹夜蛾表皮蛋白具有35~36个氨基酸残基保守基序,属于几丁质结合蛋白,它们与棉铃虫的CP具有高度的序列同源性。RT-PCR结果表明,14个ICPG在3~6龄幼虫和2日龄成虫中均有表达,其相对表达量在高龄期幼虫的表皮中较高,在低龄期幼虫的脂肪体中也较高。此外,所有14个ICPG均在睾丸和卵巢中低水平表达。在蛹期,SlICPG-11和SlICPG-14相对表达量最高。【结论】克隆了14个斜纹夜蛾CP基因的cDNA。其表达表现出组织和发育阶段特异性,暗示它们在斜纹夜蛾发育中的功能不同,为控制斜纹夜蛾靶标位点的选择奠定了基础。 展开更多
关键词 斜纹夜蛾 表皮蛋白 基因克隆 逆转录聚合酶链反应(RT-PCR) 组织表达 发育阶段
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